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1. 发明授权

US10537891B2 Meniscus reducing member 有权
公开(公告)号：US10537891B2
公开(公告)日：2020-01-21
申请号：US15918798
申请日：2018-03-12
申请人： STEMCELL TECHNOLOGIES INC
发明人： Oliver Egeler , Steven Woodside
IPC分类号： B01L3/00
摘要： A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 90 degrees and less than 180 degrees.

2. 发明授权

US10017741B2 Compositions and methods for obtaining enriched mesenchymal stem cell cultures 有权
公开(公告)号：US10017741B2
公开(公告)日：2018-07-10
申请号：US14776866
申请日：2014-03-12
申请人： StemCell Technologies Inc.
发明人： Brenton John Short , Christopher Duronio
IPC分类号： C12N5/0775
摘要： The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.

3. 发明申请

US20130224857A1 METHOD OF DIFFERENTIATING STEM CELLS 有权
标题翻译：差异化干细胞的方法
公开(公告)号：US20130224857A1
公开(公告)日：2013-08-29
申请号：US13829783
申请日：2013-03-14
申请人： STEMCELL TECHNOLOGIES INC.
发明人： Alexandra A. Blak , Sharon A. Louis , Michael John Riedel
IPC分类号： C12N5/071
CPC分类号： C12N5/0608 , C12N5/0606 , C12N5/0623 , C12N2500/60 , C12N2500/90 , C12N2500/92 , C12N2500/98 , C12N2502/13 , C12N2506/02 , C12N2509/00 , C12N2533/32 , C12N2533/90
摘要： The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.
摘要翻译：本公开提供了从干细胞产生胚层的方法，包括在培养基中培养干细胞，所述培养基具有促进特异性胚层祖细胞产生的渗透压浓度范围。本公开还包括从胚芽层产生不同细胞谱系以及通过免疫学方法检测它们的方法。本公开进一步提供了用于产生，分离，培养和繁殖定型祖细胞以及从三个胚层生产分化细胞的方法。本公开还提供用于诱导三个胚层的培养基和试剂盒。

4. 发明授权

US07819029B2 Automated pipette machine 有权
标题翻译：自动移液器
公开(公告)号：US07819029B2
公开(公告)日：2010-10-26
申请号：US12164154
申请日：2008-06-30
申请人： Andrew Angus , Fred Davis , Adam Donath , Richard Grant , Dirk Kurpershoek
发明人： Andrew Angus , Fred Davis , Adam Donath , Richard Grant , Dirk Kurpershoek
IPC分类号： G01N1/14 , B01L3/02 , G01N35/10
CPC分类号： B01L3/0279 , B01L2200/023 , B01L2200/0689 , G01N35/10
摘要： There is also provided an apparatus for use on an automated pipette machine for transmitting pressure changes produced by a pump on the machine to a pipette nozzle. The apparatus comprises a housing defining a chamber a first conduit and a second conduit. The first conduit extends into the chamber and has a first opening positioned in the chamber. The first conduit is fluidically connectible to the nozzle. The second conduit extends into the chamber and has a first opening positioned in the chamber. The second conduit is fluidically connectible to the pump. The first opening of the first conduit is positioned above the first opening of the second conduit. The chamber defines at least a selected volume between the height of the first opening of the first conduit and the height of the first opening of the second conduit.
摘要翻译：还提供了一种用于自动移液器的用于将由机器上的泵产生的压力变化传递到移液管喷嘴的装置。该装置包括限定腔室的壳体，第一导管和第二导管。第一导管延伸到腔室中并且具有位于腔室中的第一开口。第一导管可流体地连接到喷嘴。第二导管延伸到腔室中并且具有位于腔室中的第一开口。第二导管可流体地连接到泵。第一导管的第一开口位于第二导管的第一开口的上方。腔室限定第一导管的第一开口的高度与第二导管的第一开口的高度之间的至少一个选定的体积。

5. 发明授权

US07723047B2 Method for the discrimination and isolation of mammary epithelial stem and colony-forming cells 有权
标题翻译：用于鉴别和分离乳腺上皮干细胞和集落形成细胞的方法
公开(公告)号：US07723047B2
公开(公告)日：2010-05-25
申请号：US11758964
申请日：2007-06-06
申请人： John Stingl , Connie J. Eaves
发明人： John Stingl , Connie J. Eaves
IPC分类号： G01N33/53 , C12N5/08
CPC分类号： C12N5/0631 , C07K16/2803 , C07K16/2842 , C07K16/289 , C07K16/2896 , C12N2501/599
摘要： The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD35 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells and the antibodies specific for CD45, Ter119, CD35 and optionally CD140a, the cell conjugates are removed and the remaining epithelial cells are then incubated with an antibody composition containing antibodies specific for CD24 and CD49f or CD24 and CD14. After formation of conjugates between the epithelial cells and the antibodies specific for CD24 and CD49f or CD24 and CD14, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated. The invention also relates to kits for carrying out this method and to the cell preparations prepared by this method.
摘要翻译：本发明涉及允许小鼠乳腺干细胞和集落形成细胞（CFC）的差异分离的改进方法。该方法涉及通过与含有对CD45，Ter119，CD35和任选的CD140a特异性抗体的抗体组合物孵育而从新解离的小鼠乳腺组织中消耗非上皮细胞。在形成非上皮乳腺细胞和对CD45，Ter119，CD35和任选的CD140a特异性抗体之间的缀合物之后，移除细胞缀合物，然后将剩余的上皮细胞与含有对CD24和CD49f特异性抗体的抗体组合物孵育，或 CD24和CD14。在形成上皮细胞和CD24和CD49f或CD24和CD14特异性抗体之间的缀合物之后，可以差异分离小鼠乳腺干和腔限制的CFC细胞。本发明还涉及用于实施该方法的试剂盒以及通过该方法制备的细胞制剂。

6. 发明授权

US07638285B2 Method of the discrimination and isolation of mammary epithelial stem and colony-forming cells 有权
标题翻译：乳腺上皮干细胞和集落形成细胞的鉴别和分离方法
公开(公告)号：US07638285B2
公开(公告)日：2009-12-29
申请号：US11447956
申请日：2006-06-07
申请人： John Stingl , Connie J. Eaves
发明人： John Stingl , Connie J. Eaves
IPC分类号： G01N33/53 , C12N5/08
CPC分类号： C12N5/0631
摘要： The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD35 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells and the antibodies specific for CD45, Ter119, CD35 and optionally CD140a, the cell conjugates are removed and the remaining epithelial cells are then incubated with an antibody composition containing antibodies specific for CD24 and CD49f. After formation of conjugates between the epithelial cells and the antibodies specific for CD24 and CD49f, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated. The invention also relates to kits for carrying out this method and to the cell preparations prepared by this method.
摘要翻译：本发明涉及允许小鼠乳腺干细胞和集落形成细胞（CFC）的差异分离的改进方法。该方法涉及通过与含有对CD45，Ter119，CD35和任选的CD140a特异性抗体的抗体组合物孵育而从新解离的小鼠乳腺组织中消耗非上皮细胞。在形成非上皮乳腺细胞和对CD45，Ter119，CD35和任选的CD140a特异性抗体之间的缀合物之后，移除细胞缀合物，然后将剩余的上皮细胞与含有对CD24和CD49f特异性抗体的抗体组合物孵育。在形成上皮细胞和CD24和CD49f特异性抗体之间的缀合物之后，可以差异分离小鼠乳腺干和腔限制的CFC细胞。本发明还涉及用于实施该方法的试剂盒以及通过该方法制备的细胞制剂。

7. 发明申请

US20090007703A1 AUTOMATED PIPETTE MACHINE 有权
公开(公告)号：US20090007703A1
公开(公告)日：2009-01-08
申请号：US12164209
申请日：2008-06-30
申请人： Andrew Angus , Fred Davis , Adam Donath , Richard Grant , Dirk Kurpershoek
发明人： Andrew Angus , Fred Davis , Adam Donath , Richard Grant , Dirk Kurpershoek
IPC分类号： G01N1/14
CPC分类号： B01L3/0279 , B01L2200/023 , B01L2200/0689 , G01N35/10
摘要： A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough. At least two seating surfaces are provided on the body, including a first seating surface and a second seating surface. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to receive and sealingly mate with a second size of pipette tip in a manner such that the first end of the passage is in fluid communication with the second size of pipette tip. There is also provided an automated pipette machine which includes a tip ejector system including an arm that is movable between a first position and a second position, wherein in the first position the arm is positioned to engage the tip during movement of the nozzle along a selected path and to prevent movement of the tip along the selected path while permitting the nozzle to move along the selected path, so that the movement of the nozzle along the selected path causes the nozzle and the tip to disengage from each other, and wherein in the second position the arm is positioned to avoid engagement with the tip during movement of the nozzle. There is also provided an apparatus for use on an automated pipette machine for transmitting pressure changes produced by a pump on the machine to a pipette nozzle. The apparatus comprises a housing defining a chamber a first conduit and a second conduit. The first conduit extends into the chamber and has a first opening positioned in the chamber. The first conduit is fluidically connectible to the nozzle. The second conduit extends into the chamber and has a first opening positioned in the chamber. The second conduit is fluidically connectible to the pump. The first opening of the first conduit is positioned above the first opening of the second conduit. The chamber defines at least a selected volume between the height of the first opening of the first conduit and the height of the first opening of the second conduit.

8. 发明申请

US20040197904A1 Method for separating cells using immunorosettes 审中-公开
标题翻译：使用免疫球蛋白分离细胞的方法
公开(公告)号：US20040197904A1
公开(公告)日：2004-10-07
申请号：US10824412
申请日：2004-04-15
申请人： StemCell Technologies Inc.
发明人： Terry Thomas , Carrie Peters , Peter Lansdorp
IPC分类号： C12N005/08 , G01N033/567
CPC分类号： G01N33/80 , A61K2039/505 , C07K16/28 , C07K16/2803 , C07K2317/31 , G01N33/5094 , Y10S436/819
摘要： The present invention relates to methods for separating cells using immunorosettes. The method involves contacting a sample containing nucleated cells and red blood cells with an antibody composition which allows immunorosettes of the nucleated cells and the red blood cells to form. The antibody composition preferably contains bifunctional antibodies or tetrameric antibody complexes.
摘要翻译：本发明涉及使用免疫球蛋白分离细胞的方法。该方法包括将含有核细胞和红细胞的样品与允许形成有核细胞和红细胞的免疫球蛋白的抗体组合物接触。抗体组合物优选含有双功能抗体或四聚体抗体复合物。

9. 发明申请

US20030185817A1 Method for separating cells using immunorosettes 有权
公开(公告)号：US20030185817A1
公开(公告)日：2003-10-02
申请号：US10310990
申请日：2002-12-06
申请人： StemCell Technologies Inc.
发明人： Terry E. Thomas , Carrie E. Peters , Peter M. Lansdorp
IPC分类号： A61K039/00 , A61K039/395 , C12N005/08
CPC分类号： C07K16/28 , A61K2039/505 , C07K16/2803 , C07K2317/31 , G01N33/5094 , G01N33/80 , Y10S436/819
摘要： The present invention relates to methods for separating cells using immunorosettes. The method involves contacting a sample containing nucleated cells and red blood cells with an antibody composition which allows immunorosettes of the nucleated cells and the red blood cells to form. The antibody composition preferably contains bifunctional antibodies or tetrameric antibody complexes.

10. 发明授权

US09970849B2 Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface 有权
公开(公告)号：US09970849B2
公开(公告)日：2018-05-15
申请号：US14601367
申请日：2015-01-21
申请人： Stemcell Technologies Inc.
发明人： Andy Isamu Kokaji
IPC分类号： G01N33/543 , G01N1/40 , G01N33/68
CPC分类号： G01N1/405 , G01N33/543 , G01N33/54326 , G01N33/6854
摘要： An improved method for the preparation of target specific surfaces and uses thereof is described. In particular, the surfaces are bound to mono-specific tetrameric antibody complexes prior to their addition to a sample containing target entities and separating them from non-target entities.

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