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    • 9. 发明授权
    • Method and system for automating sample preparation for microfluidic cryo TEM
    • 用于微流体冷冻TEM自动化样品制备的方法和系统
    • US09312095B2
    • 2016-04-12
    • US13070902
    • 2011-03-24
    • Anubhav TripathiJinkee LeeArjit Bose
    • Anubhav TripathiJinkee LeeArjit Bose
    • H01J37/20
    • H01J37/20H01J2237/2001H01J2237/208
    • A method and system is provided for automatically preparing transmission electron microscopy (TEM) samples for examination by depositing extremely small samples onto a grid without need for a blotting step. A sample liquid droplet is formed at the end of a capillary, wherein a portion of the liquid is transferred to the TEM sample grid by contact. The excess volume in the liquid droplet is then retracted by an adjacent capillary. After a predetermined time interval, the retraction capillary is moved toward the drop of the sample to remove the excess volume. As compared to a conventional machine, where the blotting procedure can deform the structure of the molecule of interest, the present invention utilizes a very low shear rate for removal of the excess sample fluid.
    • 提供了一种方法和系统,用于通过将非常小的样品沉积到网格上而无需印迹步骤,自动制备透射电子显微镜(TEM)样品进行检查。 在毛细管末端形成一个样品液滴,其中一部分液体通过接触转移到TEM样品网格。 液滴中的过剩体积然后被相邻的毛细管缩回。 在预定时间间隔之后,使收缩毛细管朝向样品的液滴移动以除去过量的体积。 与常规机器相比,其中印迹方法可能使感兴趣的分子的结构变形,本发明利用非常低的剪切速率去除多余的样品流体。