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1. 发明授权

US11578040B2 [5]Helicene derivatives as molecular reporters for diagnostic applications and methods of synthesis therefor 有权
公开(公告)号：US11578040B2
公开(公告)日：2023-02-14
申请号：US16338031
申请日：2017-09-29
申请人： NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY
发明人： Thanasat Sooksimuang , Nitsara Karoonuthaisiri , Ratthaphol Charlermroj , Somboon Sahasithiwat , Waraporn Panchan , Manlika Makornwattana , Sudtida Phuengwas , Laongdao Kangkaew
IPC分类号： G01N21/76 , C07D209/58 , C07C255/54 , C07C309/11 , G01N33/58 , A61B5/00
摘要： The present invention provides the methods of synthesis of [5]helicene compounds and the use of the said compounds conjugating with biomolecules to work as molecular reporter for diagnostic. The compounds in the present invention have the chemical structure illustrated in the formula (1): wherein G is a connecting group composes of 2 carbon atoms selected from the group consisting of ethane and ethylene; A is a separated or connected group selected from the group consisting of cyano and imide; D1 is selected from the group consisting of oxyalkanoic acid, oxyalkanal and oxyalkanesulfonate; and D2 has structure selected from the group consisting of hydroxyl, oxyalkanoic acid, oxyalkanal, alkyl oxyalkanoate, oxyalkanol and oxyalkanesulfonate. The compounds in the present invention compose of aromatic [5]helicene core comprising long it-conjugating system. The said compounds contain functional groups which able to link with biomolecules and they are soluble in water or other solvents that used in binding process with biomolecules. Moreover, owing to having proper chemical structure, the compounds in the present invention exhibit good fluorescent emission in wavelength of 425-675 nm. When the said compounds connected with biomolecules, the biomolecules give good fluorescence and can be detected under ultraviolet radiation.

2. 发明授权

US09057049B2 Transgenic plants with reduced expression of AMADH2 and elevated levels of 2-acetyl-1-pyrroline 有权
标题翻译：具有降低的AMADH2表达的转基因植物和2-乙酰基-1-吡咯啉的升高水平
公开(公告)号：US09057049B2
公开(公告)日：2015-06-16
申请号：US12961398
申请日：2010-12-06
申请人： Apichart Vanavichit , Somvong Tragoonrung , Theerayut Toojinda , Samart Wanchana , Wintai Kamolsukyunyong , Siwaret Arikit
发明人： Apichart Vanavichit , Somvong Tragoonrung , Theerayut Toojinda , Samart Wanchana , Wintai Kamolsukyunyong , Siwaret Arikit
IPC分类号： C12N9/02 , C12N15/82 , C12N15/113 , C07K14/415
CPC分类号： C12N9/0008 , C07K14/415 , C12N15/8218 , C12N15/8243
摘要： The aromatic compound 2-acetyl-1-pyrroline-is the major potent flavor component of all aromatic rice and other plants. This present invention provides transgenic plants in which 2-acetyl-1-pyrroline is synthesized at a level greater than in naturally occurring non-aromatic varieties. The transgenic plants have reduced expression of the Os2AP gene and protein, resulting in an aromatic phenotype.
摘要翻译：芳香化合物2-乙酰基-1-吡咯啉是所有芳香米和其他植物的主要有效风味成分。本发明提供了其中以比天然存在的非芳族品种大的水平合成2-乙酰基-1-吡咯啉的转基因植物。转基因植物减少Os2AP基因和蛋白质的表达，导致芳香表型。

3. 发明授权

US07838442B2 Thin film solar cell and its fabrication 有权
标题翻译：薄膜太阳能电池及其制造
公开(公告)号：US07838442B2
公开(公告)日：2010-11-23
申请号：US12247320
申请日：2008-10-08
申请人： Porponth Sichanugrist , Nirut Pingate , Decha Yotsaksri
发明人： Porponth Sichanugrist , Nirut Pingate , Decha Yotsaksri
IPC分类号： H01L21/00 , H01L31/00
CPC分类号： H01L31/076 , H01L31/0745 , H01L31/075 , H01L31/077 , H01L31/1816 , Y02E10/548
摘要： A method for producing a solar cell including the steps of forming a p-type microcrystalline silicon oxide layer on a glass substrate using a PECVD method and raw gases comprising Silane gas, Diborane gas, Hydrogen gas and Carbon Dioxide gas. The method may employ a frequency of between about 13.56-60 MHz. The PECVD method may be performed at a power density of between about 10-40 mW/cm2 and a pressure of between about 0.5-2 Torr, and with a ratio of Carbon Dioxide to Silane of between about 0.10-0.24; a ratio of Diborane to Silane of 0.10 or less, and a ratio of Silane to Hydrogen of 0.01 or less. A tandem solar cell structure may be formed by forming top and bottom layers by the method described above, and placing the top layer over the bottom layer.
摘要翻译：一种太阳能电池的制造方法，包括以下步骤：使用PECVD法在玻璃基板上形成p型微晶氧化硅层，以及包含硅烷气体，二硼烷气体，氢气和二氧化碳气体的原料气体。该方法可以采用约13.56-60MHz之间的频率。 PECVD方法可以在约10-40mW / cm 2和约0.5-2乇之间的压力和二氧化碳与硅烷的比率在约0.10-0.24之间的功率密度下进行; 二硼烷与硅烷的比例为0.10以下，硅烷与氢的比例为0.01以下。可以通过上述方法形成顶层和底层，并将顶层放置在底层上来形成串联太阳能电池结构。

4. 发明申请

US20040110122A1 Three-color reagent for measurement of CD4 positive lymphocytes by flow cytometry 审中-公开
标题翻译：用于通过流式细胞术测量CD4阳性淋巴细胞的三色试剂
公开(公告)号：US20040110122A1
公开(公告)日：2004-06-10
申请号：US10461544
申请日：2003-06-16
申请人： National Science and Technology Development Agency , Thailand Research Fund
发明人： Watchara Kasinrerk
IPC分类号： G01N033/53 , C12Q001/00
CPC分类号： G01N33/56972 , G01N33/533 , G01N2333/70514
摘要： The developed reagent is three-color immunophenotyping reagent for measurement of CD4 positive lymphocytes in peripheral blood. The reagent contains 7-aminoactinomycin D (7-AAD) which intercalates into double stranded DNA and is easily excited at 488 nm. The fluorescence emission of 7-AAD has peak at 670 nm that can be detected with FL3 detector of flow cytometer. The 7-AAD, therefore, stains white blood cells and discriminates it from red blood cells. The reagent also contains fluorescein isothiocyanate (FITC) labeled CD4 monoclonal antibody and phycoerythrin (PE) labeled CD14 monoclonal antibody which are detected with FL1 and FL2 detectors of flow cytometer, respectively. The developed reagent can be used to measure number of CD4 positive lymphocytes in lymphocyte population and monitor monocyte contamination simultaneously. This reagent therefore provides more accuracy results of CD4 positive lymphocyte enumeration.
摘要翻译：开发的试剂是用于测量外周血中CD4阳性淋巴细胞的三色免疫分型试剂。该试剂含有插入双链DNA中的7-氨基生物素D（7-AAD），并且容易在488nm激发。 7-AAD的荧光发射峰值为670 nm，可用流式细胞仪的FL3检测器检测。因此，7-AAD会染白细胞并将其与红细胞区分开来。该试剂还分别含有荧光素异硫氰酸酯（FITC）标记的CD4单克隆抗体和藻红蛋白（PE）标记的CD14单克隆抗体，分别用流式细胞仪的FL1和FL2检测器检测。开发的试剂可用于测量淋巴细胞群体中CD4阳性淋巴细胞的数量，并同时监测单核细胞污染。因此，该试剂提供CD4阳性淋巴细胞计数的更准确的结果。

5. 发明申请

US20200216943A1 FABRICATION PROCESS OF 3D-STRUCTURED SURFACE-ENHANCED RAMAN SPECTROSCOPY (SERS) SUBSTRATES BY USING A LASER MARKING MACHINE TO CREATE ROUGHNESS ON METAL SHEETS 审中-公开
公开(公告)号：US20200216943A1
公开(公告)日：2020-07-09
申请号：US16648309
申请日：2018-08-31
申请人： NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY
发明人： Pitak EIAMCHAI , Noppadon NUNTAWONG , Mati HORPRATHUM , Saksorn LIMWICHEAN , Nutthamon LIMSUWAN , Viyapol PATTHANASETTAKUL , Chanunthorn CHANANONNAWATHORN
IPC分类号： C23C14/02 , C23C14/16 , C23C14/35 , B32B15/16
摘要： The present invention provides a process of making 3D-structured SERS substrates by using a laser marking machine as part of the fabrication procedures. The 3D-structured SERS substrates in the present invention comprises of a roughened metal sheet on which noble metal nanoparticles are coated. Rough structures on the metal sheet are created by a laser marking machine. Noble metal nanoparticles are deposited onto the substrates in a magnetron sputtering system. The specific parameters involved in the settings of a laser marking machine include a laser power in a range of 1-20 W, fill spacing of 0.02-0.15 mm, speed of 1-10,000 mm/s, frequency of 20-200 kHz and repetition rate of 1-50 times. The 3D-structured SERS substrates in the present invention are able to give high enhancement of Raman signals and can detect methylene blue solution with concentration as low as 1×1O″6 M.

6. 发明申请

US20200017443A1 [5]HELICENE DERIVATIVES AS MOLECULAR REPORTERS FOR DIAGNOSTIC APPLICATIONS AND METHODS OF SYNTHESIS THEREFOR 审中-公开
公开(公告)号：US20200017443A1
公开(公告)日：2020-01-16
申请号：US16338031
申请日：2017-09-29
申请人： NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY
发明人： Thanasat SOOKSIMUANG , Nitsara KAROONUTHAISIRI , Ratthaphol CHARLERMROJ , Somboon SAHASITHIWAT , Waraporn PANCHAN , Manlika MAKORNWATTANA , Sudtida PHUENGWAS , Laongdao KANGKAEW
IPC分类号： C07D209/58 , G01N33/58 , C07C309/11 , C07C255/54
摘要： The present invention provides the methods of synthesis of [5]helicene compounds and the use of the said compounds conjugating with biomolecules to work as molecular reporter for diagnostic. The compounds in the present invention have the chemical structure illustrated in the formula (1): wherein G is a connecting group composes of 2 carbon atoms selected from the group consisting of ethane and ethylene; A is a separated or connected group selected from the group consisting of cyano and imide; D1 is selected from the group consisting of oxyalkanoic acid, oxyalkanal and oxyalkanesulfonate; and D2 has structure selected from the group consisting of hydroxyl, oxyalkanoic acid, oxyalkanal, alkyl oxyalkanoate, oxyalkanol and oxyalkanesulfonate. The compounds in the present invention compose of aromatic [5]helicene core comprising long it-conjugating system. The said compounds contain functional groups which able to link with biomolecules and they are soluble in water or other solvents that used in binding process with biomolecules. Moreover, owing to having proper chemical structure, the compounds in the present invention exhibit good fluorescent emission in wavelength of 425-675 nm. When the said compounds connected with biomolecules, the biomolecules give good fluorescence and can be detected under ultraviolet radiation.

7. 发明授权

US09611503B2 Escherichia coli cell line with thyA knockout and folA knockout 有权
公开(公告)号：US09611503B2
公开(公告)日：2017-04-04
申请号：US13996539
申请日：2012-02-09
申请人： Nattida Suwanakitti , Sastra Chaotheing , Yongyuth Yuthavong , Sumalee Kamchonwongpaisan
发明人： Nattida Suwanakitti , Sastra Chaotheing , Yongyuth Yuthavong , Sumalee Kamchonwongpaisan
IPC分类号： A61K38/00 , C12Q1/26 , C12N9/06 , C12N9/10 , C12N15/70
CPC分类号： C12Q1/26 , C12N9/003 , C12N9/1007 , C12N15/70 , C12Y201/01045
摘要： The objective of this invention is to create a double thyA folA knockout Escherichia coli (E. coli) strain for antifolate screening against DHFR of malaria and other parasites. This strain is used together with a plasmid expressing DHFR-TS from the desired pathogenic organism, which constitutes an anti-DHFR assay against the pathogenic organism of interest. The benefit of this invention is that there is no interference from either host DHFR or trimethoprim, a bacterial DHFR inhibitor. This tool is easy to use and maintain. It provides quick and reliable results as compared with conventional anti-malarial and anti-parasitic assays. This invention should facilitate discovery of new anti-DHFR compounds against malaria and other parasitic diseases.

8. 发明申请

US20160230178A1 A BACTERIAL SURROGATE FOR TESTING OF ANTIMALARIALS: THYA KNOCKOUT, FOLA KNOCKOUT, FOLP KNOCKOUT, AND FOLK KNOCKOUT BACTERIA FOR TESTING OF INHIBITION OF ANTIFOLATE PATHWAY 有权
标题翻译：用于测试抗菌药物的细菌物质：THYA KNOCKOUT，FOLA KNOCKOUT，FOLP KNOCKOUT和FOLK KNOCKOUT BACTERIA用于测试抗静电途径
公开(公告)号：US20160230178A1
公开(公告)日：2016-08-11
申请号：US15025881
申请日：2014-09-18
申请人： NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY
发明人： Nattida SUWANAKITTI , Yuwadee TALAWANICH , Yongyuth YUTHAVONG , Sumalee KAMCHONWONGPAISAN
IPC分类号： C12N15/70 , C12Q1/48
CPC分类号： C12N15/70 , C12N9/003 , C12N9/1007 , C12N9/1085 , C12N9/1235 , C12N9/93 , C12Q1/48 , G01N2333/91017 , G01N2333/91165 , G01N2333/91171
摘要： In this invention, cell lines are created for enzyme inhibitory testing of inhibitors against Plasmodium falciparum DHFR-TS and HPPK-DHPS. Provided the complementing DHFR-TS and HPPK-DHPS have sufficient activities to support growth of the surrogates in un-supplemented medium, the same surrogates could be used for screening inhibitors of targets against other parasite and pathogen species e.g. Plasmodium vivax, Trypanosoma brucei, Trypanosoma cruzi, Toxoplasma gondii or Mycobacterium tuberculosis. The cell lines in this invention are Escherichia coli strain whose thyA, folA, folK, and folP genes were disrupted using genetic knockout coupled with elimination of antibiotic resistance markers. The thyA KO, folP KO, folK KO, thyAfolA KO, folKfolP KO, thyAfolAfolP KO, thyAfolAfolK KO and thyAfolAfolKfolP KO E. coli cell lines are easy and convenient for testing single and combination drugs as plasmids bearing complementing parasite genes can be introduced simply by transformation using standard antibiotic selection.
摘要翻译：在本发明中，产生了针对恶性疟原虫DHFR-TS和HPPK-DHPS的抑制剂进行酶抑制测试的细胞系。如果补充DHFR-TS和HPPK-DHPS具有足够的活性来支持未补充培养基中替代物的生长，则相同的替代物可用于筛选针对其他寄生虫和病原体物种的靶标的抑制剂，例如间日疟原虫，布鲁斯锥虫，克氏锥虫，弓形虫或结核分枝杆菌。本发明中的细胞系是大肠杆菌菌株，其thyA，folA，folk和folP基因使用遗传敲除与消除抗生素抗性标记相结合而被破坏。 thy A O，K K，，，，，by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by by 使用标准抗生素选择进行转化。

9. 发明授权

US09285367B2 Three-color reagent for measurement of CD4 positive lymphocytes by flow cytometry 有权
标题翻译：用于通过流式细胞术测量CD4阳性淋巴细胞的三色试剂
公开(公告)号：US09285367B2
公开(公告)日：2016-03-15
申请号：US10461544
申请日：2003-06-16
申请人： Watchara Kasinrerk
发明人： Watchara Kasinrerk
IPC分类号： G01N33/533 , G01N33/569
CPC分类号： G01N33/56972 , G01N33/533 , G01N2333/70514
摘要： The developed reagent is three-color immunophenotyping reagent for measurement of CD4 positive lymphocytes in peripheral blood. The reagent contains 7-aminoactinomycin D (7-AAD) which intercalates into double stranded DNA and is easily excited at 488 nm. The fluorescence emission of 7-AAD has peak at 670 nm that can be detected with FL3 detector of flow cytometer. The 7-AAD, therefore, stains white blood cells and discriminates it from red blood cells. The reagent also contains fluorescein isothiocyanate (FITC) labeled CD4 monoclonal antibody and phycoerythrin (PE) labeled CD14 monoclonal antibody which are detected with FL1 and FL2 detectors of flow cytometer, respectively. The developed reagent can be used to measure number of CD4 positive lymphocytes in lymphocyte population and monitor monocyte contamination simultaneously. This reagent therefore provides more accuracy results of CD4 positive lymphocyte enumeration.
摘要翻译：开发的试剂是用于测量外周血中CD4阳性淋巴细胞的三色免疫分型试剂。该试剂含有插入双链DNA中的7-氨基生物素D（7-AAD），并且容易在488nm激发。 7-AAD的荧光发射峰值为670 nm，可用流式细胞仪的FL3检测器检测。因此，7-AAD会染白细胞并将其与红细胞区分开来。该试剂还分别含有荧光素异硫氰酸酯（FITC）标记的CD4单克隆抗体和藻红蛋白（PE）标记的CD14单克隆抗体，分别用流式细胞仪的FL1和FL2检测器检测。开发的试剂可用于测量淋巴细胞群体中CD4阳性淋巴细胞的数量，并同时监测单核细胞污染。因此，该试剂提供CD4阳性淋巴细胞计数的更准确的结果。

10. 发明授权

US08501893B2 Synthetic method for preparing dual curable silicone compositions 失效
标题翻译：制备双重可固化硅酮组合物的合成方法
公开(公告)号：US08501893B2
公开(公告)日：2013-08-06
申请号：US12359012
申请日：2009-01-23
申请人： Wanida Janvikul , Boonlom Thavornyutikarn , Paradorn Ngamdee
发明人： Wanida Janvikul , Boonlom Thavornyutikarn , Paradorn Ngamdee
IPC分类号： C08G77/08
CPC分类号： C08G77/14 , C08G77/44 , C08J3/243 , C08J2383/06 , C08L83/10 , C08L83/00
摘要： A synthetic method for preparing dual curable (e.g., thermal and light curable) silicone compositions is provided. The first step being a chemical modification of silanichydride bonds in cyclic siloxane monomer by the hydrosilylation reaction with acrylate-/methacrylate-containing allylic monomer. The second step being a ring-opening polymerization of the acrylate-/methacrylate-functionalized cyclic siloxane monomer with siloxane monomers using an oligosiloxane as a chain terminator.
摘要翻译：提供了一种用于制备双重可固化（例如，热和可固化）硅酮组合物的合成方法。第一步是通过与含丙烯酸酯/甲基丙烯酸酯的烯丙基单体的氢化硅烷化反应来化学改性环硅氧烷单体中的硅烷二醇键。第二步是使用低聚硅氧烷作为链终止剂使丙烯酸酯/甲基丙烯酸酯官能化的环状硅氧烷单体与硅氧烷单体的开环聚合。

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