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1. 发明申请

US20040110122A1 Three-color reagent for measurement of CD4 positive lymphocytes by flow cytometry 审中-公开
标题翻译：用于通过流式细胞术测量CD4阳性淋巴细胞的三色试剂
公开(公告)号：US20040110122A1
公开(公告)日：2004-06-10
申请号：US10461544
申请日：2003-06-16
申请人： National Science and Technology Development Agency , Thailand Research Fund
发明人： Watchara Kasinrerk
IPC分类号： G01N033/53 , C12Q001/00
CPC分类号： G01N33/56972 , G01N33/533 , G01N2333/70514
摘要： The developed reagent is three-color immunophenotyping reagent for measurement of CD4 positive lymphocytes in peripheral blood. The reagent contains 7-aminoactinomycin D (7-AAD) which intercalates into double stranded DNA and is easily excited at 488 nm. The fluorescence emission of 7-AAD has peak at 670 nm that can be detected with FL3 detector of flow cytometer. The 7-AAD, therefore, stains white blood cells and discriminates it from red blood cells. The reagent also contains fluorescein isothiocyanate (FITC) labeled CD4 monoclonal antibody and phycoerythrin (PE) labeled CD14 monoclonal antibody which are detected with FL1 and FL2 detectors of flow cytometer, respectively. The developed reagent can be used to measure number of CD4 positive lymphocytes in lymphocyte population and monitor monocyte contamination simultaneously. This reagent therefore provides more accuracy results of CD4 positive lymphocyte enumeration.
摘要翻译：开发的试剂是用于测量外周血中CD4阳性淋巴细胞的三色免疫分型试剂。该试剂含有插入双链DNA中的7-氨基生物素D（7-AAD），并且容易在488nm激发。 7-AAD的荧光发射峰值为670 nm，可用流式细胞仪的FL3检测器检测。因此，7-AAD会染白细胞并将其与红细胞区分开来。该试剂还分别含有荧光素异硫氰酸酯（FITC）标记的CD4单克隆抗体和藻红蛋白（PE）标记的CD14单克隆抗体，分别用流式细胞仪的FL1和FL2检测器检测。开发的试剂可用于测量淋巴细胞群体中CD4阳性淋巴细胞的数量，并同时监测单核细胞污染。因此，该试剂提供CD4阳性淋巴细胞计数的更准确的结果。

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