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1. 发明授权

US11168308B2 Processes for producing polyunsaturated fatty acids in transgenic organisms 有权
公开(公告)号：US11168308B2
公开(公告)日：2021-11-09
申请号：US16379935
申请日：2019-04-10
申请人： BASF PLANT SCIENCE GMBH
发明人： Jörg Bauer , Tom Wetjen
IPC分类号： C12N9/02 , A23L33/105 , A23L33/115 , A23K20/158 , A23K50/80 , A23D9/00 , C12N9/10 , C12N15/82 , C12P7/64 , C12N9/00 , A23D9/02 , A61K38/00
摘要： The present invention relates to polynucleotides from Ostreococcus lucimarinus which code for desaturases and elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides, and to the polypeptides encoded by the polynucleotides. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions.

2. 发明申请

US20210163975A1 OILS, LIPIDS AND FATTY ACIDS PRODUCED IN TRANSGENIC BRASSICA PLANT 有权
公开(公告)号：US20210163975A1
公开(公告)日：2021-06-03
申请号：US17085135
申请日：2020-10-30
申请人： BASF PLANT SCIENCE GMBH
发明人： Petra Cirpus , Joerg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
IPC分类号： C12N15/82 , C12N9/02 , C12N9/10 , C12N15/52 , C12P7/64
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypetide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

3. 发明授权

US09617557B2 Plants having increased yield-related traits by expressing a growth-regulating factor (GRF) polypeptide and method for making the same 有权
公开(公告)号：US09617557B2
公开(公告)日：2017-04-11
申请号：US12677844
申请日：2008-09-15
申请人： Christophe Reuzeau , Ana Isabel Sanz Molinero , Valerie Frankard , Ramon Serrano Salom , José Miguel Mulet Salort
发明人： Christophe Reuzeau , Ana Isabel Sanz Molinero , Valerie Frankard , Ramon Serrano Salom , José Miguel Mulet Salort
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by increasing expression in a plant of a nucleic acid encoding a GRF polypeptide. The present invention also concerns plants having increased expression of a nucleic acid encoding a GRF polypeptide, which plants have increased yield-related traits relative to control plants. The invention also provides constructs useful in the methods of the invention.

4. 发明授权

US09611441B2 Plants expressing Δ6-desaturase genes and oils from these plants containing PUFAS and method for producing unsaturated fatty acids 有权
公开(公告)号：US09611441B2
公开(公告)日：2017-04-04
申请号：US14268700
申请日：2014-05-02
申请人： BASF Plant Science GmbH
发明人： Ernst Heinz , Thomas Girke , Jodi Scheffler , Oswaldo Da Costa e Silva , Hermann Schmidt , Ulrich Zähringer , Ralf Reski
IPC分类号： C12N15/87 , C12P21/06 , C07H21/04 , C11B3/00 , C12N9/02 , C12N15/82 , C12P7/64
CPC分类号： C11B3/006 , C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472 , Y02P20/52
摘要： The present invention relates to an improved process for the preparation of unsaturated fatty acids and to a process for the preparation of triglycerides with an increased content of unsaturated fatty acids. The invention relates to the generation of transgenic microorganism, with an increased content of fatty acids, oils or lipids with Δ6 double bonds owing to the expression of a moss Δ6-desaturase. The invention furthermore relates to transgenic organisms comprising a Δ6-desaturase gene, and to the use of unsaturated fatty acids ort of the triglycerides with an increased content if unsaturated fatty acids prepared in the process.

5. 发明申请

US20170066994A9 PLANTS EXPRESSING DELTA6-DESATURASE GENES AND OILS FROM THESE PLANTS CONTAINING PUFAS AND METHOD FOR PRODUCING UNSATURATED FATTY ACIDS 有权
标题翻译：从含有PUFAS的这些植物中表达DELTA6-去饱和基因和油的植物和生产不饱和脂肪酸的方法
公开(公告)号：US20170066994A9
公开(公告)日：2017-03-09
申请号：US14268700
申请日：2014-05-02
申请人： BASF Plant Science GmbH
发明人： Ernst Heinz , Thomas Girke , Jodi Scheffler , Oswaldo Da Costa e Silva , Hermann Schmidt , Ulrich Zähringer , Ralf Reski
IPC分类号： C11B3/00
CPC分类号： C11B3/006 , C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472 , Y02P20/52
摘要： The present invention relates to an improved process for the preparation of unsaturated fatty acids and to a process for the preparation of triglycerides with an increased content of unsaturated fatty acids. The invention relates to the generation of transgenic microorganism, with an increased content of fatty acids, oils or lipids with Δ6 double bonds owing to the expression of a moss Δ6-desaturase. The invention furthermore relates to transgenic organisms comprising a Δ6-desaturase gene, and to the use of unsaturated fatty acids ort of the triglycerides with an increased content if unsaturated fatty acids prepared in the process.
摘要翻译：本发明涉及一种制备不饱和脂肪酸的改进方法以及制备具有增加的不饱和脂肪酸含量的甘油三酯的方法。本发明涉及由于苔藓Δ6-去饱和酶的表达而产生具有Δ6双键的脂肪酸，油或脂质含量增加的转基因微生物。本发明还涉及包含Δ6-去饱和酶基因的转基因生物，以及如果在该方法中制备的不饱和脂肪酸，则使用具有增加的含量的甘油三酸酯的不饱和脂肪酸。

6. 发明授权

US09234205B2 Method for increasing plant yield by expressing a nucleic acid encoding an ornithine decarboxylase polypeptide and plants expressing the same 有权
公开(公告)号：US09234205B2
公开(公告)日：2016-01-12
申请号：US12988190
申请日：2009-04-16
申请人： Yves Hatzfeld
发明人： Yves Hatzfeld
IPC分类号： C12N15/82 , C12N15/29
CPC分类号： C12N15/8261 , C12N9/88 , C12Y401/01017 , Y02A40/146
摘要： Plants having enhanced yield-related traits and a method for making the same The present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits by modulating expression in a plant of a nucleic acid encoding an Ornithine Decarboxylase (ODC) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an ODC polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. In another embodiment, the present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits, by increasing expression in a plant of a nucleic acid sequence encoding a benzothiadiazole-induced homeodomain λ_(BIHD1) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding a BIHD1 polypeptide, which plants have increased yield-related traits relative to control plants. The invention also provides constructs useful in the methods of the invention. In yet another embodiment, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits by modulating expression in a plant of a nucleic acid encoding a MYB30. The present invention also concerns plants having modulated expression of a nucleic acid encoding a MYB30 polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. In yet another embodiment, the present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a THOM (tomato homeobox) protein. The present invention also concerns plants having modulated expression of a nucleic acid encoding a THOM polypeptide, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. In a further embodiment, the present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits, by increasing expression in a plant of a nucleic acid sequence encoding a benzothiadiazole-induced Jiomeodomain 2 (BIHD2) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding a BIHD2 polypeptide, which plants have increased yield-related traits relative to control plants. The invention also provides constructs useful in the methods of the invention.

7. 发明申请

US20150299726A1 AHAS MUTANTS 审中-公开
标题翻译： AHAS MUTANT
公开(公告)号：US20150299726A1
公开(公告)日：2015-10-22
申请号：US14790279
申请日：2015-07-02
申请人： BASF PLANT SCIENCE GMBH
发明人： JOHN A. MCELVER , BIJAY SINGH
IPC分类号： C12N15/82 , A01N43/50 , C12Q1/68 , C12N9/88 , A01H1/02
CPC分类号： C12N15/8274 , A01H1/02 , A01N43/50 , C12N9/88 , C12N15/8278 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： The invention provides nucleic acids encoding mutants of the acetohydroxyacid synthase (AHAS) large subunit comprising at least two mutations, for example double and triple mutants, which are useful for producing transgenic or non-transgenic plants with improved levels of tolerance to AHAS-inhibiting herbicides. The invention also provides expression vectors, cells, plants comprising the polynucleotides encoding the AHAS large subunit double and triple mutants, plants comprising two or more AHAS large subunit single mutant polypeptides, and methods for making and using the same.
摘要翻译：本发明提供编码乙酰羟酸合酶（AHAS）大亚基的突变体的核酸，其包含至少两个突变，例如双重和三重突变体，其可用于产生具有改善的对AHAS抑制性除草剂的耐受性的转基因或非转基因植物。本发明还提供了表达载体，细胞，包含编码AHAS大亚基双重和三重突变体的多核苷酸的植物，包含两个或更多个AHAS大亚单位单突变体多肽的植物及其制备和使用方法。

8. 发明授权

US09133466B2 Method for isolation of transcription termination sequences 有权
标题翻译：分离转录终止序列的方法
公开(公告)号：US09133466B2
公开(公告)日：2015-09-15
申请号：US13894840
申请日：2013-05-15
申请人： BASF Plant Science GmbH
发明人： Hee-Sook Song , Michael Kock , Jeffrey A. Brown , Linda Patricia Loyall , Liqun Xing , Hongmei Jia , John McMillan , Lesley Ireland
IPC分类号： C12N15/82 , C07H21/04 , C12N15/10
CPC分类号： C12N15/8261 , C12N15/1051 , C12N15/82 , C12N15/8216
摘要： The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by said methods isolated from rice.
摘要翻译：本发明涉及用于鉴定和分离转录终止序列的有效的高通量方法，系统和DNA构建体。本发明进一步涉及通过从水稻中分离的所述方法鉴定的特异性终止子序列。

9. 发明授权

US08916748B2 Nucleic acid molecules encoding polypeptides involved in regulation of sugar and lipid metabolism and methods of use VIII 有权
标题翻译：编码参与糖和脂质代谢调节的多肽的核酸分子及其使用方法VIII
公开(公告)号：US08916748B2
公开(公告)日：2014-12-23
申请号：US12096301
申请日：2006-12-04
申请人： Heiko A. Haertel , Garima Bhatt
发明人： Heiko A. Haertel , Garima Bhatt
IPC分类号： C12N15/82 , A01H5/00 , C12P21/04 , C12N9/00 , C07K14/415 , C12N9/10 , C12N9/12
CPC分类号： C07K14/415 , C07K16/16 , C12N9/00 , C12N9/1051 , C12N9/1205 , C12N9/93 , C12N15/8234 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12P7/6409
摘要： This invention relates generally to nucleic acid sequences encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to Arabidopsis thaliana, Brassica napus, Glycine max and Oryza sativa nucleic acid sequences encoding sugar and lipid metabolism regulator proteins and the use of these sequences in transgenic plants. In particular, the invention is directed to methods for manipulating sugar-related compounds and for increasing oil level and altering the fatty acid composition in plants and seeds. The invention further relates to methods of using these novel plant polypeptides to stimulate plant growth and/or to increase yield and/or composition of seed storage compounds.
摘要翻译：本发明一般涉及编码与种子储存化合物在植物中的存在有关的蛋白质的核酸序列。更具体地，本发明涉及编码糖和脂质代谢调节蛋白的拟南芥，甘蓝型油菜，甘氨酸和水稻核酸序列，以及这些序列在转基因植物中的用途。特别地，本发明涉及用于操纵糖相关化合物和增加油含量和改变植物和种子中的脂肪酸组成的方法。本发明还涉及使用这些新型植物多肽刺激植物生长和/或增加种子储存化合物的产量和/或组成的方法。

10. 发明申请

US20140352003A1 PLANTS HAVING ENHANCED YIELD-RELATED TRAITS AND A METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有增强的相关旅行者的植物及其制造方法
公开(公告)号：US20140352003A1
公开(公告)日：2014-11-27
申请号：US14264723
申请日：2014-04-29
申请人： BASF Plant Science GmbH
发明人： Christophe Reuzeau , Yves Hatzfeld , Valerie Frankard , Ana Isabel Sanz Molinero
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/827
摘要： The present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by increasing expression in a plant of a nucleic acid sequence encoding a zinc finger (Znf) domain-containing A20/AN1 (Znf A20/AN1) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding a Znf A20/AN1 polypeptide, which plants have increased yield-related traits relative to control plants. The invention also provides nucleic acid sequence, polypeptide sequence, and constructs useful in the methods of the invention.
摘要翻译：本发明一般涉及分子生物学领域，涉及通过增加植物中编码含锌指（Znf）结构域的A20 / AN1（Znf A20）的核酸序列的表达来增加各种植物产量相关性状的方法 / AN1）多肽。本发明还涉及具有增加的编码Znf A20 / AN1多肽的核酸序列表达的植物，该植物相对于对照植物具有增加的产量相关性状。本发明还提供了可用于本发明方法的核酸序列，多肽序列和构建体。

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