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1. 发明申请

US20140344999A1 FAMILY OF PESTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译：杀虫蛋白家族及其使用方法
公开(公告)号：US20140344999A1
公开(公告)日：2014-11-20
申请号：US14451659
申请日：2014-08-05
申请人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
发明人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
IPC分类号： C12N15/82 , C07K14/325 , C12P21/02 , A01N37/44
CPC分类号： C12N15/8286 , A01N37/44 , C07K14/325 , C12P21/02 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号B-30955，B-30956，B-30957，B-30958，B-30942，B- 30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。

2. 发明授权

US08541366B2 Synthetic AXMI-004 delta-endotoxin genes and methods for their use 有权
标题翻译：合成AXMI-004δ-内毒素基因及其使用方法
公开(公告)号：US08541366B2
公开(公告)日：2013-09-24
申请号：US12209354
申请日：2008-09-12
申请人： Nadine Carozzi , Nalini Desai , Daniel J. Tomso , Vadim Beilinson , Theodore Kahn
发明人： Nadine Carozzi , Nalini Desai , Daniel J. Tomso , Vadim Beilinson , Theodore Kahn
IPC分类号： A01N37/18 , C07K14/325
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided, particularly synthetically-derived coding sequences. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, or 18, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 6, 7, 8, 10, 12, 14, 16, or 17, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供包含δ-内毒素多肽的编码序列的组合物，特别是合成来源的编码序列。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：9,11,13,15或18所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2，4，6，7，8，10，12，14，16或17，以及其变体和片段。

3. 发明申请

US20130133107A1 GENES ENCODING NEMATODE TOXINS 有权
标题翻译：基因编码NEMATODE毒素
公开(公告)号：US20130133107A1
公开(公告)日：2013-05-23
申请号：US13747740
申请日：2013-01-23
申请人： Athenix Corporation
发明人： Cheryl L. Peters , Brian Vande Berg , Brian Carr , Julia T. Daum , Vadim Beilinson , Sandra Volrath , Candace Poutre , Kimberly Sampson
IPC分类号： C12N15/82
CPC分类号： C12N15/8241 , C12N9/0059 , C12N9/0071 , C12N15/8285 , C12Y110/03001 , C12Y114/18001 , Y02A40/164
摘要： Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀线虫活性的组合物和方法。提供了包含杀线虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀线虫剂核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包括编码SEQ ID NO：4,5,8,9,13,14,77,48或49所示氨基酸序列的核苷酸序列，所列出的核苷酸序列在SEQ ID NO：1,2,3,6,7,10,11，12，15，45或46中，以及其变体和片段。

4. 发明授权

US08426204B2 Chlamydomonas EPSPS chloroplast transit peptide (CTP) and expression cassettes and transgenic plants utilizing the CTP 有权
标题翻译：衣原体EPSPS叶绿体转运肽（CTP）和表达盒和利用CTP的转基因植物
公开(公告)号：US08426204B2
公开(公告)日：2013-04-23
申请号：US12555175
申请日：2009-09-08
申请人： Philip E. Hammer , Vadim Beilinson , Todd K. Hinson
发明人： Philip E. Hammer , Vadim Beilinson , Todd K. Hinson
IPC分类号： A01H5/00 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8279 , C12N15/8214 , C12N15/8274
摘要： The present invention provides compositions and methods for targeting polypeptides to the chloroplasts of higher plants. Compositions include expression cassettes having a nucleotide sequence encoding a chloroplast targeting peptide (CTP) operably linked to a nucleotide sequence of interest, wherein the CTP is derived from Chlamydomonas sp. Plant transformation vectors, plants and plant cells having the CTP sequences are also encompassed, as well as variants and fragments of the CTP sequences. Methods for expressing a heterologous nucleotide sequence in a plant using the CTP sequences disclosed herein are also provided.
摘要翻译：本发明提供了将多肽靶向高等植物的叶绿体的组合物和方法。组合物包括具有编码与感兴趣的核苷酸序列可操作地连接的叶绿体靶向肽（CTP）的核苷酸序列的表达盒，其中CTP衍生自衣藻属。还包括具有CTP序列的植物转化载体，植物和植物细胞，以及CTP序列的变体和片段。还提供了使用本文公开的CTP序列在植物中表达异源核苷酸序列的方法。

5. 发明授权

US08299323B2 AXMI-010, a delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-010，一种δ-内毒素基因及其使用方法
公开(公告)号：US08299323B2
公开(公告)日：2012-10-30
申请号：US12701567
申请日：2010-02-07
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： C12P21/02 , C12N15/82 , C12N15/31 , C12N1/21
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子及其变体。

6. 发明授权

US07834249B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US07834249B2
公开(公告)日：2010-11-16
申请号：US11943801
申请日：2007-11-21
申请人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg
发明人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg
IPC分类号： C12N15/52 , C12N15/82 , C12N1/21
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33，或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

7. 发明授权

US07659376B2 Antibody that binds glyphosate resistance protein 有权
标题翻译：抗草甘膦抗性蛋白质的抗体
公开(公告)号：US07659376B2
公开(公告)日：2010-02-09
申请号：US12139408
申请日：2008-06-13
申请人： Philip E. Hammer , Nicholas B. Duck
发明人： Philip E. Hammer , Nicholas B. Duck
IPC分类号： C07K16/00
CPC分类号： C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules encoding glyphosate resistance proteins are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or the nucleotide sequence set forth in SEQ ID NO:1.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供编码抗草甘膦蛋白质的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子。

8. 发明申请

US20100005543A1 TOXIN GENES AND METHODS FOR THEIR USE 有权
标题翻译：毒素基因及其使用方法
公开(公告)号：US20100005543A1
公开(公告)日：2010-01-07
申请号：US12491396
申请日：2009-06-25
申请人： Kimberly S. Sampson , Daniel John Tomso , Shruti Agarwal , Brian McNulty , Chris Campbell
发明人： Kimberly S. Sampson , Daniel John Tomso , Shruti Agarwal , Brian McNulty , Chris Campbell
IPC分类号： C12N15/82 , C12N15/32 , C12N1/21 , C07K14/325 , C07K16/12 , A01N37/18 , C12P21/02 , A01H5/00 , C12N5/10 , A01H5/10 , A01P7/04
CPC分类号： C07K14/325 , A01N37/18 , C07K16/1278 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:61-121 and 133-141, or the nucleotide sequence set forth in SEQ ID NO:1-60, 124-132, and 142-283, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：61-121和133-141所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-60,124所示的核苷酸序列 -132和142-283，以及其变体和片段。

9. 发明申请

US20100004176A1 AXMI-115, AXMI-113, AXMI-005, AXMI-163 AND AXMI-184: INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 有权
标题翻译： AXMI-115，AXMI-113，AXMI-005，AXMI-163和AXMI-184：杀虫蛋白及其使用方法
公开(公告)号：US20100004176A1
公开(公告)日：2010-01-07
申请号：US12497221
申请日：2009-07-02
申请人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
IPC分类号： A61K38/16 , C07H21/04 , C12N15/63 , C12N5/00 , C07K14/00 , C07K16/00
CPC分类号： C12N15/8286 , A01N37/18 , C07K14/32 , C07K14/325 , C07K16/1278 , Y02A40/162
摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予宿主细胞杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在宿主细胞中转化和表达。组合物还包含转化的宿主细胞。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4,5,6,13或14所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2,3,11或12，以及其变体和片段。

10. 发明申请

US20090241219A1 GDC-2 GENES CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译： GDC-2基因对抗药性的抵抗
公开(公告)号：US20090241219A1
公开(公告)日：2009-09-24
申请号：US12423926
申请日：2009-04-15
申请人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck
发明人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , C12N9/14 , C12P21/02
CPC分类号： C12N9/88 , C12N15/8275 , C12N15/8278
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3 or the nucleotide sequences set forth in SEQ ID NOS:1 and 2.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3所示的氨基酸序列的核苷酸序列或SEQ ID NO：1和2所示的核苷酸序列。

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