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81. 发明授权

US06306631B1 PPGPP synthetase and expression systems for improved production of protein of interest 有权
标题翻译： PPGPP合成酶和表达系统，用于改善感兴趣的蛋白质的产生
公开(公告)号：US06306631B1
公开(公告)日：2001-10-23
申请号：US09442055
申请日：1999-11-16
申请人： Jens Tonne Andersen , Stanislas Dusko Ehrlich
发明人： Jens Tonne Andersen , Stanislas Dusko Ehrlich
IPC分类号： C12N900
CPC分类号： C12N9/1235
摘要： The present invention relates to a novel ppGpp synthetase and expression systems for improved production of proteins of interest in Gram-positive bacteria, involving use of the novel ppGpp synthetase.
摘要翻译：本发明涉及一种新颖的ppGpp合成酶和表达系统，用于改进革兰氏阳性菌中感兴趣的蛋白质的生产，涉及使用新颖的ppGpp合成酶。

82. 发明授权

US06291219B1 &agr;1-6 fucosyltransferase 失效
标题翻译： α1-6岩藻糖基转移酶
公开(公告)号：US06291219B1
公开(公告)日：2001-09-18
申请号：US09442629
申请日：1999-11-18
申请人： Naoyuki Taniguchi , Naofumi Uozumi , Tetsuo Shiba , Shusaku Yanagidani
发明人： Naoyuki Taniguchi , Naofumi Uozumi , Tetsuo Shiba , Shusaku Yanagidani
IPC分类号： C12N900
CPC分类号： C12N9/1051
摘要： Porcine- or human-derived &agr;1-6 fucosyltransferases having the following action: action: transferring fucose from guanosine diphosphate-fucose to the hydroxy group at 6-position of GluNAc closest to R of a receptor (GlcNAc&bgr;1-2Man&agr;1-6) (GlcNAc&bgr;1-2Man&agr;1-3) Man&bgr;1-4GlcNAc&bgr;1-4GlucNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc&bgr;1-2Man&agr;1-6)-(GlcNAc&bgr;1-2Man&agr;1-3)Man&bgr;1-4GlcNAc&bgr;1-4(Fuc&agr;1-6)GlucNAc-R; a gene encoding these enzymes; an expression vector containing the gene; a transformant prepared by using this expression vector; and a method for producing a recombinant &agr;1-6 fucosyltransferase, by culturing the transformant.
摘要翻译：具有以下作用的猪或人来源的α1-6岩藻糖基转移酶：作用：将岩藻糖从鸟苷二磷酸岩藻糖转移到最接近受体R的GluNAc的6位羟基（GlcNAcbeta1-2Manalpha1-6）（GlcNAcβ1- 2Manalpha1-3）Manbeta1-4GlcNAcbeta1-4GlucNAc-R其中R是携带所述残基的天冬酰胺残基或肽链，由此形成（GlcNAcbeta1-2Manalpha1-6） - （GlcNAcbeta1-2Manalpha1-3）Manbeta1-4GlcNAcbeta1-4（Fucalpha1 -6）GlucNAc-R; 编码这些酶的基因; 含有该基因的表达载体; 通过使用该表达载体制备的转化体; 以及通过培养转化体来制备重组α1-6岩藻糖基转移酶的方法。

83. 发明授权

US06271441B1 Plant aminoacyl-tRNA synthetase 有权
公开(公告)号：US06271441B1
公开(公告)日：2001-08-07
申请号：US09357251
申请日：1999-07-20
申请人： Saverio Carl Falco , Layo O. Famodu , Emil M. Orozco, Jr. , James S. Schwaber
发明人： Saverio Carl Falco , Layo O. Famodu , Emil M. Orozco, Jr. , James S. Schwaber
IPC分类号： C12N900
CPC分类号： C12N9/93 , C12N15/8274
摘要： This invention relates to an isolated nucleic acid fragment encoding an aminoacyl-tRNA synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the aminoacyl-tRNA synthetase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the aminoacyl-tRNA synthetase in a transformed host cell.

84. 发明授权

US06271009B1 Control of fruit ripening and senescence in plants 失效
标题翻译：控制植物中的果实成熟和衰老
公开(公告)号：US06271009B1
公开(公告)日：2001-08-07
申请号：US09245736
申请日：1999-02-08
申请人： Ehud Keinan , Harel Itzhaky , Esther Aboud-Pirak , Shimon Gepstein
发明人： Ehud Keinan , Harel Itzhaky , Esther Aboud-Pirak , Shimon Gepstein
IPC分类号： C12N900
CPC分类号： C12N15/8258 , C12N9/0002 , C12N15/8249
摘要： Hapten and antigen designed for eliciting catalytic antibodies effective in inhibiting the ethylene production pathway in plants by deactivating a precursor thereof either by decomposition or derivatization. Catalytic antibodies effective in inhibiting the ethylene production pathway in plants by deactivating a precursor thereof. Genes encoding for such catalytic antibodies and plants and cells expressing these genes and producing the catalytic antibodies for controlling the ripening of fruits and vegetables, as well as for controlling senescence of plant tissue.
摘要翻译：设计用于引发催化抗体的抗原和抗原，其通过分解或衍生化使其前体失活而有效抑制植物中的乙烯生产途径。催化抗体通过使其前体失活而有效抑制植物中的乙烯生产途径。编码这种催化抗体的基因和表达这些基因的植物和细胞，并产生用于控制水果和蔬菜成熟的催化抗体，以及用于控制植物组织的衰老。

85. 发明授权

US06268138B1 Retroviral vector capable of transducing the aldehyde dehydrogenase-1 gene and making cells resistant to the chemotherapeutic agent cyclophosphamide and its derivatives and analogs 失效
标题翻译：能够转导醛脱氢酶-1基因并使细胞对化学治疗剂环磷酰胺及其衍生物和类似物具有抗性的逆转录病毒载体
公开(公告)号：US06268138B1
公开(公告)日：2001-07-31
申请号：US09221294
申请日：1998-12-23
申请人： Riccardo Dalla-Favera , Alessandro Massimo Gianni
发明人： Riccardo Dalla-Favera , Alessandro Massimo Gianni
IPC分类号： C12N900
CPC分类号： C12N15/8509 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2267/02 , C12N9/0008 , C12N9/93 , C12N15/86 , C12N2740/13043 , C12Y102/01003
摘要： This invention provides viral and retroviral vectors which comprises a nucleic acid molecule encoding a human cytosolic aldehyde dehydrogenase or a glutamylcysteine synthetase or combinations thereof. Further, this invention provides an isolated mammalian nucleic acid molecule encoding an cytosolic aldehyde dehydrogenase and glutamylcysteine synthetase. In addition, this invention provides a method for reducing the toxic effects of a cyclophosphamide in a subject which comprises replacing the subject's hematopoietic cells with hematopoietic cells of having the retroviral vector. Further, this invention provides a method for introducing a selectable marker into a mammalian cell which comprises transfecting the cell with a nucleic acid molecule encoding human cytosolic aldehyde dehydrogenase or glutamylcysteine synthetase. Lastly, this invention provides a method for selecting mammalian cells expressing protein of interest which comprises: a). introducing into the cells a nucleic acid molecule comprising a nucleic acid molecule encoding the protein of interest and the nucleic acid molecule encoding human cytosolic aldehyde dehydrogenase; b.) culturing the resulting transfected cells; and c.) selecting cells which express human cytosolic aldehyde dehydrogenase.
摘要翻译：本发明提供病毒和逆转录病毒载体，其包含编码人细胞溶质醛脱氢酶或谷氨酰半胱氨酸合成酶的核酸分子或其组合。此外，本发明提供了编码细胞溶质醛脱氢酶和谷氨酰半胱氨酸合成酶的分离的哺乳动物核酸分子。此外，本发明提供了降低受试者中环磷酰胺的毒性作用的方法，其包括用造血细胞代替受试者的造血细胞此外，本发明提供了将选择标记引入哺乳动物细胞的方法，其包括用编码人细胞溶质醛脱氢酶或谷氨酰半胱氨酸合成酶的核酸分子转染细胞。最后，本发明提供了选择哺乳动物的方法表达目的蛋白质的细胞，其包含：a）。向细胞中引入包含编码目标蛋白的核酸分子和编码人胞浆醛脱氢酶的核酸分子的核酸分子; b）培养所得转染细胞; 和c）选择表达人胞浆醛脱氢酶的细胞。

86. 发明授权

US06251646B1 Method for stabilizing nitrilase activity and preserving microbial cells 有权
标题翻译：稳定腈水解酶活性和保存微生物细胞的方法
公开(公告)号：US06251646B1
公开(公告)日：2001-06-26
申请号：US09352015
申请日：1999-07-12
申请人： Robert Dicosimo , Arie Ben-Bassat , Robert D. Fallon
发明人： Robert Dicosimo , Arie Ben-Bassat , Robert D. Fallon
IPC分类号： C12N900
CPC分类号： C12P13/00 , C12N1/04 , C12N9/78 , C12N9/96 , C12N11/04 , C12N11/08 , C12N11/10
摘要： A method for the stabilization of nitrilase activity of unimmobilized or immobilized microbial cells has been developed. The unimmobilized or immobilized microbial cells are stored in an aqueous solution containing from 0.100 M to the saturation concentration of an inorganic salt of bicarbonate or carbonate, including ammonium, sodium and potassium salts of bicarbonate or carbonate. Aqueous suspensions containing at least 100 mM bicarbonate or carbonate limit microbial contamination of the stored enzyme catalyst, as well as stabilize the desired nitrilase activity of the unimmobilized or immobilized cells. Microorganisms which are characterized by an nitrilase activity and are stabilized and preserved by this method include Acidovorax facilis 72-PF-15 (ATCC 55747), Acidovorax facilis 72-PF-17 (ATCC 55745), and Acidovorax facilis 72W (ATCC 55746).
摘要翻译：已经开发了用于稳定未固定或固定的微生物细胞的腈水解酶活性的方法。将未固定或固定的微生物细胞储存在含有0.100M的水溶液中，使饱和浓度为碳酸氢盐或碳酸盐的无机盐，包括碳酸氢盐或碳酸盐的铵，钠和钾盐。含有至少100mM碳酸氢盐或碳酸氢盐的水性悬浮液限制所储存的酶催化剂的微生物污染物，以及稳定未固化或固定的细胞的所需腈水解酶活性。特征在于腈水解酶活性并通过该方法稳定和保存的微生物包括Acidovorax facilis 72-PF-15（ATCC 55747），Acidovorax facilis 72-PF-17（ATCC 55745）和Acidovorax facilis 72W（ATCC 55746）。

87. 发明授权

US06242237B1 Enzyme with galactanase activity 有权
标题翻译：具半乳聚糖酶活性的酶
公开(公告)号：US06242237B1
公开(公告)日：2001-06-05
申请号：US09137855
申请日：1998-08-21
申请人： Lene Venke Kofod , Markus Sakari Kauppinen , Lene Nonboe Andersen , Ib Groth Clausen , Anette Müllertz
发明人： Lene Venke Kofod , Markus Sakari Kauppinen , Lene Nonboe Andersen , Ib Groth Clausen , Anette Müllertz
IPC分类号： C12N900
CPC分类号： C12Y302/01089 , A23K20/189 , A23L2/84 , A23L29/06 , C12G1/0203 , C12N9/2468
摘要： The present invention relates to an enzyme with galactanase activity, a DNA construct encoding the enzyme with galactanase activity, a method of producing the enzyme, an enzyme composition comprising the enzyme with galactanase activity, and the use of the galactanase enzyme and enzyme composition for a number of industrial applications.
摘要翻译：本发明涉及具有半乳糖酶活性的酶，编码具有半乳糖酶活性的酶的DNA构建体，该酶的制备方法，含有半乳聚糖酶活性的酶的酶组合物，以及半乳聚糖酶和酶组合物用于工业应用数量。

88. 发明授权

US06238881B1 Nucleic acids and polypeptides related to a guanine exchange factor of Rho GTPase 失效
标题翻译：与Rho GTP酶的鸟嘌呤交换因子相关的核酸和多肽
公开(公告)号：US06238881B1
公开(公告)日：2001-05-29
申请号：US08943768
申请日：1997-10-06
申请人： Matthew J. Hart
发明人： Matthew J. Hart
IPC分类号： C12N900
CPC分类号： C07K16/18 , A61K38/00 , A61K48/00 , C07K14/82 , C12Q1/6876
摘要： The present invention relates to all aspects of a guanine exchange factor (GEF), for example, a Rho-GEF, such as p115 Rho-GEF. A GEF modulates cell signaling pathways, both in in vitro and in vivo, by modulating the activity of a GTPase. By way of illustration, a p115 Rho-GEF, which modulates the activity of a Rho GTPase, is described. However, the present invention relates to other GEFs, especially other Rho-GEFs. The present invention particularly relates to an isolated p115 Rho-GEF polypeptide or fragments of it, a nucleic acid coding for p115 Rho-GEF or fragments of it, and derivatives of the polypeptide and nucleic acid. The invention also relates to methods of using such polypeptides, nucleic acids, or derivatives thereof, e.g., in therapeutics, diagnostics, and as research tools. Another aspect of the present invention involves antibodies and other ligands which recognize p115 Rho-GEF, regulators of p115 Rho-GEF activity, and methods of treating pathological conditions associated or related to a Rho GTPase.
摘要翻译：本发明涉及鸟嘌呤交换因子（GEF），例如Rho-GEF，例如p15Rho-GEF的所有方面。通过调节GTP酶的活性，GEF在体外和体内调节细胞信号通路。作为说明，描述了调节Rho GTP酶的活性的p115Rho-GEF。然而，本发明涉及其他GEF，特别是其他Rho-GEF。本发明特别涉及分离的p115Rho-GEF多肽或其片段，编码p115Rho-GEF或其片段的核酸，以及多肽和核酸的衍生物。本发明还涉及使用此类多肽，核酸或其衍生物的方法，例如在治疗学，诊断学和研究工具中。本发明的另一方面涉及识别p115Rho-GEF，p115Rho-GEF活性的调节剂以及治疗与Rho GTP酶相关或相关的病理学病症的方法的抗体和其它配体。

89. 发明授权

US06232106B1 DNA sequence encoding enzymes of clavulanic acid biosynthesis 失效
标题翻译：编码克拉维酸生物合成酶的DNA序列
公开(公告)号：US06232106B1
公开(公告)日：2001-05-15
申请号：US09385028
申请日：1999-08-30
申请人： Susan E. Jensen , Kwamena A. Aidoo , Ashish S. Paradkar
发明人： Susan E. Jensen , Kwamena A. Aidoo , Ashish S. Paradkar
IPC分类号： C12N900
CPC分类号： C07K14/36 , C12N15/52 , C12N15/76 , C12P17/188
摘要： DNA sequences are provided which encode the enzymes required for clavulanic acid synthesis. A process is provided for producing clavulanic acid in a transformant of a non-clavulanate-producing host.
摘要翻译：提供编码克拉维酸合成所需的酶的DNA序列。提供了用于在非克拉维酸盐生产宿主的转化体中产生克拉维酸的方法。

90. 发明授权

US06210941B1 Methods for the detection and isolation of proteins 有权
标题翻译：检测和分离蛋白质的方法
公开(公告)号：US06210941B1
公开(公告)日：2001-04-03
申请号：US09290325
申请日：1999-04-12
申请人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
发明人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
IPC分类号： C12N900
CPC分类号： A61K41/0042 , A61K38/00 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6883 , G01N33/58
摘要： The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may be used for the detection of diseases and disorders.
摘要翻译：本发明涉及用于在细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物，或由蛋白质合成机理识别的物质。标记可以包含可切割部分，可检测标记，报告基因特性，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区别。本发明还包括使用可用于治疗人类和其他哺乳动物疾病和病症的药物组合物中的使用错氨基酰化tRNA制备的蛋白质，以及可用于检测疾病和病症的试剂盒。

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