专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

81. 发明授权

US09562809B2 Biopolymer optical analysis device and method 有权
标题翻译：生物聚合物光学分析装置及方法
公开(公告)号：US09562809B2
公开(公告)日：2017-02-07
申请号：US13812801
申请日：2011-07-21
申请人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
发明人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
IPC分类号： G01J3/02 , B82Y5/00 , B82Y15/00 , G01N33/487 , G01N21/65
CPC分类号： G01J3/0267 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , G01N21/658 , G01N33/48721 , C12Q2565/631 , C12Q2565/632
摘要： The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 130b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a, 130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.
摘要翻译：本发明提供一种使用简单的装置结构分析具有优异机械稳定性，高空间分辨率和灵敏度的生物聚合物的特性的装置和方法。具体地，测量生物聚合物的拉曼散射光，并通过使用生物聚合物性质分析芯片（100a）分析形成生物聚合物的单体单元的性质，其特征在于包括：固体基材（110）; 设置在所述固体基板（110）中的至少一个纳米孔（120）; 以及设置在固体基板（110）上的一个或多个导电薄膜（130a，130b）。生物聚合物性质分析芯片（100a）的特征在于，导电薄膜（130a，130b）部分地设置在形成有纳米孔（120）的固体基板（110）上，并且已经渗透到纳米孔中的生物聚合物 120）通过外部光照射产生拉曼散射光。

82. 发明申请

US20170022556A1 POLYNUCLEOTIDE SEQUENCING METHODS 审中-公开
标题翻译：多核苷酸序列方法
公开(公告)号：US20170022556A1
公开(公告)日：2017-01-26
申请号：US15280636
申请日：2016-09-29
申请人： GEN-PROBE INCORPORATED
发明人： Daniel Henry DENSHAM
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2565/632 , C12Q2533/101 , C12Q2521/543
摘要： Methods for determining the sequence of a polynucleotide comprising the steps of (i) contacting a polynucleotide processive enzyme immobilised in a fixed position, with a target polynucleotide under conditions sufficient to induce enzyme activity; (ii) detecting an effect consequent on the interaction of the enzyme and the polynucleotide, wherein the effect is detected by measurement of a non-linear optical signal or a linear signal coupled to a non-linear signal.
摘要翻译：用于确定多核苷酸序列的方法，包括以下步骤：（i）将固定在固定位置的多核苷酸进程酶与靶多核苷酸在足以诱导酶活性的条件下接触; （ii）检测由酶和多核苷酸的相互作用产生的影响，其中通过测量非线性光信号或耦合到非线性信号的线性信号来检测效果。

83. 发明授权

US09494582B2 Aptamers for detection and measurement of analytes 有权
标题翻译：用于检测和测量分析物的用户
公开(公告)号：US09494582B2
公开(公告)日：2016-11-15
申请号：US14448511
申请日：2014-07-31
申请人： The University of Toledo
发明人： Brent D. Cameron , Dong-Shik Kim
IPC分类号： C07H21/02 , G01N33/543 , G01N33/53 , C12N15/115 , B05D1/18 , C12Q1/68 , A61K48/00 , C12N15/11
CPC分类号： G01N33/54353 , B05D1/18 , C12N15/115 , C12N2310/16 , C12N2320/10 , C12N2330/31 , C12Q1/6825 , G01N21/658 , G01N33/5308 , G01N33/54306 , G01N33/54373 , G01N33/54393 , G01N33/68 , G01N33/721 , G01N33/723 , G01N2333/76 , G01N2333/765 , G01N2333/805 , G01N2440/38 , G01N2610/00 , C12Q2525/205 , C12Q2565/628 , C12Q2565/601 , C12Q2565/632
摘要： Sensors for target entities having functionalized thereon, at least one aptamer specific to the target entity, and methods of making and using the same are described for use in glycated protein monitoring and/or biomarkers.
摘要翻译：用于在其上官能化的目标实体的传感器，对靶实体特异性的至少一个适配体及其制备和使用该靶的实体的方法描述用于糖基化蛋白质监测和/或生物标志物。

84. 发明授权

US09417234B2 Methods and devices for detection and measurement of analytes 有权
标题翻译：用于检测和测量分析物的方法和装置
公开(公告)号：US09417234B2
公开(公告)日：2016-08-16
申请号：US14375929
申请日：2013-01-31
申请人： The University of Toledo
发明人： Brent D. Cameron , Dong-Shik Kim
IPC分类号： G01N33/543 , G01N33/53 , C12N15/115 , B05D1/18 , C12Q1/68
CPC分类号： G01N33/54353 , B05D1/18 , C12N15/115 , C12N2310/16 , C12N2320/10 , C12N2330/31 , C12Q1/6825 , G01N21/658 , G01N33/5308 , G01N33/54306 , G01N33/54373 , G01N33/54393 , G01N33/68 , G01N33/721 , G01N33/723 , G01N2333/76 , G01N2333/765 , G01N2333/805 , G01N2440/38 , G01N2610/00 , C12Q2525/205 , C12Q2565/628 , C12Q2565/601 , C12Q2565/632
摘要： Sensors for target entities having functionalized thereon, at least one aptamer specific to the target entity, and methods of making and using the same are described for use in glycated protein monitoring and/or biomarkers.
摘要翻译：用于在其上官能化的目标实体的传感器，对靶实体特异性的至少一个适配体及其制备和使用该靶的实体的方法描述用于糖基化蛋白质监测和/或生物标志物。

85. 发明授权

US09006458B2 Surface Enhanced Raman Spectroscopy (SERS) compounds and methods of their preparation 有权
标题翻译：表面增强拉曼光谱（SERS）化合物及其制备方法
公开(公告)号：US09006458B2
公开(公告)日：2015-04-14
申请号：US13272187
申请日：2011-10-12
申请人： Young-Tae Chang , Kaustabh Kumar Maiti , Dinish Unnimadhava Kurup Soudamini Amma , Chit Yaw Fu , Malini Olivo , Kiat Seng Jason Soh , Seong-Wook Yun
发明人： Young-Tae Chang , Kaustabh Kumar Maiti , Dinish Unnimadhava Kurup Soudamini Amma , Chit Yaw Fu , Malini Olivo , Kiat Seng Jason Soh , Seong-Wook Yun
IPC分类号： C07D339/04 , C07D409/10 , C12Q1/68 , A61K49/00 , B82Y15/00 , C07C251/30 , C07D295/135 , C07D409/12 , G01N33/533
CPC分类号： C12Q1/6816 , A61K49/0065 , B82Y15/00 , C07C251/30 , C07D295/135 , C07D339/04 , C07D409/12 , G01N33/533 , C12Q2563/155 , C12Q2565/632
摘要： A compound for detecting an analyte using Surface Enhanced Raman Spectroscopy (SERS) and a method of forming the compound is provided. The compound has Formula I: wherein W is selected from the group consisting of an optionally substituted aryl group and an optionally substituted heteroaryl group; each Y independently is NR1R2, wherein R1 and R2 are independently selected from the group consisting of H and C1-C6 alkyl, or R1 and R2 combine to form together with the nitrogen to which they are attached a heterocyclic group with 4 to 5 carbon atoms, is used to denote a single or a double bond, and Z is NH, NH2, NH—(C═O)—(CH2)n—SH, wherein n=1 to 10, or or a tautomer or stereoisomer thereof, or a salt thereof. A method and device for detecting an analyte using Surface Enhanced Raman Spectroscopy (SERS) is also provided.
摘要翻译：提供了使用表面增强拉曼光谱（SERS）检测分析物的化合物和形成化合物的方法。化合物具有式I：其中W选自任选取代的芳基和任选取代的杂芳基; 每个Y独立地是NR 1 R 2，其中R 1和R 2独立地选自H和C 1 -C 6烷基，或者R 1和R 2与它们所连接的氮一起形成，所述氮与具有4至5个碳原子的杂环基，用于表示单键或双键，Z是NH，NH 2，NH-（C = O） - （CH 2）n-SH，其中n = 1-10，或其互变异构体或立体异构体，或其盐。还提供了使用表面增强拉曼光谱（SERS）检测分析物的方法和装置。

86. 发明申请

US20150080232A1 DNA SEQUENCING BY SYNTHESIS USING RAMAN AND INFRARED SPECTROSCOPY DETECTION 有权
标题翻译：通过使用拉曼和红外光谱检测合成的DNA序列
公开(公告)号：US20150080232A1
公开(公告)日：2015-03-19
申请号：US14119846
申请日：2012-05-23
申请人： Jingyue Ju , Jian Wu , Zengmin Li
发明人： Jingyue Ju , Jian Wu , Zengmin Li
IPC分类号： C12Q1/68
CPC分类号： C07H19/06 , C07H19/16 , C07H21/00 , C12Q1/6827 , C12Q1/6869 , C12Q1/6874 , C12Q2525/101 , C12Q2533/101 , C12Q2565/632
摘要： This invention provides nucleoside triphosphate analogues having the structure: wherein B is a base and is adenine, guanine, cytosine, uracil or thymine, wherein R″ is an OH or an H, and wherein R′ is azidomethyl, a hydrocarbyl, or a substituted hydrocarbyl, and which has a Raman spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1 or a Fourier transform-infrared spectroscopy spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1, and also to methods of DNA sequencing and SNP detection.
摘要翻译：本发明提供了具有以下结构的核苷三磷酸类似物：其中B为碱基，为腺嘌呤，鸟嘌呤，胞嘧啶，尿嘧啶或胸腺嘧啶，其中R“为OH或H，并且其中R'为叠氮基甲基，烃基或取代烃基，并且具有波长为2000cm -1至2300cm -1的拉曼光谱峰或波数为2000cm -1至2300cm -1的傅立叶变换红外光谱光谱峰，以及DNA测序方法和SNP检测。

87. 发明申请

US20140234940A1 MUTANT RB69 DNA POLYMERASE 有权
标题翻译： MUTANT RB69 DNA聚合酶
公开(公告)号：US20140234940A1
公开(公告)日：2014-08-21
申请号：US14191997
申请日：2014-02-27
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Marian PERIS , Michael PHELAN , Barnett ROSENBLUM , Stephen HENDRICKS
IPC分类号： C12N9/12
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
摘要翻译：本文提供了衍生自野生型RB69 DNA聚合酶或与野生型RB69 DNA聚合酶相关的突变型DNA依赖性聚合酶。这些突变型聚合酶能够选择性地结合标记的核苷酸。这些突变型聚合酶还能够掺入多种天然存在和修饰的核苷酸，包括例如终止子核苷酸。

88. 发明申请

US20130029360A1 DIMERIC CORE-SHELL NANOSTRUCTURE LABELED WITH RAMAN ACTIVE MOLECULE LOCALIZED AT INTERPARTICLE JUNCTION, USE THEREOF, AND METHOD FOR PREPARING THE SAME 审中-公开
标题翻译：用拉曼激活分子标记的二核心体纳米结构，其使用方法及其制备方法
公开(公告)号：US20130029360A1
公开(公告)日：2013-01-31
申请号：US13514920
申请日：2010-12-10
申请人： Yung Doug Suh , Jwa Min Nam , Dong Kwon Lim , Ki Seok Jeon
发明人： Yung Doug Suh , Jwa Min Nam , Dong Kwon Lim , Ki Seok Jeon
IPC分类号： G01N33/566 , C07K16/00 , C07H21/00 , C12N9/96 , C07K14/00 , B82Y15/00
CPC分类号： G01N33/54373 , C12Q1/6816 , C12Q1/6834 , C12Q2563/107 , C12Q2563/155 , C12Q2565/628 , G01N33/54346 , C12Q2565/632 , C12Q2563/143 , C12Q2537/125
摘要： The present invention relates to a nanoparticle dimer in which Raman-active molecules are located at a binding portion of the nanoparticle dimer, and more particularly, to a core-shell nanoparticle dimer comprising: a gold or silver core having a surface to which oligonucleotides are bonded; and a gold or silver shell covering the core. In addition, the present invention relates to the core-shell nanoparticle dimer, to a method for preparing same, and to the use thereof.
摘要翻译：本发明涉及纳米颗粒二聚体，其中拉曼活性分子位于纳米颗粒二聚体的结合部分，更具体地涉及核 - 壳纳米颗粒二聚体，其包含：具有寡核苷酸表面的金或银芯保税以及覆盖芯的金或银壳。此外，本发明涉及核 - 壳纳米颗粒二聚体，其制备方法及其用途。

89. 发明申请

US20110294691A1 ENHANCED ON-CHIP SERS BASED BIOMOLECULAR DETECTION USING ELECTROKINETICALLY ACTIVE MICROWELLS 有权
标题翻译：使用电动活性微球增强基于片上SERS的生物分子检测
公开(公告)号：US20110294691A1
公开(公告)日：2011-12-01
申请号：US13124296
申请日：2009-10-14
申请人： David Erickson , Yun Suk Huh , Carl A. Batt , Adam Joseph Lowe
发明人： David Erickson , Yun Suk Huh , Carl A. Batt , Adam Joseph Lowe
IPC分类号： C40B30/04 , C12M1/34 , C07H1/00 , C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6827 , B01F13/0076 , B01L3/50273 , B01L3/502761 , B01L2300/0636 , B01L2300/0654 , B01L2300/0809 , B01L2300/0829 , B01L2300/0887 , B01L2400/0415 , B01L2400/0424 , C12Q1/6862 , C12Q2565/632 , G01N21/658 , C12Q2563/137 , C12Q2531/137
摘要： A method for detecting target nucleic acids such as SNPs is provided. The method comprises performing a ligase detection reaction (LDR), performing surface enhanced Raman scattering (SERS) on the LDR, and analyzing the outcome of the LDR using analysis and/or quantification of the SERS by detecting an emitted Raman signature. The LDR-SERS method can be used for sensitive and specific detection of any nucleic acid sequence of interest. A microfluidic SERS detection device is also provided. The device comprises electrokinetically active microwells for mixing and concentrating analytes and in which analytes can be quantified. The device can be used for performing the LDR-SERS method in optofluidic chip format.
摘要翻译：提供了检测目标核酸如SNP的方法。该方法包括在LDR上进行连接酶检测反应（LDR），进行表面增强拉曼散射（SERS），并通过检测发射的拉曼特征来分析和/或定量SERS来分析LDR的结果。 LDR-SERS方法可用于感兴趣的任何核酸序列的敏感和特异性检测。还提供了微流体SERS检测装置。该装置包括用于混合和浓缩分析物的电动活性微孔，并且其中可分析物被量化。该器件可用于以光流体芯片格式执行LDR-SERS方法。

90. 发明授权

US07985539B2 Nanoparticle probes with raman spectroscopic fingerprints for analyte detection 有权
标题翻译：具有拉曼光谱指纹的纳米粒子探针用于分析物检测
公开(公告)号：US07985539B2
公开(公告)日：2011-07-26
申请号：US10431341
申请日：2003-05-07
申请人： Chad A. Mirkin , Yunwei Cao , Rongchao Jin
发明人： Chad A. Mirkin , Yunwei Cao , Rongchao Jin
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： G01N33/587 , C12Q1/6816 , G01N33/54373 , G01N33/583 , Y02A50/54 , C12Q2565/632 , C12Q2565/519 , C12Q2537/125
摘要： The invention encompasses reagents comprising particles with at least one Raman dye and a specific binding members bound thereto and methods of using such reagents. The invention also encompasses reagents of a specific binding member and two or more different Raman dyes and methods for using such reagents. New types of particle probes having a specific binding member bound thereto are described. These reagents are used in a novel detection strategy that utilizes the catalytic properties of the Au nanoparticles to generate a silver coating that can behave as a surface-enhanced Raman scattering (SERS) promoter for the dye-labeled particles that have been captured by target and an underlying chip in microarray format. The strategy provides the high sensitivity and high selectivity attributes of grey-scale scanometric detection but provides a route to multiplexing and ratioing capabilities since a very large number of probes can be designed based upon the concept of using a Raman tag as a spectroscopic fingerprint in detection. These spectra are used as fingerprints to differentiate oligonucleotide or other targets in one solution. This method has been used to distinguish six dissimilar DNA targets with six Raman labeled nanoparticle probes, and also two RNA targets with single nucleotide polymorphisms (SNPs).
摘要翻译：本发明包括含有至少一种拉曼染料和与其结合的特异性结合成员的颗粒的试剂和使用这些试剂的方法。本发明还包括特异性结合成员和两种或更多种不同拉曼染料的试剂和使用这些试剂的方法。描述具有与其结合的特异性结合成员的新型粒子探针。这些试剂用于一种新的检测策略，其利用Au纳米粒子的催化性能产生可以作为被目标捕获的染料标记的颗粒的表面增强拉曼散射（SERS）启动子的银涂层，微芯片格式的底层芯片。该策略提供了灰度扫描测量检测的高灵敏度和高选择性属性，但是提供了多路复用和比较能力的途径，因为可以基于在检测中使用拉曼标签作为光谱指纹的概念来设计非常大量的探针。这些光谱用作指纹以在一种溶液中区分寡核苷酸或其他靶标。该方法已被用于区分六个不同的DNA靶标与六个拉曼标记的纳米颗粒探针，以及两个具有单核苷酸多态性（SNPs）的RNA靶标。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式