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81. 发明申请

US20070259338A1 PARTICLE BASED HOMOGENEOUS ASSAYS USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION 有权
标题翻译：使用具有激光诱导荧光检测的毛细管电泳的基于粒子的均质测定
公开(公告)号：US20070259338A1
公开(公告)日：2007-11-08
申请号：US11611072
申请日：2006-12-14
申请人： Anthony Cheng , Julie Kim , Chan Oh
发明人： Anthony Cheng , Julie Kim , Chan Oh
IPC分类号： C12Q1/70 , C12Q1/68 , G01N33/574 , G01N33/553
CPC分类号： G01N27/44721 , C12Q1/6818 , G01N21/6402 , G01N33/542 , G01N33/54346 , C12Q2565/101 , C12Q2545/107
摘要： The invention provides highly sensitive and rapid homogeneous assays which employ particle-enhanced assay formats in concert with capillary electrophoresis and laser-induced fluorescence (LIF) detection to determine the concentration of an analyte of interest in a sample. Such a determination is made by measuring fluorescent signal(s) (i.e., an electropherogram) produced upon LIF of species present in the reaction mixture that are capable of producing such signals. The method of this invention produces simplified electropherograms by reducing the number of signals that must be separated and subsequently measured, and therefore increases the accuracy of the detection and/or quantification of target analyte concentration in a sample.
摘要翻译：本发明提供了高灵敏度和快速的均相测定法，其采用与毛细管电泳和激光诱导荧光（LIF）检测相结合的粒子增强测定形式，以确定样品中感兴趣的分析物的浓度。通过测量在能够产生这种信号的反应混合物中存在的LIF上产生的荧光信号（即，电泳图）来进行这种测定。本发明的方法通过减少必须分离和随后测量的信号的数量来产生简化的电泳图，因此增加了样品中目标分析物浓度的检测和/或定量的准确性。

82. 发明申请

US20070117137A1 METHOD FOR THE DETERMINATION OF A NUCLEIC ACID USING A CONTROL 有权
标题翻译：使用对照测定核酸的方法
公开(公告)号：US20070117137A1
公开(公告)日：2007-05-24
申请号：US11613996
申请日：2006-12-20
申请人： Stephan Jaeger
发明人： Stephan Jaeger
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6851 , C12Q2545/114 , C12Q2545/101 , C12Q2525/301 , C12Q2545/107
摘要： The present invention is directed to a method for the determination of a target nucleic acid using a special control nucleic acid, a method for the amplification of a partial sequence of said target nucleic acid using primers, a special control and a kit containing said control. The sequence of these control nucleic acids are at least in part parallel-complementary to the sequence of the target nucleic. These controls have similar properties as the target nucleic acid in hybridization and amplification methods, but can be well differentiated from the target nucleic acid by their different sequence.
摘要翻译：本发明涉及使用特异性对照核酸测定靶核酸的方法，使用引物扩增所述靶核酸的部分序列的方法，特殊对照和含有所述对照的试剂盒。这些对照核酸的序列至少部分与靶核酸序列平行互补。这些对照在杂交和扩增方法中具有与靶核酸相似的性质，但可以通过它们的不同序列与靶核酸进行良好的分化。

83. 发明申请

US20060228703A1 Displacement assay for the detection of nucleic acid oligomer hybridization events 审中-公开
标题翻译：用于检测核酸低聚物杂交事件的位移测定
公开(公告)号：US20060228703A1
公开(公告)日：2006-10-12
申请号：US10487816
申请日：2002-08-26
申请人： Gerhard Hartwich , Peter Frischmann , Ute Haker , Herbert Wieder
发明人： Gerhard Hartwich , Peter Frischmann , Ute Haker , Herbert Wieder
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q2545/107
摘要： Described is a method for detection of nucleic acid oligomer hybridization events, the method comprising the steps: providing a modified surface, the modification consisting in the attachment of at least one type of ligate nucleic acid oligomer; providing signal nucleic acid oligomer ligands; providing a sample having nucleic acid oligomer ligands; bringing a defined quantity of the signal nucleic acid oligomer ligands into contact with the modified surface and bringing the sample and the nucleic acid oligmer ligands contained therein into contact with the modified surface; detecting the signal nucleic acid oligomer ligands; and comparing with reference values the values obtained from the detection of the signal nucleic acid oligomer ligands.
摘要翻译：描述了用于检测核酸寡聚体杂交事件的方法，所述方法包括以下步骤：提供修饰的表面，所述修饰组合在至少一种类型的连接核酸寡聚体的连接中; 提供信号核酸寡聚体配体; 提供具有核酸低聚物配体的样品; 使定义量的信号核酸低聚物配体与修饰表面接触并使其中含有的样品和核酸寡聚体配体与改性表面接触; 检测信号核酸低聚物配体; 并且与参考值比较，从检测信号核酸低聚物配体获得的值。

84. 发明授权

US07029843B1 Method for the quantitative detection of nucleic acids 失效
标题翻译：核酸定量检测方法
公开(公告)号：US07029843B1
公开(公告)日：2006-04-18
申请号：US09831820
申请日：1999-11-17
申请人： Giuseppe Locatelli , Paolo Lusso , Mauro Malnati , Francesca Salvatori , Gabriella Scarlatti
发明人： Giuseppe Locatelli , Paolo Lusso , Mauro Malnati , Francesca Salvatori , Gabriella Scarlatti
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02
CPC分类号： C12Q1/6851 , C12Q1/6818 , C12Q1/6823 , C12Q1/703 , C12Q1/705 , C12Q2561/101 , C12Q2545/101 , C12Q2565/1015 , C12Q2521/319 , C12Q2545/107
摘要： Provided herein is a method for the quantitative detection of nucleic acids based on the use of a calibrator, suitable primers and probes, and a nucleic acid polymerase with 5′-3′ nuclease activity.
摘要翻译：本文提供了基于使用校准品，合适的引物和探针以及具有5'-3'核酸酶活性的核酸聚合酶定量检测核酸的方法。

85. 发明申请

US20060014200A1 Apparatus for analysis of a nucleic acid amplification reaction 审中-公开
公开(公告)号：US20060014200A1
公开(公告)日：2006-01-19
申请号：US11225247
申请日：2005-09-12
申请人： William McMillan , Lee Christel , David Borkholder , Steven Young
发明人： William McMillan , Lee Christel , David Borkholder , Steven Young
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6851 , B01L7/52 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/645 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6471 , G01N2035/00366 , G01N2035/00376 , G01N2035/0097 , G01N2201/062 , G01N2201/0627 , C12Q2537/165 , C12Q2545/107 , C12Q2545/113 , C12Q2545/101
摘要： An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. A controller in communication with the detection mechanism is programmed to store signal values defining a growth curve for the nucleic acid sequence, determine a derivative of the growth curve, and calculate a cycle number or time value associated with a characteristic of the derivative.

86. 发明申请

US20050260665A1 Single label comparative hybridization 有权
标题翻译：单标记比较杂交
公开(公告)号：US20050260665A1
公开(公告)日：2005-11-24
申请号：US11134133
申请日：2005-05-19
申请人： Mansoor Mohammed , Natasa Dzidic , Christopher McCaskill , Jaeweon Kim
发明人： Mansoor Mohammed , Natasa Dzidic , Christopher McCaskill , Jaeweon Kim
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6823 , C12Q1/6825 , C12Q1/6841 , C12Q1/6883 , C12Q2600/16 , C12Q2565/501 , C12Q2539/101 , C12Q2537/155 , C12Q2545/107
摘要： The present invention provides methods of detecting and mapping chromosomal or genetic abnormalities associated with various diseases or with predisposition to various diseases, or to detecting the phenomena of large scale copy number variants. In particular, the present invention provides advanced methods of performing array-based comparative hybridization that allow reproducibility between samples and enhanced sensitivity by using the same detectable label for both test sample and reference sample nucleic acids. Invention methods are useful for the detection or diagnosis of particular disease conditions such as cancer, and detecting predisposition to cancer based on detection of chromosomal or genetic abnormalities and gene expression level. Invention methods are also useful for the detection or diagnosis of hereditary genetic disorders or predisposition thereto, especially in prenatal samples. Moreover, invention methods are also useful for the detection or diagnosis of de novo genetic aberrations associated with post-natal developmental abnormalities.
摘要翻译：本发明提供了检测和映射与各种疾病相关的染色体或遗传异常或具有各种疾病倾向的方法，或者检测大规模拷贝数变体的现象。特别地，本发明提供了进行基于阵列的比较杂交的先进方法，其通过使用相同的可检测标记用于测试样品和参照样品核酸，从而允许样品之间的再现性和增强的灵敏度。本发明方法可用于检测或诊断特定的疾病状况如癌症，并且基于染色体或遗传异常和基因表达水平的检测来检测癌症易感性。本发明的方法也可用于遗传性遗传疾病或其易感性的检测或诊断，特别是在产前样品中。此外，发明方法也可用于检测或诊断与产后发育异常相关的从头遗传畸变。

87. 发明申请

US20050239128A1 Method for the quantitative detection of nucleic acids 失效
标题翻译：核酸定量检测方法
公开(公告)号：US20050239128A1
公开(公告)日：2005-10-27
申请号：US11169694
申请日：2005-06-30
申请人： Giuseppe Locatelli , Paolo Lusso , Mauro Malnati , Francesca Salvatori , Gabriella Scarlatti
发明人： Giuseppe Locatelli , Paolo Lusso , Mauro Malnati , Francesca Salvatori , Gabriella Scarlatti
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6851 , C12Q1/6818 , C12Q1/6823 , C12Q1/703 , C12Q1/705 , C12Q2561/101 , C12Q2545/101 , C12Q2565/1015 , C12Q2521/319 , C12Q2545/107
摘要： Provided herein is a method for the quantitative detection of nucleic acids based on the use of a calibrator, suitable primers and probes, and a nucleic acid polymerase with 5′-3′ nuclease activity.
摘要翻译：本文提供了基于使用校准品，合适的引物和探针以及具有5'-3'核酸酶活性的核酸聚合酶定量检测核酸的方法。

88. 发明申请

US20040115618A1 Method of quantification hiv-1rna-dna hybrid and diagnosis kit 审中-公开
标题翻译：定量方法hiv-1rna-dna杂交和诊断试剂盒
公开(公告)号：US20040115618A1
公开(公告)日：2004-06-17
申请号：US10416530
申请日：2003-05-27
发明人： Shingo Kato
IPC分类号： C12Q001/70 , C12Q001/68 , C12P019/34
CPC分类号： C12Q1/703 , C12Q1/6851 , C12Q2549/119 , C12Q2545/107 , C12Q2521/301
摘要： A diagnostic kit for evaluating the progress of an HIV-1-related disease and/or the efficacy of an anti-HIV-1 treatment using the quantity of an HIV-1 RNA-DNA hybrid in a sample as an indicator, comprising: at least one primer pair consisting of a downstream primer having a sequence complementary to a portion of the nucleotide sequence of the constituent RNA of the HIV-1 RNA-DNA hybrid and an upstream primer having a sequence complementary to a portion of the nucleotide sequence of the constituent DNA of the HIV-1 RNA-DNA hybrid; and restriction enzyme by which double-stranded DNA containing the same nucleotide sequence as DNA extended by the primer pair can be cleaved at any specific site in the nucleotide sequence.
摘要翻译：一种用于评估HIV-1相关疾病进展和/或使用样品中的HIV-1 RNA-DNA杂交体的量作为指标的抗HIV-1治疗的功效的诊断试剂盒，其包括：由具有与HIV-1 RNA-DNA杂交体的构成RNA的核苷酸序列的一部分互补的序列的下游引物组成的至少一个引物对和具有与所述HIV-1 RNA-DNA杂交体的核苷酸序列的一部分互补的序列的上游引物 HIV-1 RNA-DNA杂交体的构成DNA; 以及限制酶，通过该限制酶，可以在核苷酸序列的任何特定位点切割含有与引物对延伸的DNA相同的核苷酸序列的双链DNA。

89. 发明申请

US20030148278A1 Test kit and method for quantitatively detecting genetically modified DNA in foodstuff by means of fluorescence-coupled PCR 审中-公开
标题翻译：通过荧光偶联PCR定量检测食品中遗传修饰的DNA的试剂盒和方法
公开(公告)号：US20030148278A1
公开(公告)日：2003-08-07
申请号：US09922449
申请日：2001-08-03
申请人： Bioinside GmbH
发明人： Frank-Roman Lauter , Lutz Grohmann , Roger Petersen
IPC分类号： C12Q001/68 , C12P019/34
CPC分类号： C12Q1/6895 , C12Q1/686 , C12Q2561/101 , C12Q2545/113 , C12Q2545/107
摘要： The invention relates to a test kit and a specific method for qualitatively and quantitatively detecting DNA in foodstuff. The invention also relates to foodstuff products which come from genetically modified organisms (GMO), preferably from the RRS gene and the Bt-176 maize gene.
摘要翻译：本发明涉及一种用于定性和定量检测食品中DNA的测试试剂盒和具体方法。本发明还涉及来自转基因生物（GMO）的食品，优选来自RRS基因和Bt-176玉米基因。

90. 发明申请

US20030054372A1 Method for the determination of a nucleic acid using a control 有权
标题翻译：使用对照测定核酸的方法
公开(公告)号：US20030054372A1
公开(公告)日：2003-03-20
申请号：US10087631
申请日：2002-03-01
发明人： Stephan Jaeger
IPC分类号： C12Q001/68 , C12P019/34
CPC分类号： C12Q1/686 , C12Q1/6851 , C12Q2545/114 , C12Q2545/101 , C12Q2525/301 , C12Q2545/107
摘要： The present invention is directed to a method for the determination of a target nucleic acid using a special control nucleic acid, a method for the amplification of a partial sequence of said target nucleic acid using primers, a special control and a kit containing said control. The sequence of these control nucleic acids are at least in part parallel-complementary to the sequence of the target nucleic. These controls have similar properties as the target nucleic acid in hybridization and amplification methods, but can be well differentiated from the target nucleic acid by their different sequence.
摘要翻译：本发明涉及使用特异性对照核酸测定靶核酸的方法，使用引物扩增所述靶核酸的部分序列的方法，特殊对照和含有所述对照的试剂盒。这些对照核酸的序列至少部分与靶核酸序列平行互补。这些对照在杂交和扩增方法中具有与靶核酸相似的性质，但可以通过它们的不同序列与靶核酸进行良好的分化。

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