专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

81. 发明申请

US20110277043A1 Desaturases and Process for the Production of Polyunsaturated Fatty Acids in Transgenic Organisms 有权
标题翻译：去饱和酶和转基因生物中多不饱和脂肪酸生产的方法
公开(公告)号：US20110277043A1
公开(公告)日：2011-11-10
申请号：US13139014
申请日：2009-12-08
申请人： Toralf Senger , Jörg Bauer , Johnathan A. Napier
发明人： Toralf Senger , Jörg Bauer , Johnathan A. Napier
IPC分类号： C12N9/02 , C12N15/63 , C07K16/40 , A01K67/033 , A01H5/00 , C12N15/85 , C12P7/64 , C12N15/82 , C12N5/10 , C12N1/15 , C12N1/19 , C12N1/21 , A23D9/00 , A23D7/00 , A23L1/30 , A23K1/00 , A23K1/18 , C12N15/53
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472 , C12P7/6481 , C12Y114/19001
摘要： The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerina and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.
摘要翻译：本发明涉及来自嗜球菌（Cocchioiobolus heterostrophus）C5，蓝藻（Cyanothece sp。）的多核苷酸 CCY0110，密科霉菌和寄生于真菌的孢子菌，其编码去饱和酶，可用于重组生产多不饱和脂肪酸。本发明还涉及包含根据本发明的多核苷酸的载体，宿主细胞和转基因非人生物以及由多核苷酸编码的多肽。本发明还涉及针对根据本发明的多肽的抗体。最后，本发明还涉及多不饱和脂肪酸和油，脂和脂肪酸组合物的生产方法以及它们作为药物，化妆品，食品，饲料，优选鱼食或食品添加剂的用途。

82. 发明授权

US08048653B2 Microbial oils produced in transformed host cells comprising delta-9 elongases 有权
标题翻译：在包含δ-9延伸酶的转化宿主细胞中产生的微生物油
公开(公告)号：US08048653B2
公开(公告)日：2011-11-01
申请号：US12619706
申请日：2009-11-17
申请人： Howard Glenn Damude , Brian McGonigle , Zhixiong Xue , Quinn Qun Zhu
发明人： Howard Glenn Damude , Brian McGonigle , Zhixiong Xue , Quinn Qun Zhu
IPC分类号： C12P7/64 , C12N9/00 , C12N9/10 , C12N1/20 , C12N15/00
CPC分类号： C12N15/8247 , C11B1/025 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in plants and oleaginous yeast are disclosed.
摘要翻译：本发明涉及具有将亚油酸[18：2，LA]转化成二十碳烯酸[20：2，EDA]的能力的“Dgr”9延伸酶。公开了分离的核酸片段和重组构建体，其包含编码'Dgr 9延长酶的这种片段以及使用这些植物和油性酵母中的这些Dgr 9延伸酶制备长链多不饱和脂肪酸（PUFA）的方法。

83. 发明申请

US20110262975A1 ENGINEERED CO2 FIXING MICROORGANISMS PRODUCING CARBON-BASED PRODUCTS OF INTEREST 有权
标题翻译：工程化二氧化碳固定微生物生产基于碳的产品
公开(公告)号：US20110262975A1
公开(公告)日：2011-10-27
申请号：US13174580
申请日：2011-06-30
申请人： David A. Berry , Dan E. Robertson , Frank A. Skraly , Brian D. Green , Christian P. Ridley , Sriram Kosuri , Nikos B. Reppas , Martha Sholl , Noubar B. Afeyan
发明人： David A. Berry , Dan E. Robertson , Frank A. Skraly , Brian D. Green , Christian P. Ridley , Sriram Kosuri , Nikos B. Reppas , Martha Sholl , Noubar B. Afeyan
IPC分类号： C12P19/12 , C12P7/02
CPC分类号： C12P7/065 , C12N1/20 , C12N9/00 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0083 , C12N9/1007 , C12N9/1029 , C12N9/88 , C12N15/74 , C12P1/04 , C12P5/00 , C12P5/02 , C12P5/026 , C12P7/00 , C12P7/6409 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12P7/649 , C12P13/04 , C12R1/01 , C12Y103/03006 , Y02E50/13 , Y02E50/17 , Y02E50/18 , Y02E50/343
摘要： The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.
摘要翻译：本发明公开了确定感兴趣的碳基产物如乙醇，乙烯，化学品，聚合物，正烷烃，类异戊二烯，药物产品或其中间体在光自养生物体中生产的途径和机制，使得这些生物有效地转化二氧化碳和特别是利用这些有机物用于商业生产乙醇，乙烯，化学品，聚合物，正烷烃，类异戊二烯，药物产品或其中间体。

84. 发明授权

US08008050B2 Methods for discouraging bacterial growth in culture medium 有权
标题翻译：抑制培养基细菌生长的方法
公开(公告)号：US08008050B2
公开(公告)日：2011-08-30
申请号：US11738422
申请日：2007-04-20
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
IPC分类号： C12P7/64 , C12N1/12 , C11B1/00 , A23D9/00 , A23L1/30
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译：公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。

85. 发明授权

US07981641B2 Processes for the production of triglycerides of unsaturated fatty acids in the presence of enzymes 有权
标题翻译：在酶存在下生产不饱和脂肪酸甘油三酸酯的方法
公开(公告)号：US07981641B2
公开(公告)日：2011-07-19
申请号：US12061005
申请日：2008-04-02
申请人： Ulrich Schoerken , Sabine Both , Carolin Meyer , Peter Horlacher
发明人： Ulrich Schoerken , Sabine Both , Carolin Meyer , Peter Horlacher
IPC分类号： C12P7/64 , C08L91/00
CPC分类号： C12P7/6472
摘要： Processes for the enzyme-catalyzed production of triglycerides using polyunsaturated fatty acids, in which (a) the reaction of polyunsaturated fatty acids and/or C1-4 alkyl esters thereof with glycerol in vacuo in the presence of an immobilized enzyme to form their triglycerides is accelerated by addition of an additive from the group of weakly basic salts, complexing agents and ion exchangers and/or addition of a weakly basic salt and/or addition of an entraining agent in the form of a solvent or a gas and/or addition of glycerol-binding adsorbers and/or heat treatment of the partial glyceride intermediate product, (b) the immobilized enzymes are removed from the triglyceride by separation or filtration and (c) the remaining fatty acids and/or C1-4 alkyl esters thereof are removed from the triglyceride by distillation, refining or extraction.
摘要翻译：使用多不饱和脂肪酸进行酶催化生产甘油三酸酯的方法，其中（a）在固定化酶存在下，多不饱和脂肪酸和/或其中C 1-4烷基酯与甘油在真空中反应形成其甘油三酸酯的方法是通过加入来自弱碱性盐，络合剂和离子交换剂的添加剂和/或加入弱碱性盐和/或以溶剂或气体的形式加入夹带剂和/或添加甘油结合吸附剂和/或热处理部分甘油酯中间产物，（b）通过分离或过滤从甘油三酯中除去固定化的酶，和（c）除去剩余的脂肪酸和/或其中的C 1-4烷基酯从甘油三酯通过蒸馏，精制或提取。

86. 发明授权

US07939305B2 PUFA-PKS genes from Ulkenia 有权
标题翻译：来自Ulkenia的PUFA-PKS基因
公开(公告)号：US07939305B2
公开(公告)日：2011-05-10
申请号：US11547921
申请日：2005-04-08
申请人： Markus Luy , Matthias Rüsing , Thomas Kiy
发明人： Markus Luy , Matthias Rüsing , Thomas Kiy
IPC分类号： C12N9/00
CPC分类号： C12P7/6472 , C12N15/52
摘要： The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.
摘要翻译：本发明涉及编码针对聚酮化合物合酶（PKS）特异性序列的基因。由此合成的PKS的特征在于其产生PUFA（多不饱和脂肪酸）的酶能力。除了使用所述核苷酸序列产生重组和/或转基因生物外，本发明还涉及鉴定相应的DNA序列。

87. 发明授权

US07932077B2 High eicosapentaenoic acid producing strains of Yarrowia lipolytica 有权
标题翻译：高二十碳五烯酸生产的解脂耶氏酵母菌株
公开(公告)号：US07932077B2
公开(公告)日：2011-04-26
申请号：US11265761
申请日：2005-11-02
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
IPC分类号： C12N1/16 , C12N9/02 , C12N9/08 , C12P7/64 , C07H21/04
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.
摘要翻译：描述了能够在总油馏分中产生大于25％二十碳五烯酸（EPA，ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。这些菌株包括表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使EPA能够合成和高度积累。要求生产宿主细胞，以及在所述宿主细胞内生产EPA的方法。

88. 发明授权

US07897393B2 PUFA polyketide synthase systems and uses thereof 有权
标题翻译： PUFA聚酮化合物系统及其用途
公开(公告)号：US07897393B2
公开(公告)日：2011-03-01
申请号：US11781880
申请日：2007-07-23
申请人： Craig A. Weaver , Ross Zirkle , James G. Metz
发明人： Craig A. Weaver , Ross Zirkle , James G. Metz
IPC分类号： C12N15/82 , C12N5/12 , C12N1/20 , C07H21/04
CPC分类号： C12P7/6472 , C07K14/195 , C12N9/001 , C12N9/1029 , C12N15/52 , C12N15/8247 , C12P7/62 , C12P7/6427
摘要： Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.
摘要翻译：公开了来自细菌微生物Shewanella japonica和Shewanella olleyana的完全多不饱和脂肪酸（PUFA）聚酮化合物（PKS）系统及其生物活性片段和同系物。更具体地，本发明涉及编码这种PUFA PKS系统的核酸，其包含这种PUFA PKS系统的蛋白质和结构域到包含这种PUFA PKS系统的转基因生物（植物和微生物），以及制备和使用本文公开的PUFA PKS系统。本发明还涉及基因修饰的植物和微生物以及通过操作PUFA聚酮化合物合酶（PKS）系统来有效地产生富含多种不饱和脂肪酸（PUFA）以及其它生物活性分子的脂质的方法。

89. 发明授权

US07897391B2 PUFA polyketide synthase systems and uses thereof 有权
公开(公告)号：US07897391B2
公开(公告)日：2011-03-01
申请号：US11781867
申请日：2007-07-23
申请人： Craig A. Weaver , Ross Zirkle , James G. Metz
发明人： Craig A. Weaver , Ross Zirkle , James G. Metz
IPC分类号： C12N15/82 , C12N5/12 , C12N1/20 , C07H21/04
CPC分类号： C12P7/6472 , C07K14/195 , C12N9/001 , C12N9/1029 , C12N15/52 , C12N15/8247 , C12P7/62 , C12P7/6427
摘要： Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

90. 发明授权

US07893320B2 Method for producing multiple unsaturated fatty acids in plants 有权
标题翻译：在植物中生产多种不饱和脂肪酸的方法
公开(公告)号：US07893320B2
公开(公告)日：2011-02-22
申请号：US10511621
申请日：2003-04-25
申请人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
发明人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12P7/6427 , C12N9/0083 , C12N15/8247 , C12P7/6472 , C12P7/6481
摘要： The present invention relates to a method for the production of fatty acid esters which comprise unsaturated fatty acids with at least three double bonds, and to free unsaturated fatty acids with a content of at least 1% by weight based on the total fatty acids present in the plants, by expressing at least one nucleic acid sequence which encodes a polypeptide with Δ6-desaturase activity and at least one nucleic acid sequence which encodes a polypeptide with Δ6-elongase activity. Advantageously, these nucleic acid sequences can, if appropriate, be expressed in the transgenic plant together with a third nucleic acid sequence which encodes a polypeptide with Δ5-desaturase activity. The invention furthermore relates to the use of defined nucleic acid sequences which encode polypeptides with a Δ6-desaturase activity, Δ6-elongase activity or Δ5-desaturase activity selected from a group of nucleic acid sequences, and/or to the use of nucleic acid constructs comprising the abovementioned nucleic acid sequences.
摘要翻译：本发明涉及一种生产脂肪酸酯的方法，所述脂肪酸酯包含具有至少三个双键的不饱和脂肪酸，以及游离不饱和脂肪酸，所述不饱和脂肪酸的含量为至少1重量％通过表达编码具有＆Dgr.6-去饱和酶活性的多肽的至少一个核酸序列和至少一个编码具有＆Dgr.6-延伸酶活性的多肽的核酸序列的植物。有利的是，如果合适，这些核酸序列可以与编码具有＆Dgr。5-去饱和酶活性的多肽的第三核酸序列一起在转基因植物中表达。本发明进一步涉及定义的核酸序列的用途，所述核酸序列编码具有选自一组核酸序列的＆Dgr .6-去饱和酶活性和/或Dgr。5-去饱和酶活性的多肽，和/或使用包含上述核酸序列的核酸构建体。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式