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81. 发明授权

US07109020B2 Leguminous bacterium having potentiated nitrogen fixation ability 失效
标题翻译：豆科植物具有增强的固氮能力
公开(公告)号：US07109020B2
公开(公告)日：2006-09-19
申请号：US10483225
申请日：2002-07-09
申请人： Hidetoshi Okuyama , Takuji Owada , Nobutoshi Ichinose
发明人： Hidetoshi Okuyama , Takuji Owada , Nobutoshi Ichinose
IPC分类号： C12N1/20 , C12N1/21
CPC分类号： C12N9/0065 , C05F11/08 , Y02P60/218
摘要： The invention of this application provides root nodule bacteria transformed with the DNA fragment of SEQ ID No. 1 consisting a catalase gene and whose nitrogen-fixation ability is enhanced with a catalase produced from the catalase gene, a preparation for leguminous crops containing the root nodule bacteria as an active ingredient, and a method of cultivating leguminous crops which comprises contacting seeds of leguminous crops with these. These inventions enable the acceleration of growth of leguminous crops and multiplication of crop yield of beans. Further, the reduction of agricultural cost and the alleviation of environmental pollution are realized by the reduction of nitrogen fertilizers.
摘要翻译：本申请的发明提供了用由过氧化氢酶基因构成的SEQ ID No.1的DNA片段转化的根瘤细菌，并且其通过由过氧化氢酶基因产生的过氧化氢酶增强了固氮能力，含有根结节的豆科作物的制剂细菌作为活性成分，以及培养豆科作物的方法，其包括将豆科作物的种子与这些接触。这些发明能够加速豆科作物的生长和豆类作物产量的增加。此外，降低农业成本和减轻环境污染是通过减少氮肥来实现的。

82. 发明授权

US07087426B2 Materials and methods for the modification of plant lignin content 失效
公开(公告)号：US07087426B2
公开(公告)日：2006-08-08
申请号：US10174693
申请日：2002-06-18
申请人： Leonard N. Bloksberg , Ikka Havukkala
发明人： Leonard N. Bloksberg , Ikka Havukkala
IPC分类号： C12N15/00 , C12N15/09 , C12N5/04 , C12N5/10 , C07H21/04
CPC分类号： C12Y110/03001 , C12N9/0004 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0059 , C12N9/0061 , C12N9/0065 , C12N9/0071 , C12N9/0083 , C12N9/1007 , C12N9/1011 , C12N9/1051 , C12N9/2422 , C12N9/2445 , C12N9/86 , C12N9/88 , C12N9/93 , C12N15/8255 , C12Y110/03002 , C12Y114/13011 , C12Y201/01006 , C12Y302/01001 , C12Y302/01021
摘要： Novel isolated polynucleotides and polypeptides associated with the lignin biosynthetic pathway are provided, together with genetic constructs including such sequences. Methods for the modulation of lignin content, lignin structure and lignin composition in target organisms are also disclosed, the methods comprising incorporating one or more of the polynucleotides of the present invention into the genome of a target organism.

83. 发明授权

US07083968B2 Microorganism of serratia family, isolation method and the preparation method of lignin lyases using this 有权
标题翻译：沙雷氏菌家族的微生物，分离方法和木质素裂解酶的制备方法
公开(公告)号：US07083968B2
公开(公告)日：2006-08-01
申请号：US10481233
申请日：2001-07-31
申请人： Ho Yong Park , Ki Duk Kim , Dong Ha Shin
发明人： Ho Yong Park , Ki Duk Kim , Dong Ha Shin
IPC分类号： C12N1/12
CPC分类号： C12Y110/03002 , C12N1/20 , C12N9/0061 , C12N9/0065 , C12R1/43 , C12Y111/01013 , C12Y111/01014
摘要： The present invention relates to a novel microbe belonging to Serratia family, its isolation method and the preparation method using it. More particularly, the present invention relates to a microorganism of Serratia family producing laccase, lignin peroxidase and Mn-dependent-peroxidase which are the kinds of lignin lyases, and a method for separating the microbe from insect gut, as well as a method for preparing laccase, lignin peroxidase and Mn-dependent-peroxidase. The microorganism of the present invention can be used not only in the treatment of industrial sewage and harmful environmental substance but also in oil degradation, in biological bleaching of pulp since it can produce lignin lyases. More over, the microbe can also be used effectively in industry of preparing fuel, forage and other chemicals through lignin degradation.
摘要翻译：本发明涉及属于沙雷氏菌家族的新型微生物，其分离方法及其制备方法。更具体地说，本发明涉及一种生产漆酶的沙雷氏菌家族微生物，木素过氧化物酶和木质素过氧化物酶，它们是木质素裂解酶的种类，以及从昆虫肠中分离微生物的方法以及一种制备方法漆酶，木质素过氧化物酶和Mn依赖性过氧化物酶。本发明的微生物不仅可用于工业污水和有害环境物质的处理，而且可用于油类生物漂白中的油分解，因为它可以生产木质素裂解酶。此外，微生物也可以有效地用于通过木质素降解制备燃料，饲料和其他化学品的工业。

84. 发明授权

US07052837B2 Histoplasma capsulatum catalase sequences and their use in the detection of Histoplamsa capsulatum and histoplasmosis 有权
标题翻译：组织胞浆过氧化氢酶序列及其在检测组织胞浆菌和组织胞浆菌病中的应用
公开(公告)号：US07052837B2
公开(公告)日：2006-05-30
申请号：US10099352
申请日：2002-03-13
申请人： Clayton H. Johnson , J. Lyndal York , Joan E. McEwen
发明人： Clayton H. Johnson , J. Lyndal York , Joan E. McEwen
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12N9/0065 , C12Q1/6895 , C12Q2600/158 , G01N33/56961 , G01N33/573
摘要： The present invention describes Histoplasmosis capsulatum catalase A and catalase P nucleic acid and protein sequences as reagents for the detection of H. capsulatum infection. Specifically, the invention describes intron sequences from the H. capsulatum catalase A (CATA) and catalase P (CATP) genes which can be used for hybridization and PCR based detection of H. capsulatum infection. In another embodiment, assays for H. capsulatum catalase P or catalase A polypeptides are used as diagnostic tests for H. capsulatum infection and histoplasmosis, respectively. Also described is the differentiation of H. capsulatum from Blastomyces dermititidis based on a H. capsulatum catalase P PCR based assay.
摘要翻译：本发明描述了组织胞浆菌过氧化氢酶A和过氧化氢酶P核酸和蛋白质序列作为检测荚膜梭菌感染的试剂。具体地，本发明描述了可用于杂交和PCR检测荚膜梭菌感染的H. capsulatum过氧化氢酶A（CATA）和过氧化氢酶P（CATP）基因的内含子序列。在另一个实施方案中，分别用于H. capsulatum过氧化氢酶P或过氧化氢酶A多肽的测定作为荚膜梭菌感染和组织胞浆菌病的诊断试验。还描述了基于基于H. capsulatum过氧化氢酶基于PCR的测定法，来自真皮酵母菌的H. capsulatum的分化。

85. 发明授权

US07015022B2 Mammalian catalase-dependent oxidation processes and methods for stimulating oxidative activities 失效
公开(公告)号：US07015022B2
公开(公告)日：2006-03-21
申请号：US10165841
申请日：2002-06-07
申请人： Jeffrey D. Laskin , Anna Marie Vetrano , Diane Heck
发明人： Jeffrey D. Laskin , Anna Marie Vetrano , Diane Heck
IPC分类号： C12N9/08
CPC分类号： C12N9/0065 , C12Q1/30 , C12Y111/01006 , G01N2500/02
摘要： A catalase-dependent enzymatic oxidation process wherein a substrate to be oxidized is contacted with catalase in the absence of hydrogen peroxide is provided. Also provided are methods for using this process in a variety of biomedical, clinical and diagnostic applications as well as industrial processes. A method for stimulating the enzymatic oxidation process by treatment with ultraviolet light and uses for this method are also provided.

86. 发明申请

US20060051851A1 Novel selenocysteine-containing protein 审中-公开
标题翻译：新型含硒半胱氨酸的蛋白质
公开(公告)号：US20060051851A1
公开(公告)日：2006-03-09
申请号：US10489716
申请日：2002-09-12
申请人： Sara Kaminaka , Kazuyoshio Kaminaka , Masaki Hirashima , Hiroaki Maeda , Chikateru Nozaki , Kazuhiko Takahashi
发明人： Sara Kaminaka , Kazuyoshio Kaminaka , Masaki Hirashima , Hiroaki Maeda , Chikateru Nozaki , Kazuhiko Takahashi
IPC分类号： C12N9/02 , C07H21/04 , C12P21/06
CPC分类号： C12N9/0065 , C07K14/765 , C07K2319/00
摘要： A novel selenocysteine-containing protein is provided which is a substantial entity having an enzymatic activity, typically an activity to reduce peroxide phospholipids. There are provided a gene consisting of a coding sequence for a protein not containing selenocysteine in which the codon for selenocysteine is introduced at a desired position and a selenocysteine insertion sequence resided at the 3′-end of said coding sequence and a protein expressed therefrom, typically a selenocysteine-containing protein having an activity to reduce peroxide phospholipids which is prepared by introducing selenocysteine into albumin and as well as a gene encoding said protein. A novel anti-oxidative substance is provided which can be applied for prevention of worsening, prophylaxis or treatment of various diseases associated with oxidative stress.
摘要翻译：提供了一种新型含硒代半胱氨酸的蛋白质，其是具有酶活性的实质实体，通常是减少过氧化物磷脂的活性。提供了由不含硒代半胱氨酸的蛋白质的编码序列组成的基因，其中在期望的位置引入硒代半胱氨酸的密码子，并且存在位于所述编码序列的3'端的硒代半胱氨酸插入序列和由其表达的蛋白质，通常是含有硒代半胱氨酸的蛋白质，其具有通过将硒代半胱氨酸引入白蛋白而制备的过氧化物磷脂的活性，以及编码所述蛋白质的基因。提供了一种新的抗氧化物质，其可用于预防与氧化应激相关的各种疾病的恶化，预防或治疗。

87. 发明申请

US20060048306A1 Method of using rice-origin peroxidase 审中-公开
标题翻译：使用稻米过氧化物酶的方法
公开(公告)号：US20060048306A1
公开(公告)日：2006-03-09
申请号：US10530399
申请日：2003-09-24
申请人： Miyuki Morita , Kiyoshi Ito
发明人： Miyuki Morita , Kiyoshi Ito
IPC分类号： C11D3/00
CPC分类号： C12N9/0065 , C11D3/0021 , C11D3/38654 , C11D3/3947
摘要： The present invention provides a method of using a rice peroxidase, particularly rice hull peroxidase, having excellent reactivity at high temperatures and reactivity. Dyed substances (dyes, etc.) are decomposed (discolored/bleached) in a washing liquor by means of a rice peroxidase. More particularly, in the case of washing or rinsing a portion with another portion in the dyed fabric with the same washing liquor, the transfer of textile dyes from the dyed fabric to the other fabric is inhibited. Further, textile dyes in a solution or a dispersion solution are bleached. The invention allows excellent prevention of dye transfer in washing or rinsing and thus provides a method better than bleaching textile dyes in a solution or a dispersion solution, and further a composition for prevention of dye transfer and for bleaching.
摘要翻译：本发明提供了一种在高温和高反应性下使用具有优异反应性的水稻过氧化物酶，特别是稻壳过氧化物酶的方法。染料（染料等）通过米过氧化物酶在洗涤液中分解（变色/漂白）。更具体地，在用相同的洗涤液洗涤或漂洗染色织物中的另一部分的部分的情况下，织物染料从染色织物转移到另一织物被抑制。此外，将溶液或分散溶液中的纺织染料漂白。本发明允许在洗涤或漂洗中优异地防止染料转移，从而提供比在溶液或分散液中漂白纺织染料更好的方法，还有用于防止染料转移和漂白的组合物。

88. 发明授权

US06991824B2 Expression of human milk proteins in transgenic plants 有权
公开(公告)号：US06991824B2
公开(公告)日：2006-01-31
申请号：US10077381
申请日：2002-02-14
申请人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
发明人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
IPC分类号： A21D2/26
CPC分类号： G01N33/57492 , A23K10/30 , A23K20/147 , A23L7/198 , A23L7/20 , A23L33/105 , A23L33/19 , A23V2002/00 , B82Y30/00 , C07K14/415 , C07K14/485 , C07K14/65 , C07K14/79 , C07K14/8125 , C12N9/0065 , C12N9/2462 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8257 , G01N33/57484 , G01N2333/4725 , G01N2500/02 , A23V2300/21
摘要： The invention is directed to food and food additive compositions comprising one or more human milk proteins produced in the seeds of a transgenic plant and methods of making the same. The invention is further directed to improved infant formula comprising such food supplement composition.

89. 发明申请

US20060021084A1 Production of peroxidase from plant cell and callus cultures 有权
标题翻译：从植物细胞和愈伤组织培养物中生产过氧化物酶
公开(公告)号：US20060021084A1
公开(公告)日：2006-01-26
申请号：US11136240
申请日：2005-05-24
申请人： Tholath Abraham , Nisha Devaki , Thomson Kuruvilla , Jegan Joseph
发明人： Tholath Abraham , Nisha Devaki , Thomson Kuruvilla , Jegan Joseph
IPC分类号： A01H1/00 , C12N9/08 , C12N5/04 , C12N15/82
CPC分类号： C12N9/0065
摘要： A process for the production of peroxidase which comprises of establishing a plant cell culture producing cells from neem (Azardiracta indica) and nirgundi (Vitex negundo) wherein the peroxidase has higher enzymatic activity not reported earlier.
摘要翻译：一种生产过氧化物酶的方法，其包括建立从neem（Azardiracta indica）和nirgundi（Vitex negundo）产生细胞的植物细胞培养物，其中过氧化物酶具有较高的酶活性，早先没有报道。

90. 发明申请

US20050164350A1 Heat-resistant thioredoxin and related enzymes 审中-公开
标题翻译：耐热硫氧还蛋白及相关酶
公开(公告)号：US20050164350A1
公开(公告)日：2005-07-28
申请号：US11084394
申请日：2005-03-18
申请人： Kazuhiko Ishikawa , Sung-jong Jeon , Yasuhiro Kashima
发明人： Kazuhiko Ishikawa , Sung-jong Jeon , Yasuhiro Kashima
IPC分类号： C12N9/02 , C12N9/08 , C07H21/04 , C12N1/21 , C12N15/74
CPC分类号： C12N9/0036 , C12N9/0065
摘要： The inventors isolated from hyperthermophilic archaebacteria Aeropyrum pernix a heat resistant thioredoxin, thioredoxin reductase and thioredoxin peroxidase having at least 50% residual activity after heat treatment at 100° C. for 0.5 hour, and sequenced the amino acid and base sequences. The inventors also isolated from hyperthermophilic archaebacteria Pyrococcus horikoshii a heat resistant thioredoxin, thioredoxin reductase, and thioredoxin peroxidase showing substantially no decline in activity when heat treated at 100° C. for 0.5 hour, and sequenced the amino acid and base sequences.
摘要翻译：本发明人从超嗜热古细菌艾氏青霉中分离出耐热硫氧还蛋白，硫氧还蛋白还原酶和硫氧还蛋白过氧化物酶，其在100℃热处理0.5小时后具有至少50％的残留活性，并对氨基酸和碱基序列进行测序。本发明人还从超嗜热古细菌Pyococcus horikoshii中分离出耐热硫氧还蛋白，硫氧还蛋白还原酶和硫氧还蛋白过氧化物酶，当在100℃热处理0.5小时时基本上没有活性下降，并测序氨基酸和碱基序列。

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