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    • 82. 发明申请
    • Method for controlling solute loading of polymer microparticles
    • 控制聚合物微粒溶质负载的方法
    • US20040142102A1
    • 2004-07-22
    • US10348165
    • 2003-01-21
    • BioArray Solutions, Ltd.
    • Sukanta BanerjeeCecilia GeorgescuMichael Seul
    • B05D007/00
    • C08J3/212C08J3/215C08J3/22C08J2325/08
    • Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.
    • 通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。 三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。 第二溶剂是用于溶质和聚合物(调谐溶剂)的弱溶剂或非溶剂。 第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂,但是作为相对于第一和第二溶剂的共溶剂,因为其可与第一溶剂和第二溶剂混溶。 通过调节溶质相对于微粒聚合物的比例,调节三元溶剂体系的组成,主要是调谐溶剂的量来控制掺入到微粒中的溶质的量。 该方法对于提供包含可区分的染料负载,特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。