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81. 发明授权

US07838631B2 Apparatus for measuring high density lipoproteins and method of separating high density lipoproteins 有权
标题翻译：用于测量高密度脂蛋白的装置和分离高密度脂蛋白的方法
公开(公告)号：US07838631B2
公开(公告)日：2010-11-23
申请号：US12131438
申请日：2008-06-02
申请人： Kotaro Yamashita , Masafumi Miyake
发明人： Kotaro Yamashita , Masafumi Miyake
IPC分类号： C07K14/775 , C07K1/30
CPC分类号： G01N33/92 , C07K1/30 , C07K1/34 , C07K14/775
摘要： The present invention provides a method of separating lipoproteins other than high density lipoproteins from a biological fluid. The method can quickly measure HDL cholesterol with a simple configuration and without the need of providing additional complicated devices. In this method, high density lipoproteins not generating any precipitate are fractionated from low density lipoproteins, very-low density lipoproteins, and chylomicrons generating precipitates. Then the precipitates are removed not by centrifugal separation based on the conventional technology, but by filtration using a filter to separate high density lipoproteins in blood serum. A hydrophilic cellulose-mixed ester is preferable as a material for the filter, and the pore diameter is 0.8 μm or below. When the filtering method is employed, it is possible to eliminate the complicated operations required in the conventional centrifugal separation, and to shorten the time it takes for separation of the high density lipoproteins.
摘要翻译：本发明提供了从生物体液中分离高密度脂蛋白以外的脂蛋白的方法。该方法可以以简单的配置快速测量HDL胆固醇，无需提供额外的复杂器件。在这种方法中，从低密度脂蛋白，极低密度脂蛋白和产生沉淀的乳糜微粒中分离不产生任何沉淀的高密度脂蛋白。然后基于常规技术，不是通过离心分离除去沉淀物，而是通过使用过滤器过滤以分离血清中的高密度脂蛋白。优选亲水性纤维素混合酯作为过滤材料，孔径为0.8μm以下。当采用过滤方法时，可以消除常规离心分离中所需的复杂操作，并缩短分离高密度脂蛋白所需的时间。

82. 发明授权

US07795010B2 Fractionator and method of fractionation 有权
$Fractionator and method of fractionation$
标题翻译：分馏器和分馏方法
公开(公告)号：US07795010B2
公开(公告)日：2010-09-14
申请号：US10586678
申请日：2005-01-20
申请人： Kazuhiro Tanahashi , Ichiro Kumo , Nobuyuki Kuroki , Hiroyuki Sugaya , Satoko Yamada , Shigehisa Wada , Giman Jung , Toshihiko Kuroda , Shuji Sekiguchi
发明人： Kazuhiro Tanahashi , Ichiro Kumo , Nobuyuki Kuroki , Hiroyuki Sugaya , Satoko Yamada , Shigehisa Wada , Giman Jung , Toshihiko Kuroda , Shuji Sekiguchi
IPC分类号： C12M3/00
CPC分类号： B01D63/04 , B01D61/142 , B01D61/20 , B01D63/08 , B01D2313/243 , B01D2319/022 , C07K1/22 , C07K1/34 , C07K1/36
摘要： The following is disclosed: (1) a membrane fractionator including a filtration section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; (2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system that in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human alpha1-microglobulin and human albumin of 1.5 to 1000, thereby separating part of the biocomponents; and (3) a method of protein fractionation, comprising bringing a solution containing two or more types of proteins and water into contact with a hollow yarn separation membrane to thereby attain protein fractionation, characterized in that the fractionation solution contains an organic solvent.
摘要翻译：以下公开了：（1）包括过滤部分，浓缩部分，回收部分和液体供给泵的膜分馏器，其中将过滤部分，浓缩部分和回收部分彼此连接的流动通道构成闭合电路 ; （2）一种生物成分分离方法，其特征在于，将来自生物成分的样品加入抗体吸附膜分离系统中，所述抗体吸附膜分离系统具有能够吸附在膜分离系统的中部或后部内部容纳的特定蛋白质的抗体，不存在吸附蛋白质的抗体，人α1-微球蛋白与人白蛋白之间的渗透比为1.5〜1000，从而分离部分生物成分; （3）蛋白质分级法的方法，其特征在于，使含有2种以上蛋白质和水的溶液与中空丝分离膜接触，从而进行蛋白质分馏，其特征在于，分离液含有有机溶剂。

83. 发明授权

US07759117B2 Use of depth filtration in series with continuous centrifugation to clarify mammalian cell cultures 有权
标题翻译：使用深度过滤串联连续离心澄清哺乳动物细胞培养
公开(公告)号：US07759117B2
公开(公告)日：2010-07-20
申请号：US11616772
申请日：2006-12-27
申请人： Christine Y. Pham
发明人： Christine Y. Pham
IPC分类号： C12S3/14
CPC分类号： C12N1/02 , C07K1/30 , C07K1/34 , C07K1/36 , C12M47/02 , Y10S435/803
摘要： Methods for clarification of cell samples using centrifugation in combination with depth filtration.
摘要翻译：使用离心与深度过滤相结合的细胞样品的澄清方法。

84. 发明申请

US20100145022A1 Method of Isolating Biomacromolecules Using Low pH and Divalent Cations 有权
标题翻译：使用低pH和二价阳离子隔离生物大分子的方法
公开(公告)号：US20100145022A1
公开(公告)日：2010-06-10
申请号：US12513137
申请日：2007-11-01
申请人： Jonathan Romero , James Chrostowski , Philippe Georges De Vilmorin , Jennifer Yun Case
发明人： Jonathan Romero , James Chrostowski , Philippe Georges De Vilmorin , Jennifer Yun Case
IPC分类号： C07K16/00 , C07K1/14
CPC分类号： C07K16/065 , C07K1/34
摘要： The present invention is related to a method of isolating a biological macromolecule in a composition. Specifically, the present invention is directed to a method of isolating a biomacromolecule in a composition containing an impurity, the method comprising (a) lowering the pH of the composition, (b) adding a divalent cation to the composition, and (c) separating the biomacromolecule from the impurity.
摘要翻译：本发明涉及在组合物中分离生物大分子的方法。具体地说，本发明涉及一种在含有杂质的组合物中分离生物大分子的方法，该方法包括：（a）降低组合物的pH，（b）向组合物中加入二价阳离子，和（c）分离来自杂质的生物大分子。

85. 发明申请

US20100041611A1 Rapid Isolation of Osteoinductive Protein Mixtures From Mammalian Bone Tissue 有权
标题翻译：从哺乳动物骨组织快速分离骨诱导蛋白混合物
公开(公告)号：US20100041611A1
公开(公告)日：2010-02-18
申请号：US12582476
申请日：2009-10-20
申请人： Kevin Thorne , Rama AKELLA
发明人： Kevin Thorne , Rama AKELLA
IPC分类号： A61K38/16 , A61P19/00
CPC分类号： C07K1/34 , A61K35/32 , A61K38/1875 , C07K1/36 , C07K14/51 , Y10S530/84
摘要： A method for purifying bone-derived osteoinductive proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recover process. The high and low ultrafiltration processes preferably select proteins having a nominal molecular weight between approximately 8 kilodaltons and approximately 100 kilodaltons. Processes of the present invention may be used to recover osteoinductive proteins from bone demineralization waste streams.
摘要翻译：一种用于纯化骨衍生的骨诱导蛋白的方法，包括脱矿质过程，蛋白质提取法，高分子量超滤法，低分子量超滤法和恢复法。高和低超滤方法优选选择标称分子量在约8千道尔顿和约100千道尔顿之间的蛋白质。本发明的方法可用于从骨脱矿质废物流中回收骨诱导蛋白。

86. 发明申请

US20090186395A1 Virus Removal by Nanofiltration 审中-公开
标题翻译：通过纳滤法去除病毒
公开(公告)号：US20090186395A1
公开(公告)日：2009-07-23
申请号：US12308850
申请日：2007-06-25
申请人： Israel Nur , Roni Mintz
发明人： Israel Nur , Roni Mintz
IPC分类号： C12N9/74 , C07K1/00
CPC分类号： C07K1/34 , A61L2/0017 , B01D61/027 , B01D2315/08 , B01D2315/10
摘要： A method for purifying a protein solution from infective particles comprising (a) adding a macromolecule to the solution; and (b) passing the solution through a nanofilter. The macromolecule may be selected from a polymer of at least 3 monomers of sugars, amino acids, glycols, alcohols, lipids or phospholipids.
摘要翻译：一种从感染性颗粒纯化蛋白质溶液的方法，包括（a）向溶液中加入大分子; 和（b）使溶液通过纳米过滤器。大分子可以选自糖，氨基酸，二醇，醇，脂质或磷脂中的至少3种单体的聚合物。

87. 发明申请

US20090179146A1 FLUIDICS DEVICE 有权
标题翻译：流体装置
公开(公告)号：US20090179146A1
公开(公告)日：2009-07-16
申请号：US11915151
申请日：2006-01-19
申请人： Lee O. Lomas , Jian Ding , Egisto Boschetti
发明人： Lee O. Lomas , Jian Ding , Egisto Boschetti
IPC分类号： B01D59/44 , B01D57/02 , B01D15/08 , C40B60/12
CPC分类号： G01N30/461 , B01D15/325 , B01D15/362 , B01D15/363 , B01L3/5025 , B01L3/50255 , B01L3/563 , B01L2200/026 , B01L2200/028 , B01L2300/0681 , B01L2300/0829 , B01L2300/087 , B01L2400/0487 , C07K1/34 , G01N27/44704 , G01N27/44795 , G01N30/466 , G01N30/468 , Y10T137/2224 , Y10T137/794 , Y10T137/86187 , Y10T436/255
摘要： The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.
摘要翻译：本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。

88. 发明申请

US20090166290A1 AUTOMATED LOW VOLUME CROSSFLOW FILTRATION 审中-公开
标题翻译：自动低容量横流过滤
公开(公告)号：US20090166290A1
公开(公告)日：2009-07-02
申请号：US12282893
申请日：2007-03-23
申请人： Lars Andersson
发明人： Lars Andersson
IPC分类号： B01D65/02 , B01D61/32 , G05B19/00 , G05D7/00
CPC分类号： C07K1/34 , B01D61/145 , G01N1/34 , G01N1/4005
摘要： The invention provides an automated crossflow filtration method and system for separating a component of interest from one or more other components in a solution. The invention is of particular use in the field of protein separations and concentration, where specific proteins must be separated and purified from cell lysates and cultures. The system may be under the control of a computer software programme.
摘要翻译：本发明提供了一种用于将感兴趣的组分与溶液中的一种或多种其它组分分离的自动交叉流过滤方法和系统。本发明在蛋白质分离和浓缩领域中特别有用，其中特异性蛋白质必须从细胞裂解物和培养物中分离和纯化。该系统可能在计算机软件程序的控制之下。

89. 发明授权

US07531632B2 Clarification of transgenic milk using depth filtration 有权
标题翻译：使用深度过滤澄清转基因牛奶
公开(公告)号：US07531632B2
公开(公告)日：2009-05-12
申请号：US11355557
申请日：2006-02-16
申请人： Mark A. Perreault
发明人： Mark A. Perreault
IPC分类号： C07K1/00
CPC分类号： C07K14/765 , C07K1/34
摘要： Processes and apparati are provided for separating molecules of interest from a mixture by depth filtration (DF). The DF of the invention is useful in the clarification and processing of various feedstreams for the removal of a molecule of interest. According to a preferred embodiment, a transgenic milk feedstream is stabilized and particulate matter such as fat, casein miscelles and bacteria are removed. An aseptic filtration step was also developed to remove any bacteria remaining in a clarified transgenic milk feedstream.
摘要翻译：提供了通过深度过滤（DF）从混合物中分离感兴趣的分子的方法和装置。本发明的DF可用于澄清和加工用于除去目的分子的各种原料流。根据一个优选的实施方案，转基因奶饲料流被稳定，除去脂肪，酪蛋白，细胞和细菌等颗粒物质。还开发了无菌过滤步骤以除去澄清的转基因乳饲料流中剩余的任何细菌。

90. 发明申请

US20090047681A1 ENTROPIC TRAPPING AND SIEVING OF MOLECULES 有权
标题翻译：分子诱导和分离
公开(公告)号：US20090047681A1
公开(公告)日：2009-02-19
申请号：US12209789
申请日：2008-09-12
申请人： Jongyoon Han , Harold G. Craighead
发明人： Jongyoon Han , Harold G. Craighead
IPC分类号： C12Q1/68
CPC分类号： C07K1/26 , B01D57/02 , B01L3/502761 , B01L2200/0668 , B01L2400/0421 , B01L2400/086 , C02F1/469 , C07K1/34 , G01N30/02 , G01N30/6082 , G01N30/6095 , G01N2030/285 , G01N2030/525 , B01D15/34 , G01N30/74
摘要： Nanofluidic entropic traps, comprising alternating thin and thick regions, sieve small molecules such as DNA or protein polymers and other molecules. The thick region is comparable or substantially larger than the molecule to be separated, while the thin region is substantially smaller than the size of the molecules to be separated. Due to the molecular size dependence of the entropic trapping effect, separation of molecules may be achieved. In addition, entropic traps are used to collect, trap and control many molecules in the nanofluidic channel. A fabrication method is disclosed to provide an efficient way to make nanofluidic constrictions in any fluidic devices.
摘要翻译：纳米流体熵陷阱，包括交替的薄和厚区域，筛小分子如DNA或蛋白质聚合物和其他分子。厚区域与待分离的分子相当或基本上大，而薄区域显着小于要分离的分子的大小。由于熵捕获效应的分子尺寸依赖性，可以实现分子的分离。此外，熵陷阱用于收集，捕获和控制纳米流体通道中的许多分子。公开了制造方法以提供在任何流体装置中制造纳米流体收缩的有效方式。

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