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71. 发明申请

US20080053205A1 Droplet-based particle sorting 有权
标题翻译：基于液滴的颗粒分选
公开(公告)号：US20080053205A1
公开(公告)日：2008-03-06
申请号：US11639566
申请日：2006-12-15
申请人： Michael Pollack , Vamsee Pamula , Vijay Srinivasan
发明人： Michael Pollack , Vamsee Pamula , Vijay Srinivasan
IPC分类号： G01N15/06 , B01J19/08
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
摘要翻译：本发明涉及基于液滴的颗粒分选。根据一个实施例，提供了液滴微致动器，其包括：（a）颗粒悬浮液; 和（b）布置成使用包含颗粒的液滴进行液滴操作的电极。还提供了输送颗粒的方法，其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。

72. 发明申请

US20070264675A1 Microfluidic device for cell separation and uses thereof 有权
公开(公告)号：US20070264675A1
公开(公告)日：2007-11-15
申请号：US11800940
申请日：2007-05-08
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
IPC分类号： C12Q1/02 , G01N33/53
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).

73. 发明授权

US07294503B2 Microfabricated crossflow devices and methods 有权
标题翻译：微型横流装置和方法
公开(公告)号：US07294503B2
公开(公告)日：2007-11-13
申请号：US09953103
申请日：2001-09-14
申请人： Stephen R. Quake , Todd Thorsen
发明人： Stephen R. Quake , Todd Thorsen
IPC分类号： C12M1/34 , G01N33/50
CPC分类号： C12Q1/68 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2200/12 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/12 , B01L2300/123 , B01L2400/0418 , B01L2400/0421 , B01L2400/0454 , B01L2400/0481 , B01L2400/0484 , B01L2400/0487 , B01L2400/0638 , C12M1/34 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N35/08 , G01N2015/0065 , G01N2015/0088 , G01N2015/1006 , G01N2015/1081 , G01N2015/1411 , G01N2015/1481 , G01N2015/149 , Y10T436/118339 , Y10T436/143333
摘要： A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly.
摘要翻译：提供了用于分析和/或分选生物材料的微流体装置（例如分子，例如多核苷酸和多肽，包括蛋白质和酶;病毒和细胞）及其使用方法。本发明的装置和方法可用于分选颗粒，例如病毒粒子。本发明也可用于高通量筛选，例如组合筛选微流体装置包括在液滴挤出区域处与主通道连通的主通道和入口区域。含有生物材料的溶液的液滴通过液滴挤出区沉积到主通道中。与含有生物材料的溶液不同且不相容的流体流过主通道，使得含有生物材料的液滴不会扩散或混合。可以通过检测每个液滴中的生物样品的预定特征并相应地分选液滴来分析和/或分选液滴内的生物材料。

74. 发明申请

US20070207548A1 Microflow System for Particle Separation and Analysis 审中-公开
标题翻译：用于颗粒分离和分析的微流体系统
公开(公告)号：US20070207548A1
公开(公告)日：2007-09-06
申请号：US11560824
申请日：2006-11-16
申请人： Gert Blankenstein
发明人： Gert Blankenstein
IPC分类号： G01N33/48
CPC分类号： B03C5/028 , B01D57/02 , B01J19/0093 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0647 , B01L2200/0652 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/043 , B01L2400/0487 , B01L2400/084 , B03C1/035 , B03C5/026 , G01N15/1031 , G01N15/1056 , G01N15/1484 , G01N33/56966 , G01N2015/1006 , G01N2015/1081 , G01N2015/149
摘要： A micro flow system is provided for separating particles, comprising a microfabricated member having a flow channel (5) defined therein for guiding a flow of a fluid containing the particles through the flow channel, first inlet means (2) positioned at one end of the flow channel for entering the fluid into the flow channel, first outlet means (7) positioned at the other end of the flow channel for discharging the fluid from the flow channel, the flow of the fluid containing the particles being controlled in such a way that one particle at the time passes a cross section of the flow channel, the member being positioned in a field that is substantially perpendicular to a longitudinal axis of the flow channel so that particles residing in the flow channel and being susceptible to the field across the flow channel are deflected in the direction of the field. Further, a micro flow system is provided for analyzing components of a fluid comprising a microfabricated member having a flow channel defined therein for guiding a flow of a fluid through the flow channel, first inlet means for entering particles into the flow channel, first outlet means for discharging of fluid from the flow channel and a plurality of assay sites located in the flow channel and comprising immobilized reagents whereby the fluid may be analyzed for a plurality of components while residing in the flow channel.
摘要翻译：提供微流量系统用于分离颗粒，包括微型构件，其具有限定在其中的流动通道（5），用于引导包含颗粒的流体通过流动通道，第一入口装置（2）位于用于将流体进入流动通道的流动通道，位于流动通道的另一端的第一出口装置（7），用于从流动通道排出流体，包含颗粒的流体的流动被控制为使得一个颗粒此时通过流动通道的横截面，该构件定位在基本上垂直于流动通道的纵向轴线的场中，使得位于流动通道中的颗粒易于流过流动的场通道沿场的方向偏转。此外，提供了一种微流量系统，用于分析流体的部件，该流体包括具有限定在其中的流动通道的微型构件，用于引导流体流过流动通道，用于将颗粒进入流动通道的第一入口装置，第一出口装置用于从流动通道排放流体和位于流动通道中的多个测定位点，并且包括固定的试剂，由此可以在流体在流动通道中同时分析多种组分。

75. 发明授权

US07258774B2 Microfluidic devices and methods of use 有权
标题翻译：微流体装置及使用方法
公开(公告)号：US07258774B2
公开(公告)日：2007-08-21
申请号：US09970122
申请日：2001-10-02
申请人： Hou-Pu Chou , Anne Y. Fu , Stephen R. Quake
发明人： Hou-Pu Chou , Anne Y. Fu , Stephen R. Quake
IPC分类号： F04B43/00
CPC分类号： F04B43/00 , A61M2206/22 , B01L3/5027 , B01L3/50273 , B01L3/502738 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2300/123 , B01L2400/0418 , B01L2400/0481 , B01L2400/0655 , B01L2400/084 , F04B11/00 , F04B19/006 , F04B43/043 , G01N30/6095 , G01N2015/1081 , G01N2015/149 , G01N2030/205 , G01N2030/322 , Y10T436/2575
摘要： A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality.
摘要翻译：微流体装置包括泵，阀和流体振荡阻尼器。在用于排序的装置中，实体通过泵通过检测区域的流动通道流到结。基于在检测区域中确定的实体的身份，致动位于连接处的流动通道的相对分支上的废物或收集阀，从而将该实体路由到废物池或收集池。阻尼器结构可以位于泵和接头之间。阻尼器由于泵的操作而减小了流动通道中的振荡压力的振幅，从而减少了分离过程中实体的速度振荡。微流体装置可以形成为弹性体材料块，弹性体材料的薄膜可偏转到流动通道中以提供泵或阀的功能。

76. 发明申请

US20070172903A1 Microfluidic device for cell separation and uses thereof 有权
公开(公告)号：US20070172903A1
公开(公告)日：2007-07-26
申请号：US11726230
申请日：2007-03-21
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
IPC分类号： G01N33/574 , C12M3/00
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).

77. 发明申请

US20070125941A1 MICROFLUIDIC DEVICE FOR CELL AND PARTICLE SEPARATION 有权
标题翻译：用于细胞和颗粒分离的微流体装置
公开(公告)号：US20070125941A1
公开(公告)日：2007-06-07
申请号：US11670945
申请日：2007-02-02
申请人： Abraham Lee , Lisen Wang , Lisa Flanagan Monuki , Edwin Monuki , Svyatoslav Marchenko
发明人： Abraham Lee , Lisen Wang , Lisa Flanagan Monuki , Edwin Monuki , Svyatoslav Marchenko
IPC分类号： H05H3/02 , H01S1/00 , H01S3/00
CPC分类号： B01L3/502761 , B01L2200/0647 , B01L2300/0829 , B01L2300/0864 , B01L2400/0424 , B03C5/005 , B03C5/026 , C12M23/16 , C12M33/00 , G01N15/1056 , G01N2015/1081
摘要： A microfluidic separation device includes a microchannel formed in a substrate and being defined at least by a bottom surface, a first side wall, and second side wall. Fluid containing particles or cells is flowed through the microchannel from an upstream end to a downstream end. The downstream end terminates in a plurality of branch channels. A plurality of vertically-oriented electrodes are disposed on the first wall and on the second wall opposite to the first wall. A voltage source is connected to the plurality of opposing electrodes to drive the electrodes. The opposing, vertically-oriented electrodes may be used to focus a heterogeneous population of particles or cells for subsequent downstream separation via additional electrodes placed on one of the side walls. Alternatively, the opposing, vertically-oriented electrodes may be used to spatially separate a heterogeneous population of particles or cells for later collection in one or more of the branch channels.
摘要翻译：微流体分离装置包括形成在基板中并且至少由底面，第一侧壁和第二侧壁限定的微通道。含有流体的颗粒或细胞通过微通道从上游端流到下游端。下游端终止于多个分支信道。多个垂直取向的电极设置在第一壁上和与第一壁相对的第二壁上。电压源连接到多个相对电极以驱动电极。相对的垂直取向的电极可以用于通过放置在其中一个侧壁上的附加电极来聚焦异质粒子或细胞群，用于随后的下游分离。或者，可以使用相对的垂直取向的电极在空间上分离多个粒子或细胞的不同群体，用于以后在一个或多个分支通道中进行收集。

78. 发明申请

US20060172315A1 Methods for staining cells for identification and sorting 审中-公开
标题翻译：染色细胞进行鉴定和分选的方法
公开(公告)号：US20060172315A1
公开(公告)日：2006-08-03
申请号：US11048101
申请日：2005-02-01
申请人： Amy Anderson , Christopher Knutson , Daniel Mueth , Joseph Plewa , Evan Tanner
发明人： Amy Anderson , Christopher Knutson , Daniel Mueth , Joseph Plewa , Evan Tanner
IPC分类号： C12Q1/68 , G01N33/48
CPC分类号： C12N5/0612 , A61D19/04 , B82Y5/00 , B82Y10/00 , C12N5/061 , G01N1/30 , G01N15/14 , G01N15/1425 , G01N21/6428 , G01N33/5005 , G01N33/689 , G01N2015/0065 , G01N2015/1006 , G01N2015/1081 , G01N2015/149 , G01N2021/6439
摘要： The present invention provides novel methods of cell staining, such as bovine sperm, using electroporation or osmolality treatments at viability-enhancing temperatures. Furthermore, methods of highly efficient cell sorting that are especially suitable in sorting bovine sperm using novel cell staining procedures are also provided.
摘要翻译：本发明提供新的细胞染色方法，例如牛精子，在活力增强温度下使用电穿孔或渗透压浓度处理。此外，还提供了使用新型细胞染色方法特别适用于分选牛精子的高效细胞分选方法。

79. 发明申请

US20050079600A1 Mems-bseed integrated magnetic particle identification system 审中-公开
标题翻译： Mems-bseed集成磁粉识别系统
公开(公告)号：US20050079600A1
公开(公告)日：2005-04-14
申请号：US10626221
申请日：2003-07-24
申请人： Aaron Fleischman , Shuvo Roy , Jeff Chalmers , Maciej Zborowski
发明人： Aaron Fleischman , Shuvo Roy , Jeff Chalmers , Maciej Zborowski
IPC分类号： B81B1/00 , G01N15/10 , G01N15/14 , G01N33/543 , C12M1/34 , G01N33/553
CPC分类号： G01N33/54326 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/043 , B01L2400/0487 , C12M47/04 , G01N15/1031 , G01N15/1056 , G01N15/1456 , G01N2015/1006 , G01N2015/1062 , G01N2015/1081
摘要： The present invention provides a MEMS-based integrated particle identification system having a substrate, a magnetic structure, and a bioferrograph. The substrate includes a topside portion, backside portion and a flow system. The flow system includes a flow channel for accepting the flow of a stream of particles to identified. The magnetic structure is in physical communication with the topside and backside portions of the substrate and has at least two pole pieces. A plurality of pole piece embodiments are provided for generating a magnetic field that acts on magnetically susceptible particles in the flow stream. The bioferrograph has at least one sensor for identifying the presence and quantity of magnetically susceptible particles. A plurality of sensor embodiments are also provided.
摘要翻译：本发明提供了一种基于MEMS的集成粒子识别系统，该系统具有基底，磁性结构和生物计量学。衬底包括顶侧部分，后侧部分和流动系统。流动系统包括用于接收所识别的颗粒物流的流动通道。磁性结构与衬底的顶侧和后侧部分物理连通，并具有至少两个极片。提供了多个极片实施例，用于产生作用在流动流中的易磁敏颗粒上的磁场。生物传感器具有至少一个用于识别磁敏感颗粒的存在和数量的传感器。还提供了多个传感器实施例。

80. 发明授权

US06790330B2 Systems and methods for cell subpopulation analysis 有权
标题翻译：细胞亚群分析的系统和方法
公开(公告)号：US06790330B2
公开(公告)日：2004-09-14
申请号：US09883110
申请日：2001-06-14
申请人： Peter Gascoyne , Jody V. Vykoukal , Frederick F. Becker
发明人： Peter Gascoyne , Jody V. Vykoukal , Frederick F. Becker
IPC分类号： G01N27447
CPC分类号： B03C5/026 , B03C5/028 , G01N15/1056 , G01N27/44786 , G01N30/0005 , G01N2015/0288 , G01N2015/1006 , G01N2015/1081
摘要： Systems, apparatuses and methods for cell isolation and analysis. Cells are introduced into a dielectrophoretic prefilter including one or more trapping electrodes configured to trap at least a portion of the cells with a dielectrophoretic force. The cells trapped from the prefilter are directed into a dielectrophoretic field-flow fractionation separator coupled to the prefilter. The cells are discriminated by balancing a dielectrophoretic force (and optionally a magnetophoretic force) with a gravitational force to displace the cells to positions within a velocity profile in the separator. At least a portion of the cells are trapped as a function of the cells' time of emergence from the separator with two or more spiral electrode segments coupled to the separator.
摘要翻译：用于细胞分离和分析的系统，装置和方法。将细胞引入到介电电泳预过滤器中，其包括一个或多个捕获电极，其被配置为用介电电泳力捕获至少一部分细胞。从预过滤器捕获的细胞被引导到耦合到预过滤器的介电泳流场分馏分离器中。通过平衡介电电泳力（和任选的磁导率）与重力来区分细胞，以将细胞置换到分离器中的速度分布内的位置。细胞的至少一部分作为细胞从分离器出现的时间的函数被捕获，其中两个或更多个螺旋电极段连接到分离器。

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