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71. 发明申请

US20090263837A1 METHOD OF MINIMIZING REAGENT CONSUMPTION IN MICROPLATE-BASED REACTIONS 有权
标题翻译：最小化基于微波反应的试剂消耗的方法
公开(公告)号：US20090263837A1
公开(公告)日：2009-10-22
申请号：US11913052
申请日：2006-04-28
申请人： Masato Mitsuhashi , Mieko Ogura
发明人： Masato Mitsuhashi , Mieko Ogura
IPC分类号： G01N33/53 , G01N33/543 , C12P19/34
CPC分类号： B01L3/50853 , B01L2300/046 , B01L2300/0654 , C12Q1/6806 , Y10S436/809 , C12Q2527/137
摘要： A method is provided for performing a reaction, such as the synthesis of concentrated cDNA, in the wells of a microplate while minimizing the volume of the solution of reagents required to perform the reaction. In the method, a pestle is inserted into the well of a microplate to which a substance has been immobilized. A volume of reagent solution is introduced into the well that is insufficient to cover the portion of the well onto which the substance is immobilized. The insertion of the pestle displaces reagent solution and increases the surface area of the solution in contact with the portion of the well to which the substance has been immobilized when the pestle is inserted.
摘要翻译：提供了一种方法，用于在微量培养板的孔中进行诸如合成浓缩cDNA的反应，同时使进行反应所需的试剂溶液的体积最小化。在该方法中，将杵插入已经固定有物质的微孔板的孔中。将一定体积的试剂溶液引入孔中，其不足以覆盖固定有物质的孔的部分。当插入杵时，杵的插入取代试剂溶液并增加溶液的表面积，该表面积与物质固定的孔的部分接触。

72. 发明申请

US20090215064A1 QUANTITATIVE ASSESSMENT OF INDIVIDUAL CANCER SUSCEPTIBILITY BY MEASURING DNA DAMAGE-INDUCED MRNA IN WHOLE BLOOD 审中-公开
标题翻译：通过测量DNA损伤诱导的MRNA在全血中定量评估个体癌的可能性
公开(公告)号：US20090215064A1
公开(公告)日：2009-08-27
申请号：US12395315
申请日：2009-02-27
申请人： Masato Mitsuhashi , Hoda Anton-Culver , Argyrios Ziogas , David Peel
发明人： Masato Mitsuhashi , Hoda Anton-Culver , Argyrios Ziogas , David Peel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/158
摘要： Heparinized human whole blood from patients with invasive breast cancer, with (multiple primary) and without (single primary) a second primary cancer, and from unaffected controls was stimulated with 0.1-10 Gy of radiation and incubated at 37° C. for 2 hours. P21 and PUMA mRNA were then quantified. The results suggest that cancer susceptibility represented by the multiple primary cases was significantly related to over-reaction of p21 mRNA, and not PUMA.
摘要翻译：用具有（多原发）和无（单次原发）第二原发性癌症的患者和来自未受影响的对照的患者进行肝素化的全血用0.1-10Gy的辐射刺激，并在37℃下温育2小时。然后定量P21和PUMA mRNA。结果表明，多发性原发性癌症易感性与p21 mRNA，而不是PUMA的过度反应有显着相关性。

73. 发明申请

US20090011410A1 METHOD FOR TAILORING ADMINISTRATION OF DRUGS BY QUANTITATION OF MRNA 审中-公开
标题翻译：通过MRNA量化来定量给药的方法
公开(公告)号：US20090011410A1
公开(公告)日：2009-01-08
申请号：US11577683
申请日：2005-10-20
申请人： Masato Mitsuhashi
发明人： Masato Mitsuhashi
IPC分类号： C12Q1/68
CPC分类号： G01N33/57426 , C12Q1/6886 , C12Q2600/158 , G01N2333/4724
摘要： The present invention discloses a method for tailoring drug protocols to individual patients based on the levels of marker mRNA measured in leukocytes after stimulation of whole blood of the patient with candidate drugs. A method of measuring a patient's responsiveness to a drug is disclosed that includes exposing whole blood of the patient to the drug for 7 hours or less; after the exposure, measuring the amount of an mRNA associated with an effect of the drug in blood cells; and identifying responsiveness to the drug based on the results of the measurement, wherein a change in the amount of the mRNA indicates the patient's responsiveness to the drug. The amount of mRNA measured in the blood cells may be compared with the level of mRNA present in the cells before exposure or with the level of mRNA present in cells exposed for the same amount of time to a control vehicle. Marker mRNAs useful in the present invention include mRNAs encoding the gene product of the p21, BAX, PUMA, NOXA, and IL-2 genes. The method may be employed for patients with, among other conditions, cancer or diseases or conditions requiring immunosuppression.
摘要翻译：本发明公开了一种基于在患有候选药物的患者的全血刺激后在白细胞中测量的标记物mRNA水平对个体患者定制药物方案的方法。公开了一种测量患者对药物的反应性的方法，其包括将患者的全血暴露于药物7小时以下; 在暴露后，测量与药物在血细胞中的作用相关的mRNA的量; 并且基于测量结果确定对药物的反应性，其中mRNA量的变化表示患者对药物的反应性。可以将在血细胞中测量的mRNA的量与暴露前的细胞中存在的mRNA的水平或者存在于暴露于相同时间量的对照载体的细胞中的mRNA水平进行比较。可用于本发明的标记mRNA包括编码p21，BAX，PUMA，NOXA和IL-2基因的基因产物的mRNA。除了其他病症之外，该方法可用于需要免疫抑制的癌症或疾病或病症的患者。

74. 发明申请

US20080009009A1 Device and method for high-throughput quantification of mRNA from whole blood 有权
标题翻译：从全血中高通量定量mRNA的装置和方法
公开(公告)号：US20080009009A1
公开(公告)日：2008-01-10
申请号：US11803663
申请日：2007-05-15
申请人： Masato Mitsuhashi
发明人： Masato Mitsuhashi
IPC分类号： C12Q1/68 , C07H21/02 , C12P19/34
CPC分类号： C12N15/1006 , C12N1/066 , C12Q1/6806 , C12Q1/6846 , C12Q2565/537 , C12Q2521/107 , C12Q2527/137 , C12Q2527/125
摘要： Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.
摘要翻译：公开了一种用于从全血中简单，可重复和高通量定量mRNA的方法，装置试剂盒和自动化系统。更具体地，方法，装置，试剂盒和自动化系统涉及附着于寡聚（dT）活化的多孔板的白细胞过滤器的组合。

75. 发明授权

US07285199B2 Apparatus and method for electrophoretic microspot concentration 有权
标题翻译：电泳微球浓度的仪器和方法
公开(公告)号：US07285199B2
公开(公告)日：2007-10-23
申请号：US10399882
申请日：2001-10-31
申请人： Masato Mitsuhashi , Taku Murakami , Tsuruki Tamura , Masafumi Yohda , Mina Okochi
发明人： Masato Mitsuhashi , Taku Murakami , Tsuruki Tamura , Masafumi Yohda , Mina Okochi
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44704 , B01L3/5025 , B01L2300/0618 , B01L2300/0829 , B01L2400/0421 , G01N27/44756 , G01N2001/4016 , G01N2001/4038
摘要： A method and apparatus for concentrating dilute biomolecules in samples using electrophoresis are described. The method involves using the charged nature of the biomolecules to localize them to a microspot containing a dialysis membrane. The method and apparatus may also be used for identifying the presence of a biomolecule in a mixture or to quantitate the amount of a specific biomolecule in a mixture. The method and apparatus may also be used to produce a high throughput method for analysis and quantitation of a sample containing biomolecules.
摘要翻译：描述了使用电泳浓缩样品中的稀释生物分子的方法和装置。该方法包括使用生物分子的带电性质将其定位于含有透析膜的微管。该方法和装置也可用于鉴定混合物中生物分子的存在或定量混合物中特定生物分子的量。该方法和装置还可用于产生用于分析和定量含有生物分子的样品的高通量方法。

76. 发明授权

US07214781B2 Gene markers for lung cancer 有权
标题翻译：肺癌基因标记
公开(公告)号：US07214781B2
公开(公告)日：2007-05-08
申请号：US10297277
申请日：2001-06-21
申请人： Masato Mitsuhashi , Hiroko Matsunaga , Hideki Kambara , Masafumi Kawamura
发明人： Masato Mitsuhashi , Hiroko Matsunaga , Hideki Kambara , Masafumi Kawamura
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C07H21/04 , C07K16/30 , C12Q1/6809 , C12Q1/686 , C12Q1/6886 , C12Q2600/158 , G01N33/57423 , G01N33/57484 , C12Q2539/113 , C12Q2537/162 , C12Q2525/191 , C12Q2525/15
摘要： A method for the diagnosis and identification of new or residual lung cancer is disclosed which uses newly identified markers for lung cancer including syndecan 1, collagen 1 alpha 2, and two novel proteins, 7013 and 7018. The method involves identification of the lung cancer markers is blood from a patient. It is envisioned that at least one marker may be used or any mixture of the four. The method may also include the identification of cytokeratin-19.
摘要翻译：公开了用于诊断和鉴定新的或残留的肺癌的方法，其使用新鉴定的肺癌标志物，包括syndecan 1，胶原1α2和两种新型蛋白质，7013和7018.该方法涉及鉴定肺癌标志物是来自病人的血液。可以想象，可以使用至少一个标记物或四种的任何混合物。该方法还可以包括细胞角蛋白-19的鉴定。

77. 发明授权

US5617310A Multiple operation mode microcontroller 失效
标题翻译：多工作模式微控制器
公开(公告)号：US5617310A
公开(公告)日：1997-04-01
申请号：US405016
申请日：1995-03-16
申请人： Masato Mitsuhashi
发明人： Masato Mitsuhashi
IPC分类号： G06F15/78 , G01R31/317 , G06F9/318 , G06F9/38 , G05B11/01
CPC分类号： G06F9/30189 , G01R31/31701 , G06F9/3869
摘要： A microcontroller capable of operating in any one of several operation modes and includes first latch circuits which latch first mode signals from first external nodes at a first timing and second latch circuits latching second mode signals from the same external nodes at a second timing. Operation modes are switched by using the mode signals stored in the first and second latch circuits, so that a single external node can provide two bits for expressing the operation modes. Thus, the number of bits used to represent the mode signal can be increased without increasing the number of external nodes. Also provided is a microcontroller in which the time required for a test can be reduced by eliminating the waiting period for clock generation to be stabilized.
摘要翻译：一种微控制器，其能够以多种操作模式中的任何一种运行，并且包括在第一定时从第一外部节点锁存第一模式信号的第一锁存电路，以及在第二定时将第二模式信号从相同外部节点锁存的第二锁存电路。通过使用存储在第一和第二锁存电路中的模式信号来切换操作模式，使得单个外部节点可以提供用于表示操作模式的两个位。因此，可以增加用于表示模式信号的比特数，而不增加外部节点的数量。还提供了一种微控制器，其中可以通过消除时钟产生的等待时间来稳定，可以减少测试所需的时间。

78. 发明授权

US5554502A Process for determining nuclease activity 失效
标题翻译：确定核酸酶活性的方法
公开(公告)号：US5554502A
公开(公告)日：1996-09-10
申请号：US147936
申请日：1993-11-05
申请人： Masato Mitsuhashi , Mieko Ogura
发明人： Masato Mitsuhashi , Mieko Ogura
IPC分类号： C12M1/34 , C12Q1/44 , C12Q1/00 , C12N15/00 , C12Q1/68 , G01N21/76
CPC分类号： C12Q1/44 , G01N2333/922 , Y10S436/80
摘要： The present invention relates to analyzing a test sample for the presence or absence of nuclease activity, and for quantifying the amount of nuclease activity. In one aspect of the invention, a kit is provided which allows the rapid and efficient quantification of nuclease activity in a sample having an unknown nuclease activity. In another aspect of the invention, a method for determining the presence or absence of nuclease activity, and for quantifying nuclease activity is disclosed.
摘要翻译：本发明涉及分析测试样品中核酸酶活性的存在或不存在以及用于量化核酸酶活性的量。在本发明的一个方面，提供了一种试剂盒，其允许在具有未知核酸酶活性的样品中快速且有效地定量核酸酶活性。在本发明的另一方面，公开了确定核酸酶活性的存在或不存在以及用于定量核酸酶活性的方法。

79. 发明授权

US4853848A Block access system using cache memory 失效
标题翻译：使用高速缓存的块访问系统
公开(公告)号：US4853848A
公开(公告)日：1989-08-01
申请号：US163911
申请日：1988-03-03
申请人： Masato Mitsuhashi , Takeshi Kitahara
发明人： Masato Mitsuhashi , Takeshi Kitahara
IPC分类号： G06F12/08 , G06F13/12
CPC分类号： G06F12/0879
摘要： A block access system using a cache memory comprises a first control circuit for producing a block access request for requesting read-out of all data included in a block of a predetermined size in response to an access request from an operation unit and inputting the data read out from a main memory unit into a cache memory, and a second control circuit for reading out data from the main memory unit and sending back to the first control circuit a response signal which indicates any one of execution and cancellation of the requested block access in response to the block access request from the first control means. Memory addresses necessary for reading out all the data in one block are produced by the first control circuit when the block access request is cancelled. Alternatively, memory addresses are supplied by the second control circuit when the block access request is executed.

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