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61. 发明授权

US6127153A Method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and gene encoding a polyglycosyltransferase 失效
标题翻译：用多糖基转移酶，多糖基转移酶和编码多糖基转移酶的基因转移至少两个糖单元的方法
公开(公告)号：US6127153A
公开(公告)日：2000-10-03
申请号：US478140
申请日：1995-06-07
申请人： Karl F. Johnson , Stephen Roth , Stephanie L. Buczala
发明人： Karl F. Johnson , Stephen Roth , Stephanie L. Buczala
IPC分类号： C12N15/09 , C12N1/21 , C12N9/10 , C12P19/18 , C12P19/26 , C12R1/00 , C12P1/04
CPC分类号： C12P19/18 , C12N9/1048 , Y10S435/871
摘要： The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.
摘要翻译：本发明涉及用多糖基转移酶，多糖基转移酶和编码这种多糖基转移酶的基因转移至少两个糖单元的方法。

62. 发明授权

US5618541A Vaccine against Neisseria meningitidis infections 失效
标题翻译：疫苗针对脑膜炎奈瑟菌感染
公开(公告)号：US5618541A
公开(公告)日：1997-04-08
申请号：US66167
申请日：1993-06-02
申请人： Marie-Jose Quentin-Millet
发明人： Marie-Jose Quentin-Millet
IPC分类号： A61K39/095 , A61K38/00 , A61P31/04 , C07K1/22 , C07K14/195 , C07K14/22 , C07K14/41 , C07K14/705 , C12P21/02 , C12R1/36 , A61K31/075
CPC分类号： C07K14/22 , A61K38/00 , Y10S435/871
摘要： A vaccinal pharmaceutical composition which comprises, as therapeutic agents, at least a first and a second molecule capable of binding to human transferrin; the said first molecule originating from a first strain of N. meningitidis which possesses a human transferrin receptor in which the lower molecular weight subunit (Tbp2) is recognised by an antiserum to the receptor of N. meningitidis strain 2394 (receptor 2394) and is not recognised by an antiserum to the receptor of N. meningitidis strain 2169 (receptor 2169); and at least a second molecule originating from a second strain of N. meningitidis which possesses a human transferrin receptor in which the lower molecular weight subunit (Tbp2) is recognised by an anti-receptor 2169 antiserum and is not recognised by an anti-receptor 2394 antiserum.
摘要翻译： PCT No.PCT / FR92 / 00905 Sec。 371日期：1993年6月2日 102（e）日期1993年6月2日PCT提交1992年9月29日PCT公布。出版物WO93 / 06861 日期：1993年04月15日一种疫苗药物组合物，其包含至少一种能够结合人转铁蛋白的第一和第二分子作为治疗剂; 所述第一分子源自具有人类转铁蛋白受体的脑膜炎奈瑟氏球菌的第一菌株，其中低分子量亚基（Tbp2）被脑膜炎奈瑟氏球菌菌株2394（受体2394）的受体的抗血清识别，并且不是被脑膜炎奈瑟氏球菌菌株2169（受体2169）的受体的抗血清识别; 和至少一种源自第二脑膜炎奈瑟氏球菌菌株的第二分子，其具有人类转铁蛋白受体，其中低分子量亚基（Tbp2）被抗受体2169抗血清识别，并且不被抗受体2394识别抗血清

63. 发明授权

US5556755A Method for detecting Branhamella catarrhalis 失效
标题翻译：用于检测粘膜炎白念珠菌的方法
公开(公告)号：US5556755A
公开(公告)日：1996-09-17
申请号：US129719
申请日：1993-09-29
申请人： Timothy F. Murphy
发明人： Timothy F. Murphy
IPC分类号： A61K38/00 , A61K39/00 , C07K14/21 , C12Q1/68 , C07H21/04 , C12N15/00 , C12P19/34
CPC分类号： C07K14/212 , A61K38/00 , A61K39/00 , Y10S435/871
摘要： Compositions comprising outer membrane protein "CD", and peptides thereof, of Branhamella catarrhalis are described. Additionally, nucleotide sequences encoding the protein or peptide are disclosed, as well as recombinant vectors containing these sequences. Protein or peptide can be produced from host cell systems containing these recombinant vectors. Peptides can also be chemically synthesized. Disclosed are the uses of the protein and peptides as antigens for vaccine formulations, and as antigens in diagnostic immunoassays. The nucleotide sequences are useful for inserting into a viral vector in constructing a recombinant viral vaccine. Also described is the use of nucleotide sequences related to the gene encoding CD as primers and/or probes in molecular diagnostic assays for the detection of B. catarrhalis.
摘要翻译：描述了包含布列塔门氏菌属的外膜蛋白“CD”及其肽的组合物。此外，公开了编码蛋白质或肽的核苷酸序列，以及含有这些序列的重组载体。可以从含有这些重组载体的宿主细胞系统产生蛋白质或肽。肽也可以化学合成。公开了蛋白质和肽作为疫苗制剂的抗原以及作为诊断性免疫测定中的抗原的用途。核苷酸序列可用于在构建重组病毒疫苗中插入病毒载体。还描述了在分子诊断测定中使用与编码CD的基因作为引物和/或探针相关的核苷酸序列用于检测卡他莫拉菌。

64. 发明授权

US5525717A Support-bound nucletide probe for neisseria gonorrhoeae 失效
标题翻译：淋病奈瑟氏球菌支架结合核苷酸探针+ B
公开(公告)号：US5525717A
公开(公告)日：1996-06-11
申请号：US83946
申请日：1993-06-25
申请人： Charles G. Miyada , Teresa L. Born
发明人： Charles G. Miyada , Teresa L. Born
IPC分类号： C07K7/06 , C07K16/12 , C12N15/31 , C12Q1/68 , C07H21/00 , C12P19/34
CPC分类号： C12Q1/689 , Y10S435/822 , Y10S435/871
摘要： A nucleotide sequence characteristic of Neisseria gonorrhoeae is disclosed. The sequence can be the basis for hybridization type, nucleic acid-based, rapid, in vitro diagnostic assays. The unique nature of the sequence makes it possible to clearly discriminate N. gonorrhoeae from other Neisseria species thus eliminating or substantially reducing the number of false positive readings. A 350 base pair N. gonorrhoeae DNA restriction fragment was cloned after subtractive hybridization to Neisseria meningitidis DNA. In further cloning experiments the sequences adjacent to the original 350 base pair fragment were determined. A portion of this sequence was shown to detect 105 of 106 N. gonorrhoeae strains and no other Neisseria species. In addition to use as detection probes, all or portions of the nucleotide sequence can be used as a ligand for the sandwich capture of N. gonorrhoeae sequences and as primers for in vitro amplification of N. gonorrhoeae sequences. The polypeptides encoded by the presently disclosed sequence, including antibodies thereto, are also disclosed as are their uses.
摘要翻译：披露了淋病奈瑟氏球菌特征的核苷酸序列。该序列可以是杂交类型，基于核酸，快速，体外诊断测定的基础。序列的独特性质使得可以清楚地区分淋病奈瑟氏球菌与其他奈瑟氏菌属物种，从而消除或显着减少假阳性读数的数量。在与奈瑟氏球菌脑膜炎奈瑟菌DNA消减杂交后克隆了350碱基对淋病奈瑟氏球菌DNA限制性片段。在进一步克隆实验中，确定与原始350碱基对片段相邻的序列。该序列的一部分显示检测到106个淋病奈瑟氏球菌菌株中的105个，并且没有其他的奈瑟球菌属物种。除了用作检测探针之外，核苷酸序列的全部或部分可以用作奈氏淋病奈瑟氏球菌序列的夹心捕获的配体，并且用作用于体外扩增淋病奈瑟氏球菌序列的引物。本文公开的序列编码的多肽（包括其抗体）也被用于其用途。

65. 发明授权

US5264346A Colorimetric method for beta-lactamase assay and its applications 失效
标题翻译： β-内酰胺酶测定法比色法及其应用
公开(公告)号：US5264346A
公开(公告)日：1993-11-23
申请号：US710510
申请日：1991-06-03
申请人： Kirk C. S. Chen
发明人： Kirk C. S. Chen
IPC分类号： G01N31/22 , C12Q1/26 , C12Q1/28
CPC分类号： G01N31/22 , Y10S435/871 , Y10S435/933 , Y10S435/973 , Y10S436/904
摘要： A novel colorimetric method for beta-lactamase assay and its applications are disclosed. The novel assay method for beta-lactamase is based on the discovery described in this invention. The chromophore formed by oxidation of either the N-alkyl derivative of p-phenylenediamine or the 3,3',5,5'-tetraalkyl derivative of benzidine can be decolorized by the open beta-lactam ring end product resulting from the hydrolysis of a penicillin in the presence of a mercury-containing compound. The same chromophore can be decolorized by the open beta-lactam ring end product resulting from the hydrolysis of a cephalosporin in either the presence or the absence of the mercury-containing compound. None of the intact beta-lactam antibiotics can decolorize the chromophore in either the presence or the absence of the mercury-containing compound. The final concentration of the mercury-containing compound in the decolorization mixture ranges from approximately 0.005 mM to approximately 3 mM, and the pH of the decolorization mixture ranges from 3.5 to 8.0. Additional applications derived from coupling this novel assay method to methods in the art include: a method for specific detection of microbial beta-lactamases; a method for simultaneous detection of microbial cytochrome c oxidase systems and penicillinases, and its application to simultaneous presumptive identification of Neisseria gonorrhoeae in urethral exudates, and differentiation of the infecting gonococci into penicillinase-producing N. gonorrhoeae and non-penicillinase-producing N. gonorrhoeae; and a simultaneous assay method for peroxidase and penicillinase.
摘要翻译：公开了一种β-内酰胺酶测定法的新型比色法及其应用。 β-内酰胺酶的新型测定方法是基于本发明所述的发现。通过对苯二胺的N-烷基衍生物或联苯胺的3,3'，5,5'-四烷基衍生物的氧化形成的发色团可以由开水的β-内酰胺环末端产物脱色青霉素在含汞化合物存在下。相同的发色团可以通过在含汞化合物的存在或不存在下由头孢菌素水解产生的开放的β-内酰胺环末端产物脱色。没有一种完整的β-内酰胺抗生素可以在存在或不存在含汞化合物的情况下使发色团脱色。脱色混合物中含汞化合物的最终浓度为约0.005mM至约3mM，脱色混合物的pH为3.5至8.0。将该新型测定方法与本领域的方法联用得到的其它应用包括：用于特异性检测微生物β-内酰胺酶的方法; 同时检测微生物细胞色素c氧化酶系统和青霉素酶的方法，以及其在尿道渗出液中同时推测淋病奈瑟氏球菌的鉴定，以及感染性淋球菌分泌为产青青霉素酶的淋病奈瑟氏球菌和产生非青霉素酶的淋病奈瑟氏球菌 ; 以及过氧化物酶和青霉素酶的同时测定方法。

66. 发明授权

US5256536A Nucleotide probe for Neisseria gonrrhoeae 失效
标题翻译：奈瑟氏球菌的核苷酸探针
公开(公告)号：US5256536A
公开(公告)日：1993-10-26
申请号：US611528
申请日：1990-11-09
申请人： Charles G. Miyada , Teresa L. Born
发明人： Charles G. Miyada , Teresa L. Born
IPC分类号： C07K7/06 , C07K16/12 , C12N15/31 , C12Q1/68 , C12G1/68 , C07M15/12 , C12D12/34 , C12Q1/10
CPC分类号： C12Q1/689 , Y10S435/822 , Y10S435/871
摘要： A nucleotide sequence characteristic of Neisseria gonorrhoeae is disclosed. The sequence can be the basis for hybridization type, nucleic acid-based, rapid, in vitro diagnostic assays. The unique nature of the sequence makes it possible to clearly discriminate N. gonorrhoeae from other Neisseria species thus eliminating or substantially reducing the number of false positive readings. A 350 base pair N. gonorrhoeae DNA restriction fragment was cloned after subtractive hybridization to Neisseria meningitidis DNA. In further cloning experiments the sequences adjacent to the original 350 base pair fragment were determined. A portion of this sequence was shown to detect 105 of 106 N. gonorrhoeae strains and no other Neisseria species. In addition to use as detection probes, all or portions of the nucleotide sequence can be used as a ligand for the sandwich capture of N. gonorrhoeae sequences and as primers for in vitro amplification of N. gonorrhoeae sequences. The polypeptides encoded by the presently disclosed sequence, including antibodies thereto, are also disclosed as are their uses.
摘要翻译：披露了淋病奈瑟氏球菌特征的核苷酸序列。该序列可以是杂交类型，基于核酸，快速，体外诊断测定的基础。序列的独特性质使得可以清楚地区分淋病奈瑟氏球菌与其他奈瑟氏菌属物种，从而消除或显着减少假阳性读数的数量。在与奈瑟氏球菌脑膜炎奈瑟菌DNA消减杂交后克隆了350碱基对淋病奈瑟氏球菌DNA限制性片段。在进一步克隆实验中，确定与原始350碱基对片段相邻的序列。该序列的一部分显示检测到106个淋病奈瑟氏球菌菌株中的105个，并且没有其他的奈瑟球菌属物种。除了用作检测探针之外，核苷酸序列的全部或部分可以用作奈氏淋病奈瑟氏球菌序列的夹心捕获的配体，并且用作用于体外扩增淋病奈瑟氏球菌序列的引物。本文公开的序列编码的多肽（包括其抗体）也被用于其用途。

67. 发明授权

US4959303A Assay for antigens by binding immune complexes to solid supports free of protein and non-ionic binders 失效
标题翻译：通过将免疫复合物与不含蛋白质和非离子粘合剂的固体支持物结合来测定抗原
公开(公告)号：US4959303A
公开(公告)日：1990-09-25
申请号：US135869
申请日：1987-12-21
申请人： Gary L. Milburn , Judith Rabbie , Thomas M. Houts
发明人： Gary L. Milburn , Judith Rabbie , Thomas M. Houts
IPC分类号： G01N33/543 , G01N33/569 , G01N33/571
CPC分类号： G01N33/54306 , G01N33/56911 , G01N33/571 , Y10S435/871 , Y10S436/824 , Y10S436/828
摘要： Methods are disclosed for detecting an antigen in a biological sample. The methods involve providing in combination a solid support, which is substantially free of specific binding proteins, and a medium comprising an antigen from the sample and an antibody for the antigen. The combination is incubated under conditions sufficient for the antibody when bound to the antigen to bind to the support. The presence or amount of antibody on the support or in the medium is determined and is related to the presence of antigen in the sample. The methods have particular application to the detection of gram-negative bacteria.

68. 发明授权

US4659658A Lectin-coated latex agglutination assay for Neisseria gonorrhoeae 失效
标题翻译：淋球菌淋巴细胞凝集素胶乳凝集试验
公开(公告)号：US4659658A
公开(公告)日：1987-04-21
申请号：US686190
申请日：1984-12-26
申请人： Laurence R. McCarthy , H. Mark Perks
发明人： Laurence R. McCarthy , H. Mark Perks
IPC分类号： G01N33/571 , G01N33/546 , G01N33/569
CPC分类号： G01N33/571 , Y10S435/81 , Y10S435/871 , Y10S435/975 , Y10S436/808 , Y10S436/827
摘要： A method for identification of a microorganism includes combining in a suitable fluid the microorganism and a latex containing a particle coated with about 1 to 150 .mu.g per ml of latex of a lectin specific for the microorganism. Binding of the microorganism and lectin causes agglutination which is detected. The microorganism is identified by the agglutination as that organism which is specific for the lectin. The invention includes a reagent which may be included with other materials in a kit of materials useful for performing the method of the invention.
摘要翻译：用于鉴定微生物的方法包括在合适的流体中混合微生物和含有涂覆有约1至150μg/ ml的微生物特异性凝集素胶乳的颗粒的胶乳。微生物和凝集素的结合引起检测到的凝集。微生物被凝集鉴定为特异于凝集素的生物体。本发明包括可以在用于执行本发明的方法的一组材料中的其它材料中包含的试剂。

69. 发明授权

US4525452A Enzyme immunoassay with step of immersing sample in deionized water 失效
标题翻译：酶免疫测定，将样品浸入去离子水中
公开(公告)号：US4525452A
公开(公告)日：1985-06-25
申请号：US460330
申请日：1983-01-24
申请人： Wendy Jones , Bego Gerber
发明人： Wendy Jones , Bego Gerber
IPC分类号： C12Q1/10 , G01N33/569 , G01N33/571 , G01N33/54 , C12Q1/04 , C12R1/36
CPC分类号： C12Q1/10 , G01N33/56911 , G01N33/571 , Y10S435/81 , Y10S435/871 , Y10S436/808
摘要： Immunoassay detection of bacterial diseases, bacteria, and microorganisms using a deionized water collection medium. The preferred enzyme immunoassay is of particular use in clinical or home testing application for detection of bacteria such as gonococcus, antigens derived from such bacteria, and antibodies against the bacteria. A colorimetric detection technique may be employed using chromogenic solutions containing tetramethylbenzidine or water soluble derivatives of tetramethylbenzidine.
摘要翻译：使用去离子水收集培养基免疫测定细菌疾病，细菌和微生物。优选的酶免疫测定在临床或家庭测试应用中特别用于检测细菌如淋球菌，源自这种细菌的抗原和针对细菌的抗体。可以使用含有四甲基联苯胺或四甲基联苯胺的水溶性衍生物的显色溶液进行比色检测技术。

70. 发明授权

US4461838A Gonococcal Pili processes for the preparation thereof and the use thereof for the detection of and prevention of infections caused by Neisseria gonorrhoeae 失效
标题翻译：用于其制备的淋球菌Pili方法及其用于检测和预防由淋病奈瑟氏球菌引起的感染的用途
公开(公告)号：US4461838A
公开(公告)日：1984-07-24
申请号：US188463
申请日：1980-09-18
申请人： Charles C. Brinton , John C. McMichael
发明人： Charles C. Brinton , John C. McMichael
IPC分类号： A61K39/00 , C07K14/22 , C07K16/12 , C12P1/04 , C12Q1/12 , G01N33/571 , G01N31/00 , G01N33/48
CPC分类号： G01N33/571 , C07K14/22 , C07K16/1217 , C12P1/04 , C12Q1/12 , A61K39/00 , Y10S435/871 , Y10S435/961
摘要： There are provided a crystalline and single rod products derivable from the Pili of Type 1 and Type 2 Neisseria gonorrhoeae organisms. There are provided methods of growing said organisms to produce the maximum yield of Pili and procedures for purifying said Pili to produce said crystalline material. There are further provided methods of utilizing said Pili to determine the presence, in a system infectable by N. gonorrhoeae organisms, of antibodies to the Pili of said organisms, and methods of serotyping said Pili. There is also provided a mode of utilizing said crystalline material to provide a substantial degree of immunization to infection by N. gonorrhoeae in mammalian systems susceptible to such infection.
摘要翻译：提供了可从1型和2型淋病奈瑟氏球菌生物体的Pili衍生的结晶和单杆产品。提供了使所述生物体生长以产生Pili的最大产量的方法和用于纯化所述Pili以产生所述结晶材料的方法。进一步提供了利用所述Pili来确定由淋病奈瑟氏球菌生物体感染的系统中存在针对所述生物体的皮肤的抗体的方法以及血清分型所述Pili的方法。还提供了利用所述结晶材料以在对这种感染敏感的哺乳动物系统中对淋病奈瑟氏球菌的感染提供显着程度的免疫的模式。

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