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    • 69. 发明授权
    • Material inhibiting lipid peroxide-increase
    • 材料抑制脂质过氧化物增加
    • US6087345A
    • 2000-07-11
    • US234122
    • 1999-01-19
    • Atsutane OhtaMasako OtsukiToshio TakizawaTakashi Adachi
    • Atsutane OhtaMasako OtsukiToshio TakizawaTakashi Adachi
    • A23L29/244A01N43/78
    • A23L29/244
    • A material inhibiting lipid peroxide-increase containing fructooligosaccharides is described which can conveniently provide at a low cost a food or drink product which exerts an excellent effect of inhibiting an increase in lipid peroxides without largely changing the daily dietary contents. The intake of the material inhibiting lipid peroxide-increase of the present invention can provide an excellent means for preventing various diseases (cancer, arterial sclerosis, etc.) caused by the formation of lipid peroxides, retarding aging, etc. Moreover, use of the material inhibiting lipid peroxide-increase of the present invention makes it possible to conveniently provide foods and drinks having excellent effects of inhibiting an increase in lipid peroxides at a low cost.
    • 描述了抑制含有低聚果糖的脂质过氧化物增加的材料,其可以以低成本方便地提供在不大幅度地改变日常饮食含量的情况下发挥抑制脂质过氧化物增加的优异效果的食品或饮料产品。 抑制本发明的脂质过氧化物增加的材料的摄入可以提供用于预防由形成脂质过氧化物引起的各种疾病(癌症,动脉硬化症等),延缓衰老等的优异手段。此外,使用 本发明的抑制脂质过氧化物增加的材料能够以低成本方便地提供具有抑制脂质过氧化物增加的优异效果的食品和饮料。
    • 70. 发明授权
    • Method for obtaining transgenic plants showing a modified fructan pattern
    • 获得显示改性果聚糖图案的转基因植物的方法
    • US6025542A
    • 2000-02-15
    • US446834
    • 1995-08-03
    • Josephus Christianus Maria SmeekensMichael Johannes Marcus EbskampPetrus Jacobus Weisbeek
    • Josephus Christianus Maria SmeekensMichael Johannes Marcus EbskampPetrus Jacobus Weisbeek
    • A01H5/00A23K1/14A23L1/0528C12N5/10C12N9/10C12N15/09C12N15/29C12N15/82C12N15/31C12P19/00
    • A23K10/30A23L29/244C12N15/8246C12N15/8271C12N15/8273C12N9/10
    • The present invention relates to a method for obtaining transgenic plants showing a modified fructan pattern as compared to non-transformed plants, comprising the steps of: a) preparing a DNA construct comprising one or more fructosyltransferase genes, or modified versions thereof, being operably linked to a promoter sequence active in plants and a terminator sequence active in plants; b) transforming a plant cell with the construct; and c) regenerating a transgenic plant from the transformed plant cell. Preferably, the 5' untranslated region of the fructosyltransferase gene is modified such that the expression of the fructosyltransferase is not negatively affected. The DNA construct may also comprise a targeting sequence upstream from the fructosyltransferase gene for directing the fructosyltransferase to a specific plant tissue or plant cell compartment. The fructosyltransferases produced in the transgenic plants change the fructan pattern of the plants, thus leading to different plant performance due to altered sink-source relations and yield, increased tolerance for drought, cold or other stresses, higher dry matter content, better taste and storability, and improved nutritional value. The plants are also suitable for use as raw material for fructan production.
    • PCT No.PCT / NL93 / 00279 Sec。 371日期:1995年8月3日 102(e)日期1995年8月3日PCT 1993年12月28日PCT公布。 公开号WO94 / 14970 日期1994年7月7日本发明涉及一种获得与非转化植物相比显示出改性果聚糖模式的转基因植物的方法,包括以下步骤:a)制备包含一个或多个果糖基转移酶基因或修饰版本的DNA构建体 可操作地连接到在植物中有活性的启动子序列和在植物中活性的终止子序列; b)用植物细胞转化植物细胞; 和c)从转化的植物细胞再生转基因植物。 优选地,对果糖基转移酶基因的5'非翻译区进行修饰,使得果糖基转移酶的表达不受负面影响。 DNA构建体还可以包含来自果糖基转移酶基因上游的靶向序列,用于将果糖基转移酶引导到特定植物组织或植物细胞室。 在转基因植物中产生的果糖基转移酶改变植物的果聚糖模式,从而导致不同的植物性能,这是由于改变的水槽 - 源关系和产量,增加的耐旱性,冷胁迫或其他胁迫,较高的干物质含量,更好的味道和保存性 ,并提高营养价值。 该植物也适用于果聚糖生产的原料。