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61. 发明申请

US20090098627A1 Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule 有权
标题翻译：通过与固定化的脱卤酶底物结合并直接或间接连接到蛋白质或分子的突变体脱卤酶固定蛋白质或分子的方法
公开(公告)号：US20090098627A1
公开(公告)日：2009-04-16
申请号：US12220478
申请日：2008-07-24
申请人： Aldis Darzins , Lance Encell , Tonny Johnson , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
发明人： Aldis Darzins , Lance Encell , Tonny Johnson , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
IPC分类号： C12N11/12 , C12N11/00 , C12N11/14
CPC分类号： C12N9/14 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07K2299/00 , C12N9/0069 , C12Q1/26 , C12Q1/34 , C12Q1/66 , C12Y113/12005 , C12Y308/01005
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译：提供任选融合到感兴趣的蛋白质的突变体水解酶。突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代野生型水解酶。还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

62. 发明申请

US20090068646A1 METHODS AND KITS FOR DETECTING MUTATIONS 审中-公开
标题翻译：检测突变的方法和工具
公开(公告)号：US20090068646A1
公开(公告)日：2009-03-12
申请号：US11577646
申请日：2005-10-24
申请人： Jeffery Bacher , Richard Halberg , Marijo Kent-First , Keith V. Wood
发明人： Jeffery Bacher , Richard Halberg , Marijo Kent-First , Keith V. Wood
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6879 , C12Q1/6858 , C12Q1/6886 , C12Q1/6888 , C12Q1/6897 , C12Q2600/136 , C12Q2600/16 , C12Q2525/151
摘要： Disclosed are methods and kits for detecting mutations in DNA by comparing the size of an amplified microsatellite locus to the expected size. The methods and kits may used in various applications, including monitoring exposure of a cell or organism to a mutagen, evaluating the mutagenicity of an agent, and evaluating a putative precancerous or cancerous cell or tumor cell for microsatellite instability.
摘要翻译：公开了通过将扩增的微卫星位点的大小与预期大小进行比较来检测DNA中的突变的方法和试剂盒。方法和试剂盒可以用于各种应用中，包括监测细胞或生物体对诱变剂的暴露，评估试剂的致突变性，以及评估推定的癌前或癌细胞或肿瘤细胞用于微卫星不稳定性。

63. 发明申请

US20090029398A1 Microbial ATP extraction and detection system 审中-公开
标题翻译：微生物ATP提取和检测系统
公开(公告)号：US20090029398A1
公开(公告)日：2009-01-29
申请号：US12283102
申请日：2008-09-08
申请人： Frank Fan , Braeden Butler , Keith V. Wood
发明人： Frank Fan , Braeden Butler , Keith V. Wood
IPC分类号： C12Q1/66
CPC分类号： C12Q1/66 , C12Q1/008 , C12Q1/02 , C12Q1/04 , G01N33/56916
摘要： The present invention is directed to compositions and methods for single-step extraction and detection of ATP levels from microbial cells. The disclosed compositions are formulated to efficiently elicit bioluminescent detection of ATP among a broad variety of different microorganisms using a common single-step reagent composition. Additional luminescence-based methods are provided for identifying other useful extracting agents or for screening compounds for their pharmaceutical or biological effects on microbial cells.
摘要翻译：本发明涉及用于单步提取和从微生物细胞中检测ATP水平的组合物和方法。所公开的组合物被配制成使用常用的单步试剂组合物在多种不同的微生物中有效地引发ATP的生物发光检测。提供了其他基于发光的方法，用于鉴定其他有用的提取剂或筛选化合物对其对微生物细胞的药物或生物学作用。

64. 发明申请

US20080248511A1 METHODS TO QUENCH LIGHT FROM OPTICAL REACTIONS 审中-公开
标题翻译：从光学反应中排除光的方法
公开(公告)号：US20080248511A1
公开(公告)日：2008-10-09
申请号：US12056073
申请日：2008-03-26
申请人： William Daily , Erika Hawkins , Dieter Klaubert , Mark McDougall , James Unch , Keith V. Wood , Wenhui Zhou , Ji Zhu
发明人： William Daily , Erika Hawkins , Dieter Klaubert , Mark McDougall , James Unch , Keith V. Wood , Wenhui Zhou , Ji Zhu
IPC分类号： C07D209/16 , C12Q1/66
CPC分类号： C12Q1/66
摘要： The present invention relates to single and dual reporter luminescence assays utilizing reagents to quench an optical, e.g., an enzyme-mediated luminescence, reaction. In one embodiment of the invention, a reagent is added to an assay which selectively quenches a first enzyme-mediated luminescence reaction without affecting a subsequent distinct enzyme-mediated luminescent reaction(s). An assay kit containing one or more selective quench reagents, and compositions comprising the quench reagent(s), are also provided.
摘要翻译：本发明涉及使用试剂淬灭光学例如酶介导的发光反应的单和双重报道发光测定。在本发明的一个实施方案中，将试剂加入到选择性淬灭第一酶介导的发光反应而不影响随后不同的酶介导的发光反应的测定中。还提供了含有一种或多种选择性骤冷试剂和包含骤冷试剂的组合物的测定试剂盒。

65. 发明授权

US07429472B2 Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule 有权
标题翻译：通过与固定化的脱卤酶底物结合并直接或间接连接到蛋白质或分子的突变体脱卤酶固定蛋白质或分子的方法
公开(公告)号：US07429472B2
公开(公告)日：2008-09-30
申请号：US11006031
申请日：2004-12-06
申请人： Aldis Darzins , Lance Encell , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
发明人： Aldis Darzins , Lance Encell , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
IPC分类号： C12N9/14 , C12N11/02 , C12N11/14 , C07K14/195
CPC分类号： C12N9/14 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07K2299/00 , C12N9/0069 , C12Q1/26 , C12Q1/34 , C12Q1/66 , C12Y113/12005 , C12Y308/01005
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译：提供任选融合到感兴趣的蛋白质的突变体水解酶。突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代野生型水解酶。还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

66. 发明授权

US07118878B1 Method for increasing luminescence assay sensitivity 有权
标题翻译：增加发光测定灵敏度的方法
公开(公告)号：US07118878B1
公开(公告)日：2006-10-10
申请号：US09590884
申请日：2000-06-09
申请人： Erika Hawkins , John M. Centanni , Jacqueline Sankbeil , Keith V. Wood
发明人： Erika Hawkins , John M. Centanni , Jacqueline Sankbeil , Keith V. Wood
IPC分类号： C12Q1/66
CPC分类号： C12Q1/66 , G01N33/582 , G01N2333/90241 , Y10S435/968 , Y10S435/975
摘要： The invention provides kits and methods for increasing the sensitivity of a bio-luminescent assay, which employ an organic compound that, for instance, reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold and reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold, reduces luminescence generated by luminogenic molecules not bound to an enzyme by at least about 10 fold and reduces the luminescence generated by luminogenic molecules bound to an enzyme by less than about 7 fold, or reduces autoluminescence by at least about 10 fold and reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold.
摘要翻译：本发明提供用于提高生物发光测定的灵敏度的试剂盒和方法，其使用有机化合物，例如将不依赖于分析物的发光的发光减少至少约10倍，并且减少发光，取决于分析物的存在小于约7倍，减少不与酶结合至少约10倍的发光分子产生的发光，并将由酶结合的发光分子产生的发光降低小于约7倍，或将自发光发光降低至少约10倍，并且减少依赖于分析物的存在小于约7倍的发光。

67. 发明授权

US06387675B1 Mutant luciferases 失效
标题翻译：突变荧光素酶
公开(公告)号：US06387675B1
公开(公告)日：2002-05-14
申请号：US08487183
申请日：1995-06-07
申请人： Keith V. Wood , Monika G. Gruber
发明人： Keith V. Wood , Monika G. Gruber
IPC分类号： C12N902
CPC分类号： C12N9/0069 , C12Y113/12007
摘要： The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.
摘要翻译：本发明提供活性的非天然存在的甲虫荧光素酶突变体和编码这种突变体的DNA。本发明的突变荧光素酶与相应的野生型荧光素酶不同，是通过产生与由野生型酶产生的生物发光的峰值强度相差至少1nm的峰强度波长的生物发光。本发明的突变荧光素酶和DNA用于各种生物感测应用中。

68. 发明授权

US5650289A Luciferase assay compositions 失效
标题翻译：萤光素酶测定组合物
公开(公告)号：US5650289A
公开(公告)日：1997-07-22
申请号：US189558
申请日：1994-01-31
申请人： Keith V. Wood
发明人： Keith V. Wood
IPC分类号： G01N21/76 , C12N9/02 , C12Q1/66 , H01L21/306
CPC分类号： C12N9/0069 , C12Q1/66 , C12Y113/12007 , Y10S435/81 , Y10S435/968 , Y10S435/975 , Y10S436/808
摘要： Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions. Thus, the invention provides methods, compositions and test kits for assaying samples for the presence of a beetle luciferase or, using a beetle luciferase-luciferin reaction, the presence of ATP. The invention also provides the complex of the coenzyme, Coenzyme A, a beetle luciferase and oxyluciferin in its exited state in the luciferase-luciferin reaction and luciferyl-CoA, the thioester of Coenzyme A and luciferin (D-(-)-2-(6'-hydroxy-2'-benzothiazolyl)-.DELTA..sup.2 -thiazoline-4-carboxylic acid).
摘要翻译：提供了方法和组合物，用于改善甲虫荧光素酶 - 荧光素反应中荧光素酶活性的光产生动力学。因此，本发明提供了用于测定样品中存在甲虫荧光素酶的方法，组合物和测试试剂盒，或者使用甲虫荧光素酶 - 荧光素反应存在ATP。本发明还提供辅酶A，甲壳素萤光素酶和荧光素酶荧光素酶在荧光素酶荧光素反应中的氧化荧光素与荧光素-CoA的络合物，辅酶A的硫酯和荧光素（D - （ - ） - 2- 6'-羟基-2'-苯并噻唑基） - DELTA 2-噻唑啉-4-羧酸）。

69. 发明授权

US5283179A Luciferase assay method 失效
标题翻译：萤光素酶测定法
公开(公告)号：US5283179A
公开(公告)日：1994-02-01
申请号：US580371
申请日：1990-09-10
申请人： Keith V. Wood
发明人： Keith V. Wood
IPC分类号： G01N21/76 , C12N9/02 , C12Q1/66
CPC分类号： C12N9/0069 , C12Q1/66 , C12Y113/12007 , Y10S435/81 , Y10S435/968 , Y10S435/975 , Y10S436/808
摘要： The invention provides improved methods for assaying samples for the presence of a beetle luciferase. The methods of the invention entail improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions.
摘要翻译：本发明提供了用于测定样品中存在甲壳素萤光素酶的改进方法。本发明的方法需要改进甲虫荧光素酶 - 荧光素反应中荧光素酶活性的光产生动力学。

70. 发明授权

US09816054B2 Cleavable surfactants 有权
公开(公告)号：US09816054B2
公开(公告)日：2017-11-14
申请号：US12249584
申请日：2008-10-10
申请人： Sergei Saveliev , Daniel Simpson , Keith V. Wood , Harry Tetsuo Uyeda , Carolyn Woodroofe Hitko , Dieter Klaubert
发明人： Sergei Saveliev , Daniel Simpson , Keith V. Wood , Harry Tetsuo Uyeda , Carolyn Woodroofe Hitko , Dieter Klaubert
IPC分类号： C07C309/64 , C11D1/20 , C07C309/10 , C07C309/14 , C07C309/15 , C07C309/16 , C07C323/12 , C07C329/06 , C07D307/40 , C07D307/56 , C07D317/22 , C07D333/16 , C11D1/14
CPC分类号： C11D1/20 , C07C309/10 , C07C309/14 , C07C309/15 , C07C309/16 , C07C323/12 , C07C329/06 , C07D307/40 , C07D307/56 , C07D317/22 , C07D333/16 , C11D1/143
摘要： The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.

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