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51. 发明申请

US20070092903A1 One-color microarray analysis methods, reagents and kits 审中-公开
标题翻译：单色微阵列分析方法，试剂和试剂盒
公开(公告)号：US20070092903A1
公开(公告)日：2007-04-26
申请号：US11580581
申请日：2006-10-13
申请人： Stephanie Fulmer-Smentek , Anne Lucas , Erik Bjeldanes , Petula D'Andrade
发明人： Stephanie Fulmer-Smentek , Anne Lucas , Erik Bjeldanes , Petula D'Andrade
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： C12Q1/6837 , C12Q2565/102
摘要： Microarray platforms for performing one-color and two color analyses using a single platform are provided. Methods of using the microarray platforms and analyzing data obtained from such microarrays are also provided.
摘要翻译：提供了使用单个平台执行单色和双色分析的微阵列平台。还提供了使用微阵列平台和分析从这样的微阵列获得的数据的方法。

52. 发明授权

US07157228B2 Genetic analysis and authentication 有权
标题翻译：遗传分析和认证
公开(公告)号：US07157228B2
公开(公告)日：2007-01-02
申请号：US10238439
申请日：2002-09-09
申请人： Ghazala Hashmi , Michael Seul , Joachim Messing
发明人： Ghazala Hashmi , Michael Seul , Joachim Messing
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6827 , B82Y5/00 , B82Y10/00 , B82Y20/00 , C12Q1/6837 , C12Q2565/102 , C12Q2563/149 , C12Q2537/143
摘要： This invention provides compositions and methods for genetic testing of an organism and for correlating the results of the genetic testing with a unique marker that unambiguously identifies the organism. The markers may be internal markers, such as for example single nucleotide polymorphisms (SNPs), short tandem repeats (STRs), or other sites within a genomic locus. Alternatively, the markers may be external, such that they are separately added to the genetic sample before testing.
摘要翻译：本发明提供了用于生物体的遗传测试的组合物和方法，并且用于将遗传测试的结果与明确识别生物体的唯一标记相关联。标记可以是内部标记，例如单核苷酸多态性（SNP），短串联重复序列（STR）或基因组基因座内的其他位点。或者，标记可以是外部的，使得它们在测试之前分开地添加到遗传样品中。

53. 发明申请

US20060177850A1 Particle-based multiplex assay system with three or more assay reporters 审中-公开
标题翻译：具有三个或更多测定记录的基于粒子的多重测定系统
公开(公告)号：US20060177850A1
公开(公告)日：2006-08-10
申请号：US11321374
申请日：2005-12-29
申请人： Mack Schermer , Mark Bobrow , Wayne Patton
发明人： Mack Schermer , Mark Bobrow , Wayne Patton
IPC分类号： C12Q1/68 , C12M1/34 , G01N33/551
CPC分类号： G01N33/54313 , B01J19/0046 , B01J2219/005 , B01J2219/00502 , B01J2219/00504 , B01J2219/00545 , B01J2219/00547 , B01J2219/00576 , B01J2219/00592 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00702 , B01J2219/00722 , C12Q1/6827 , G01N33/54333 , G01N33/54366 , G01N2035/00772 , C12Q2565/102 , C12Q2563/107 , C12Q2537/143 , C12Q2563/149
摘要： A system and method for developing and utilizing particle-based n-multiplexed assays that include three or more reporters utilizes n particle sets that are associated with particle identification images or labels (IDs) that differ between sets. The encoded particles for a given set are coated with a specific binding member, or in the case of the sandwich assay with coupled capture and detector binding pair members, to form particle types. The sets of particle types are then pooled, and aliquots of the particle types are removed to assay vessels. Next, samples with three or four reporter molecules are supplied to the respective vessels. After one or more incubation periods, the particles are supplied to a reader system, which determines the particle IDs to identify the particle types and also detects the reporter signals. The reader system includes multiple excitation lasers that excite the various reporters in sequence or in parallel, to supply associated signals to one or more detectors. Emission filters and wavelength discriminators are included such that a given detector receives at a given time the signals associated with a single assay binding label. The system further develops greater capacity sandwich assays by assigning subsets of capture and detector antibody pairings to the three or four reporters, respectively. The system performs greater numbers of differential RNA expressions based on the use of the three or more reporters, with one or more reporters assigned to the reference sample and the other reporters assigned to respective test samples. The system and method are also capable of performing greater numbers of SNPs utilizing primer extension reactions, by assigning different color reporters to the respective nucleotides or terminators.
摘要翻译：用于开发和利用包括三个或更多个记录器的基于粒子的n多元化测定的系统和方法利用与在组之间不同的粒子识别图像或标签（ID）相关联的n个粒子集。给定组的编码颗粒用特异性结合成员涂覆，或者在使用偶联捕获和检测器结合对成员的夹心测定的情况下，形成颗粒类型。然后将这些颗粒类型集合，并将等分的颗粒类型移除到测定容器中。接下来，将具有三个或四个报告分子的样品供应到各个容器。在一个或多个孵育期后，颗粒被提供给读取器系统，其确定颗粒ID以识别颗粒类型并且还检测报告信号。读取器系统包括多个激发激光器，其按顺序或并联激励各种报告器，以将相关信号提供给一个或多个检测器。包括发射滤波器和波长鉴别器，使得给定检测器在给定时间接收与单个测定结合标签相关联的信号。该系统通过分别将三个或四个记录者的捕获和检测抗体配对分组进一步开发更大容量的夹心测定。该系统基于使用三个或更多个记者，分配给参考样本的一个或多个记者和分配给各个测试样品的其他记者，来执行更多数量的差异RNA表达。通过将不同的颜色记录分配给各个核苷酸或终止子，该系统和方法还能够利用引物延伸反应来进行更多数量的SNP。

54. 发明申请

US20060078898A1 Methods and compositions for reducing label variation in array-based comparative genome hybridization assays II 审中-公开
标题翻译：用于减少基于阵列的比较基因组杂交测定中的标记变异的方法和组合物II
公开(公告)号：US20060078898A1
公开(公告)日：2006-04-13
申请号：US10964504
申请日：2004-10-12
申请人： Bo Curry , Paige Anderson
发明人： Bo Curry , Paige Anderson
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6841 , C12Q2565/501 , C12Q2565/102 , C12Q2533/101
摘要： Methods and compositions for performing array-based CGH assays are provided. In general, the methods involve evaluating copy number of a genomic region by evaluating binding of distinguishably labeled populations of nucleic acids to a first CGH array in which: a) the populations of nucleic acids are labeled by a labeled primer and/or b) binding is assessed relative to binding of dye-swapped populations of nucleic acids to a second CGH array. Kits and systems for use in practicing the subject methods are also provided.
摘要翻译：提供了用于进行基于阵列的CGH测定法的方法和组合物。通常，所述方法包括通过评估核酸的可区分标记的群体与第一CGH阵列的结合来评估基因组区域的拷贝数，其中：a）核酸群体被标记的引物和/或b）结合相对于染色体交换的核酸群体与第二CGH阵列的结合来评估。还提供了用于实践主题方法的套件和系统。

55. 发明授权

US07018794B2 Methods for monitoring multiple gene expression 失效
公开(公告)号：US07018794B2
公开(公告)日：2006-03-28
申请号：US09974300
申请日：2001-10-05
申请人： Randy M. Berka , Ib Groth Clausen , Alexandre Bolotine , Alexei Sorokine , Alla Lapidus
发明人： Randy M. Berka , Ib Groth Clausen , Alexandre Bolotine , Alexei Sorokine , Alla Lapidus
IPC分类号： C12Q1/68 , C07H21/00 , C12N15/09
CPC分类号： C12Q1/6809 , C12Q1/689 , C12Q2600/158 , C12Q2565/501 , C12Q2565/102
摘要： The present invention relates to methods for monitoring differential expression of a plurality of genes in a first Bacillus cell relative to expression of the same genes in one or more second Bacillus cells using microarrays containing Bacillus genomic sequenced tags. The present invention also relates to computer readable media and computer-based systems. The present invention further relates to substrates containing an array of Bacillus licheniformis or Bacillus clausii GSTs.

56. 发明申请

US20050221360A1 Method for purifying microbeads 审中-公开
标题翻译：微珠纯化方法
公开(公告)号：US20050221360A1
公开(公告)日：2005-10-06
申请号：US11088843
申请日：2005-03-25
申请人： Sachiko Okamoto , Junichi Mineno , Kiyozo Asada , Ikunoshin Kato
发明人： Sachiko Okamoto , Junichi Mineno , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/518 , C12Q2565/102 , C12Q2563/125 , C12Q2563/107 , C12Q2525/161 , C12Q2565/1025
摘要： A method for purifying a microbead having an immobilized nucleic acid, a kit for the method, a microbead array which is an array of microbeads each having an immobilized nucleic acid, and a kit for preparing the microbead array.
摘要翻译：用于纯化具有固定化核酸的微珠的方法，该方法的试剂盒，作为各自具有固定化核酸的微珠阵列的微珠阵列和用于制备微珠阵列的试剂盒。

57. 发明授权

US06942973B2 Methods for isolating genes from microorganisms 失效
标题翻译：从微生物分离基因的方法
公开(公告)号：US06942973B2
公开(公告)日：2005-09-13
申请号：US10095975
申请日：2002-03-12
申请人： Debbie S. Yaver , Randy M. Berka
发明人： Debbie S. Yaver , Randy M. Berka
IPC分类号： C12N15/10 , C12N15/30 , C12N15/52 , C12N15/74 , C12Q1/68 , C07H21/04 , C12N9/08 , C12P21/02
CPC分类号： C12Q1/6895 , C12N15/1034 , C12Q1/6809 , C12Q2600/158 , C12Q2565/501 , C12Q2565/102 , C12Q2525/179
摘要： The present invention relates to methods for isolating a gene encoding an enzyme, comprising: (a) adding a mixture of labeled first nucleic acid probes from a microbial strain cultured on medium without the substrate, and labeled second nucleic acid probes from a microbial strain cultured on medium with the substrate, to an array of random nucleic acid fragments of the microbial strain where the labeled nucleic acids hybridize to complementary sequences of the genomic fragments in the array, wherein the first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter; (b) examining the array under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot in the array; and (c) isolating a gene from the microbial strain that encodes an enzyme that degrades the substrate. The present invention also relates to isolated genes obtained by such methods.
摘要翻译：本发明涉及用于分离编码酶的基因的方法，其包括：（a）从培养基上培养的微生物菌株中加入标记的第一核酸探针的混合物，而不含底物，以及来自培养的微生物菌株的标记的第二核酸探针在具有底物的培养基上的微生物菌株的随机核酸片段的阵列，其中标记的核酸与阵列中的基因组片段的互补序列杂交，其中第一核酸探针用第一报道分子标记，核酸探针用第二报告物标记; （b）在条件下检查阵列，其中微生物菌株的基因的相对表达通过阵列中每个斑点的观察杂交报道信号确定; 和（c）从编码降解底物的酶的微生物菌株中分离基因。本发明还涉及通过这些方法获得的分离基因。

58. 发明授权

US06905826B2 Methods and compositions for microarray control 有权
标题翻译：微阵列控制的方法和组成
公开(公告)号：US06905826B2
公开(公告)日：2005-06-14
申请号：US10050188
申请日：2002-01-14
申请人： Tracy L. Ferea , Gary P. Schroth
发明人： Tracy L. Ferea , Gary P. Schroth
IPC分类号： C12Q1/68 , C07H19/00 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6837 , C12Q2565/102 , C12Q2545/107 , C12Q2565/549 , C12Q2565/513 , C12Q2545/101
摘要： This invention relates to assay for detecting or determining the amount of target molecules in a sample. In certain embodiments, the invention relates to nucleic acid arrays and controls used in such arrays.
摘要翻译：本发明涉及用于检测或确定样品中靶分子量的测定法。在某些实施方案中，本发明涉及在这种阵列中使用的核酸阵列和对照。

59. 发明申请

US20030235849A1 Unimolecular segment amplification and sequencing 审中-公开
公开(公告)号：US20030235849A1
公开(公告)日：2003-12-25
申请号：US10413041
申请日：2003-04-10
发明人： Paul M. Lizardi , Michael Caplan
IPC分类号： C12Q001/68 , C12P019/34
CPC分类号： C12Q1/6862 , C12Q1/6804 , C12Q1/6809 , C12Q1/6816 , C12Q1/6844 , C12Q1/6853 , C12Q2563/179 , C12Q2531/125 , C12Q2531/143 , C12Q2537/125 , C12Q2531/119 , C12Q2537/143 , C12Q2539/113 , C12Q2565/102 , C12Q2525/161 , C12Q2563/131 , C12Q2525/307
摘要： Disclosed are compositions and a method for amplification of and multiplex detection of molecules of interest involving rolling circle replication. The method is useful for simultaneously detecting multiple specific nucleic acids in a sample with high specificity and sensitivity. The method also has an inherently low level of background signal. A preferred form of the method consists of an association operation, an amplification operation, and a detection operation. The association operation involves association of one or more specially designed probe molecules, either wholly or partly nucleic acid, to target molecules of interest. This operation associates the probe molecules to a target molecules present in a sample. The amplification operation is rolling circle replication of circular nucleic acid molecules, termed amplification target circles, that are either a part of, or hybridized to, the probe molecules. A single round of amplification using rolling circle replication results in a large amplification of the amplification target circles. Following rolling circle replication, the amplified sequences are detected using combinatorial multicolor coding probes that allow separate, simultaneous, and quantitative detection of multiple different amplified target circles representing multiple different target molecules. Since the amplified product is directly proportional to the amount of target sequence present in a sample, quantitative measurements reliably represent the amount of a target sequence in a sample. Major advantages of this method are that a large number of distinct target molecules can be detected simultaneously, and that differences in the amounts of the various target molecules in a sample can be accurately quantified. It is also advantageous that the DNA replication step is isothermal, and that signals are strictly quantitative because the amplification reaction is linear and is catalyzed by a highly processive enzyme.

60. 发明申请

US20030175719A1 Random array of micro-spheres for the analysis of nucleic acid using enzyme digestion 失效
标题翻译：随机阵列的微球用于分析核酸酶消化
公开(公告)号：US20030175719A1
公开(公告)日：2003-09-18
申请号：US10098642
申请日：2002-03-15
发明人： Tiecheng A. Qiao , Krishnan Chari , Douglas L. Vizard
IPC分类号： C12Q001/68 , C12M001/34
CPC分类号： C12Q1/6837 , B01J2219/00466 , B01J2219/00545 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , C40B40/06 , C40B70/00 , C12Q2565/513 , C12Q2565/102 , C12Q2563/149
摘要： A method of identifying nucleic acid samples comprising: providing a mircoarray including a substrate coated with a composition including a population of nucleic acid probe modified micro-spheres immobilized in a coating containing a gelling agent or a precursor to a gelling agent, wherein a first portion of the micro-spheres is submerged in the gelatin coating and a second portion is exposed above the gelatin coating and is substantially free of gelatin, at least one sub-population of the population micro-spheres containing an optical barcode generated from at least one colorant associated with the micro-spheres and including a nucleic acid probe sequence; contacting the array with a target nucleic acid sequence; and detecting the color barcode of the sub-population of micro-spheres due to the interaction of the probe nucleic acid sequence and the fluorescently/chemiluminescently labeled nucleic acid sample target nucleic acid sequence.
摘要翻译：一种鉴定核酸样品的方法，包括：提供包含底物的微阵列，所述底物涂覆有组合物，所述组合物包含固定在包含胶凝剂或胶凝剂前体的涂层中的核酸探针修饰微球体群体，其中第一部分的微球浸没在明胶涂层中，第二部分暴露在明胶涂层上方并且基本上不含明胶，至少一个群体微球的亚群包含由至少一种着色剂产生的光学条形码与微球相关并包括核酸探针序列; 使阵列与靶核酸序列接触; 并且由于探针核酸序列和荧光/化学发光标记的核酸样品靶核酸序列的相互作用，检测微球子群的彩色条形码。

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