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    • 55. 发明授权
    • Method for measuring cystatin C in human body fluid
    • 人体液体中半胱氨酸蛋白酶抑制剂C的测定方法
    • US08802446B2
    • 2014-08-12
    • US13132790
    • 2009-12-03
    • Shinya NakayamaHiroshi TakahashiYasushi NakamuraTomo Shimizu
    • Shinya NakayamaHiroshi TakahashiYasushi NakamuraTomo Shimizu
    • G01N33/543
    • G01N33/543G01N33/6893G01N2333/8139
    • Provided is a method and reagent for measuring cystatin C in a human body fluid, by contacting a human body fluid with (a) first insoluble carrier particles coated with a first anti-human cystatin C monoclonal antibody and (b) second insoluble carrier particles coated with a second anti-human cystatin C monoclonal antibody, where the first anti-human cystatin C monoclonal antibody has an affinity for cystatin C that is higher than the second anti-human cystatin C monoclonal antibody, the second anti-human cystatin C monoclonal antibody recognizes an epitope of cystatin C that is different from the epitope recognized by the first anti-human cystatin C monoclonal antibody, and the first and second insoluble carrier particles are coated with from 1% to less than 4% by weight of the anti-human cystatin C monoclonal antibodies.
    • 本发明提供一种通过使人体液与(a)涂覆有第一抗人半胱氨酸蛋白酶抑制剂C单克隆抗体的第一不溶性载体颗粒和(b)涂覆有第一抗人半胱氨酸蛋白酶抑制剂C单克隆抗体的第二不溶性载体颗粒接触来测量人体液体中的半胱氨酸蛋白酶抑制剂C的方法和试剂 使用第二抗人半胱氨酸蛋白酶抑制剂C单克隆抗体,其中第一抗人半胱氨酸蛋白酶抑制剂C单克隆抗体对半胱氨酸蛋白酶抑制剂C具有高于第二抗人半胱氨酸蛋白酶抑制剂C单克隆抗体的亲和力,第二抗人半胱氨酸蛋白酶抑制剂C单克隆抗体 识别与由第一抗人半胱氨酸蛋白酶抑制剂C单克隆抗体识别的表位不同的半胱氨酸蛋白酶抑制剂C的表位,并且第一和第二不溶性载体颗粒涂覆有1%至小于4重量%的抗人 半胱氨酸蛋白酶抑制剂C单克隆抗体。