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51. 发明申请

US20100029506A1 Label-Free Monitoring of Excitation-Contraction Coupling and Excitable Cells Using Impedance Based Systems with Millisecond Time Resolution 有权
标题翻译：使用基于阻抗的系统以毫秒时间分辨率对激发 - 收缩耦合和可激励单元进行无标签监控
公开(公告)号：US20100029506A1
公开(公告)日：2010-02-04
申请号：US12435569
申请日：2009-05-05
申请人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： C40B30/06 , C40B60/12
CPC分类号： G01N33/4836
摘要： Systems and methods for improved monitoring of excitation-contraction coupling and excitable cells are provided, which provide millisecond time resolution. The system is capable of continuously monitoring excitation-contraction coupling in a relatively high-throughput manner. The system includes a device for monitoring cell-substrate impedance, an impedance analyzer capable of impedance measurements at millisecond time resolution, electronic circuitry that can engage the device and selectively connect two or more electrode arrays of the device to the impedance analyzer and a software program that controls the electronic circuitry and records and analyzes data obtained from the impedance analyzer.
摘要翻译：提供了用于改善激发 - 收缩耦合和可兴奋电池监测的系统和方法，其提供毫秒时间分辨率。该系统能够以相对高通量的方式连续地监视激励 - 收缩耦合。该系统包括用于监测电池基板阻抗的装置，能够以毫秒时间分辨率进行阻抗测量的阻抗分析仪，可接合该装置并有选择地将该装置的两个或多个电极阵列连接到阻抗分析器的电子电路和一个软件程序控制电子电路并记录和分析从阻抗分析仪获得的数据。

52. 发明授权

US07560269B2 Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays 有权
标题翻译：实时电子细胞感测系统和细胞毒性分析和化合物分析的应用
公开(公告)号：US07560269B2
公开(公告)日：2009-07-14
申请号：US11055639
申请日：2005-02-09
申请人： Xiaobo Wang , Xiao Xu , Yama Abassi
发明人： Xiaobo Wang , Xiao Xu , Yama Abassi
IPC分类号： C12M1/42 , C12M1/34 , C12M3/00 , C12N13/00
CPC分类号： C12Q1/025 , C12Q1/002 , G01N27/02 , G01N33/4836 , G01N33/5014 , G01N33/5438
摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.
摘要翻译：本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件用于控制设备站和阻抗分析仪。在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。该方法可用于测试化合物对细胞的影响，例如细胞毒性测定。还提供了化合物的细胞毒性分析方法。

53. 发明授权

US07470533B2 Impedance based devices and methods for use in assays 有权
标题翻译：基于阻抗的装置和方法用于测定
公开(公告)号：US07470533B2
公开(公告)日：2008-12-30
申请号：US10705447
申请日：2003-11-10
申请人： Xiao Xu , Xiaobo Wang
发明人： Xiao Xu , Xiaobo Wang
IPC分类号： C12M1/42 , C12M3/00 , C12M1/36 , C12M1/38 , C12M1/34
CPC分类号： G01N27/02 , C12M23/12 , C12M41/36 , G01N27/3276 , Y10T29/49117
摘要： A device for detecting cells and/or molecules on an electrode surface is disclosed. The device detects cells and/or molecules through measurement of impedence changes resulting from the cells and/or molecules. A disclosed embodiment of the device includes a substrate having two opposing ends along a longitudinal axis. A plurality of electrode arrays are positioned on the substrate. Each electrode array includes at least two electrodes, and each electrode is separated from at least one adjacent electrode in the electrode array by an expanse of non-conductive material. The electrode has a width at its widest point of more than about 1.5 and less than about 10 times the width of the expanse of non-conductive material. The device also includes electrically conductive traces extending substantially longitudinally to one of the two opposing ends of the substrate without intersecting another trace. Each trace is in electrical communication with at least one of the electrode arrays.
摘要翻译：公开了一种用于检测电极表面上的电池和/或分子的装置。该装置通过测量由细胞和/或分子产生的阻抗变化来检测细胞和/或分子。所述装置的公开实施例包括具有沿着纵向轴线的两个相对端部的基板。多个电极阵列位于基板上。每个电极阵列包括至少两个电极，并且每个电极由电极阵列中的至少一个相邻的电极通过一片非导电材料分开。电极的最宽点处的宽度大于非导电材料的宽度的大约1.5倍且小于约10倍。该器件还包括基本上纵向延伸到衬底的两个相对端之一的导电迹线，而不与另一迹线相交。每个迹线与至少一个电极阵列电连通。

54. 发明授权

US07468255B2 Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology 有权
标题翻译：使用实时微电子细胞感测技术测定天然杀伤剂，细胞毒性T淋巴细胞和嗜中性粒细胞介导的靶细胞杀伤的方法
公开(公告)号：US07468255B2
公开(公告)日：2008-12-23
申请号：US11197994
申请日：2005-08-04
申请人： Xiao Xu , Yama Abassi , Xiaobo Wang
发明人： Xiao Xu , Yama Abassi , Xiaobo Wang
IPC分类号： G01N33/574
CPC分类号： G01N33/5438 , G01N33/5014 , G01N2333/4716
摘要： The present invention includes a method of measuring cytolytic activity including providing a device capable of monitoring cell-substrate impedance operably connected to an impedance analyzer, adding target cells to at least one well of the device, adding effector cells to the at least one well, monitoring impedance of the at least one well and optionally determining a cell index from the impedance, wherein monitoring impedance includes measuring impedance during at least one time point before and at least one time point after adding effector cells, and determining viability of said target cells after adding effector cells by comparing the impedance or optionally the cell index at the at least one time point after adding effector cells to the impedance or optionally the cell index at the at least one time point before adding effector cells.
摘要翻译：本发明包括一种测量溶细胞活性的方法，包括提供能够监测可操作地连接到阻抗分析仪的细胞 - 基底阻抗的装置，将靶细胞添加到装置的至少一个孔中，将效应细胞添加到至少一个孔，监测所述至少一个井的阻抗并且可选地从所述阻抗确定细胞指数，其中监测阻抗包括在加入效应细胞之前和至少一个时间点之前的至少一个时间点内测量阻抗，以及在确定所述靶细胞之后的存活力通过在添加效应细胞之前在至少一个时间点将效应细胞添加到阻抗或任选地在至少一个时间点的细胞指数之后，比较阻抗或任选地在至少一个时间点的细胞指数来添加效应细胞。

55. 发明申请

US20070172939A1 Real time electronic cell sensing system and applications for cell-based assays 有权
标题翻译：实时电子传感系统和基于细胞的测定应用
公开(公告)号：US20070172939A1
公开(公告)日：2007-07-26
申请号：US11725040
申请日：2007-03-15
申请人： Xiao Xu , Yama Abassi , Xiaobo Wang , Jiangbo Gan
发明人： Xiao Xu , Yama Abassi , Xiaobo Wang , Jiangbo Gan
IPC分类号： C12M1/34
CPC分类号： G01N33/6854 , C12M25/08 , C12M41/36 , G01N33/4836 , G01N33/5005 , G01N2500/10
摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.
摘要翻译：本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件用于控制设备站和阻抗分析仪。在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。在一些优选的方面，设计测定法以研究化合物对细胞的影响，例如细胞毒性测定。在其它优选方面，设计这些测定法来研究影响细胞对抗原的IgE介导的反应的化合物。

56. 发明申请

US20060120204A1 Dynamic monitoring of cell adhesion and spreading using the RT-CES system 有权
标题翻译：使用RT-CES系统动态监测细胞粘附和扩散
公开(公告)号：US20060120204A1
公开(公告)日：2006-06-08
申请号：US11235938
申请日：2005-09-27
申请人： Yama Abassi , Josephine Atienza , Xiao Xu , Xiaobo Wang
发明人： Yama Abassi , Josephine Atienza , Xiao Xu , Xiaobo Wang
IPC分类号： G11C8/02
CPC分类号： G01N29/036 , G01N2291/0257
摘要： The present invention includes devices and methods for dynamically monitoring cell adhesion and cell spreading. Cells are added to a microelectronic cell sensor array operably connected to an impedance analyzer. The device also includes a coating including biological molecule or organic compound capable of interacting with the cell. Cell adhesion and cell mobility is determined by detecting changes in impedance and comparing impedance or cell index values between samples.
摘要翻译：本发明包括用于动态监测细胞粘附和细胞扩散的装置和方法。将细胞添加到可操作地连接到阻抗分析器的微电子单元传感器阵列。该装置还包括包含能够与细胞相互作用的生物分子或有机化合物的涂层。通过检测阻抗变化并比较样品之间的阻抗或细胞指数值来确定细胞粘附和细胞迁移率。

57. 发明申请

US20060023559A1 Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology 有权
标题翻译：使用实时微电子细胞感测技术测定天然杀伤剂，细胞毒性T淋巴细胞和嗜中性粒细胞介导的靶细胞杀伤的方法
公开(公告)号：US20060023559A1
公开(公告)日：2006-02-02
申请号：US11197994
申请日：2005-08-04
申请人： Xiao Xu , Yama Abassi , Xiaobo Wang
发明人： Xiao Xu , Yama Abassi , Xiaobo Wang
IPC分类号： G11C8/00
CPC分类号： G01N33/5438 , G01N33/5014 , G01N2333/4716
摘要： The present invention includes a method of measuring cytolytic activity including providing a device capable of monitoring cell-substrate impedance operably connected to an impedance analyzer, adding target cells to at least one well of the device, adding effector cells to the at least one well, monitoring impedance of the at least one well and optionally determining a cell index from the impedance, wherein monitoring impedance includes measuring impedance during at least one time point before and at least one time point after adding effector cells, and determining viability of said target cells after adding effector cells by comparing the impedance or optionally the cell index at the at least one time point after adding effector cells to the impedance or optionally the cell index at the at least one time point before adding effector cells
摘要翻译：本发明包括一种测量溶细胞活性的方法，包括提供能够监测可操作地连接到阻抗分析仪的细胞 - 基底阻抗的装置，将靶细胞添加到装置的至少一个孔中，将效应细胞添加到至少一个孔，监测所述至少一个井的阻抗并且可选地从所述阻抗确定细胞指数，其中监测阻抗包括在加入效应细胞之前和至少一个时间点之前的至少一个时间点内测量阻抗，以及在确定所述靶细胞之后的存活力通过在添加效应细胞之前在至少一个时间点将效应细胞添加到阻抗或任选地在至少一个时间点的细胞指数之后比较阻抗或任选的细胞指数来添加效应细胞，

58. 外观设计

USD1030100S1 Lamp 有权
公开(公告)号：USD1030100S1
公开(公告)日：2024-06-04
申请号：US29836828
申请日：2022-04-29
申请人： Xiaobo Wang , Xudong Shao
设计人： Xiaobo Wang , Xudong Shao
摘要： FIG. 1 is a perspective view of a lamp showing our new design;
FIG. 2 is a front view thereof;
FIG. 3 is a back view thereof;
FIG. 4 is a left view thereof;
FIG. 5 is a right view thereof;
FIG. 6 is a top view thereof;
FIG. 7 is a bottom view thereof;
FIG. 8 is an exploded view thereof; and,
FIG. 9 is a perspective view thereof, shown in an environment of use.
The broken lines shown on the drawings depict portions of the lamp that form no part of the claimed design. The broken lines shown in FIG. 9 illustrate environment and form no part of the claim.

59. 发明授权

US09612234B2 Data analysis of impedance-based cardiomyocyte-beating signals as detected on real-time cell analysis (RTCA) cardio instruments 有权
公开(公告)号：US09612234B2
公开(公告)日：2017-04-04
申请号：US13109809
申请日：2011-05-17
申请人： Nan Li , Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
发明人： Nan Li , Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： C40B30/06 , G01N7/00 , C12Q1/00 , G01N33/50 , G01N33/483
CPC分类号： G01N33/48728 , G01N33/4836 , G01N33/5008 , G01N33/5014 , G01N33/5061 , G01N33/5073 , G01N2800/326 , G01N2800/52
摘要： A method of determining one or more beating parameters for use in cardiomyocyte beating analysis including: providing a cell analysis device including wells, each well including a sensor capable of monitoring beating of cardiomyoctes in millisecond time resolution; adding cardiomyocytes to the wells; monitoring the beating of the cardiomyocytes in millisecond time resolution to obtain a plurality of beating measurements; and calculating one or more beating parameters from the plurality of beating measurements.

60. 发明授权

US09359639B2 Method for the detection of multiple single nucleotide variations or single nucleotide polymorphisms in a single tube 有权
标题翻译：在单个管中检测多个单核苷酸变异或单核苷酸多态性的方法
公开(公告)号：US09359639B2
公开(公告)日：2016-06-07
申请号：US13322664
申请日：2010-05-26
申请人： Qingge Li , Qiuying Huang , Xiaobo Wang
发明人： Qingge Li , Qiuying Huang , Xiaobo Wang
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2525/161 , C12Q2527/107 , C12Q2561/125
摘要： The present invention discloses a method for detecting multiple single nucleotide variations or polymorphisms in a single reaction tube, and the oligonucleotide, the probe, the set of probes, the kit used, as well as the use thereof. Specifically, it relates to a method for identifying the genotype of multiple single nucleotide variation or SNP sites from the melting temperature of a kind of artificial melting temperature tag sequence (AMTS) and the type of fluorescence labels.
摘要翻译：本发明公开了一种用于检测单个反应管中多个单核苷酸变异或多态性的方法，以及寡核苷酸，探针，探针组，所用试剂盒及其用途。具体地说，涉及从一种人工融化温度标签序列（AMTS）的解链温度和荧光标记类型中鉴定多个单核苷酸变异或SNP位点的基因型的方法。

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