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1. 发明申请

US20110117542A1 REAL TIME ELECTRONIC CELL SENSING SYSTEM AND APPLICATIONS FOR CYTOTOXICITY PROFILING AND COMPOUND ASSAYS 有权
标题翻译：实时电子细胞感测系统及其应用于细胞毒性分析和化合物测定
公开(公告)号：US20110117542A1
公开(公告)日：2011-05-19
申请号：US13011725
申请日：2011-01-21
申请人： Yama Abassi , Xiaobo Wang , Xiao Xu
发明人： Yama Abassi , Xiaobo Wang , Xiao Xu
IPC分类号： C12Q1/02 , C12Q1/70 , C12Q1/68
CPC分类号： C12Q1/025 , C12Q1/002 , G01N27/02 , G01N33/4836 , G01N33/5014 , G01N33/5438
摘要： The invention provides methods of investigating a mechanism of action of a compound, which includes providing a device for monitoring cell-substrate impedance; attaching the device to an impedance analyzer; adding cells to two or more wells; adding a test compound to at least one of the wells and providing at least one control well; monitoring impedance of the wells to obtain a series of impedance measurements; plotting impedance measurements to obtain impedance curves and comparing the impedance curves to determine a time frame at which the test compound has a significant effect on cell growth or behavior. Determining the time frame provides information on changes in cell status in response to the test compound, including cell attachment or adhesion status, cell growth or proliferation status, the number of viable or dead cells, cytoskeletal organization or structure, and the number of cells going through apoptosis or necrosis.
摘要翻译：本发明提供了研究化合物作用机理的方法，其包括提供用于监测细胞 - 基底阻抗的装置; 将设备连接到阻抗分析仪; 将细胞添加到两个或更多个孔中; 向至少一个孔中加入测试化合物并提供至少一个对照孔; 监测井的阻抗以获得一系列阻抗测量; 绘制阻抗测量以获得阻抗曲线并比较阻抗曲线以确定测试化合物对细胞生长或行为具有显着影响的时间框架。确定时间框架提供了响应于测试化合物的细胞状态变化的信息，包括细胞附着或粘附状态，细胞生长或增殖状态，活细胞或死细胞的数量，细胞骨架组织或结构以及细胞数目通过凋亡或坏死。

2. 发明申请

US20090048271A1 HETEROCYCLIC COMPOUNDS AND USES AS ANTICANCER AGENTS 有权
标题翻译：杂环化合物和作为反应剂的用途
公开(公告)号：US20090048271A1
公开(公告)日：2009-02-19
申请号：US12175154
申请日：2008-07-17
申请人： Haoyun An , Biao Xi , Yama Abassi , Xiaobo Wang , Xiao Xu
发明人： Haoyun An , Biao Xi , Yama Abassi , Xiaobo Wang , Xiao Xu
IPC分类号： A61K31/519 , A61P35/00 , C07D487/04 , A61K31/429 , C07D513/04
CPC分类号： C07D487/04 , C07D498/04 , C07D513/04
摘要： Novel compounds having a fused bicyclic heteroaromatic ring system substituted with a heteroaryl five-membered ring are disclosed. The compounds inhibit growth of a variety of types of cancer cells, and are thus useful for treating cancer. Efficacy of these compounds is demonstrated with a system for monitoring cell growth/migration, which shows they are potent inhibitors of growth and/or migration of cancer cells. In addition, compounds of the invention were shown to stop growth of tumors in vivo, and to reduce the size of tumors in vivo. Compositions comprising these compounds, and methods to use these compounds and compositions for treatment of cancers, are disclosed.
摘要翻译：公开了具有被杂芳基五元环取代的稠合双环杂芳环系的新型化合物。这些化合物抑制多种类型的癌细胞的生长，因此可用于治疗癌症。通过用于监测细胞生长/迁移的系统来证明这些化合物的功效，这表明它们是癌细胞的生长和/或迁移的有效抑制剂。此外，本发明的化合物被证明可以在体内停止肿瘤生长，并减少体内肿瘤的大小。公开了包含这些化合物的组合物，以及使用这些化合物和用于治疗癌症的组合物的方法。

3. 发明授权

US07732127B2 Dynamic monitoring of cell adhesion and spreading using the RT-CES system 有权
标题翻译：使用RT-CES系统动态监测细胞粘附和扩散
公开(公告)号：US07732127B2
公开(公告)日：2010-06-08
申请号：US11235938
申请日：2005-09-27
申请人： Xiaobo Wang , Yama Abassi , Josephine Atienza , Xiao Xu
发明人： Xiaobo Wang , Yama Abassi , Josephine Atienza , Xiao Xu
IPC分类号： C12Q1/00 , C12N5/00
CPC分类号： G01N29/036 , G01N2291/0257
摘要： The present invention includes devices and methods for dynamically monitoring cell adhesion and cell spreading. Cells are added to a microelectronic cell sensor array operably connected to an impedance analyzer. The device also includes a coating including biological molecule or organic compound capable of interacting with the cell. Cell adhesion and cell mobility is determined by detecting changes in impedance and comparing impedance or cell index values between samples.
摘要翻译：本发明包括用于动态监测细胞粘附和细胞扩散的装置和方法。将细胞添加到可操作地连接到阻抗分析器的微电子单元传感器阵列。该装置还包括包含能够与细胞相互作用的生物分子或有机化合物的涂层。通过检测阻抗变化并比较样品之间的阻抗或细胞指数值来确定细胞粘附和细胞迁移率。

4. 发明授权

US07192752B2 Real time electronic cell sensing systems and applications for cell-based assays 有权
标题翻译：实时电子传感系统和基于细胞的测定的应用
公开(公告)号：US07192752B2
公开(公告)日：2007-03-20
申请号：US10987732
申请日：2004-11-12
申请人： Xiao Xu , Yama Abassi , Xiaobo Wang , Jiangbo Gan
发明人： Xiao Xu , Yama Abassi , Xiaobo Wang , Jiangbo Gan
IPC分类号： C12N13/00
CPC分类号： G01N33/6854 , C12M25/08 , C12M41/36 , G01N33/4836 , G01N33/5005 , G01N2500/10
摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.
摘要翻译：本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件用于控制设备站和阻抗分析仪。在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。在一些优选的方面，设计测定法以研究化合物对细胞的影响，例如细胞毒性测定。在其它优选方面，设计这些测定法来研究影响细胞对抗原的IgE介导的反应的化合物。

5. 发明申请

US20060121446A1 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses 有权
标题翻译：分子进入细胞的基于电穿孔的装置和方法以及细胞反应的动态监测
公开(公告)号：US20060121446A1
公开(公告)日：2006-06-08
申请号：US11286882
申请日：2005-11-23
申请人： Yama Abassi , Xiaobo Wang , Josephine Atienza , Xiao Xu , Junquan Xu
发明人： Yama Abassi , Xiaobo Wang , Josephine Atienza , Xiao Xu , Junquan Xu
IPC分类号： C12Q1/00 , C12N15/86
CPC分类号： C12N15/87 , C12M35/02 , G01N15/12 , G01N33/48728
摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
摘要翻译：本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

6. 发明授权

US08252822B2 Heterocyclic compounds and uses as anticancer agents 有权
标题翻译：杂环化合物和用作抗癌剂
公开(公告)号：US08252822B2
公开(公告)日：2012-08-28
申请号：US12175154
申请日：2008-07-17
申请人： Haoyun An , Biao Xi , Yama Abassi , Xiaobo Wang , Xiao Xu
发明人： Haoyun An , Biao Xi , Yama Abassi , Xiaobo Wang , Xiao Xu
IPC分类号： A61K31/429 , C07D513/04
CPC分类号： C07D487/04 , C07D498/04 , C07D513/04
摘要： Novel compounds having a fused bicyclic heteroaromatic ring system substituted with a heteroaryl five-membered ring are disclosed. The compounds inhibit growth of a variety of types of cancer cells, and are thus useful for treating cancer. Efficacy of these compounds is demonstrated with a system for monitoring cell growth/migration, which shows they are potent inhibitors of growth and/or migration of cancer cells. In addition, compounds of the invention were shown to stop growth of tumors in vivo, and to reduce the size of tumors in vivo. Compositions comprising these compounds, and methods to use these compounds and compositions for treatment of cancers, are disclosed.
摘要翻译：公开了具有被杂芳基五元环取代的稠合双环杂芳环系的新型化合物。这些化合物抑制多种类型的癌细胞的生长，因此可用于治疗癌症。通过用于监测细胞生长/迁移的系统来证明这些化合物的功效，这表明它们是癌细胞的生长和/或迁移的有效抑制剂。此外，本发明的化合物被证明可以在体内停止肿瘤生长，并减少体内肿瘤的大小。公开了包含这些化合物的组合物，以及使用这些化合物和用于治疗癌症的组合物的方法。

7. 发明申请

US20050213374A1 Real time electronic cell sensing system and applications for cytoxicity profiling and compound assays 有权
标题翻译：实时电子细胞感测系统和细胞毒性分析和化合物分析的应用
公开(公告)号：US20050213374A1
公开(公告)日：2005-09-29
申请号：US11055639
申请日：2005-02-09
申请人： Xiao Xu , Yama Abassi , Xiaobo Wang
发明人： Xiao Xu , Yama Abassi , Xiaobo Wang
IPC分类号： G11C11/00
CPC分类号： C12Q1/025 , C12Q1/002 , G01N27/02 , G01N33/4836 , G01N33/5014 , G01N33/5438
摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.
摘要翻译：本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件用于控制设备站和阻抗分析仪。在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。该方法可用于测试化合物对细胞的影响，例如细胞毒性测定。还提供了化合物的细胞毒性分析方法。

8. 发明申请

US20050153425A1 Real time electronic cell sensing systems and applications for cell-based assays 有权
标题翻译：实时电子传感系统和基于细胞的测定的应用
公开(公告)号：US20050153425A1
公开(公告)日：2005-07-14
申请号：US10987732
申请日：2004-11-12
申请人： Xiao Xu , Yama Abassi , Xiaobo Wang , Jiangbo Gan
发明人： Xiao Xu , Yama Abassi , Xiaobo Wang , Jiangbo Gan
IPC分类号： C12M1/34 , C12M1/42 , C12N20060101 , G01N33/487 , H01M4/50
CPC分类号： G01N33/6854 , C12M25/08 , C12M41/36 , G01N33/4836 , G01N33/5005 , G01N2500/10
摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.
摘要翻译：本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件用于控制设备站和阻抗分析仪。在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。在一些优选的方面，设计测定法以研究化合物对细胞的影响，例如细胞毒性测定。在其它优选方面，设计这些测定法来研究影响细胞对抗原的IgE介导的反应的化合物。

9. 发明授权

US07560269B2 Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays 有权
标题翻译：实时电子细胞感测系统和细胞毒性分析和化合物分析的应用
公开(公告)号：US07560269B2
公开(公告)日：2009-07-14
申请号：US11055639
申请日：2005-02-09
申请人： Xiaobo Wang , Xiao Xu , Yama Abassi
发明人： Xiaobo Wang , Xiao Xu , Yama Abassi
IPC分类号： C12M1/42 , C12M1/34 , C12M3/00 , C12N13/00
CPC分类号： C12Q1/025 , C12Q1/002 , G01N27/02 , G01N33/4836 , G01N33/5014 , G01N33/5438
摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.
摘要翻译：本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件用于控制设备站和阻抗分析仪。在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。该方法可用于测试化合物对细胞的影响，例如细胞毒性测定。还提供了化合物的细胞毒性分析方法。

10. 发明授权

US07468255B2 Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology 有权
标题翻译：使用实时微电子细胞感测技术测定天然杀伤剂，细胞毒性T淋巴细胞和嗜中性粒细胞介导的靶细胞杀伤的方法
公开(公告)号：US07468255B2
公开(公告)日：2008-12-23
申请号：US11197994
申请日：2005-08-04
申请人： Xiao Xu , Yama Abassi , Xiaobo Wang
发明人： Xiao Xu , Yama Abassi , Xiaobo Wang
IPC分类号： G01N33/574
CPC分类号： G01N33/5438 , G01N33/5014 , G01N2333/4716
摘要： The present invention includes a method of measuring cytolytic activity including providing a device capable of monitoring cell-substrate impedance operably connected to an impedance analyzer, adding target cells to at least one well of the device, adding effector cells to the at least one well, monitoring impedance of the at least one well and optionally determining a cell index from the impedance, wherein monitoring impedance includes measuring impedance during at least one time point before and at least one time point after adding effector cells, and determining viability of said target cells after adding effector cells by comparing the impedance or optionally the cell index at the at least one time point after adding effector cells to the impedance or optionally the cell index at the at least one time point before adding effector cells.
摘要翻译：本发明包括一种测量溶细胞活性的方法，包括提供能够监测可操作地连接到阻抗分析仪的细胞 - 基底阻抗的装置，将靶细胞添加到装置的至少一个孔中，将效应细胞添加到至少一个孔，监测所述至少一个井的阻抗并且可选地从所述阻抗确定细胞指数，其中监测阻抗包括在加入效应细胞之前和至少一个时间点之前的至少一个时间点内测量阻抗，以及在确定所述靶细胞之后的存活力通过在添加效应细胞之前在至少一个时间点将效应细胞添加到阻抗或任选地在至少一个时间点的细胞指数之后，比较阻抗或任选地在至少一个时间点的细胞指数来添加效应细胞。

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