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51. 发明授权

US06387620B1 Transcription-free selex 失效
标题翻译：无转录检查
公开(公告)号：US06387620B1
公开(公告)日：2002-05-14
申请号：US09362578
申请日：1999-07-28
申请人： Jonathan Drew Smith , Larry Gold
发明人： Jonathan Drew Smith , Larry Gold
IPC分类号： C12Q168
CPC分类号： C12Q1/6811 , C12Q2537/155 , C12Q2531/113 , C12Q2521/501
摘要： Methods are provided for the production of nucleic acid ligands against target molecules using a procedure known as Transcription-free Systematic Evolution of Ligands by EXponential enrichment (Transcription-free SELEX). The Transcription-free SELEX method assembles nucleic acid ligands from fragments of synthetic nucleic acids by annealing those fragments to a complementary template, and then ligating the fragments together.
摘要翻译：提供了使用已知为通过扩增浓缩（无转录的SELEX）的配体的无转录系统进化的方法生产针对靶分子的核酸配体的方法。无转录的SELEX方法通过将这些片段退火成互补模板，然后将片段连接在一起，将合成核酸片段的核酸配体组装起来。

52. 发明授权

US06261783B1 Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction 有权
标题翻译：通过核酸配体 - 配体信标相互作用均匀检测靶
公开(公告)号：US06261783B1
公开(公告)日：2001-07-17
申请号：US09581326
申请日：2000-08-11
申请人： Sumedha Jayasena , Larry Gold
发明人： Sumedha Jayasena , Larry Gold
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C12Q1/6818 , C12Q1/682 , Y10T436/143333 , C12Q2561/12 , C12Q2537/101
摘要： A method for detecting a target molecule in a test mixture suspected of containing said target molecule is described. The nucleic acid ligand capable of binding to the target molecule has a first sequence A and a second sequence B, which are partially complementary sequences that form an imperfect intramolecular duplex, which unwinds upon the binding of the target to the nucleic acid ligand. Sequences A and B are able to participate in extramolecular hybridization reactions only when the duplex is unwound. Three different cascade nucleic acids contain a first sequence and a second sequence, which are partially complementary sequences. At least one sequence is exactly complementary to A or B. The second sequence may be complementary to A or B, or may be a third sequence C, or its complement. The test mixture suspected of containing the target molecule is contacted with the nucleic acid ligand, causing the duplex of the nucleic acid ligand to unwind such that sequences A and B become available for extramolecular hybridization. This mixture is contacted with the first, second, and third cascade nucleic acids so that the unpaired A and B sequences triggers a cascade of intermolecular hybridization involving the cascade nucleic acids in which intermolecular hybridization takes place between A and its complement, B and its complement, and between C its complement, leading to the formation of a multimolecular hybridization complex. The presence of the multimolecular hybridization complex is then detected.
摘要翻译：描述了一种用于检测怀疑含有所述靶分子的试验混合物中的靶分子的方法。能够结合靶分子的核酸配体具有第一序列A和第二序列B，它们是形成不完全分子内双链体的部分互补序列，其解离靶核酸与核酸配体的结合。序列A和B只有当双链体解卷时才能参与分子内杂交反应。三种不同的级联核酸含有第一序列和第二序列，其是部分互补序列。至少一个序列与A或B完全互补。第二个序列可以与A或B互补，或者可以是第三个序列C或其补体。怀疑含有靶分子的试验混合物与核酸配体接触，导致核酸配体的双链体解开，使得序列A和B可用于分子内杂交。该混合物与第一级，第二级和第三级联核酸接触，使得不成对的A和B序列引发级联的分子间杂交，其涉及级联核酸，其中分子间杂交发生在A与其补体之间，B及其补体，和C之间的补体，导致形成多分子杂交复合物。然后检测多分子杂交复合物的存在。

53. 发明授权

US06232071B1 Tenascin-C nucleic acid ligands 失效
标题翻译：腱生蛋白-C核酸配体
公开(公告)号：US06232071B1
公开(公告)日：2001-05-15
申请号：US09364902
申请日：1999-07-29
申请人： Brian Hicke , Stephen Warren , David Parma , Larry Gold
发明人： Brian Hicke , Stephen Warren , David Parma , Larry Gold
IPC分类号： C12Q168
CPC分类号： C12N15/115 , A61K38/00 , A61K47/54 , A61K47/547 , A61K47/549 , A61K2123/00 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C12N9/1276 , C12N15/1048 , C12N2310/13 , C12N2310/317 , C12N2310/3183 , C12N2310/321 , C12N2310/322 , C12N2310/3517 , C12N2310/53 , C12Q1/37 , C12Q1/6883 , C12Q2541/101 , C12Q2600/158 , C40B40/00 , F02B2075/027 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/76 , G01N2333/16 , G01N2333/163 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96455 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976 , C12N2310/3521
摘要： Methods are described for the identification and preparation of nucleic acid ligands to tenascin-C. Included in the invention are specific RNA ligands to tenascin-C identified by the SELEX method. Further included in the invention are methods for detecting the presence of a disease condition in a biological tissue in which tenascin-C is expressed.
摘要翻译：描述了用于鉴定和制备腱生蛋白-C的核酸配体的方法。包括在本发明中的是通过SELEX方法鉴定的腱生蛋白-C的特异性RNA配体。本发明还包括用于检测生物组织中存在腱生蛋白-C的疾病状况的方法。

54. 发明授权

US06194550B1 Systematic polypeptide evolution by reverse translation 失效
标题翻译：通过反向翻译进行系统性多肽演化
公开(公告)号：US06194550B1
公开(公告)日：2001-02-27
申请号：US09197649
申请日：1998-11-23
申请人： Larry Gold , Craig Tuerk , David Pribnow , Jonathan Drew Smith
发明人： Larry Gold , Craig Tuerk , David Pribnow , Jonathan Drew Smith
IPC分类号： C12N1511
CPC分类号： C12N15/1041 , C12Q1/6806 , G01N33/68
摘要： A method for preparing polypeptide ligands of target molecules wherein candidate mixtures comprised of ribosome complexes or mRNA•polypeptide copolymers are partitioned relative to their affinity to the target and amplified to create a new candidate mixture enriched in ribosome complexes or mRNA•polypeptide copolymers with an affinity to the target.
摘要翻译：制备靶分子的多肽配体的方法，其中由核糖体复合物或mRNA多肽共聚物组成的候选混合物相对于它们对靶的亲和力被分配，并被扩增，以产生富含核糖体复合物或具有亲和力的mRNA多肽共聚物的新候选混合物到目标。

55. 发明授权

US06177555B1 Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction 有权
标题翻译：通过核酸配体 - 配体信标相互作用均匀检测靶
公开(公告)号：US06177555B1
公开(公告)日：2001-01-23
申请号：US09447863
申请日：1999-11-23
申请人： Sumedha Jayasena , Larry Gold
发明人： Sumedha Jayasena , Larry Gold
IPC分类号： C07H2104
CPC分类号： C07H21/00 , C12Q1/6818 , C12Q1/682 , C12Q2561/12 , C12Q2537/101
摘要： The presence of a target in a test mixture or the concentration of such target can be determined by a method relying on fluorescence emission measurement. Such method utilizes a nucleic acid ligand to the target and a ligand beacon comprising: i) a nucleic acid sequence complimentary to at least a portion of said nucleic acid ligand, ii) a fluorescent group, and iii) a fluorescence-modifying group. The emission profile of the ligand beacon is altered based on the presence and/or concentration change of the target in the test mixture.
摘要翻译：目标在测试混合物中的存在或这种靶标的浓度可以通过依赖于荧光发射测量的方法来确定。这种方法利用靶向的核酸配体和配体信标，其包括：i）与所述核酸配体的至少一部分互补的核酸序列，ii）荧光基团，和iii）荧光修饰基团。基于测试混合物中靶的存在和/或浓度变化，改变配体信标的发射谱。

56. 发明授权

US06140490A High affinity nucleic acid ligands of complement system proteins 失效
标题翻译：补体系蛋白的高亲和力核酸配体
公开(公告)号：US06140490A
公开(公告)日：2000-10-31
申请号：US23228
申请日：1998-02-12
申请人： Gregory Biesecker , Larry Gold
发明人： Gregory Biesecker , Larry Gold
IPC分类号： C12N15/09 , A61K38/00 , A61K48/00 , C07B61/00 , C07H21/00 , C07H21/04 , C07K14/47 , C12N15/115 , C12Q1/68 , C12P19/34
CPC分类号： C07K14/472 , A61K45/06 , C07H21/00 , C12N15/1048 , C12N15/115 , A61K38/00 , C12N2310/151 , C12N2310/315 , C12N2310/322 , C40B40/00
摘要： Methods are described for the identification and preparation of high-affinity Nucleic Acid Ligands to Complement System Proteins. Methods are described for the identification and preparation of high affinity Nucleic Acid Ligands to Complement System Proteins C1q, C3 and C5. Included in the invention arc specific RNA ligands to C1q, C3 and C5 identified by the SELEX method.
摘要翻译：描述了用于鉴定和制备补体系统蛋白质的高亲和力核酸配体的方法。描述了用于鉴定和制备补充系统蛋白C1q，C3和C5的高亲和力核酸配体的方法。本发明包括通过SELEX方法鉴定的C1q，C3和C5的特异性RNA配体。

57. 发明授权

US6030776A Parallel SELEX 失效
标题翻译：平行SELEX
公开(公告)号：US6030776A
公开(公告)日：2000-02-29
申请号：US793398
申请日：1997-02-24
申请人： Bruce Eaton , Larry Gold
发明人： Bruce Eaton , Larry Gold
IPC分类号： C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/00 , C12N9/12 , C12N15/10 , C12N15/115 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12N15/115 , A61K47/48076 , A61K47/48092 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C12N15/1048 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/76 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C40B40/00 , F02B2075/027 , G01N2333/163 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： This invention discloses a method for coevolving products from two or more reactants, along with the nucleic acid that can facilitate the reaction for making the products. The invention further discloses the products and facilitating nucleic acids produced by said method.
摘要翻译： PCT No.PCT / US95 / 11982 Sec。 371日期1997年2月24日 102（e）1997年2月24日PCT PCT 1995年9月19日PCT公布。出版物WO96 / 09316 日期1996年3月28日本发明公开了一种用于将来自两种或更多种反应物的产物共同成型的方法以及可促进制备产物的反应的核酸。本发明还公开了通过所述方法生产的产物和促进核酸。

58. 发明授权

US6020130A Nucleic acid ligands that bind to and inhibit DNA polymerases 失效
标题翻译：结合并抑制DNA聚合酶的核酸配体
公开(公告)号：US6020130A
公开(公告)日：2000-02-01
申请号：US945734
申请日：1997-10-28
申请人： Larry Gold , Sumedha Javasena
发明人： Larry Gold , Sumedha Javasena
IPC分类号： C12N15/09 , C12Q1/68 , C12P1/68 , C07H21/02 , C07H21/04 , C12Q19/34
CPC分类号： C12Q1/6811 , C12N15/115 , C12Q1/6848 , C12Y207/07007 , C12N2310/16 , G01N2333/96433 , Y10S435/81
摘要： This invention discloses high-affinity oligonucleotide ligands to the thermostable Taq polymerase and Tth polymerase. Specifically, this invention discloses DNA ligands having the ability to bind to the Taq and Tth polymerases and the methods for obtaining such ligands. The ligands are capable of inhibiting polymerases at ambient temperatures.
摘要翻译： PCT No.PCT / US96 / 09451 Sec。 371日期1997年10月28日第 102（e）1997年10月28日PCT PCT 1996年6月5日PCT公布。出版物WO96 / 41010 日期1996年12月19日本发明公开了耐热Taq聚合酶和Tth聚合酶的高亲和力寡核苷酸配体。具体地，本发明公开了具有结合Taq和Tth聚合酶的能力的DNA配体和获得这种配体的方法。配体能够在环境温度下抑制聚合酶。

59. 发明授权

US5849479A High-affinity oligonucleotide ligands to vascular endothelial growth factor (VEGF) 失效
公开(公告)号：US5849479A
公开(公告)日：1998-12-15
申请号：US233012
申请日：1994-04-25
申请人： Nebojsa Janjic , Larry Gold
发明人： Nebojsa Janjic , Larry Gold
IPC分类号： A61K38/00 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K7/22 , C07K14/00 , C07K14/16 , C07K14/72 , C12N9/12 , C12N15/10 , C12N15/115 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： A61K47/48092 , A61K47/48076 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C07K14/005 , C07K14/72 , C07K7/22 , C12N15/1048 , C12N15/115 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/6842 , G01N33/76 , A61K38/00 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C12N2740/16222 , C40B40/00 , F02B2075/027 , G01N2333/16 , G01N2333/163 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/918 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： Methods are described for the identification and preparation of nucleic acid ligand ligands to vascular endothelial growth factor (VEGF). Included in the invention are specific RNA ligands to VEGF identified by the SELEX method.

60. 发明授权

US5843701A Systematic polypeptide evolution by reverse translation 失效
标题翻译：通过反向翻译进行系统性多肽演化
公开(公告)号：US5843701A
公开(公告)日：1998-12-01
申请号：US829461
申请日：1992-01-31
申请人： Larry Gold , Craig Tuerk , David Pribnow , Jonathan Drew Smith
发明人： Larry Gold , Craig Tuerk , David Pribnow , Jonathan Drew Smith
IPC分类号： C12N15/10 , C12Q1/68 , G01N33/68 , C12P21/02 , C12N15/00
CPC分类号： C12N15/1041 , G01N33/68 , C12Q1/6806
摘要： A method for preparing polypeptide ligands of target molecules wherein candidate mixtures comprised of ribosome complexes or mRNA.multidot.polypeptide copolymers are partitioned relative to their affinity to the target and amplified to create a new candidate mixture enriched in ribosome complexes or mRNA.multidot.polypeptide copolymers with an affinity to the target.
摘要翻译：制备靶分子的多肽配体的方法，其中由核糖体复合物或mRNA多聚体共聚物组成的候选混合物相对于其对靶的亲和力被分配，并被扩增，以产生富含核糖体复合物或mRNAx多肽共聚物的新候选混合物，其与靶标具有亲和力。

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