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51. 发明授权

US08318450B2 Optically-detectable enzyme substrates and their method of use 有权
标题翻译：光学上可检测的酶底物及其使用方法
公开(公告)号：US08318450B2
公开(公告)日：2012-11-27
申请号：US13166627
申请日：2011-06-22
申请人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
发明人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
IPC分类号： C12Q1/34
CPC分类号： C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
摘要翻译：本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。除了化合物之外，公开了用于分析样品存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。

52. 发明授权

US08124368B2 Fluorogenic protein kinase substrates 有权
标题翻译：荧光蛋白激酶底物
公开(公告)号：US08124368B2
公开(公告)日：2012-02-28
申请号：US11774554
申请日：2007-07-06
申请人： Kyle Gee
发明人： Kyle Gee
IPC分类号： C12Q1/48 , C12Q1/50 , C12M1/34 , C12N9/48 , G01N27/327 , C12Q1/25 , C12Q1/00 , C12M1/00 , C12N9/10 , G01N27/411
CPC分类号： C12Q1/485 , G01N33/573
摘要： The present invention relates to kinase sensors comprising a metal chelator and a fluorophore, where the chelator comprises a quinoline group and where the fluorophore is conjugated to the chelator. The invention also relates to methods of using these kinase sensors as well as kits comprising the kinase sensors.
摘要翻译：本发明涉及包含金属螯合剂和荧光团的激酶传感器，其中螯合剂包含喹啉基团，其中荧光团与螯合剂结合。本发明还涉及使用这些激酶传感器的方法以及包含激酶传感器的试剂盒。

53. 发明申请

US20110045503A1 The Use of Novel Coumarins as Glutathione and Thiol Labels 有权
标题翻译：使用新型香豆素作为谷胱甘肽和硫醇标签
公开(公告)号：US20110045503A1
公开(公告)日：2011-02-24
申请号：US12666256
申请日：2008-07-24
申请人： Kyle Gee , Michael Janes , Iain Johnson , Hee Chol Kang
发明人： Kyle Gee , Michael Janes , Iain Johnson , Hee Chol Kang
IPC分类号： G01N33/52 , C07D471/06
CPC分类号： G01N33/52 , C07D491/06 , C07D491/16 , C07F9/5442 , C07K5/0215 , G01N33/582 , G01N2333/4701 , Y10T436/17 , Y10T436/182
摘要： Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.
摘要翻译：描述了与结合硫醇化合物的亲电子反应性基团取代的荧光喹诺酮香豆素化合物。该化合物可用于检测活细胞中氧化应激和相关过程。

54. 发明申请

US20100291547A1 FLUOROGENIC HYDRAZINE-SUBSTITUTED COMPOUNDS 有权
标题翻译：氟化氢取代的化合物
公开(公告)号：US20100291547A1
公开(公告)日：2010-11-18
申请号：US12864038
申请日：2009-01-23
申请人： Aimei Chen , Kyle Gee , Hee Chol Kang
发明人： Aimei Chen , Kyle Gee , Hee Chol Kang
IPC分类号： C12Q1/02 , C07D311/82 , C07F9/54 , C07K14/00 , C07H19/04 , C07H21/00 , G01N21/76 , G01N33/50 , G01N33/68 , C12Q1/70 , C12Q1/68
CPC分类号： G01N33/64 , C07D311/82 , C07D471/06 , C07F5/022 , C07F9/5442 , C07F9/65522 , C09B11/24 , C09K11/06 , C09K2211/10 , C09K2211/1007 , C09K2211/1014 , C09K2211/1022 , C09K2211/1033 , C09K2211/1055 , C09K2211/1088 , G01N21/6428 , G01N21/6486 , G01N33/50 , G01N33/52 , G01N2021/6439 , Y10T436/143333
摘要： The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.
摘要翻译：本公开涉及能够检测含有醛和酮基的分析物的荧光席夫碱形成染料。染料含有亲核肼基附属物，并能够原位结合和检测分析物。

55. 发明申请

US20100009381A1 Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US20100009381A1
公开(公告)日：2010-01-14
申请号：US12500854
申请日：2009-07-10
申请人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
发明人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
IPC分类号： G01N33/53
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

56. 发明申请

US20080009026A1 FLUOROGENIC PROTEIN KINASE SUBSTRATES 有权
标题翻译：荧光蛋白激酶基质
公开(公告)号：US20080009026A1
公开(公告)日：2008-01-10
申请号：US11774554
申请日：2007-07-06
申请人： Kyle Gee
发明人： Kyle Gee
IPC分类号： C12Q1/48
CPC分类号： C12Q1/485 , G01N33/573
摘要： The present invention relates to kinase sensors comprising a metal chelator and a fluorophore, where the chelator comprises a quinoline group and where the fluorophore is conjugated to the chelator. The invention also relates to methods of using these kinase sensors as well as kits comprising the kinase sensors.
摘要翻译：本发明涉及包含金属螯合剂和荧光团的激酶传感器，其中螯合剂包含喹啉基团，其中荧光团与螯合剂结合。本发明还涉及使用这些激酶传感器的方法以及包含激酶传感器的试剂盒。

57. 发明申请

US20070093652A1 DERIVATIVES OF 1,2-DIHYDRO-7-HYDROXYQUINOLINES CONTAINING FUSED RINGS 有权
标题翻译：含有熔融环的1,2-二羟基-7-羟基喹啉的衍生物
公开(公告)号：US20070093652A1
公开(公告)日：2007-04-26
申请号：US11560579
申请日：2006-11-16
申请人： Zhenjun Diwu , Jixiang Liu , Kyle Gee
发明人： Zhenjun Diwu , Jixiang Liu , Kyle Gee
IPC分类号： C07K14/47 , C07H21/04 , C07D491/14
CPC分类号： G01N33/582 , C07D471/04 , C07D491/04 , C07D491/044 , C07D491/14 , C07D491/147 , C07D491/22 , C07D495/04 , C07D495/14 , C07D495/22 , C07D498/14 , C12Q1/6816 , Y10S436/80 , Y10T436/141111 , Y10T436/143333 , Y10T436/145555 , C12Q2563/107
摘要： The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.
摘要翻译：本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。本发明的许多染料是有用的荧光染料。本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其他分析物中的用途。

58. 发明申请

US20060160068A1 Competitive immunoassay 有权
标题翻译：竞争性免疫测定
公开(公告)号：US20060160068A1
公开(公告)日：2006-07-20
申请号：US10943463
申请日：2004-09-17
申请人： Joseph Beechem , Kyle Gee , David Hagen , Iain Johnson , Hee-Chol Kang , Christina Pastula
发明人： Joseph Beechem , Kyle Gee , David Hagen , Iain Johnson , Hee-Chol Kang , Christina Pastula
IPC分类号： C12Q1/70 , C12Q1/68 , C07K16/46 , C07F5/02 , C07D311/88
CPC分类号： G01N33/542 , C07F9/12 , C07F9/65318 , C07F9/65522 , C07F9/6596 , C07K16/46 , Y10S435/968 , Y10S436/80 , Y10S530/807 , Y10T436/13
摘要： The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex may additionally comprise a labeling protein non-covalently bonded to the antibody to form a ternary complex wherein the labeling protein comprises a monovalent antibody fragment or a non-antibody protein that is covalently bonded to a label moiety. The reporter molecule is either quenched by the ligand-binding antibody or by the label moiety of the labeling protein, depending on the reporter molecule and the ligand-binding antibody, wherein the amount of quenching is directly related to the amount of ligand present in the sample. Alternatively, the ligand analog is fluorogenic wherein the ligand analog is essentially non-fluorescent in solution but when bound by the ligand-binding antibody the detectable signal increases. In this instance a decrease in signal, as opposed to the relieving of quenching, is measured for the presence of a target ligand.
摘要翻译：本发明提供配体检测试剂，配体类似物和用于测定样品中配体存在的方法。配体检测试剂包含配体结合抗体和配体类似物以形成抗体 - 配体类似物复合物，其中配体类似物共价键合到报告分子。该复合物还可以包含与抗体非共价键合以形成三元复合物的标记蛋白质，其中标记蛋白质包含共价键合到标记部分的单价抗体片段或非抗体蛋白质。取决于报道分子和配体结合抗体，报告分子被配体结合抗体或标记蛋白的标记部分淬灭，其中淬灭量与存在于样品。或者，配体类似物是荧光的，其中配体类似物在溶液中基本上是非荧光的，但当被配体结合抗体结合时，可检测信号增加。在这种情况下，与目标配体的存在相比，测量了与消除淬灭相反的信号降低。

59. 发明申请

US20060141554A1 Site-specific labeling of affinity tags in fusion proteins 审中-公开
公开(公告)号：US20060141554A1
公开(公告)日：2006-06-29
申请号：US10966536
申请日：2004-10-14
申请人： Kyle Gee , Courtenay Hart , Richard Haugland , Wayne Patton , Scott Whitney
发明人： Kyle Gee , Courtenay Hart , Richard Haugland , Wayne Patton , Scott Whitney
IPC分类号： G01N33/48 , C07F15/04
CPC分类号： C07D263/62 , A61K49/0021 , A61K49/0039 , A61K49/0041 , A61K49/0052 , C07D311/12 , C07D311/16 , C07D311/18 , C07D405/14 , C07F5/022 , G01N1/30 , G01N33/533 , G01N33/582
摘要： The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

60. 发明申请

US20050227309A1 Optically-detectable enzyme substrates and their method of use 审中-公开
标题翻译：光学上可检测的酶底物及其使用方法
公开(公告)号：US20050227309A1
公开(公告)日：2005-10-13
申请号：US11040924
申请日：2005-01-21
申请人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , I. Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
发明人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , I. Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
IPC分类号： C07D487/08 , C07D499/00 , C07D501/00 , C07D501/14 , C07D503/00 , C07D519/00 , C07F5/02 , C09B1/00 , C12Q1/18 , C12Q1/34
CPC分类号： C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
摘要翻译：本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。除了化合物之外，公开了用于分析样品中存在β-内酰胺酶的方法，例如作为包括编码β-内酰胺酶的序列的核酸序列的表达的指示剂。公开了包括公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-内酰胺酶或在测定中使用组分的说明书。

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