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51. 发明授权

US08796510B2 Polynucleotides and polypeptides in plants 有权
标题翻译：植物中的多核苷酸和多肽
公开(公告)号：US08796510B2
公开(公告)日：2014-08-05
申请号：US13367257
申请日：2012-02-06
申请人： Jacqueline E. Heard , Robert A. Creelman , Oliver J. Ratcliffe , T. Lynne Reuber , James S. Keddie
发明人： Jacqueline E. Heard , Robert A. Creelman , Oliver J. Ratcliffe , T. Lynne Reuber , James S. Keddie
IPC分类号： A01H5/00 , A01H5/10 , A01H1/00 , C12N15/82 , C07K14/415
CPC分类号： C12N15/8201 , C07K14/415 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8263 , C12N15/8266 , C12N15/8267 , C12N15/827 , C12N15/8271 , C12N15/8273 , C12N15/8275 , C12N15/8279 , C12N15/8282 , C12N15/8291 , C12N15/8293 , Y02A40/146
摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
摘要翻译：本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同系物，以及使用多核苷酸和多肽产生与参照植物相比具有有利特性的转基因植物的方法。与这些多核苷酸和多肽相关的序列信息也可以用于生物信息学检索方法，并且也被公开。

52. 发明申请

US20100071086A1 POLYSOME-MEDIATED CELL TYPE-, TISSUE TYPE- OR CONDITION-ENHANCED TRANSCRIPT PROFILING 审中-公开
标题翻译：多聚体介质细胞类型，组织类型或状态增强转录物
公开(公告)号：US20100071086A1
公开(公告)日：2010-03-18
申请号：US12557449
申请日：2009-09-10
申请人： Peter P. Repetti , Oliver J. Ratcliffe , Luc J. Adam , T. Lynne Reuber , Hans E. Holtan
发明人： Peter P. Repetti , Oliver J. Ratcliffe , Luc J. Adam , T. Lynne Reuber , Hans E. Holtan
IPC分类号： C12N15/82 , A01H5/00 , C12N5/04 , C12N15/74
CPC分类号： C12N15/8216
摘要： In this invention, a method is described that allows for the efficient creation and identification of validated biological materials that greatly enhance the ability to perform polysome-mediated RNA profiling, such as constitutive, cell type-, tissue type-, or condition-enhanced RNA profiling. The method relies on the use of a tri-partite plant binary expression vector comprised of the following components: a) a DNA promoter element that drives expression of a sequence specific transcription activator protein such as a LexA:Gal4 fusion protein in a unique desired pattern, b) a DNA promoter element comprising a target site for the transcriptional activator protein, such as opLexA, fused to a nucleotide encoding an epitope tagged ribosomal component protein and c) a DNA promoter element comprising a target site for the transcriptional activator protein, such as opLexA, fused to a nucleotide encoding an in vivo reporter protein. By visualization of the co-regulated reporter, this method allows for in planta confirmation that the promoter element is driving expression, such as constitutive, cell type-, tissue type-, or condition-enhanced expression, of the tagged ribosomal protein in the desired cell or tissue types.
摘要翻译：在本发明中，描述了允许有效创建和鉴定验证的生物材料的方法，其极大地增强了进行多聚体介导的RNA分析的能力，例如组成型，细胞类型，组织型或条件增强型RNA 剖析。该方法依赖于使用由以下组分组成的三分子植物二元表达载体：a）以独特的期望模式驱动序列特异性转录激活蛋白如LexA：Gal4融合蛋白的表达的DNA启动子元件 b）包含与编码表位标记的核糖体组分蛋白的核苷酸融合的转录激活蛋白的靶位点如opLexA的DNA启动子元件，和c）包含转录激活蛋白的靶位点的DNA启动子元件，例如作为opLexA，与编码体内报道蛋白的核苷酸融合。通过共同调节的报告者的可视化，这种方法允许在植物中确认启动子元件是驱动所需标记的核糖体蛋白质的组成型，细胞类型，组织型或条件增强表达的表达细胞或组织类型。

53. 发明申请

US20120131691A1 LIGHT-REGULATED PROMOTERS 审中-公开
标题翻译：光调节促销
公开(公告)号：US20120131691A1
公开(公告)日：2012-05-24
申请号：US13322136
申请日：2010-05-17
申请人： Peter P. Repetti , Rajnish Khanna , Hans E. Holtan , T. Lynne Reuber , Oliver J. Ratcliffe
发明人： Peter P. Repetti , Rajnish Khanna , Hans E. Holtan , T. Lynne Reuber , Oliver J. Ratcliffe
IPC分类号： A01H5/00 , A01H1/06 , A01H1/02 , C12N15/82
CPC分类号： C12N15/8202
摘要： Light-regulated promoter sequences were identified that respond to differential light conditions and so can be used to regulate gene expression in a light- or dark-inducible manner. These promoters may be used to produce transgenic plants that have an altered trait relative to control plants. In preferred embodiments, the transgenic plants with the improved traits are morphologically and/or developmentally similar to control plants (examples of the latter include wild-type or non-transformed plants of the same species). Any of these light-regulated promoters may be incorporated into a nucleic acid construct that comprises a polynucleotide regulated by one such promoter and that encodes a polypeptide or RNA molecule that, when ectopically expressed, confers an improved trait in plants.
摘要翻译：鉴定了响应差异光条件的光调节启动子序列，因此可以以浅或暗诱导的方式用于调节基因表达。这些启动子可用于产生相对于对照植物具有改变的性状的转基因植物。在优选的实施方案中，具有改善的性状的转基因植物在形态上和/或发育上与对照植物相似（后者的实例包括相同物种的野生型或非转化植物）。这些光调节启动子中的任何一个可以掺入到包含由一个这样的启动子调控的多核苷酸的核酸构建体中，并且编码多肽或RNA分子，所述多肽或RNA分子当异位表达时赋予植物中改良的性状。

54. 发明申请

US20110179520A1 TISSUE-ENHANCED PROMOTERS 审中-公开
标题翻译：组织增强促进剂
公开(公告)号：US20110179520A1
公开(公告)日：2011-07-21
申请号：US13009039
申请日：2011-01-19
申请人： Suqin CAI , Hans E. Holtan , Peter P. Repetti , T. Lynne Reuber
发明人： Suqin CAI , Hans E. Holtan , Peter P. Repetti , T. Lynne Reuber
IPC分类号： A01H1/00 , C07H21/04 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8226 , C12N15/8223 , C12N15/8225 , C12N15/8229
摘要： Tissue-enhanced promoter sequences were identified that enhance expression of a polypeptide in one or more plant tissues. These promoters may be used to produce transgenic plants that have an altered trait relative to control plants. In preferred embodiments, the transgenic plants with the improved traits are morphologically and/or developmentally similar to control plants (examples of the latter include wild-type or non-transformed plants of the same species). Any of these tissue-enhanced promoters may be incorporated into a nucleic acid construct that comprises a polynucleotide regulated by one such promoter and that encodes a polypeptide or RNA molecule that, when ectopically expressed, confers an improved trait in plants.
摘要翻译：鉴定出增强一个或多个植物组织中多肽表达的组织增强的启动子序列。这些启动子可用于产生相对于对照植物具有改变的性状的转基因植物。在优选的实施方案中，具有改善的性状的转基因植物在形态上和/或发育上与对照植物相似（后者的实例包括相同物种的野生型或非转化植物）。这些组织增强的启动子中的任何一个可以并入包含由一个这样的启动子调节的多核苷酸的核酸构建体，并且编码多肽或RNA分子，当异位表达时赋予植物中改良的性状。

55. 发明申请

US20130219563A1 ENHANCEMENT OF PLANT YIELD VIGOR AND STRESS TOLERANCE 审中-公开
标题翻译：植物营养物质和胁迫耐受性的提高
公开(公告)号：US20130219563A1
公开(公告)日：2013-08-22
申请号：US13780962
申请日：2013-02-28
申请人： Rajnish Khanna , Oliver Ratcliffe , T. Lynne Reuber
发明人： Rajnish Khanna , Oliver Ratcliffe , T. Lynne Reuber
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/113 , C12N15/8218 , C12N15/8262 , C12N15/8269 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： Altering the activity of specific regulatory proteins in plants, for example, by knocking down or knocking out HY5 clade or STH2 clade protein expression, or by modifying COP1 clade protein expression, can have beneficial effects on plant performance, including improved stress tolerance and yield.
摘要翻译：改变植物中特异性调节蛋白的活性，例如通过敲低或敲除HY5进化枝或STH2进化枝蛋白表达，或通过修饰COP1进化枝蛋白表达，可以对植物表现产生有益的影响，包括改善胁迫耐受性和产量。

56. 发明申请

US20110126326A1 STRONG ACTIVATION DOMAIN 审中-公开
标题翻译：强活动域
公开(公告)号：US20110126326A1
公开(公告)日：2011-05-26
申请号：US13000488
申请日：2009-06-26
申请人： Shiv Tiwari , Roger Canales , T. Lynne Reuber , Karen S. Century , Oliver Ratcliffe
发明人： Shiv Tiwari , Roger Canales , T. Lynne Reuber , Karen S. Century , Oliver Ratcliffe
IPC分类号： A01H5/00 , C07K7/06 , C07K7/08 , C07K14/00 , C07H21/00 , C12N5/10 , C12N15/82
摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.
摘要翻译：从拟南芥蛋白质乙烯响应因子98（AtERF98）中鉴定出新的和强的转录激活结构域。该领域已经被指定为“EDLL结构域”，并且具有许多高度保守的氨基酸残基，其在来自植物的AtERF98家族的整个成员中发现，包括单子叶植物和雌蕊直系同源物。当EDLL结构域与植物和酵母的转录因子融合时，显示其具有高度的活性，并且还显示具有与广泛使用的衍生自单纯疱疹的VP16活化区域相当的活化潜力。当通过序列特异性DNA结合蛋白或通过蛋白质 - 蛋白质相互作用靶向基因启动子时，EDLL结构域也是活性的。与其他已知的活化结构域（如VP16和GAL4）不同，EDLL结构域的大小相对较小，并且是植物来源的，因此作为在转基因食品作物中应用的强转录激活工具是有利的。

57. 发明申请

US20110173712A1 ENHANCEMENT OF PLANT YIELD VIGOR AND STRESS TOLERANCE 审中-公开
标题翻译：植物营养物质和胁迫耐受性的提高
公开(公告)号：US20110173712A1
公开(公告)日：2011-07-14
申请号：US12922834
申请日：2009-03-17
申请人： Rajnish Khanna , Oliver Ratcliffe , T. Lynne Reuber
发明人： Rajnish Khanna , Oliver Ratcliffe , T. Lynne Reuber
IPC分类号： A01H1/02 , C07H21/00 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8218 , C12N15/8261 , Y02A40/146
摘要： Altering the activity of specific regulatory proteins in plants, for example, by knocking down or knocking out HY5 clade or STH2 clade protein expression, or by modifying COP1 clade protein expression, can have beneficial effects on plant performance, including improved stress tolerance and yield.
摘要翻译：改变植物中特异性调节蛋白的活性，例如通过敲低或敲除HY5进化枝或STH2进化枝蛋白表达，或通过修饰COP1进化枝蛋白表达，可以对植物表现产生有益的影响，包括改善胁迫耐受性和产量。

58. 发明申请

US20050155117A1 Transcriptional regulation of plant disease tolerance 有权
标题翻译：植物病害耐受性的转录调控
公开(公告)号：US20050155117A1
公开(公告)日：2005-07-14
申请号：US10903236
申请日：2004-07-30
申请人： Karen Century , Emily Queen , T. Lynne Reuber , Oliver Ratcliffe , Roger Canales , Neal Gutterson
发明人： Karen Century , Emily Queen , T. Lynne Reuber , Oliver Ratcliffe , Roger Canales , Neal Gutterson
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8273 , C12N15/8282
摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including disease stress and abiotic stress tolerance, as compared to wild-type or control plants. The invention also pertains to expression systems that may be used to regulate these transcription factor polynucleotides, providing constitutive, transient, inducible and tissue-specific regulation.
摘要翻译：本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同源物，以及与野生型相比，使用多核苷酸和多肽产生具有有利性质的转基因植物（包括疾病胁迫和非生物胁迫耐受性）的方法型或对照植物。本发明还涉及可用于调节这些转录因子多核苷酸的表达系统，提供组成型，瞬时，诱导型和组织特异性调节。

59. 发明申请

US20140123331A1 TRAIT IMPROVEMENT IN PLANTS EXPRESSING AP2 PROTEINS II 审中-公开
标题翻译：表达AP2蛋白的植物中的TRAIT改进II
公开(公告)号：US20140123331A1
公开(公告)日：2014-05-01
申请号：US13799577
申请日：2013-03-13
申请人： Graham J. Hymus , Colleen M. Marion , T. Lynne Reuber , Oliver J. Ratcliffe
发明人： Graham J. Hymus , Colleen M. Marion , T. Lynne Reuber , Oliver J. Ratcliffe
IPC分类号： C12N15/82
CPC分类号： C12N15/8269 , C07K14/415 , C07K14/75 , C12N15/8242 , C12N15/825 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： Polynucleotides and polypeptides incorporated into expression vectors are introduced into plants and were ectopically expressed. These polypeptides may confer at least one regulatory activity and increased photosynthetic resource use efficiency, transpiration efficiency, increased yield, greater vigor, and/or greater biomass as compared to a control plant.
摘要翻译：掺入表达载体的多核苷酸和多肽被引入植物中并被异位表达。与对照植物相比，这些多肽可以赋予至少一种调节活性和增加的光合作用资源利用效率，蒸腾效率，增加的产量，更大的活力和/或更大的生物量。

60. 发明授权

US07960612B2 Plant quality with various promoters 有权
标题翻译：植物质量与各种促进剂
公开(公告)号：US07960612B2
公开(公告)日：2011-06-14
申请号：US12169527
申请日：2008-07-08
申请人： James Z. Zhang , Frederick D. Hempel , Luc J. Adam , Joseph M. Palys , T. Lynne Reuber , Oliver J. Ratcliffe , Robert A. Creelman , Raymond R. Samaha , Pierre E. Broun
发明人： James Z. Zhang , Frederick D. Hempel , Luc J. Adam , Joseph M. Palys , T. Lynne Reuber , Oliver J. Ratcliffe , Robert A. Creelman , Raymond R. Samaha , Pierre E. Broun
IPC分类号： A01H5/00 , A01H5/10 , C12N5/14
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including increased soluble solids, lycopene, and improved plant volume or yield, as compared to wild-type or control plants. The invention also pertains to expression systems that may be used to regulate these transcription factor polynucleotides, providing constitutive, transient, inducible and tissue-specific regulation.
摘要翻译：本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同系物，以及使用多核苷酸和多肽产生具有有利性质的转基因植物的方法，包括增加的可溶性固体，番茄红素和改良的植物体积，产量，与野生型或对照植物相比。本发明还涉及可用于调节这些转录因子多核苷酸的表达系统，提供组成型，瞬时，诱导型和组织特异性调节。

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