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    • 59. 发明授权
    • Screening using polarization anisotropy in FRET emissions
    • 在FRET排放中使用偏振各向异性进行筛选
    • US07674588B2
    • 2010-03-09
    • US12069724
    • 2008-02-11
    • Steven C. MillerPaul B. ComitaChristopher B. ShumateEvan F. Cromwell
    • Steven C. MillerPaul B. ComitaChristopher B. ShumateEvan F. Cromwell
    • C12Q1/68G01N33/53C07H21/02C07H21/04
    • G01N33/542G01N2500/00
    • Methods and apparatus are described for detecting specific binding between first and second chemical entities. The first chemical entity in association with a first fluorophore is immobilized. The second chemical entity is allowed to bind with the immobilized first chemical entity. The second chemical entity is or becomes coupled to a second fluorophore, which forms a FRET pair with the first fluorophore. The bound chemical entities are exposed to radiation at an excitation frequency for either the first or the second fluorophore, and polarization anisotropy of a FRET fluorescent signal from the bound chemical entities is measured to detect specific binding between the first and second chemical entities. Techniques are also disclosed for detecting whether a FRET interaction is occurring between a first chemical entity including a donor fluorophore and a second chemical entity including an acceptor fluorophore, using simultaneous anisotropy measurements at the wavelengths of the donor and acceptor fluorophores.
    • 描述了用于检测第一和第二化学实体之间的特异性结合的方法和装置。 与第一荧光团相关联的第一化学实体被固定化。 允许第二化学实体与固定化的第一化学实体结合。 第二化学实体是或连接到与第一荧光团形成FRET对的第二荧光团。 结合的化学实体以第一或第二荧光团的激发频率暴露于辐射,并且测量来自结合的化学实体的FRET荧光信号的偏振各向异性以检测第一和第二化学实体之间的特异性结合。 还公开了用于检测在包括供体荧光团的第一化学实体和包括受体荧光团的第二化学实体之间是否发生FRET相互作用的技术,其使用在供体和受体荧光团的波长处的同时各向异性测量。
    • 60. 发明授权
    • System and method for providing access to a bus
    • 提供对总线访问的系统和方法
    • US07197589B1
    • 2007-03-27
    • US09315806
    • 1999-05-21
    • Martin M. DeneroffSteven C. Miller
    • Martin M. DeneroffSteven C. Miller
    • G06F13/00G06F13/36
    • G06F13/4068G06F13/364
    • A computer system (10) includes a bus controller (12), a bus (14), a plurality of processing devices (16) and a plurality of enabling switches (18). Each enabling switch (18) corresponds to a separate one of the processing devices (16). Each processing device (16) sends an access request (24) to arbitration logic (22) in the bus controller (12), requesting access to the bus (14). The arbitration logic (22) selects one of the access requests (24) according to a priority protocol. The arbitration logic (22) generates a control signal (20) associated with the selected access request (24). The control signal (20) is provided to the enabling switch (18) corresponding to the processing device (16) that sent the selected access request (24). The enabling switch (18) enables access to the bus (14) for the processing device (16) in response to the control signal (20). In this manner, the computer system (10) can limit a number of processing devices (16) having access to the bus (14) in order to control a load on the bus (14).
    • 计算机系统(10)包括总线控制器(12),总线(14),多个处理设备(16)和多个使能开关(18)。 每个使能开关(18)对应于处理设备(16)中的单独的一个。 每个处理设备(16)向总线控制器(12)中的仲裁逻辑(22)发送访问请求(24),请求访问总线(14)。 仲裁逻辑(22)根据优先级协议选择访问请求(24)之一。 仲裁逻辑(22)产生与所选择的访问请求(24)相关联的控制信号(20)。 控制信号(20)被提供给与发送所选择的访问请求(24)的处理设备(16)相对应的使能开关(18)。 使能开关(18)能够响应于控制信号(20)访问处理设备(16)的总线(14)。 以这种方式,计算机系统(10)可以限制具有访问总线(14)的多个处理设备(16),以便控制总线(14)上的负载。