专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

41. 发明授权

US4657852A Method for B cell typing of total human lymphocyte sample 失效
标题翻译：总人淋巴细胞样本的B细胞分型方法
公开(公告)号：US4657852A
公开(公告)日：1987-04-14
申请号：US616574
申请日：1984-06-04
申请人： Frank C. Grumet , Edgar G. Engleman
发明人： Frank C. Grumet , Edgar G. Engleman
IPC分类号： G01N33/577 , G01N33/80 , G01N33/53 , G01N33/554
CPC分类号： G01N33/80 , G01N33/577 , Y10S435/966 , Y10S435/975 , Y10S436/808 , Y10S436/821
摘要： An HLA-DR typing test based on lymphocytotoxicity in which a vital dye-labeled total human lymphocyte sample, such as a sample of peripheral blood lymphocytes, is incubated with HLA-DR antisera, a monoclonal antibody against T cells, and complement and the DR type is determined based upon the resultant cytotoxicity as measured by the fluorescence of B cells surviving the incubation.
摘要翻译：基于淋巴细胞毒性的HLA-DR分型试验，其中将生物染料标记的总人淋巴细胞样品（例如外周血淋巴细胞样品）与HLA-DR抗血清，针对T细胞的单克隆抗体，以及补体和DR 基于通过孵育中存活的B细胞的荧光测量得到的细胞毒性来确定类型。

42. 发明授权

US4642284A Method and system for detection of complement pathway activation 失效
标题翻译：检测补体通路活化的方法和系统
公开(公告)号：US4642284A
公开(公告)日：1987-02-10
申请号：US503705
申请日：1983-06-13
申请人： Neil Cooper , James T. Mayes
发明人： Neil Cooper , James T. Mayes
IPC分类号： G01N33/53 , C12Q1/00 , G01N33/536 , G01N33/537 , G01N33/564 , G01N33/563
CPC分类号： G01N33/537 , G01N33/564 , Y10S435/965 , Y10S435/966 , Y10S435/971 , Y10S435/975 , Y10S436/808 , Y10S436/821
摘要： A method and system for detecting and preferably measuring the presence of an activated complement complex in a sample is discussed. The presence of such an activated complex is indicative of complement pathway activation and includes a first complement component and a second complement component. The method uses a first binding agent specific to the first complement component and a second binding agent specific to the second complement component which when bound with the complex forms an aggregate. The second specific binding agent includes a label whose presence is used to detect and measure the amount of aggregate and therefore activated complex in a sample. An assay system and aggregate for use in an assay system are also discussed.
摘要翻译：讨论了用于检测并优选测量样品中活化的补体复合物的存在的方法和系统。这种活化的复合物的存在指示补体途径激活，并且包括第一补体成分和第二补体成分。该方法使用特异于第一补体成分的第一结合剂和特异于第二补体成分的第二结合剂，当与复合物结合形成聚集体时。第二种特异性结合剂包括标签，其存在用于检测和测量样品中聚集体的量，并因此测量活化的复合物。还讨论了用于测定系统中的测定系统和聚集体。

43. 发明授权

US4598042A Immunoassay with an increasing cyclic detection system 失效
标题翻译：免疫测定与循环检测系统不断增加
公开(公告)号：US4598042A
公开(公告)日：1986-07-01
申请号：US559489
申请日：1983-12-08
申请人： Colin H. Self
发明人： Colin H. Self
IPC分类号： G01N33/53 , G01N33/58 , C12Q1/32 , C12Q1/42 , G01N33/543
CPC分类号： G01N33/581 , Y10S435/81 , Y10S435/966 , Y10S435/975
摘要： An immunoassay uses an antibody or antigen -enzyme conjugate which converts a precursor to a cycling agent used in a cycling detection system. The amount of cycling agent is continually increased permitting amplification. The prefered system converts NADP to NAD using the conjugate label and the cycling system interconverts NAD and NADH.
摘要翻译：免疫测定使用将前体转化为循环检测系统中使用的循环剂的抗体或抗原 - 酶联结物。循环剂的量不断增加，允许扩增。优选的系统使用共轭标记将NADP转化为NAD，循环系统将NAD和NADH相互转化。

44. 发明授权

US4517289A Monoclonal antibodies for human tissue cross-matching 失效
标题翻译：用于人体组织交叉匹配的单克隆抗体
公开(公告)号：US4517289A
公开(公告)日：1985-05-14
申请号：US409183
申请日：1982-08-18
申请人： Edgar L. Milford , Charles B. Carpenter , Jean M. Paradysz
发明人： Edgar L. Milford , Charles B. Carpenter , Jean M. Paradysz
IPC分类号： G01N33/53 , C07K14/005 , C07K14/195 , C07K16/00 , C07K16/28 , C12N15/02 , C12P21/08 , G01N33/577 , G01N33/54 , A61K39/395 , C12N5/00 , C12N15/00 , C12R1/91
CPC分类号： C07K16/2833 , Y10S435/81 , Y10S435/948 , Y10S435/966 , Y10S435/975 , Y10S436/821 , Y10S530/806 , Y10S530/809 , Y10S530/83
摘要： Immortal, antibody-producing, hybridomally-produced clones which produce antibodies that react specifically with human HLA antigens and which are produced from rat lymphocyte cells sensitized against cells from a rat strain having different histocompatibility antigens are disclosed along with methods of producing the clones and antibodies and methods of using the antibodies in tissue crossing tests.
摘要翻译：公开了产生与人HLA抗原特异性反应的抗体并且由对来自具有不同组织相容性抗原的大鼠菌株的细胞敏感的大鼠淋巴细胞产生的抗体产生的杂交产生的克隆以及产生克隆和抗体的方法以及在组织交叉试验中使用抗体的方法。

45. 发明授权

US4493890A Activated apoglucose oxidase and its use in specific binding assays 失效
标题翻译：活性糖苷氧化酶及其在特异性结合测定中的应用
公开(公告)号：US4493890A
公开(公告)日：1985-01-15
申请号：US404427
申请日：1982-08-02
申请人： David L. Morris
发明人： David L. Morris
IPC分类号： C12Q1/26 , G01N33/542 , G01N33/573 , G01N33/58 , G01N33/54 , C12N9/96
CPC分类号： C12Q1/26 , G01N33/542 , G01N33/573 , G01N33/581 , Y10S435/81 , Y10S435/962 , Y10S435/966 , Y10S435/975
摘要： A method for increasing the ability of apoglucose oxidase to combine with flavin adenine dinucleotide (FAD) and derivatives thereof to form active glucose oxidase by interacting apoglucose oxidase with an immunologically derived binding substance, e.g., an antibody or a fragment thereof, having a specific binding affinity for glucose oxidase. The apoglucose oxidase/anti-glucose oxidase immune complex is characterized by enhanced ability to combine with FAD and FAD-derivatives to yield glucose oxidase activity. The activation is particularly significant at temperatures elevated from room temperature, e.g., between 30.degree.-45.degree. C. An improved homogeneous specific binding assay method is provided for determining ligands wherein an FAD label is used and is monitored by its ability to combine with apoglucose oxidase to form active glucose oxidase by including anti-glucose oxidase in the reaction mixture.
摘要翻译：一种增加糖醛酸氧化酶与黄素腺嘌呤二核苷酸（FAD）及其衍生物结合形成活性葡萄糖氧化酶的能力的方法，其通过与具有特异性结合的免疫学衍生的结合物质（例如抗体或其片段）相互作用而形成葡萄糖氧化酶对葡萄糖氧化酶的亲合力。葡萄糖氧化酶/抗葡萄糖氧化酶免疫复合物的特征在于与FAD和FAD衍生物结合以产生葡萄糖氧化酶活性的能力增强。在从室温升高的温度（例如在30°-45℃之间）的温度下，活化特别显着。提供改进的均相特异性结合测定方法用于测定其中使用FAD标记的配体，并通过其与葡萄糖结合的能力进行监测通过在反应混合物中包含抗葡萄糖氧化酶，氧化酶形成活性葡萄糖氧化酶。

46. 发明授权

US4342826A Immunoassay products and methods 失效
标题翻译：免疫测定产品和方法
公开(公告)号：US4342826A
公开(公告)日：1982-08-03
申请号：US222815
申请日：1981-01-12
申请人： Francis X. Cole
发明人： Francis X. Cole
IPC分类号： A61K9/127 , C07J51/00 , G01N33/542
CPC分类号： C07J51/00 , A61K9/1271 , G01N33/542 , Y10S435/81 , Y10S435/966 , Y10S435/975 , Y10S436/829
摘要： An immunoassay method utilizes antigen tagged, enzyme encapsulating liposomes which are immunospecifically ruptured in the presence of cognate antibody and active complement. A homogeneous phase reaction occurs with the antibody and complement acting to release the enzyme if an immunospecific antigen-antibody complex is formed at the surface of the liposome. The positions of the antigen and antibody can be reversed.
摘要翻译：免疫测定方法利用抗原标记的酶封装脂质体，其在同源抗体和活性补体的存在下被免疫特异性地破裂。如果在脂质体的表面形成免疫特异性抗原 - 抗体复合物，则发生均相反应，抗体和补体作用于释放酶。抗原和抗体的位置可以颠倒。

47. 发明授权

US4220450A Chemically induced fluorescence immunoassay 失效
标题翻译：化学诱导荧光免疫测定
公开(公告)号：US4220450A
公开(公告)日：1980-09-02
申请号：US893910
申请日：1978-04-05
申请人： Edward T. Maggio
发明人： Edward T. Maggio
IPC分类号： G01N33/542 , G01N33/58 , G01N33/16 , C09K11/00 , G01N31/14
CPC分类号： G01N33/581 , G01N33/582 , Y10S435/966 , Y10S435/968 , Y10S436/80 , Y10S436/816 , Y10S436/817
摘要： A competitive protein binding method is provided for the determination of an analyte which is a member of an immunological pair consisting of ligand and receptor for the ligand. A chemiluminescent source is employed comprised of one or more individual members, one chemiluminescent source member being conjugated to one of the members of the immunological pair, so as to provide chemiluminescence adjacent to the site of conjugation. A quencher molecule is conjugated to a member of the immunological pair. When the members of the immunological pair bind, the quencher molecule is brought within quenching distance of the chemiluminescent source so as to inhibit the emission of light by the chemiluminescent source. The amount of analyte present in the assay medium affects the amount of binding between the members of the immunological pair which results in quenching of the chemiluminescence. By observing the light emitted from the assay medium, either from the chemiluminescent source of the quencher, the change in light emission in relation to the concentration of analyte present in the assay medium can be used to determine the amount of analyte present in the assay medium. By employing standards having known amounts of analyte, the amount of analyte in an unknown sample can be quantitatively determined.Reagent kits can be provided having predetermined amounts of the reagents, so as to substantially optimize the sensitivity of the assay.
摘要翻译：提供竞争性蛋白质结合方法用于测定作为配体的配体和受体的免疫对的成员的分析物。使用化学发光源由一个或多个单独的成员组成，一个化学发光源成员与免疫对的一个成员缀合，以提供与缀合位点相邻的化学发光。猝灭剂分子与免疫对的成员缀合。当免疫学对的成员结合时，淬灭剂分子被置于化学发光源的淬灭距离内，以便抑制化学发光源的光的发射。测定培养基中存在的分析物的量影响免疫对的成员之间的结合量，导致化学发光的猝灭。通过从猝灭剂的化学发光源观察从测定介质发射的光，可以使用与测定培养基中存在的分析物浓度相关的光发射变化来确定测定培养基中存在的分析物的量。通过使用具有已知量的分析物的标准品，可以定量测定未知样品中的分析物的量。可以提供具有预定量的试剂的试剂盒，以便基本上优化测定的灵敏度。

48. 发明申请

US20090087864A1 Procedure for the determination of the activity of the protease which activates factor VII from protein solutions 有权
标题翻译：从蛋白质溶液中测定活化因子VII的蛋白酶活性的方法
公开(公告)号：US20090087864A1
公开(公告)日：2009-04-02
申请号：US12155619
申请日：2008-06-06
申请人： Juergen Roemisch , Annette Feussner , Hans-Arnold Stoehr
发明人： Juergen Roemisch , Annette Feussner , Hans-Arnold Stoehr
IPC分类号： G01N33/573 , C12Q1/37
CPC分类号： C12Q1/56 , G01N33/573 , G01N33/86 , G01N2333/96447 , G01N2800/324 , G01N2800/368 , Y10S435/966 , Y10S436/811
摘要： A procedure for the determination of the activity of the protease which activates blood clotting factor VII from protein solutions is describes, in which the protein solution comprising the protease and/or its proenzyme is incubated with a solid phase to which an antibody which binds the protease and/or its proenzyme has been coupled beforehand, and after washing the solid phase, the protease and/or its proenzyme fixed thereto, are incubated with reagents which allow for the determination of their activity.
摘要翻译：描述了用于测定蛋白酶活化凝血因子VII的蛋白酶的活性的方法，其中将包含蛋白酶和/或其酶原的蛋白质溶液与固相结合，所述固相与结合蛋白酶的抗体和/或其酶原已经预先偶联，并且在洗涤固相之后，将固定在其上的蛋白酶和/或其酶原与用于测定其活性的试剂一起温育。

49. 发明授权

US06783731B1 Dry analytical element for acetaminophen assay 失效
标题翻译：对乙酰氨基酚测定用干燥分析元素
公开(公告)号：US06783731B1
公开(公告)日：2004-08-31
申请号：US08493442
申请日：1995-06-22
申请人： Thomas C. Arter , John C. Mauck , James R. Schaeffer , Robert F. Winterkorn
发明人： Thomas C. Arter , John C. Mauck , James R. Schaeffer , Robert F. Winterkorn
IPC分类号： G01N2100
CPC分类号： C12Q1/34 , G01N2333/98 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/815
摘要： A spectrophotometric assay for the detection of acetaminophen in aqueous fluids is carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; and a mild oxidizing agent to oxidize the p-aminophenol so that it couples to a water-soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.
摘要翻译：用水分液体检测对乙酰氨基酚的分光光度法用干燥分析元件进行。元件包括其上具有一个或多个试剂层的载体，其含有第一种酶，芳基酰胺酶，以切割对乙酰氨基酚的酰胺键以产生对氨基苯酚; 和一种温和的氧化剂，以氧化对氨基苯酚，使其与水溶性偶联剂偶联形成在670nm读取的染料。该测定法在血清和血浆样品中精确，准确，并且相对没有显着的干扰。该元件还允许在宽动态范围内进行测量。

50. 发明申请

US20040023272A1 Method for detecting biological agents 失效
标题翻译：检测生物制剂的方法
公开(公告)号：US20040023272A1
公开(公告)日：2004-02-05
申请号：US10412568
申请日：2003-04-10
发明人： Liaohai Chen , Duncan W. McBranch , Hsing-Lin Wang , David G. Whitten
IPC分类号： C12Q001/68 , C12M001/34 , C12M003/00 , G01N033/543
CPC分类号： G01N33/54373 , C07B2200/05 , C07C319/14 , G01N33/5438 , G01N33/54386 , Y10S435/808 , Y10S435/966 , Y10S436/805 , Y10S436/806 , Y10S436/808 , Y10S436/823 , Y10S530/811 , Y10S530/812 , C07C323/12
摘要： A sensor is provided including a polymer capable of having an alterable measurable property from the group of luminescence and electrical conductivity, the polymer having an intermediate combination of a recognition element, a tethering element and a property-altering element bound thereto and capable of altering the measurable property, the intermediate combination adapted for subsequent separation from the polymer upon exposure to an agent having an affinity for binding to the recognition element whereupon the separation of the intermediate combination from the polymer results in a detectable change in the alterable measurable property, and, a means of detecting said detectable change in the alterable measurable property.
摘要翻译：提供了一种传感器，其包括能够从发光和导电性组中具有可改变的可测量性质的聚合物，所述聚合物具有识别元件，束缚元件和结合到其上的改变属性的元件的中间组合，并且能够改变可测量的性质，适于在暴露于具有与识别元件结合的亲和力的试剂之后随后与聚合物分离的中间体组合，因此中间体组合与聚合物的分离导致可改变的可测量性质的可检测变化，检测可变化可测量性质中所述可检测变化的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式