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41. 发明申请

US20150322408A1 Micro Blood Vessels and Tissue Ducts 审中-公开
公开(公告)号：US20150322408A1
公开(公告)日：2015-11-12
申请号：US14789269
申请日：2015-07-01
申请人： André A. Adams , Michael Daniele , Frances S. Ligler
发明人： André A. Adams , Michael Daniele , Frances S. Ligler
IPC分类号： C12N5/071
CPC分类号： C12N5/0697 , A61F2240/001 , B01J19/0093 , B01J2219/00783 , B01J2219/00831 , B01J2219/00833 , B01J2219/0086 , B01J2219/00873 , B01J2219/00889 , B01J2219/00891 , B01J2219/0093 , B01J2219/00936 , B01L3/502707 , B01L3/502746 , B01L3/502776 , B01L2200/0636 , B01L2200/10 , B01L2200/12 , B01L2300/0838 , B01L2300/0887 , B01L2300/12 , B01L2300/16 , B01L2400/0487 , B01L2400/084 , B29D99/0078 , B29K2995/0056 , B29K2995/006 , B29L2009/00 , B29L2023/00 , C12M21/08 , C12M25/10 , C12M25/12 , C12M25/14 , C12N5/0602 , G02B6/032 , G02B2006/0325
摘要： A fiber includes one or more layers of polymer surrounding a central lumen, and living animal cells disposed within the lumen and/or within at least one of the one or more layers, wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns. Also disclosed are model tissues including such fibers, and method of making such fibers. The fibers can serve as synthetic blood vessels, ducts, or nerves.

42. 发明授权

US09057045B2 Method of loading and distributing cells in a bioreactor of a cell expansion system 有权
标题翻译：在细胞扩增系统的生物反应器中负载和分配细胞的方法
公开(公告)号：US09057045B2
公开(公告)日：2015-06-16
申请号：US12968483
申请日：2010-12-15
申请人： David M. Gibbons , Brian J. Nankervis
发明人： David M. Gibbons , Brian J. Nankervis
IPC分类号： C12N5/071 , C12M1/00 , C12M3/00 , C12M1/12 , C12M3/06
CPC分类号： C12M29/16 , C12M23/42 , C12M23/50 , C12M25/10 , C12M25/12 , C12M27/14
摘要： A method of distributing a plurality of cells in a bioreactor of a cell expansion system includes manipulating an orientation of the bioreactor such that a net impulse due to gravity acting on the plurality cells in the bioreactor is reduced. One method includes (a) rotating the bioreactor at an angular velocity ω about an axis of rotation and through an angular displacement θ, the bioreactor rotating from a first orientation to a second orientation; (b) holding the bioreactor still at the second orientation for a first period of time t1, wherein t1 substantially equals 2/ω; (c) rotating the bioreactor at the angular velocity ω about the axis of rotation and through the angular displacement θ; and (d) holding the bioreactor still for a second period of time t2, wherein t2 substantially equals t1.
摘要翻译：在细胞扩增系统的生物反应器中分配多个细胞的方法包括操纵生物反应器的取向，使得由于重力作用在生物反应器中的多个细胞上的净冲击减少。一种方法包括：（a）以围绕旋转轴线的角速度ω旋转生物反应器并通过角度位移旋转生物反应器;生物反应器从第一取向旋转到第二取向; （b）将所述生物反应器保持在所述第二方向上第一时间段t1，其中t1基本上等于2 /ω; （c）以关于旋转轴线和角位移的角速度ω旋转生物反应器; 和（d）将生物反应器保持第二时间段t2，其中t2基本上等于t1。

43. 发明申请

US20150140653A1 EXPANDING CELLS IN A BIOREACTOR 有权
标题翻译：在生物反应器中扩增细胞
公开(公告)号：US20150140653A1
公开(公告)日：2015-05-21
申请号：US14542276
申请日：2014-11-14
申请人： Terumo BCT, Inc.
发明人： Mark E. JONES , Nathan D. FRANK
IPC分类号： C12M1/00 , C12M1/12 , C12M3/04 , C12N5/0775 , C12M1/36
CPC分类号： C12M29/18 , C12M23/50 , C12M25/10 , C12M25/12 , C12M27/10 , C12M29/10 , C12M29/16 , C12M41/48 , C12N5/0663
摘要： Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.
摘要翻译：描述了用于在生物反应器中扩展细胞的实施方案。在一个实施方案中，提供了将细胞分布在整个生物反应器中并将细胞附着到生物反应器的特定部分以改善细胞在生物反应器中的扩增的方法。实施例可以在被配置为加载，分发，附加和扩展单元的单元扩展系统上实现。

44. 发明申请

US20150011004A1 Systems and Methods for Expanding High Density Non-Adherent Cells 审中-公开
标题翻译：用于扩增高密度非粘附细胞的系统和方法
公开(公告)号：US20150011004A1
公开(公告)日：2015-01-08
申请号：US14492930
申请日：2014-09-22
申请人： Terumo BCT, Inc.
发明人： Glen Delbert ANTWILER
IPC分类号： C12M1/00
CPC分类号： C12M29/18 , C12M23/02 , C12M23/58 , C12M25/12 , C12M29/16
摘要： Embodiments described herein generally relate to systems and methods for promoting the expansion of high density non-adherent cells through the use of a cell growth chamber, a mass transfer device, and a fluid circulation loop. Improved cell growth is achieved in the cell growth chamber by using a chamber having a particular orientation and shape, e.g., conical, to create a media-rich reservoir for growing cells. By placing the chamber in a vertical position, the force of media flow along the chamber walls is substantially equal and opposite to the gravitational force on the cells. The interaction of these forces maintains the non-adherent cells in suspension. The use of the cell growth chamber in conjunction with the mass transfer device and fluid circulation loop(s) creates efficiencies by relying on the cumulative and combined features of the devices.
摘要翻译：本文描述的实施方案通常涉及通过使用细胞生长室，质量传递装置和流体循环回路促进高密度非粘附细胞的扩增的系统和方法。通过使用具有特定取向和形状（例如圆锥形）的室，在细胞生长室中实现改善的细胞生长，以产生用于生长细胞的富含培养基的储存器。通过将腔室放置在垂直位置，介质沿腔室壁流动的力基本相等并与细胞上的重力相反。这些力的相互作用使非粘附细胞保持悬浮状态。与传质装置和流体循环回路结合使用细胞生长室通过依赖于装置的累积和组合特征来产生效率。

45. 发明授权

US08906688B2 Cell expansion system and methods of use 有权
标题翻译：细胞扩增系统及使用方法
公开(公告)号：US08906688B2
公开(公告)日：2014-12-09
申请号：US12102413
申请日：2008-04-14
申请人： Glen Delbert Antwiler
发明人： Glen Delbert Antwiler
IPC分类号： C12N5/02 , C12N5/00 , C12M1/34 , C12M1/12 , C12M1/00
CPC分类号： C12M41/00 , C12M25/12 , C12M29/16 , C12N5/0068
摘要： Cell expansion systems are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second fluid flow paths associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. The hollow fibers have a hydrophilic interior surface and a hydrophobic exterior surface. Detachable flow circuits are also provided.
摘要翻译：提供电池膨胀系统。细胞膨胀系统通常包括中空纤维细胞生长室，以及分别与中空纤维的内部和中空纤维的外部相关联的第一和第二流体流动路径。中空纤维具有亲水性内表面和疏水性外表面。还提供了可拆卸的流动回路。

46. 发明授权

US08809043B2 Rotation system for cell growth chamber of a cell expansion system and method of use therefor 有权
标题翻译：细胞扩张系统细胞生长室旋转系统及其使用方法
公开(公告)号：US08809043B2
公开(公告)日：2014-08-19
申请号：US13765857
申请日：2013-02-13
申请人： Terumo BCT, Inc.
发明人： Paul M. Leuthaeuser , Jon A. Dodd , Thomas G. Dilorenzo
IPC分类号： C12M1/10 , B01F11/00 , C12M1/00 , C12M3/00 , C12M3/04 , C12M3/06
CPC分类号： C12M23/50 , B01F11/00 , B01F11/0005 , C12M23/42 , C12M23/48 , C12M25/10 , C12M25/12 , C12M27/10 , C12M27/14 , C12M27/16 , C12N5/0068
摘要： A system and method for rotating a cell growth chamber of a cell expansion system includes a rotatable member for engaging a chamber coupling attached to the cell growth chamber. The rotatable member includes an independently operable mechanism for engaging a rotatable fitting associated with the chamber coupling. In at least one embodiment, the chamber coupling is selectively rotatable by turning the rotatable member, thereby rotating the cell growth chamber around a first axis. The cell growth chamber is also selectively rotatable around a second axis by turning the rotatable fitting associated with the chamber coupling. Other novel aspects include a way of attaching the cell growth chamber to the shaft assembly, and a new tube routing clip.
摘要翻译：用于旋转电池膨胀系统的电池生长室的系统和方法包括用于接合附接到电池生长室的室耦合的可旋转构件。可旋转构件包括用于接合与腔室联接器相关联的可旋转配件的可独立操作的机构。在至少一个实施例中，通过转动可旋转构件来选择性地旋转室联接器，从而使细胞生长室围绕第一轴线旋转。通过转动与腔室联接件相关联的可旋转配件，细胞生长室也可以围绕第二轴线选择性地旋转。其他新颖的方面包括将细胞生长室附接到轴组件的方式和新的管路径夹。

47. 发明申请

US20140178995A1 Method of Reseeding Adherent Cells Grown in a Hollow Fiber Bioreactor System 有权
标题翻译：在中空纤维生物反应器系统中生长的粘附细胞的补充方法
公开(公告)号：US20140178995A1
公开(公告)日：2014-06-26
申请号：US14192370
申请日：2014-02-27
申请人： Terumo BCT, Inc.
发明人： Glen Delbert ANTWILER , David A. WINDMILLER , Monique GIVENS
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12M25/10 , C12M25/12 , C12N5/0025 , C12N2500/14 , C12N2500/16 , C12N2500/34 , C12N2509/00
摘要： This invention is directed to methods of directly reseeding harvested adherent cells grown in a hollow fiber bioreactor. Also disclosed is a novel harvest media for use in directly reseeding adherent cells into a hollow fiber bioreactor.
摘要翻译：本发明涉及在中空纤维生物反应器中生长的收获的贴壁细胞的直接再混合的方法。还公开了一种新颖的收获介质，用于将贴壁细胞直接再切割成中空纤维生物反应器。

48. 发明申请

US20120088224A1 Methods and Systems of Growing and Harvesting Cells in a Hollow Fiber Bioreactor System with Control Conditions 有权
标题翻译：在具有控制条件的中空纤维生物反应器系统中生长和收获细胞的方法和系统
公开(公告)号：US20120088224A1
公开(公告)日：2012-04-12
申请号：US13269512
申请日：2011-10-07
申请人： Thomas G. DILORENZO , Edward Allan STANTON, IV , Glen Delbert ANTWILER , Michael E. KINZIE , Brian J. Nankervis , Monique Givens , Casey V. Medina , Jon A. Dodd
发明人： Thomas G. DILORENZO , Edward Allan STANTON, IV , Glen Delbert ANTWILER , Michael E. KINZIE , Brian J. Nankervis , Monique Givens , Casey V. Medina , Jon A. Dodd
IPC分类号： C12Q3/00 , C12M1/36
CPC分类号： C12M41/44 , C12M23/42 , C12M23/44 , C12M25/10 , C12M25/12 , C12M29/16 , C12M29/20 , C12M41/48 , G06F8/38
摘要： Embodiments described herein generally relate to methods and systems for using an air removal chamber as a control for a process in a cell expansion system. The air removal chamber may be mounted on a fluid conveyance assembly for use with the system. Fluid is pumped into a fluid containment chamber of the air removal chamber, in which the level of fluid in the fluid containment chamber may be monitored through the use of one or more sensors. The sensors are capable of detecting air, a lack of fluid, fluid, and/or a gas/fluid interface, e.g., an air/fluid interface, at measuring positions within the air removal chamber. Protocols for use with the system may include one or more stop conditions. In an embodiment, the stopping of a process is automated based on the detection of air, a lack of fluid, and/or a gas/fluid interface in the air removal chamber.
摘要翻译：本文描述的实施例一般涉及使用空气除去室作为电池膨胀系统中的工艺的控制的方法和系统。排气室可以安装在流体输送组件上以与系统一起使用。流体被泵送到空气移除室的流体容纳室中，其中流体容纳室中的流体水平可以通过使用一个或多个传感器来监测。传感器能够在空气去除室内的测量位置处检测空气，缺乏流体，流体和/或气体/流体界面，例如空气/流体界面。用于该系统的协议可以包括一个或多个停止条件。在一个实施例中，基于在空气移除室中检测空气，缺乏流体和/或气体/流体界面，自动停止过程。

49. 发明申请

US20120086657A1 Configurable Methods and Systems of Growing and Harvesting Cells in a Hollow Fiber Bioreactor System 有权
标题翻译：中空纤维生物反应器系统中生长和收获细胞的可配置方法和系统
公开(公告)号：US20120086657A1
公开(公告)日：2012-04-12
申请号：US13269323
申请日：2011-10-07
申请人： Edward Allan STANTON, IV , Glen Delbert ANTWILER , Patrick J. HOWLEY , Michael E. KINZIE , Jon A. DODD , Casey V. MEDINA
发明人： Edward Allan STANTON, IV , Glen Delbert ANTWILER , Patrick J. HOWLEY , Michael E. KINZIE , Jon A. DODD , Casey V. MEDINA
IPC分类号： G06F3/048 , G06F3/041 , C12M1/00
CPC分类号： C12M41/44 , C12M23/42 , C12M23/44 , C12M25/10 , C12M25/12 , C12M29/16 , C12M29/20 , C12M41/48 , G06F8/38
摘要： Embodiments described herein generally relate to methods and systems for configuring settings of a cell expansion system including a bioreactor. Through a user interface, a user may configure display settings, system settings, and settings associated with protocols for loading, growing and/or harvesting cells. In configuring settings for protocols and associated processes, a diagram view or window of the cell expansion system is displayed in embodiments. The diagram view displays the process settings as graphical user interface elements. Settings available for configuration are enabled for selection in the diagram view. The diagram view allows the user to visualize the settings available for task configuration and to configure enabled settings. Configured settings are stored and capable of retrieval for subsequent execution or modification of the applicable protocol.
摘要翻译：本文描述的实施例通常涉及用于配置包括生物反应器的细胞扩增系统的设置的方法和系统。通过用户界面，用户可以配置显示设置，系统设置和与用于加载，增长和/或收集单元的协议相关联的设置。在配置协议和关联进程的设置时，在实施例中显示单元扩展系统的图表视图或窗口。图表视图将进程设置显示为图形用户界面元素。可用于配置的设置在图表视图中启用选择。图表视图允许用户可视化可用于任务配置的设置，并配置启用的设置。存储配置的设置并能够检索以便后续执行或修改适用的协议。

50. 发明授权

US5981211A Maintaining cells for an extended time by entrapment in a contracted matrix 失效
标题翻译：通过在收缩的矩阵中捕获来维持细胞长时间
公开(公告)号：US5981211A
公开(公告)日：1999-11-09
申请号：US726652
申请日：1996-10-07
申请人： Wei-Shou Hu , Frank Bernard Cerra , Scott Lyle Nyberg , Matthew Thomas Scholz , Russell A. Shatford
发明人： Wei-Shou Hu , Frank Bernard Cerra , Scott Lyle Nyberg , Matthew Thomas Scholz , Russell A. Shatford
IPC分类号： A61F2/02 , A61K35/12 , A61M1/16 , A61M1/34 , C12M3/06 , C12N5/071 , C12P1/00 , C12M3/00 , C12N5/00 , C12N11/04
CPC分类号： C12N5/0671 , A61F2/022 , A61M1/1625 , A61M1/3475 , A61M1/3489 , C12M21/08 , C12M23/34 , C12M25/12 , C12M25/14 , C12M29/10 , C12M29/16 , A61K2035/126 , C12N2500/02 , C12N2500/90 , C12N2510/00 , C12N2533/30 , C12N2533/54 , Y10S435/813
摘要： Methods of maintaining animal cells for product production, for supporting hepatocyte function and viability to treat a patient suffering from hepatic failure and for preserving tissue-specific function of mammalian cells are carried out with a bioreactor containing a feed and waste chamber and a cell chamber separated by a selectively permeable membrane. Within the cell chamber, a biocompatible contracted three-dimensional gel matrix entraps animal cells or genetic modifications thereof, and a liquid phase contains a concentrated solution of the cell product. The bioreactor uses only two chambers to achieve three distinct zones within the bioreactor. The bioreactor can be of either hollow fiber or flat-bed configuration. In the configuration using hollow fibers, the two fluid paths correspond to the cavity surrounding the hollow fibers (the extracapillary space), and to the lumens of the hollow fibers themselves. Both fluid paths have inlet and outlet ports. Communication between the two fluid paths is across the permeable medium--the hollow fiber material. To prepare a bioartificial liver, hepatocytes are inoculated into the hollow fibers in a solution which quickly forms a highly porous gel. The gel subsequently contracts, leaving an open channel within the hollow fiber adjacent to the gel core entrapped hepatocytes. This channel can be perfused with nutrient media for hepatocytes. The channel can also serve as a waste stream to remove toxins that the hepatocytes have modified to a water soluble form.
摘要翻译：维持用于产品生产的动物细胞，用于支持肝细胞功能和用于治疗患有肝衰竭的患者和维持哺乳动物细胞的组织特异性功能的活性的方法是用含有饲料和废物室的细胞室和分离的细胞室进行的通过选择性渗透膜。在细胞室内，生物相容的收缩的三维凝胶基质包裹动物细胞或其遗传修饰，液相含有细胞产物的浓缩溶液。生物反应器仅使用两个室来实现生物反应器内的三个不同区域。生物反应器可以是中空纤维或平床构型。在使用中空纤维的构造中，两个流体路径对应于围绕中空纤维（毛细管外空间）的空腔以及中空纤维本身的内腔。两个流体路径都具有入口和出口。两个流体路径之间的通信是穿过可渗透介质 - 中空纤维材料。为了制备生物人造肝，将肝细胞以快速形成高度多孔凝胶的溶液接种到中空纤维中。凝胶随后收缩，在中空纤维中留下与凝胶芯捕获的肝细胞相邻的开放通道。该通道可以用肝细胞的营养培养基灌注。该通道还可用作废物流以除去肝细胞已经修饰成水溶性形式的毒素。

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