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41. 发明授权

US07470787B2 Isoquinoline compounds 有权
标题翻译：异喹啉化合物
公开(公告)号：US07470787B2
公开(公告)日：2008-12-30
申请号：US11485172
申请日：2006-07-11
申请人： Mitchell A. deLong , Marcos L. Sznaidman , Robert H. Oakley , Allen E. Eckhardt , Christine Hudson , Jeffrey D. Yingling , Michael Peel , Thomas E. Richardson , Claire Louise Murray , Byappanahally N. Narasinga Rao , Brian H. Heasley , Paresma R. Patel
发明人： Mitchell A. deLong , Marcos L. Sznaidman , Robert H. Oakley , Allen E. Eckhardt , Christine Hudson , Jeffrey D. Yingling , Michael Peel , Thomas E. Richardson , Claire Louise Murray , Byappanahally N. Narasinga Rao , Brian H. Heasley , Paresma R. Patel
IPC分类号： C07D217/00 , C07D401/00 , C07D215/00
CPC分类号： C07D217/02
摘要： Isoquinoline compounds with G are provided that influence, inhibit or reduce the action of a G-protein receptor kinase. Pharmaceutical compositions including therapeutically effective amounts of the isoquinoline compounds and pharmaceutically acceptable carriers are also provided. Various methods using the compounds and/or compositions to affect disease states or conditions such as cancer, osteoporosis and glaucoma are also provided.
摘要翻译：提供了具有G的异喹啉化合物，其影响，抑制或降低G蛋白受体激酶的作用。还提供包含治疗有效量的异喹啉化合物和药学上可接受的载体的药物组合物。还提供了使用化合物和/或组合物影响疾病状态或病症如癌症，骨质疏松症和青光眼的各种方法。

42. 发明授权

US07297503B2 Methods of identifying reduced internalization transmembrane receptor agonists 有权
标题翻译：鉴定减少的内化跨膜受体激动剂的方法
公开(公告)号：US07297503B2
公开(公告)日：2007-11-20
申请号：US10693164
申请日：2003-10-24
申请人： Carson R Loomis , Robert H. Oakley , Shuntai Wang , Allen E. Eckhardt
发明人： Carson R Loomis , Robert H. Oakley , Shuntai Wang , Allen E. Eckhardt
IPC分类号： G01N33/53 , G01N33/567 , G01N33/542 , C12Q1/66
CPC分类号： G01N33/566 , G01N2500/04
摘要： Described herein are methods of identifying a transmembrane receptor (TMR) agonist and compounds identified by this method. The TMR agonist (TMRA) is capable of activating TMR signaling while exhibiting reduced TMR internalization over a control compound.
摘要翻译：本文描述了鉴定跨膜受体（TMR）激动剂和通过该方法鉴定的化合物的方法。 TMR激动剂（TMRA）能够激活TMR信号，同时在对照化合物上显示降低的TMR内化。

43. 发明授权

US06346387B1 Detection of binding reactions using labels detected by mediated catalytic electrochemistry 有权
标题翻译：使用通过介导的催化电化学检测的标记检测结合反应
公开(公告)号：US06346387B1
公开(公告)日：2002-02-12
申请号：US09722065
申请日：2000-11-24
申请人： David H. Stewart , John W. Groelke , H. Holden Thorp , Allen E. Eckhardt
发明人： David H. Stewart , John W. Groelke , H. Holden Thorp , Allen E. Eckhardt
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B82Y5/00 , C12Q1/6825 , G01N33/5438 , C12Q2565/607 , C12Q2563/137 , C12Q2563/113
摘要： A method of detecting binding interactions and target molecules, such as proteins, protein fragments, recombinant proteins, recombinant protein fragments, extracellular matrix proteins, ligands, carbohydrates, steroids, hormones, drugs, drug candidates, immunoglobulins and receptors of eukaryotic, prokaryotic or viral origin, by mediated electrochemistry using labels that react with transition metal mediator complexes in a detectable catalytic redox reaction. These labels are attached directly to binders, target molecules, surrogate target molecules, or to affinity ligands capable of binding to the target or to surrogate target molecules capable of competing with the target for binding to another binder. The labels can be naturally present (endogenous) in the binder, target or affinity ligand, or constructed by the covalent attachment of the label to the binder, target, affinity ligand or surrogate target (exogenous).
摘要翻译：检测结合相互作用和靶分子的方法，例如蛋白质，蛋白质片段，重组蛋白，重组蛋白片段，细胞外基质蛋白，配体，碳水化合物，类固醇，激素，药物，候选药物，免疫球蛋白和真核，原核或病毒的受体通过介导的电化学使用在可检测的催化氧化还原反应中与过渡金属介体络合物反应的标记。这些标记物直接连接到粘合剂，靶分子，替代靶分子，或与能够结合靶的亲和配体或替代能与靶标竞争结合另一种粘合剂的靶分子。标签可以在粘合剂，靶或亲和配体中天然存在（内源的），或者通过标记共价连接到粘合剂，靶，亲和配体或替代靶（外源的）上构建。

44. 发明申请

US20100151439A1 Enzymatic Assays for a Droplet Actuator 有权
标题翻译：滴液致动器的酶检测
公开(公告)号：US20100151439A1
公开(公告)日：2010-06-17
申请号：US12531844
申请日：2008-03-23
申请人： Vamsee K. Pamula , Allen E. Eckhardt
发明人： Vamsee K. Pamula , Allen E. Eckhardt
IPC分类号： C12Q1/00 , C12N9/00
CPC分类号： G01N21/6486 , B01F13/0809 , C07H15/04 , C12Q1/34 , G01N2333/928 , G01N2800/042
摘要： A method of conducting a droplet-based enzymatic assay is provided. The method generally makes use of a droplet actuator. A droplet comprising an enzyme of interest is provided on the droplet actuator along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. The method involves executing droplet operations on the droplet actuator to combine the droplets, thereby yielding an assay droplet, and detecting modification of the substrate by the enzyme in the assay droplet on the droplet actuator. The enzyme of interest may, for example, be a potentially mutated or improperly folded enzyme exhibiting altered enzyme activity as compared to a corresponding normal enzyme.
摘要翻译：提供了一种进行基于液滴的酶测定的方法。该方法通常使用液滴致动器。包含感兴趣的酶的液滴与包含底物的液滴一起提供在液滴致动器上，所述液滴在酶的存在下可能被修饰。该方法涉及对液滴致动器执行液滴操作以组合液滴，从而产生测定液滴，并且通过液滴致动器上的测定液滴中的酶检测底物的修饰。感兴趣的酶可以是例如与相应的正常酶相比显示改变的酶活性的潜在突变或不正确折叠的酶。

45. 发明授权

US06387625B1 Monolayer and electrode for detecting a label-bearing target and method of use thereof 有权
标题翻译：用于检测标记物的单层和电极及其使用方法
公开(公告)号：US06387625B1
公开(公告)日：2002-05-14
申请号：US09596607
申请日：2000-06-19
申请人： Allen E. Eckhardt , Jill C. Mikulecky , Mary E. Napier , Robert S. Thomas , H. Holden Thorp
发明人： Allen E. Eckhardt , Jill C. Mikulecky , Mary E. Napier , Robert S. Thomas , H. Holden Thorp
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B82Y15/00 , C12Q1/6816 , C12Q1/6825 , Y10S435/81 , Y10T436/143333 , C12Q2525/197 , C12Q2563/113
摘要： An electrode for detecting interactions between members of a binding pair, which electrode has been modified by formation of a non-conductive self-assembled monolayer, and a method of detecting biomolecules, such as nucleic acids or other targets, including receptors, ligands, antigens or antibodies, utilizing such an electrode. When contacted with a target nucleic acid, an oligonucleotide probe coupled to the self-assembled monolayer reacts with the target nucleic acid form a hybridized nucleic acid on the modified electrode surface. The hybridized nucleic acid is reacted with a transition metal complex capable of oxidizing a preselected base in the hybridized nucleic acid in an oxidation-reduction reaction, the oxidation-reduction reaction is detected, and the presence or absence of the nucleic acid is determined from the detected oxidation-reduction reaction.
摘要翻译：用于检测结合对的成员之间的相互作用的电极，该电极已经通过形成非导电自组装单层而被修饰，以及检测生物分子例如核酸或其它靶，包括受体，配体，抗原的方法或抗体，利用这种电极。当与目标核酸接触时，与自组装单层结合的寡核苷酸探针与目标核酸反应，形成经修饰的电极表面的杂交核酸。杂交的核酸与氧化还原反应中能够氧化杂交核酸中的预选碱基的过渡金属络合物反应，检测氧化还原反应，核酸的存在或不存在从检测到氧化还原反应。

46. 发明授权

US08901043B2 Systems for and methods of hybrid pyrosequencing 有权
标题翻译：混合焦磷酸测序系统和方法
公开(公告)号：US08901043B2
公开(公告)日：2014-12-02
申请号：US13542721
申请日：2012-07-06
申请人： Allen E. Eckhardt , Jonathan Benton , Deborah Boles
发明人： Allen E. Eckhardt , Jonathan Benton , Deborah Boles
IPC分类号： C40B30/00 , C12N15/10 , C12Q1/68 , G06F19/22 , G06F19/24
CPC分类号： C12N15/1089 , C12Q1/6869 , C12Q1/689 , C12Q1/701 , C12Q1/703 , G06F19/22 , G06F19/24 , C12Q2537/165 , C12Q2565/301
摘要： The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.
摘要翻译：本发明的系统和方法提供了焦磷酸测序（即，杂化焦磷酸测序）的指导方法。从头核酸序列可以与可能结果的文库进行比较，并且基于从头序列与可能结果库的比较来选择待分配的下一个核苷酸。在另一个实例中，基于对非序列参数（例如，感染发生率，诊断症状，样本来源）的数据库的查询和基于比较确定的随后分配来选择至少要分配的第一核苷酸的新生序列和可能结果的库（例如候选序列）。本发明的系统和方法可以使用液滴致动器来执行。

47. 发明申请

US20100028920A1 Hydrogen Peroxide Droplet-Based Assays 有权
标题翻译：过氧化氢液滴检测
公开(公告)号：US20100028920A1
公开(公告)日：2010-02-04
申请号：US12529052
申请日：2008-03-05
申请人： Allen E. Eckhardt
发明人： Allen E. Eckhardt
IPC分类号： C12Q1/54 , C12Q1/28
CPC分类号： C12Q1/28 , C12Q1/005 , C12Q1/006 , G01N2333/904 , Y10T436/118339 , Y10T436/14 , Y10T436/141111 , Y10T436/142222 , Y10T436/143333 , Y10T436/144444 , Y10T436/145555 , Y10T436/146666 , Y10T436/147777 , Y10T436/148888 , Y10T436/206664
摘要： The present invention relates to providing improved hydrogen peroxide assays, as well as droplet actuators for conducting such assays. The droplet actuators of the invention may be used to conduct droplet-based hydrogen peroxide assays. They may also be associated with detectors for analyzing the results of the hydrogen peroxide assays of the invention. They may be provided as components of systems which control droplet operations and/or detection for conducting the hydrogen peroxide assays. Measurement by the detector may be used to quantify the presence of an analyte in a sample.
摘要翻译：本发明涉及提供改进的过氧化氢测定，以及用于进行这种测定的液滴致动器。本发明的液滴致动器可用于进行基于液滴的过氧化氢测定。它们也可以与用于分析本发明的过氧化氢测定结果的检测器相关联。它们可以作为控制液滴操作和/或检测以进行过氧化氢测定的系统的组件提供。检测器的测量可用于量化样品中分析物的存在。

48. 发明授权

US6127127A Monolayer and electrode for detecting a label-bearing target and method of use thereof 有权
标题翻译：用于检测标记物的单层和电极及其使用方法
公开(公告)号：US6127127A
公开(公告)日：2000-10-03
申请号：US296929
申请日：1999-04-22
申请人： Allen E. Eckhardt , Jill C. Mikulecky , Mary E. Napier , Robert S. Thomas , H. Holden Thorp
发明人： Allen E. Eckhardt , Jill C. Mikulecky , Mary E. Napier , Robert S. Thomas , H. Holden Thorp
IPC分类号： G01N33/483 , C07F9/22 , C07K1/113 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , G01N33/53 , G01N33/543 , G01N33/547 , G01N33/553 , G01N33/566
CPC分类号： B82Y30/00 , B82Y15/00 , C12Q1/6825
摘要： An electrode for detecting interactions between members of a binding pair, which electrode has been modified by formation of a non-conductive self-assembled monolayer, and a method of detecting biomolecules, such as nucleic acids or other targets, including receptors, ligands, antigens or antibodies, utilizing such an electrode. When contacted with a target nucleic acid, an oligonucleotide probe coupled to the self-assembled monolayer reacts with the target nucleic acid to form a hybridized nucleic acid on the modified electrode surface. The hybridized nucleic acid is reacted with a transition metal complex capable of oxidizing a preselected base in the hybridized nucleic acid in an oxidation-reduction reaction, the oxidation-reduction reaction is detected, and the presence or absence of the nucleic acid is determined from the detected oxidation-reduction reaction.
摘要翻译：用于检测结合对的成员之间的相互作用的电极，该电极已经通过形成非导电自组装单层而被修饰，以及检测生物分子例如核酸或其它靶，包括受体，配体，抗原的方法或抗体，利用这种电极。当与靶核酸接触时，与自组装单层结合的寡核苷酸探针与靶核酸反应，以在修饰的电极表面上形成杂交的核酸。杂交的核酸与氧化还原反应中能够氧化杂交核酸中的预选碱基的过渡金属络合物反应，检测氧化还原反应，核酸的存在或不存在从检测到氧化还原反应。

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