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41. 发明授权

US07537730B2 Reaction card and use of same 失效
标题翻译：反应卡和使用相同
公开(公告)号：US07537730B2
公开(公告)日：2009-05-26
申请号：US10362873
申请日：2001-08-27
申请人： Bruno Colin , Cécile Paris , Bernard Limon , Patrick Broyer , Charles Rogers
发明人： Bruno Colin , Cécile Paris , Bernard Limon , Patrick Broyer , Charles Rogers
IPC分类号： F16K35/00 , B01L11/00 , G01N21/00 , F17D1/16
CPC分类号： B01L3/502738 , B01L2200/10 , B01L2300/0816 , B01L2300/087 , B01L2400/0638 , B01L2400/0655 , G01N2035/00247 , G01N2035/00257 , Y10T137/0396
摘要： A reaction card (1) having a body, a front surface (62) and a rear surface (63) defined by an edge (68), at least one inlet (2, 3, 4 and/or 5) and at least one outlet (7and/or 8), connected by a network of channels (64) constituting at least one reaction path for at least one fluid which is directed via valves (11to 36 and/or 61); each valve includes a flexible film (67) which can be deformed to allow fluid passage, the film being fixed on the card's rear surface at a peripheral indentation of an assembly of channels associated with the valve; the card includes channels flush with at least one of its surfaces, the channels being of two different cross-sections, a small cross-section serving as a reaction compartment; each front or rear surface is delimited by at least one film (48, 49, 65, 66 and/or 67).
摘要翻译：具有主体的反应卡（1），由边缘（68）限定的前表面（62）和后表面（63），至少一个入口（2,3,4和/或5）和至少一个出口（7和/或8）通过构成用于至少一个通过阀（11至36和/或61）的流体的至少一个反应路径的通道网络（64）连接; 每个阀包括柔性膜（67），其可以变形以允许流体通过，所述膜被固定在卡的后表面上与阀相关联的通道组件的周边凹陷处; 卡包括与其表面中的至少一个平齐的通道，通道具有两个不同的横截面，小的横截面用作反应室; 每个前表面或后表面由至少一个膜（48,49,65,66和/或67）限定。

42. 发明申请

US20080041259A1 Pyrotechnic Microsystem and Method for Fabricating a Microsystem 失效
标题翻译：烟火微系统和制造微系统的方法
公开(公告)号：US20080041259A1
公开(公告)日：2008-02-21
申请号：US10585356
申请日：2005-01-20
申请人： Patrick Broyer , Bruno Colin , Denis Roller
发明人： Patrick Broyer , Bruno Colin , Denis Roller
IPC分类号： F42C19/12 , B05D1/02 , B05D1/18 , B05D1/36 , B32B27/00
CPC分类号： F16K99/0001 , F15B15/19 , F15C5/00 , F16K99/0015 , F16K99/0067 , F16K2099/008 , F42B3/04 , F42C19/06 , F42C19/12 , Y10T428/261 , Y10T428/31
摘要： The invention relates to a microsystem and a method for fabricating a microsystem. The pyrotechnic microsystem (7, 1′) comprises a substrate having at least two separate electrical initiation zones, each of which provides separate electrical initiation of a pyrotechnic material deposited on the substrate. This microsystem (7, 1′) is characterized in that the same pyrotechnic material deposit (721, 721′, 13) covers both initiation zones, said deposit (721, 721′, 13) produced on the substrate having a thickness sufficiently small for the initiation of the pyrotechnic material in the initiation zone to remain localized and not propagate to the other initiation zone, but sufficient to generate a specific gas quantity.
摘要翻译：本发明涉及微系统和微系统的制造方法。烟火微系统（7,1'）包括具有至少两个分开的电引发区的衬底，每个电引发区提供沉积在衬底上的烟火材料的单独的电引发。该微系统（7,1'）的特征在于，相同的烟火材料沉积物（721,721'，13）覆盖两个起始区域，所述沉积物（721,721'，13）在基板上产生的厚度足够小，起始区域中的烟火材料的起始保持局部化并且不会传播到另一个起始区，但足以产生特定的气体量。

43. 发明申请

US20050257829A1 Double-effect pyrotechnic micro-actuator for microsystem and microsystem using same 失效
标题翻译：用于微系统和微系统的双效烟火微致动器
公开(公告)号：US20050257829A1
公开(公告)日：2005-11-24
申请号：US10531947
申请日：2003-11-18
申请人： Patrick Broyer , Bruno Colin , Denis Roller
发明人： Patrick Broyer , Bruno Colin , Denis Roller
IPC分类号： B81B1/00 , B81B3/00 , F15C5/00 , F16K99/00 , H01H1/00 , H01H39/00 , F16K17/40
CPC分类号： F16K99/0001 , F15C5/00 , F16K99/0015 , F16K99/0067 , F16K2099/008 , H01H1/0036 , H01H39/00 , Y10T137/1647
摘要： The invention concerns the technical field of micro-actuators used for mechanical, chemical, electrical or thermal functions in microsystems, for microelectronic applications such as chips, or biomedical functions such as microfluidics integrating cards. the invention concerns a micro-actuator comprising a so-called main chamber, made in a solid support and containing a so-called main pyrotechnic charge, said main chamber being sealed and delimited by solid support walls and by a deformable membrane, such that the gases emitted by the combustion of the main pyrotechnic charge, enable the volume of said chamber to be increased by deforming said membrane while maintaining intact the solid walls of the main chamber. The invention is characterized in that it comprises means for evacuating the gases from the main chamber.
摘要翻译：本发明涉及用于微系统中的机械，化学，电或热功能的微致动器的技术领域，用于微电子应用如芯片或生物医学功能，例如集成卡的微流体。本发明涉及一种微致动器，其包括所谓的主室，其形成为固体支撑并且包含所谓的主要烟火充电，所述主室由实心支撑壁和可变形膜密封并界定，使得通过主烟火装置的燃烧发出的气体使得通过使所述膜变形而使所述室的体积增加，同时保持主室的固体壁完好无损。本发明的特征在于其包括用于从主室排出气体的装置。

44. 发明授权

US06632287B1 Method for decontaminating a hollow needle 失效
标题翻译：中空针头净化方法
公开(公告)号：US06632287B1
公开(公告)日：2003-10-14
申请号：US09529662
申请日：2000-04-18
申请人： Bruno Colin , Marie Privat
发明人： Bruno Colin , Marie Privat
IPC分类号： B08B702
CPC分类号： A61L2/04
摘要： The invention provides a method and device for decontaminating a contaminated hollow metal needle having electrical wires attached to the needle which heat cleaning fluid dispensed into the needle.
摘要翻译：本发明提供了一种用于净化污染的中空金属针的方法和装置，其具有附接到针上的电线，其中分配到针中的热清洁流体。

45. 发明授权

US06589790B1 Method and device for filling an analysis card with a liquid medium 失效
标题翻译：用液体介质填充分析卡的方法和装置
公开(公告)号：US06589790B1
公开(公告)日：2003-07-08
申请号：US09194726
申请日：1998-12-02
申请人： Bruno Colin , Cécile Jaravel
发明人： Bruno Colin , Cécile Jaravel
IPC分类号： C12M116
CPC分类号： B01L3/5025 , B01L3/50273 , B01L2200/027 , B01L2300/0816 , B01L2300/0864 , B01L2400/049 , G01N1/14 , G01N2001/2078 , G01N2035/00148 , Y10T436/111666 , Y10T436/119163 , Y10T436/25 , Y10T436/2575
摘要： Device for filling, with a liquid medium (1), an analysis card (2) comprising a body (3) in which at least one reading cavity (3a) is formed, and an orifice (4) communicating with the cavity via at least one internal channel (7), with an external conduit (9) communicating with the orifice, this device comprising an evacuation device (50). It comprises: a connecting device (11) including on one side a joining piece (12) for leaktight and removable coupling to the free end (9a) of the open external conduit, and communicating directly on the other side with the evacuation device (50); a device (34) for closing and re-opening the external conduit.
摘要翻译：用于用液体介质（1）填充分析卡（2）的装置，包括形成有至少一个读腔（3a）的主体（3），以及至少与空腔连通的孔口（4）一个内部通道（7），具有与孔口连通的外部导管（9），该装置包括排气装置（50）。它包括：一个连接装置（11），其一端包括接合件（12），用于密封和可拆卸地连接到开放的外部导管的自由端（9a），并且在另一侧与排气装置（50 ）; 用于关闭和重新打开外部导管的装置（34）。

46. 发明授权

US06263743B1 Liquid transferring device 失效
标题翻译：液体转移装置
公开(公告)号：US06263743B1
公开(公告)日：2001-07-24
申请号：US09202618
申请日：1998-12-18
申请人： Bruno Colin , Cécile Jaravel
发明人： Bruno Colin , Cécile Jaravel
IPC分类号： G01N100
CPC分类号： B01L3/0275 , G01N35/1095
摘要： A device for transferring a liquid sample drawn by suction, through a wall having an orifice, the device including a conduit, a plunger sealingly sliding in the conduit, and a rod controlling the plunger. The device includes a joining piece extending the conduit, with an outer cross-section adapted to fit in the aperture, for example sealing it, with close working clearance required for inserting the joining piece in the orifice, the joining piece being arranged to be separated from the conduit once it is engaged in the orifice, and to seal the orifice, when or once the joining piece engaged in the orifice is separated from the conduit.
摘要翻译：一种用于传送通过抽吸抽吸的液体样品的装置，通过具有孔口的壁，所述装置包括导管，在所述导管中密封地滑动的柱塞以及控制所述柱塞的杆。该装置包括延伸导管的接合件，其外部横截面适于安装在孔中，例如密封，具有将接合件插入孔中所需的紧密工作间隙，接合件被布置成分离从导管一旦接合在孔中并且密封孔口，何时或一旦接合在孔中的接合件与导管分离。

47. 发明授权

US6015531A Single-use analysis card comprising a liquid flow duct 失效
标题翻译：一次性分析卡，包括液体流动管道
公开(公告)号：US6015531A
公开(公告)日：2000-01-18
申请号：US983492
申请日：1998-03-05
申请人： Bruno Colin , Cecile Jaravel , Philippe Cleuziat
发明人： Bruno Colin , Cecile Jaravel , Philippe Cleuziat
IPC分类号： G01N33/543 , B01L3/00 , G01N35/02 , G01N21/00
CPC分类号： B01L3/502
摘要： The invention features an analysing device (1) comprising a body (2) in which are arranged or provided: an intake aperture (3) for a starting liquid sample, a liquid flow circuit (5) comprising at least one operating cell (6) for a processed liquid sample, obtained from all or part of the original sample, communicating with the said intake aperture (3), the said flow circuit defining, in at least two dimensions of the card, one determined geometric line, such that any alteration in the card orientation in a three-dimensional reference frame, causes the liquid to flow under gravity only, from one part of the said circuit to another,for instance from one side or another of the operating cell, (6) characterised in that, the flow circuit (5) is continuous, and looped on itself between the said aperture (3) and the said operating cell (6).
摘要翻译： PCT No.PCT / FR97 / 01020 Sec。 371日期：1998年3月5日 102（e）1998年3月5日PCT 1997年6月9日PCT公布。 WO97 / 46318 PCT公开号日期1997年12月11日本发明的特征在于一种分析装置（1），包括其中布置或提供的主体（2）;用于起始液体样品的进气孔（3），包括至少一个从所述原始样品的全部或部分获得的用于经处理的液体样品的操作室（6）与所述进气孔（3）连通，所述流动回路在卡的至少两个维度上限定一个确定的几何线，使得三维参考系中的卡方向的任何改变使得液体仅在重力作用下从所述电路的一部分流向另一部分，例如来自操作单元的一侧或另一侧（6 ），其特征在于，流动回路（5）是连续的，并且在所述孔（3）和所述操作单元（6）之间自身环绕。

48. 发明授权

US5773307A Method and device for determining an analyte in a sample 失效
标题翻译：用于测定样品中分析物的方法和装置
公开(公告)号：US5773307A
公开(公告)日：1998-06-30
申请号：US615204
申请日：1996-03-11
申请人： Bruno Colin , Michel Goudard , Alain Theretz
发明人： Bruno Colin , Michel Goudard , Alain Theretz
IPC分类号： G01N33/543 , G01N33/553 , B03C1/00 , C12M1/40 , G01N33/544
CPC分类号： G01N33/54366 , G01N33/54333 , G01N33/553 , Y10S436/806
摘要： A method for determining an analyte in a sample which includes: providing the sample in liquid phase, the analyte of which has a ligand having at least one anti-ligand specific recognition site; providing at least one reagent containing metal particles, in suspension in liquid phase and non-separable from the liquid phase by a magnetic or electromagnetic force, each particle containing a metal core to which at least one anti-ligand is fixed directly or indirectly; contacting the sample and the at least one reagent in order to obtain metal clusters; placing the metal clusters in a magnetic field generated by a magnetic or magnetic field generating sensor in order to assemble the metal clusters; and detecting the assembled metal clusters to obtain a signal representative of the mass of metal in the clusters, which is correlated with the quantity of analyte present in the sample initially. A device for determining an analyte in a sample by the above process is also included.
摘要翻译： PCT No.PCT / FR94 / 01099 Sec。 371日期：1996年3月11日 102（e）1996年3月11日PCT PCT 1994年9月20日PCT公布。公开号WO95 / 08769 日期1995年3月30日一种用于测定样品中分析物的方法，其包括：提供液相中的样品，其分析物具有至少一个抗配体特异性识别位点的配体; 提供至少一种含有金属颗粒的试剂，其以液相悬浮并且通过磁力或电磁力不能与液相分离，每个颗粒含有至少一种抗配体直接或间接固定的金属核; 使样品和至少一种试剂接触以获得金属簇; 将金属簇放置在由磁场或磁场产生传感器产生的磁场中，以便组装金属簇; 并检测组合的金属簇以获得代表簇中金属质量的信号，其与初始样品中存在的分析物的量相关。还包括用于通过上述方法确定样品中的分析物的装置。

49. 发明授权

US5765490A Device for withdrawing and/or ejecting a contaminating medium, with decontamination of the member for manipulating said medium 失效
标题翻译：用于抽出和/或喷射污染介质的装置，用于对用于操纵所述介质的构件进行去污染
公开(公告)号：US5765490A
公开(公告)日：1998-06-16
申请号：US600845
申请日：1996-02-13
申请人： Bruno Colin , Genevieve Bossy
发明人： Bruno Colin , Genevieve Bossy
IPC分类号： G01N1/28 , A61L2/04 , G01N1/34 , G01N35/00 , G01N35/10 , F23G5/00
CPC分类号： A61L2/04 , G01N35/1004 , G01N2035/00425 , G01N2035/1086
摘要： Process for heating a hollow needle (2) consisting essentially of a metal tube (3), the inside and/or outside of which have previously been brought into contact with a contaminating medium, according to which an electric current is supplied to the said needle, flowing in the metal wall and over the length of the said tube (3) and dissipating thermal energy therein through the ohmic effect, characterized in that a predetermined quantity of electrical energy is supplied to the said needle (2), metered as a function of the electrical characteristics of the said tube (3), on the one hand to suffice for decontamination of the needle, both inside and outside, and on the other hand to limit the heating and preserve the integrity, including the original shape, of the said needle.
摘要翻译：用于加热基本上由金属管（3）组成的中空针（2）的方法，其中内部和/或外部已经与污染介质接触，根据该中空针将电流提供给所述针在金属壁中流动并超过所述管（3）的长度并通过欧姆效应在其中消散热能，其特征在于，将预定量的电能供应到所述针（2），作为功能计量所述管（3）的电气特性一方面足以对内部和外部的针进行去污，另一方面限制加热并保持包括原始形状在内的完整性说针。

50. 发明申请

US20110275110A1 METHOD FOR ISOLATING OR COUNTING MICROORGANISMS ON AN AGAR CULTURE MEDIUM 审中-公开
标题翻译：在小麦培养基中分离或计数微生物的方法
公开(公告)号：US20110275110A1
公开(公告)日：2011-11-10
申请号：US13143879
申请日：2010-01-29
申请人： Bruno Colin , Aurelien Costa
发明人： Bruno Colin , Aurelien Costa
IPC分类号： C12Q1/24 , C12N1/00
CPC分类号： C12Q1/04
摘要： The invention relates to a method for isolating microorganisms on an agar culture medium, including the steps of: depositing, on said agar culture medium, a predetermined amount of a sample to be analyzed and optionally containing said microorganisms or a suspension of said microorganisms; applying a seeding means onto agar culture medium in contact with the volume of sample or suspension; moving the seeding means so as totally or partially spread the volume of sample or suspension over the surface of the agar culture medium, the movement of said seeding means being discontinuous so that during said movement the contact between the seeding means and the surface of the agar culture medium is interrupted and re-established at least once, thereby creating spreading segments and resulting in a depletion of the seeding means in terms of the sample or suspension; and incubating said agar culture medium under conditions enabling the growth of microorganisms.
摘要翻译：本发明涉及一种用于在琼脂培养基上分离微生物的方法，包括以下步骤：在所述琼脂培养基上沉积预定量的待分析样品，任选地含有所述微生物或所述微生物的悬浮液; 在接种体积的样品或悬浮液的琼脂培养基上施用播种装置; 移动接种装置以使样品或悬浮液的体积完全或部分地扩散到琼脂培养基的表面上，所述接种装置的移动是不连续的，使得在所述移动期间，接种装置和琼脂表面之间的接触培养基被中断并重新建立至少一次，从而产生扩展段并导致播种装置在样品或悬浮液方面的消耗; 并在允许微生物生长的条件下培养所述琼脂培养基。

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