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41. 发明授权

US07033981B2 Alkaline cellulase variants 有权
标题翻译：碱性纤维素酶变体
公开(公告)号：US07033981B2
公开(公告)日：2006-04-25
申请号：US10383630
申请日：2003-03-10
申请人： Yoshihiro Hakamada , Kazuhisa Sawada , Keiji Endo , Hiroshi Kodama , Yasunao Wada , Shitsu Shikata , Tohru Kobayashi
发明人： Yoshihiro Hakamada , Kazuhisa Sawada , Keiji Endo , Hiroshi Kodama , Yasunao Wada , Shitsu Shikata , Tohru Kobayashi
IPC分类号： C11D3/386 , C12N9/42 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004
摘要： The present invention relates to alkaline cellulase variants having an amino acid sequence which exhibits at least 95% homology with the amino acid sequence of SEQ ID NO: 2, wherein said alkaline cellulase variant is mutated to include at least one substitution at (a) position 10, (b) position 16, (c) position 22, (d) position 33, (e) position 39, (f) position 76, (g) position 109, (h) position 242, (i) position 263, (j) position 308, (k) position 462, (l) position 466, (m) position 468, (n) position 552, (o) position 564, or (p) position 608 in SEQ ID NO: 2, or at a position corresponding thereto, wherein said alkaline cellulase variant has alkaline cellulase activity. The present invention also relates to a gene encoding the variant; a vector containing the gene; a transformant containing the vector; and a detergent composition containing the alkaline cellulase variant.
摘要翻译：本发明涉及具有与SEQ ID NO：2的氨基酸序列具有至少95％同源性的氨基酸序列的碱性纤维素酶变体，其中所述碱性纤维素酶变体突变以在（a）位置包括至少一个取代 10，（b）位置16，（c）位置22，（d）位置33，（e）位置39，（f）位置76，（g）位置109，（h）位置242，（i）位置263，（j）位置308，（k）SEQ ID NO：2中的位置462，（l）位置466，（m）位置468，（n）位置552，（o）位置564或（p）位置608，或在相应的位置，其中所述碱性纤维素酶变体具有碱性纤维素酶活性。本发明还涉及编码该变体的基因; 含有该基因的载体; 含有载体的转化体和含有碱性纤维素酶变体的洗涤剂组合物。

42. 发明授权

US4659365A Herbicidal 2-chloro-2'-fluoroalkoxyacetanilides 失效
标题翻译：除草剂2-氯-2'-氟代烷氧基乙酰苯胺
公开(公告)号：US4659365A
公开(公告)日：1987-04-21
申请号：US769123
申请日：1985-08-26
申请人： Kazuhiko Konno , Koichi Araki , Kaoru Ikeda , Keiji Endo , Mitsuru Hikido
发明人： Kazuhiko Konno , Koichi Araki , Kaoru Ikeda , Keiji Endo , Mitsuru Hikido
IPC分类号： A01N37/24 , A01N37/26 , A01N43/56 , C07C233/88 , C07D231/12 , C07D521/00 , C07C103/375
CPC分类号： C07D231/12 , A01N37/24 , A01N37/26 , A01N43/56 , C07D233/56 , C07D249/08
摘要： An acetanilide derivative of the following formula (I) and a herbicide containing one or more of the acetanilide derivative as the active ingredient are described. ##STR1## In the formula, R.sup.1 is a lower alkyl group; R.sup.2 is a fluorine-substituted lower alkyl group; A is ##STR2## B is an optionally substituted pyrazolyl group, an optionally substituted alkoxy group or a hydrogen atom.The acetanilide derivative and the herbicide have a selective weed killing activity to gramiveous weeds.
摘要翻译：描述下式（I）的乙酰苯胺衍生物和含有一种或多种乙酰苯胺衍生物作为活性成分的除草剂。在该式中，R 1为低级烷基; R2是氟取代的低级烷基; A是B是任选取代的吡唑基，任选取代的烷氧基或氢原子。乙酰苯胺衍生物和除草剂对杂草杂草具有选择性除草活性。

43. 发明授权

US09169458B2 Mutated alkaline cellulase 有权
标题翻译：突变碱性纤维素酶
公开(公告)号：US09169458B2
公开(公告)日：2015-10-27
申请号：US13583508
申请日：2011-03-11
申请人： Ayako Shimazu , Keiji Endo , Mitsuyoshi Okuda
发明人： Ayako Shimazu , Keiji Endo , Mitsuyoshi Okuda
IPC分类号： C12N9/42 , C12N1/20 , C12N15/00 , C07H21/04 , C11D3/386
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention provides a mutant alkaline cellulase having enhanced anti-redeposition ability. The present invention provides a mutant alkaline cellulase having an amino acid sequence in which at least amino acid residue selected from the amino acid residues at the positions corresponding to positions 58, 45, 52, 56, 60, 64, 66, 71, 103, 119, 122, 123, 124, 125, 127, 130, 140, 161, 164, 175, 176, 178, 179, 181, 193, 194, 195, 196, 197, 199, 202, 203, 217, 225, 227, 228, 251, 267, 272, 276, 277, 280, 282, 297, 310, 312, 318, 324, 345, 354, 356, 357, 360, 363, 368, 418, 419, 420, 421, 422, 453, 454, 455, 457, 458, 459, 494, 495, 496, 500, 501, 502, 503, 504, 550, 551, 552, 604, 605, 606, 607, 608, 640, 641, 642, 644, 645, 646, 683, 684, 685, 690, 691, 692, 693, 694, 739, 740, and 741 of the amino acid sequence of an alkaline cellulase set forth in SEQ ID NO: 2 or of the amino acid sequence of an alkaline cellulase having at least 70% identity with the amino acid sequence of SEQ ID NO: 2, is substituted with other amino acid residue.
摘要翻译：本发明提供具有增强的抗再沉积能力的突变型碱性纤维素酶。本发明提供了一种具有氨基酸序列的突变型碱性纤维素酶，其中至少选自氨基酸残基的氨基酸残基位于对应于位置58,45,52,56,66,66,61,103,60,64,66,67,60,62,68,62,62,64,68,62,62,62,67,67,69,68,62,62,67 119,122,123,124,125,127,130,140,161,164,175,176,178,179,181,193,194,195,196,197,199,202,203,217,225， 227,228,251,267,272,276,277,280,282,277,310,312,318,324,345,354,356,357,360,363,368,418,419,420,421,418,417,318,472,427,268,267,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282 422,453,454,455,457,458,459,494,495,496,5500,501,502,503,504,550,551,552,604,605,606,607,608,640,641，或SEQ ID NO：2所示的碱性纤维素酶的氨基酸序列的642,644,645,646,683,684,685,690,691,692,693,694,739,740和741，或与SEQ ID NO：2的氨基酸序列具有至少70％同一性的碱性纤维素酶的氨基酸序列被其它氨基酸残基取代。

44. 发明申请

US20130219557A1 Method of Producing Fatty Acids or Lipids Containing Fatty Acids Using Thioesterase Variants 有权
标题翻译：使用硫酯酶变体生产含有脂肪酸的脂肪酸或脂质的方法
公开(公告)号：US20130219557A1
公开(公告)日：2013-08-22
申请号：US13516047
申请日：2010-12-03
申请人： Takuto Tojo , Keiji Endo
发明人： Takuto Tojo , Keiji Endo
IPC分类号： C12N15/82
CPC分类号： C12N15/8247 , C07K14/415 , C12N9/16 , C12N15/8218 , C12N15/8285 , C12P7/6463
摘要： A method of producing a fatty acid or a lipid containing a fatty acid, using a thioesterase variant having an amino acid sequence in which the 231st amino acid is substituted from threonine to lysine in the amino acid sequence set forth in SEQ ID NO: 1; and a transformant obtained by introducing a gene that encodes the thioesterase variant which transformant has an enhanced ability to produce a fatty acid or a lipid containing a fatty acid.
摘要翻译：使用具有SEQ ID NO：1所示氨基酸序列中第231位氨基酸被苏氨酸取代为赖氨酸的氨基酸序列的硫酯酶变体的脂肪酸或含脂肪酸的脂质的制造方法; 以及通过引入编码转化体的硫酯酶变体的基因获得的转化体具有增强的产生脂肪酸的能力或含有脂肪酸的脂质的能力。

45. 发明申请

US20130059351A1 Thioesterase and a Method of Producing Fatty Acids or Lipids Using the Thioesterase 有权
标题翻译：硫酯酶和使用硫酯酶生产脂肪酸或脂质的方法
公开(公告)号：US20130059351A1
公开(公告)日：2013-03-07
申请号：US13696147
申请日：2011-04-13
申请人： Takuto Tojo , Keiji Endo
发明人： Takuto Tojo , Keiji Endo
IPC分类号： C12N9/16 , C12N1/21 , C12N15/55 , C12P7/64 , C12N15/70 , C12N15/82 , C12N15/63 , C12N5/10
CPC分类号： C12N15/8247 , C12N9/16 , C12P7/6418 , C12P7/6436 , C12Y301/02014
摘要： A thioesterase comprising an amino acid sequence set forth in SEQ ID NO: 1, a thioesterase gene encoding the thioesterase, a transformant comprising the gene, and a method of producing fatty acids or lipids using the transformant.
摘要翻译：包含SEQ ID NO：1所示氨基酸序列的硫酯酶，编码硫酯酶的硫酯酶基因，包含该基因的转化体，以及使用该转化体产生脂肪酸或脂质的方法。

46. 发明授权

US08389264B2 Recombinant microorganism that expresses a secY gene with deletion of sporulation-associated genes and method of producing thereof 有权
标题翻译：表达具有孢子形成相关基因缺失的secY基因的重组微生物及其制备方法
公开(公告)号：US08389264B2
公开(公告)日：2013-03-05
申请号：US12530135
申请日：2008-04-08
申请人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
发明人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
IPC分类号： C12N1/21
CPC分类号： C12P21/02
摘要： A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided.A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.
摘要翻译：提供了具有提高的蛋白质或多肽的生产力的重组微生物，以及使用该重组微生物生产蛋白质或多肽的方法。将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物，以及删除或灭活所选择的一种或多种基因来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。

47. 发明授权

US07855065B2 Mutant bacterium belonging to the genus Bacillus 有权
标题翻译：属于芽孢杆菌属的突变细菌
公开(公告)号：US07855065B2
公开(公告)日：2010-12-21
申请号：US10590275
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
IPC分类号： C12N1/20 , C12N15/74 , C12N15/75 , C12P21/04
CPC分类号： C12N9/2417 , C12N9/2437 , C12N9/54 , C12Y302/01004
摘要： The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism. A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.
摘要翻译：本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌，通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物，以及通过使用重组微生物产生蛋白质或多肽的方法。一种突变芽孢杆菌属细菌，其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA，重组微生物通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生，以及通过使用重组微生物产生蛋白质或多肽的方法。

48. 发明授权

US07829703B2 Difluoromethanesulfonyl anilide derivatives useful as herbicides 有权
标题翻译：用作除草剂的二氟甲磺酰苯胺衍生物
公开(公告)号：US07829703B2
公开(公告)日：2010-11-09
申请号：US12190703
申请日：2008-08-13
申请人： Koichi Araki , Sachio Kudo , Yoshitaka Sato , Keiji Endo , Shinichi Shirakura , Shin Nakamura
发明人： Koichi Araki , Sachio Kudo , Yoshitaka Sato , Keiji Endo , Shinichi Shirakura , Shin Nakamura
IPC分类号： C07D251/20 , A01N43/66 , C07D239/52 , A01N43/54
CPC分类号： C07D239/52
摘要： Novel difluoromethanesulfonamide derivatives of the formula (I) Difluoromethanesulfonamide derivatives represented by the formula wherein X represents halogen, Y represents CH or N, R1 represents hydrogen, and R2 represents hydrogen or hydroxy, or R1 and R2 together may form C═O with the carbon atom to which they are bonded, and their use as herbicide for paddy field weeds.
摘要翻译：式（I）的新型二氟甲磺酰胺衍生物由下式表示的二氟甲磺酰胺衍生物其中X表示卤素，Y表示CH或N，R1表示氢，R2表示氢或羟基，或R1和R2可以与碳形成C = O 它们所连接的原子，以及它们作为水田杂草除草剂的用途。

49. 发明授权

US07585674B2 Host microorganisms 失效
标题翻译：宿主微生物
公开(公告)号：US07585674B2
公开(公告)日：2009-09-08
申请号：US10479214
申请日：2002-05-28
申请人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
发明人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
IPC分类号： C12N5/06 , G01N33/53
CPC分类号： C07K14/32 , C07K2319/00 , C12N1/20
摘要： A microorganism wherein one or more genes selected from the group of genes participating in sporulation in the middle to late stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) by use the microorganism. No spore is formed when this microorganism is employed, thereby enabling production of a target product (a protein) while decreasing energy loss, production of a by-product and specific production speed to decrease unnecessary consumption of a medium. Moreover, the production period can be prolonged, whereby the target product (the protein) can be produced efficiently.
摘要翻译：一种微生物，其中一个或多个选自参与孢子形成中期至晚期孢子形成的基因的基因已被删除或失活; 以及通过使用微生物生产目标产物（蛋白质）的方法。当使用该微生物时不形成孢子，从而能够减少能量损失，副产物的产生和特定的生产速度，从而能够生产目标产物（蛋白质），从而减少不必要的培养基消耗。此外，可以延长生产周期，由此有效地生产目标产物（蛋白质）。

50. 发明申请

US20090062536A1 USE OF SULFONANILIDE COMPOUNDS AS HERBICIDE 审中-公开
标题翻译：磺酰亚胺化合物用作除草剂
公开(公告)号：US20090062536A1
公开(公告)日：2009-03-05
申请号：US12193236
申请日：2008-08-18
申请人： KOICHI ARAKI , Yoshitaka Sato , Shinichi Shirakura , Keiji Endo , Shin Nakamura , Seiji Ukawa , Chieko Ueno
发明人： KOICHI ARAKI , Yoshitaka Sato , Shinichi Shirakura , Keiji Endo , Shin Nakamura , Seiji Ukawa , Chieko Ueno
IPC分类号： C07D251/30 , C07D239/48
CPC分类号： C07D239/52 , A01N43/54 , A01N43/66 , C07D239/34 , C07D251/26
摘要： A herbicide comprising as an active ingredient a sulfonanilide compound represented by the formula: wherein, R1 represents CHF2 or CH2CF3; R2 represents hydrogen, C1-3 alkyl, 3-propenyl, or 3-propynyl; R3 represents hydrogen; R4 represents hydrogen, hydroxy, or methylthio, or R3 and R4, together with a carbon atom to which they are bonded, may form C═O; R5 represents halogen or methyl; X represents methoxy or chlorine; and Z represents CH or N; with the proviso that, (i) when R1 represents CH2CF3, R2 represents hydrogen, R5 represents bromine or iodine, X represents methoxy, and Z represents CH, (ii) when R1 represents CHF2 and X represents methoxy, R5 represents bromine or iodine, Z represents N, and R2 represents C1-3 alkyl, 3-propenyl, or 3-propynyl, (iii) when R1 represents CHF2 and X represents chlorine, Z represents CH.
摘要翻译：一种除草剂，其包含由下式表示的磺酰苯胺化合物作为活性成分：其中，R 1表示CHF 2或CH 2 CF 3; R 2表示氢，C 1-3烷基，3-丙烯基或3-丙炔基; R3表示氢; R4表示氢，羟基或甲硫基，或R3和R4与它们所键合的碳原子一起形成C-O; R5代表卤素或甲基; X表示甲氧基或氯; Z表示CH或N; 条件是（i）当R 1表示CH 2 CF 3时，R 2表示氢，R 5表示溴或碘，X表示甲氧基，Z表示CH，（ii）当R 1表示CHF 2且X表示甲氧基时，R 5表示溴或碘， Z表示N，R2表示C1-3烷基，3-丙烯基或3-丙炔基，（iii）当R1表示CHF2且X表示氯时，Z表示CH。

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