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    • 43. 发明授权
    • Systems and methods for cryopreservation of cells
    • 用于细胞冷冻保存的系统和方法
    • US07939316B2
    • 2011-05-10
    • US11765000
    • 2007-06-19
    • Erik John WoodsJohn K. Critser
    • Erik John WoodsJohn K. Critser
    • C12M1/12C12M3/06
    • A01N1/02A01N1/0263A01N1/0268A01N1/0278
    • An auto-nucleating device includes a hollow tube containing a crystalline cholesterol matrix therein. The ends of the tube are closed by a membrane that is impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto-nucleating device is disposed within the vessel and provides a site for ice nucleation during freezing of the liquid within the vessel. One such cryopreservation vessel is a flexible vial having a closed port at one adapted to be pierced by a needle to withdraw the liquid within. The opposite end of the vial is initially open to receive the liquid. The end is then sealed to form a closed system for cryopreservation. Another cryopreservation vessel includes an adaptor mounted to a port of a liquid container. The adaptor includes one tubular branch that is closed by a needle septum while another tubular branch includes a barbed fitting for engaging a flexible tube. The flexible term terminates in a needle septum. The container is initially at below-atmospheric pressure. In use, the liquid sample is injected by needle through the septum in the second branch. Once the container is filled with the sample, the flexible tube is heat sealed and severed just above the barbed fitting. The sealed container may then be subject to a cryopreservation protocol. After thawing, the sample liquid may be withdrawn by a needle puncturing the septum in the first branch.
    • 自动成核装置包括其中含有结晶胆固醇基质的中空管。 管的端部由对胆固醇不渗透但对包含在冷冻保存容器中的液体可渗透的膜封闭。 自动成核装置设置在容器内并且在冷冻容器内的液体时提供用于冰成核的部位。 一个这样的冷冻保存容器是柔性小瓶,其具有适于被针刺穿的一个封闭端口,以抽出其中的液体。 小瓶的相对端最初开放以接收液体。 然后将其末端密封以形成用于低温保存的封闭系统。 另一个冷冻保存容器包括安装到液体容器的端口的适配器。 适配器包括一个由针隔膜封闭的管状分支,而另一个管状分支包括用于接合柔性管的带倒钩的配件。 灵活的术语终止于针间隔。 容器最初处于低于大气压。 在使用中,液体样品通过针头穿过第二分支中的隔膜。 一旦容器充满样品,柔性管被热封并刚好在倒钩配件上方切断。 密封容器然后可以进行冷冻保存方案。 解冻后,样品液体可以被穿刺在第一分支中的隔膜的针抽出。
    • 47. 发明授权
    • Collapsible stage or like structure
    • 可折叠阶段或类似结构
    • US06195943B1
    • 2001-03-06
    • US09367279
    • 1999-08-16
    • Carl Henry WoodsDavid John WoodsRushton Carl Woods
    • Carl Henry WoodsDavid John WoodsRushton Carl Woods
    • E04H326
    • E04H3/28
    • A collapsible stage (1) is provided which has a first planar wing (3), a second planar wing (5) and an intermediate planar section (7). The respective wings (3 to 5) are hinged to respective sides of the intermediate section (7) so they can both swing upwardly from a plane where they are co-planar with the intermediate section where they collectively form an open stage (1), to a collapsed position where both wings (3 to 5) are generally parallel to one another and extend upright. The intermediate section (7) has ground engaging devices (17) to permit rolling or sliding movement of the collapsed stage. A first pair of retractable legs (11) are connected with an underside of the first wing (3) adjacent one side of the intermediate section (7), and a second pair of retractable legs (13) are connected with an underside of the second wing (5) and the other side of the intermediate section (7). These legs (11 and 13) are guided by guide devices (21) attached to the intermediate section (7) so that feet on those legs will be below the ground engaging devices (17) when the wings (3 and 5) are swung so they are substantially co-planar with the intermediate section (7), and will be above the ground engaging devices (17) when the wings (3 and 5) are swung upwardly to the collapsed position. The outermost ends of the wings (3 and 5) each have further legs (35) swingably connected thereto which can be swung from a collapsed position where they extend substantially parallel to the planes of the respective wings (3 and 5) to erect positions where they are substantially perpendicular to the planes of the wings (3 and 5).
    • 提供具有第一平面翼(3),第二平面翼(5)和中间平面部分(7)的可折叠台(1)。 相应的翼(3至5)铰接到中间部分(7)的相应侧面,使得它们可以从它们与共同形成开放平台(1)的中间部分共面的平面向上摆动, 到两个翼(3至5)大致彼此平行并且直立延伸的折叠位置。 中间部分(7)具有地面接合装置(17),以允许塌陷阶段的滚动或滑动运动。 第一对可伸缩腿部(11)与第一翼片(3)的与中间部分(7)的一侧相邻的下侧连接,第二对可缩回的腿部(13)与第二翼片 翼部(5)和中间部分(7)的另一侧。 这些腿(11和13)由附接到中间部分(7)的引导装置(21)引导,使得当翼(3和5)摆动时,这些腿上的脚将落在地面接合装置(17)的下面 它们与中间部分(7)基本上共面,并且当翼(3和5)向上摆动到折叠位置时,它们将在地面接合装置(17)的上方。 机翼(3和5)的最外端各自具有可摆动地连接到其上的另外的腿部(35),其可以从折叠位置摆动,其中它们基本上平行于各个翼部(3和5)的平面延伸,以竖立位置, 它们基本上垂直于翼(3和5)的平面。