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31. 发明授权

US06635782B2 Polyhydroxyalkanoate and manufacturing method thereof 失效
标题翻译：聚羟基链烷酸酯及其制造方法
公开(公告)号：US06635782B2
公开(公告)日：2003-10-21
申请号：US09951720
申请日：2001-09-14
申请人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Takashi Kenmoku
发明人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Takashi Kenmoku
IPC分类号： C07C6976
CPC分类号： C12P7/625 , C08G63/06 , C08G63/6822 , C08G63/6852
摘要： To provide a novel polyhydroxyalkanoate and a manufacturing method by a microorganism capable of substantially reducing unintended monomer units and obtaining the polyhydroxyalkanoate in a high yield. A microorganism capable of synthesizing a novel polyhydroxyalkanoate having 3-hydroxy-substituted benzoylalkanoic acid as a monomer unit using a substituted benzoylalkanoic acid as a material is cultured in the medium containing a substituted benzoylalkanoic acid, then the polyhydroxyalkanoate produced in the cultured bacteria is extracted and recovered.
摘要翻译：提供一种新型的聚羟基链烷酸酯及其制造方法，该微生物能够基本上减少非预期的单体单元并以高产率获得聚羟基链烷酸酯。在含有取代的苯甲酰基链烷酸的培养基中培养能够使用取代的苯甲酰基链烷酸合成具有3-羟基取代的苯甲酰基链烷酸作为单体单元的新型聚羟基链烷酸酯的微生物，然后提取培养细菌中产生的聚羟基链烷酸酯，恢复。

32. 发明申请

US20080108123A1 MAGNETIC SUBSTANCE-BIOSUBSTANCE COMPLEX STRUCTURE, PEPTIDE FRAGMENT CAPABLE OF LINKING TO MAGNETIC SUBSTANCE AND GENE THEREFOR, AND PROCESS FOR PRODUCING THE COMPLEX STRUCTURE 审中-公开
标题翻译：磁性物质 - 生物传感器复合结构，能够连接到磁性物质及其基因的肽片段，以及用于生产复合结构的方法
公开(公告)号：US20080108123A1
公开(公告)日：2008-05-08
申请号：US11937611
申请日：2007-11-09
申请人： Takeshi Imamura , Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Tsutomu Honma , Akiko Tsuchitani
发明人： Takeshi Imamura , Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Tsutomu Honma , Akiko Tsuchitani
IPC分类号： C12N9/00 , C07H23/00 , C11C3/00 , C07K1/00 , C07H21/04 , C07K14/00
CPC分类号： C12N9/1025 , C07K2319/00 , C12N11/06 , C12N11/14 , C12N15/1013 , G01N33/54326 , G01N33/54353 , Y10S530/811
摘要： A magnetic substance-biosubstance complex structure comprises a magnetic substance-containing carrier and a biosubstance immobilized on the carrier, the biosubstance being immobilized through a spacer comprising an amino acid sequence on a surface of the carrier.
摘要翻译：磁性物质 - 生物物质复合物结构包括含磁性物质的载体和固定在载体上的生物体，生物质通过包含载体表面上的氨基酸序列的间隔物固定化。

33. 发明授权

US07527809B2 Polyhydroxyalkanoate-containing magnetic structure, and manufacturing method and use thereof 失效
标题翻译：含聚羟基链烷酸酯的磁性结构体及其制造方法和用途
公开(公告)号：US07527809B2
公开(公告)日：2009-05-05
申请号：US10553221
申请日：2004-04-30
申请人： Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Takeshi Imamura , Tsutomu Honma
发明人： Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Takeshi Imamura , Tsutomu Honma
IPC分类号： A61K9/14 , A61K9/50
CPC分类号： A61K9/5094 , A61K49/226 , Y10T428/12014
摘要： A structure containing polyhydroxyalkanoate and a magnetic substance includes an external phase part containing polyhydroxyalkanoate and an internal phase part contained in the external phase part, at least one of the external phase part and the internal phase part containing a magnetic substance. Accordingly, provided is a microcapsule, which can be suitably used for formation of an active ingredient of a sustained release pharmaceutical preparation or an ultrasonic contrast agent.
摘要翻译：包含聚羟基链烷酸酯和磁性物质的结构包括含有聚羟基链烷酸酯的外相部分和包含在外部相部分中的内相部分，外相部分和内相部分中的至少一个含有磁性物质。因此，提供了一种微胶囊，其可适用于形成持续释放药物制剂或超声造影剂的活性成分。

34. 发明申请

US20070003975A1 Structured construct and producing method therefor 审中-公开
标题翻译：结构构造及其制造方法
公开(公告)号：US20070003975A1
公开(公告)日：2007-01-04
申请号：US10544942
申请日：2004-05-06
申请人： Takeshi Imamura , Tetsuya Yano , Tsutomu Honma , Shinya Kozaki , Tsuyoshi Nomoto , Akiko Tsuchitani
发明人： Takeshi Imamura , Tetsuya Yano , Tsutomu Honma , Shinya Kozaki , Tsuyoshi Nomoto , Akiko Tsuchitani
IPC分类号： C12P7/62 , G01N33/53 , B32B27/14
CPC分类号： C12N9/93 , C07K2319/23 , C09D167/04 , C12N11/08 , C12P7/625 , G01N33/54326 , G01N33/54393 , G01N2333/21 , G01N2333/245
摘要： There is provided a construct in which at least a part of the magnetic material is coated with a polyhydroxyalkanoate (PHA), and a method for producing a construct by immobilizing a PHA synthesizing enzyme on the surface of the magnetic material, thereby biosynthesizing and coating a PHA.
摘要翻译：提供了其中至少一部分磁性材料涂覆有聚羟基链烷酸酯（PHA）的构造，以及通过将PHA合成酶固定在磁性材料的表面上来生产构建体的方法，从而生物合成和涂覆 PHA。

35. 发明授权

US07045321B2 POLYHYDROXYALKANOATE CONTAINING UNIT WITH PHENYLSULFANYL STRUCTURE IN THE SIDE CHAIN, PROCESS FOR ITS PRODUCTION, CHARGE CONTROL AGENT, TONER BINDER AND TONER WHICH CONTAIN NOVEL POLYHYDROXYALKANOATE, AND IMAGE-FORMING METHOD AND IMAGE-FORMING APPARATUS WHICH MAKE USE OF THE TONER 失效
标题翻译：含有侧链中的酚醛结构的聚羟基烷酸酯单体，其生产方法，充电控制剂，含有新型聚羟基烷基酯的调色剂和调色剂，以及使用该调色剂的图像形成方法和成像装置
公开(公告)号：US07045321B2
公开(公告)日：2006-05-16
申请号：US10084172
申请日：2002-02-28
申请人： Takeshi Imamura , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Tomohiro Suzuki , Tsutomu Honma , Takashi Kenmoku , Tatsuki Fukui
发明人： Takeshi Imamura , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Tomohiro Suzuki , Tsutomu Honma , Takashi Kenmoku , Tatsuki Fukui
IPC分类号： C12P11/00 , C12P13/00 , C08G63/02
CPC分类号： G03G9/08755 , C08G63/6882 , C12P7/625 , C12P11/00 , G03G9/08791 , G03G9/09733 , G03G9/09775
摘要： A polyhydroxyalkanoate characterized by having in the molecule a unit represented by Chemical Formula (1): wherein n may assume any one integral value within the range of from 1 to 8. Also disclosed are a process for producing the polyhydroxyalkanoate by the use of a microorganism having the ability to produce the polyhydroxyalkanoate and accumulate it in the bacterial body; a charge control agent, a toner binder and a toner which contain this polyhydroxyalkanoate; and an image-forming method and an image-forming apparatus which make use of the toner.
摘要翻译：公开了在分子中具有由化学式（1）表示的单元的聚羟基链烷酸酯。其中R选自氢原子，卤原子，CN，NO 2，COOR'，SO 2 R“，CH 3，C 2 H 5，C 3 H 7，C（CH 3）2 H和C（CH 3）3; 其中R'是H，Na，K，CH3或C2H5，R“是OH，ONa，OK，卤素原子，OCH3或OC2H5; x是任意选自1至8的整数; 条件是排除了具有氢原子作为R的聚羟基链烷酸酯，并且所有单元中的x均为2或4.还公开了通过使用具有生产聚羟基链烷酸酯的能力的微生物来生产聚羟基链烷酸酯并累积它在细菌体内

36. 发明授权

US06911521B2 Polyhydroxyalkanoate that comprises unit having substituted or unsubstituted (phenylmethyl) sulfanyl structure in side chain thereof and process for producing the same 失效
标题翻译：包含在侧链具有取代或未取代（苯基甲基）硫烷基结构的单元的聚羟基链烷酸酯及其制备方法
公开(公告)号：US06911521B2
公开(公告)日：2005-06-28
申请号：US10157142
申请日：2002-05-30
申请人： Takashi Kenmoku , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Tsutomu Honma
发明人： Takashi Kenmoku , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Tsutomu Honma
IPC分类号： C08G63/06 , C08G63/02 , C08G63/688 , C08G63/78 , C12P7/62 , C12P11/00
CPC分类号： C12P11/00 , C08G63/6882 , C12P7/625
摘要： A polyhydroxyalkanoate that comprises a unit represented by the following chemical formula (1): wherein R1 is a substituent of an aromatic ring selected from the group consisting of H, CH3, C2H5, CH3CH2CH2, (CH3)2CH, (CH3)3C, a halogen atom, CN, NO2, COOR′, and SO2R″, wherein R′ is selected from the group consisting of H, Na, K, CH3, and C2H5, and R″ is selected from the group consisting of OH, a halogen atom, ONa, OK, OCH3, and OC2H5; and x represents an integer of 1 to 8 being the same or different each other in the polyhydroxyalkanoate. A method for producing the polyhydroxyalkanoate is also provided.
摘要翻译：一种聚羟基链烷酸酯，其包含由以下化学式（1）表示的单元：其中R1是选自H，CH 3，C 2， CH 3，CH 3，CH 2，CH 2，CH 3，CH 3，CH 3，CH 3，）CH 2（CH 3）3 C，卤素原子，CN，NO 2，COOR' ，和SO 2 R“，其中R'选自H，Na，K，CH 3，和C 2 H 5，并且R“选自OH，卤素原子，ONa，OK，OCH 3和OC 2， 5 H 5; x表示在聚羟基链烷酸酯中相同或不同的1〜8的整数。还提供了生产聚羟基链烷酸酯的方法。

37. 发明授权

US06808907B2 Method and apparatus for producing polyhydroxyalkanoate 失效
标题翻译：聚羟基链烷酸酯的制备方法和装置
公开(公告)号：US06808907B2
公开(公告)日：2004-10-26
申请号：US10105332
申请日：2002-03-26
申请人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Takashi Kenmoku
发明人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Takashi Kenmoku
IPC分类号： C12P762
CPC分类号： C12P7/625 , C12M47/06
摘要： The present invention provides a method for producing highly purified polyhydroxyalkanoate (PHA) with high yield, by removing cell components other than PHA from cells containing PHA. Moreover, the present invention provides a method for simply removing or reducing chlorine remained in the collected PHA particles, when a treatment with an oxidizing agent containing hypochlorite is performed in the production of PHA as described above. One of the above-described methods comprises a step of treating cells containing polyhydroxyalkanoate with an oxidizing agent containing at least hypochlorite, a step of separating the treated cells into a water-soluble fraction and a water-insoluble fraction, and a step of reducing chlorine remained in the water-insoluble fraction. The step of reducing chlorine may be a step of washing the water-insoluble fraction with a hot water, thiosulfate solution or polar solvent solution containing at least an organic polar solvent in which polyhydroxyalkanoate is insoluble.
摘要翻译：本发明提供了通过从含有PHA的细胞中除去PHA以外的细胞成分而以高产率生产高纯度聚羟基链烷酸酯（PHA）的方法。此外，本发明提供了如上所述在生产PHA中进行含有次氯酸盐的氧化剂的处理时，简单地除去或还原所收集的PHA颗粒中残留的氯的方法。上述方法之一包括用至少含有次氯酸盐的氧化剂处理含有聚羟基链烷酸酯的细胞的步骤，将经处理的细胞分离成水溶性级分和水不溶性级分的步骤以及还原氯保留在水不溶性部分中。还原氯的步骤可以是用至少含有聚羟基链烷酸酯不溶的有机极性溶剂的热水，硫代硫酸盐溶液或极性溶剂溶液洗涤水不溶性部分的步骤。

38. 发明授权

US06645743B1 Polyhydroxyalkanoate copolymer containing in molecule unit with vinylphenyl structure in its side chain and method of manufacturing the same 失效
标题翻译：新型聚羟基链烷酸酯共聚物在其侧链中分子单元含乙烯基苯基结构及其制造方法
公开(公告)号：US06645743B1
公开(公告)日：2003-11-11
申请号：US10372285
申请日：2003-02-25
申请人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Takashi Kenmoku , Tatsuki Fukui
发明人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Takashi Kenmoku , Tatsuki Fukui
IPC分类号： C12P742
CPC分类号： C08G63/06 , C08G63/6882 , C12P7/625 , C12P11/00 , Y10S435/874 , Y10S435/877
摘要： A polyhydroxyalkanoate (PHA) having a desired configuration is produced using a raw material containing &ohgr;-(4-vinylphenyl)-alkanoic acid and (&ohgr;-substituted alkanoic acid in which a group having a ring structure selected from phenyl, thienyl, and cyclohexyl structures substitutes therefor on the end thereof by producing a PHA copolymer containing the corresponding 3-hydroxy-&ohgr;-(4-vinylphenyl)-alkanoate unit and the corresponding 3-hydroxy-&ohgr;-substituted alkanoate unit by making use of a microorganism capable of producing the PHA or by oxidizing a predetermined portion of the corresponding PHA.
摘要翻译：使用含有ω-（4-乙烯基苯基） - 链烷酸的原料和（其中具有选自苯基，噻吩基和环己基结构的环结构的基团的（ω-取代的链烷酸）制备具有所需构型的聚羟基链烷酸酯（PHA）通过制备含有相应的3-羟基-ω-（4-乙烯基苯基） - 烷酸酯单元和相应的3-羟基-ω-取代的链烷酸酯单元的PHA共聚物，通过使用能够产生或通过氧化相应PHA的预定部分。

39. 发明申请

US20050054063A1 Method and apparatus for producing polyhydroxyalkanoate 审中-公开
标题翻译：聚羟基链烷酸酯的制备方法和装置
公开(公告)号：US20050054063A1
公开(公告)日：2005-03-10
申请号：US10940029
申请日：2004-09-14
申请人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Takashi Kenmoku
发明人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Takashi Kenmoku
IPC分类号： C08G63/90 , C12M1/00 , C12P7/62 , C12P7/26
CPC分类号： C12P7/625 , C12M47/06
摘要： The present invention provides a method for producing highly purified polyhydroxyalkanoate (PHA) with high yield, by removing cell components other than PHA from cells containing PHA. Moreover, the present invention provides a method for simply removing or reducing chlorine remained in the collected PHA particles, when a treatment with an oxidizing agent containing hypochlorite is performed in the production of PHA as described above. One of the above-described methods comprises a step of treating cells containing polyhydroxyalkanoate with an oxidizing agent containing at least hypochlorite, a step of separating the treated cells into a water-soluble fraction and a water-insoluble fraction, and a step of reducing chlorine remained in the water-insoluble fraction. The step of reducing chlorine may be a step of washing the water-insoluble fraction with a hot water, thiosulfate solution or polar solvent solution containing at least an organic polar solvent in which polyhydroxyalkanoate is insoluble.
摘要翻译：本发明提供了通过从含有PHA的细胞中除去PHA以外的细胞成分而以高产率生产高纯度聚羟基链烷酸酯（PHA）的方法。此外，本发明提供了如上所述在生产PHA中进行含有次氯酸盐的氧化剂的处理时，简单地除去或还原所收集的PHA颗粒中残留的氯的方法。上述方法之一包括用至少含有次氯酸盐的氧化剂处理含有聚羟基链烷酸酯的细胞的步骤，将经处理的细胞分离成水溶性级分和水不溶性级分的步骤以及还原氯保留在水不溶性部分中。还原氯的步骤可以是用至少含有聚羟基链烷酸酯不溶的有机极性溶剂的热水，硫代硫酸盐溶液或极性溶剂溶液洗涤水不溶性部分的步骤。

40. 发明授权

US06861496B2 Polyhydroxyalkonoate and method of producing same, and ω-(2-thienylsulfanyl) alkanoic acid and method of producing same 失效
标题翻译：聚羟基链烷酸酯及其制造方法，ω-（2-噻吩基硫烷基）链烷酸及其制造方法
公开(公告)号：US06861496B2
公开(公告)日：2005-03-01
申请号：US10641090
申请日：2003-08-15
申请人： Takashi Kenmoku , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Tsutomu Honma
发明人： Takashi Kenmoku , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Tsutomu Honma
IPC分类号： C08G63/60 , C07D333/34 , C08G63/68 , C08G63/688 , C12P7/62 , C12P11/00 , C12R1/38 , C08G63/02
CPC分类号： C12P7/625 , C07D333/34 , C08G63/6882 , C12P11/00 , Y10S435/874 , Y10S435/877
摘要： PHA containing a novel 3-hydroxy-thioalkanoic acid unit having a highly reactive thienyl group in a side chain thereof, and a method of producing the same are provided. Specifically, 5-(2-thienylsulfanyl) valeric acid represented by Chemical Formula [4] below and 6-(2-thienylsulfanyl) hexanoic acid represented by Chemical Formula [5] below are provided. Further, a method of producing PHA, comprising the step of collecting PHA from cells of a microorganism cultured in a medium containing the valeric acid or hexanoic acid, and a novel PHA represented by Chemical Formula [1] below are provided. (n denotes an integer of 1 to 9)
摘要翻译：提供了含有其侧链中具有高反应性噻吩基的新型3-羟基 - 硫代链烷酸单元的PHA及其制备方法。具体地，提供由下述化学式[4]表示的5-（2-噻吩基硫烷基）戊酸和由下述化学式[5]表示的6-（2-噻吩基硫烷基）己酸。此外，提供了一种制备PHA的方法，其包括从含有戊酸或己酸的培养基中培养的微生物的细胞和由下述化学式[1]表示的新型PHA收集PHA的步骤（n表示 1〜9的整数）

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