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    • 37. 发明授权
    • Method and apparatus for producing polyhydroxyalkanoate
    • 聚羟基链烷酸酯的制备方法和装置
    • US06808907B2
    • 2004-10-26
    • US10105332
    • 2002-03-26
    • Tsutomu HonmaEtsuko SugawaTetsuya YanoTsuyoshi NomotoTakeshi ImamuraTomohiro SuzukiTakashi Kenmoku
    • Tsutomu HonmaEtsuko SugawaTetsuya YanoTsuyoshi NomotoTakeshi ImamuraTomohiro SuzukiTakashi Kenmoku
    • C12P762
    • C12P7/625C12M47/06
    • The present invention provides a method for producing highly purified polyhydroxyalkanoate (PHA) with high yield, by removing cell components other than PHA from cells containing PHA. Moreover, the present invention provides a method for simply removing or reducing chlorine remained in the collected PHA particles, when a treatment with an oxidizing agent containing hypochlorite is performed in the production of PHA as described above. One of the above-described methods comprises a step of treating cells containing polyhydroxyalkanoate with an oxidizing agent containing at least hypochlorite, a step of separating the treated cells into a water-soluble fraction and a water-insoluble fraction, and a step of reducing chlorine remained in the water-insoluble fraction. The step of reducing chlorine may be a step of washing the water-insoluble fraction with a hot water, thiosulfate solution or polar solvent solution containing at least an organic polar solvent in which polyhydroxyalkanoate is insoluble.
    • 本发明提供了通过从含有PHA的细胞中除去PHA以外的细胞成分而以高产率生产高纯度聚羟基链烷酸酯(PHA)的方法。 此外,本发明提供了如上所述在生产PHA中进行含有次氯酸盐的氧化剂的处理时,简单地除去或还原所收集的PHA颗粒中残留的氯的方法。 上述方法之一包括用至少含有次氯酸盐的氧化剂处理含有聚羟基链烷酸酯的细胞的步骤,将经处理的细胞分离成水溶性级分和水不溶性级分的步骤以及还原氯 保留在水不溶性部分中。 还原氯的步骤可以是用至少含有聚羟基链烷酸酯不溶的有机极性溶剂的热水,硫代硫酸盐溶液或极性溶剂溶液洗涤水不溶性部分的步骤。
    • 39. 发明申请
    • Method and apparatus for producing polyhydroxyalkanoate
    • 聚羟基链烷酸酯的制备方法和装置
    • US20050054063A1
    • 2005-03-10
    • US10940029
    • 2004-09-14
    • Tsutomu HonmaEtsuko SugawaTetsuya YanoTsuyoshi NomotoTakeshi ImamuraTomohiro SuzukiTakashi Kenmoku
    • Tsutomu HonmaEtsuko SugawaTetsuya YanoTsuyoshi NomotoTakeshi ImamuraTomohiro SuzukiTakashi Kenmoku
    • C08G63/90C12M1/00C12P7/62C12P7/26
    • C12P7/625C12M47/06
    • The present invention provides a method for producing highly purified polyhydroxyalkanoate (PHA) with high yield, by removing cell components other than PHA from cells containing PHA. Moreover, the present invention provides a method for simply removing or reducing chlorine remained in the collected PHA particles, when a treatment with an oxidizing agent containing hypochlorite is performed in the production of PHA as described above. One of the above-described methods comprises a step of treating cells containing polyhydroxyalkanoate with an oxidizing agent containing at least hypochlorite, a step of separating the treated cells into a water-soluble fraction and a water-insoluble fraction, and a step of reducing chlorine remained in the water-insoluble fraction. The step of reducing chlorine may be a step of washing the water-insoluble fraction with a hot water, thiosulfate solution or polar solvent solution containing at least an organic polar solvent in which polyhydroxyalkanoate is insoluble.
    • 本发明提供了通过从含有PHA的细胞中除去PHA以外的细胞成分而以高产率生产高纯度聚羟基链烷酸酯(PHA)的方法。 此外,本发明提供了如上所述在生产PHA中进行含有次氯酸盐的氧化剂的处理时,简单地除去或还原所收集的PHA颗粒中残留的氯的方法。 上述方法之一包括用至少含有次氯酸盐的氧化剂处理含有聚羟基链烷酸酯的细胞的步骤,将经处理的细胞分离成水溶性级分和水不溶性级分的步骤以及还原氯 保留在水不溶性部分中。 还原氯的步骤可以是用至少含有聚羟基链烷酸酯不溶的有机极性溶剂的热水,硫代硫酸盐溶液或极性溶剂溶液洗涤水不溶性部分的步骤。