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31. 发明授权

US5936434A Voltage comparator and A/D converter 失效
标题翻译：电压比较器和A / D转换器
公开(公告)号：US5936434A
公开(公告)日：1999-08-10
申请号：US912813
申请日：1997-08-19
申请人： Toshio Kumamoto , Masao Ito , Takahiro Miki , Takashi Okuda
发明人： Toshio Kumamoto , Masao Ito , Takahiro Miki , Takashi Okuda
IPC分类号： H03M1/34 , H03F3/45 , H03K5/08 , H03K5/24 , H03K5/153
CPC分类号： H03K5/249 , H03K5/2481
摘要： An object is to obtain a voltage comparator capable of high-accuracy voltage comparison. An input voltage (VIN) and a reference voltage (VREF) are connected to one electrode of a capacitor (C1) through switches (S1) and (S2), respectively. The other electrode of the capacitor (C1) is connected to the input portion of an inverter (INV1). The output portion of the inverter (INV1) is connected to the input portion of an inverter (INV3) and is also fed back to the input through a switch (S3). An inverter (INV11) is further connected in parallel with the inverter (INV1), wherein the input/output characteristics of the inverters (INV1, INV3 and INV11) are set equal.
摘要翻译：目的是获得能够进行高精度电压比较的电压比较器。分别通过开关（S1）和（S2）将输入电压（VIN）和参考电压（VREF）连接到电容器（C1）的一个电极。电容器（C1）的另一个电极连接到逆变器（INV1）的输入部分。逆变器（INV1）的输出部分连接到逆变器（INV3）的输入部分，并通过开关（S3）反馈到输入端。反相器（INV11）进一步与反相器（INV1）并联连接，其中反相器（INV1，INV3和INV11）的输入/输出特性被设定为相等。

32. 发明申请

US20130069132A1 SEMICONDUCTOR STORAGE DEVICE 有权
标题翻译：半导体存储设备
公开(公告)号：US20130069132A1
公开(公告)日：2013-03-21
申请号：US13606472
申请日：2012-09-07
申请人： Tomoaki Atsumi , Takashi Okuda
发明人： Tomoaki Atsumi , Takashi Okuda
IPC分类号： H01L27/108 , H01L23/485
CPC分类号： H01L27/112 , H01L21/76801 , H01L21/76826 , H01L27/0688 , H01L27/10805 , H01L27/1085 , H01L27/10873 , H01L27/10897 , H01L27/1156 , H01L27/1207 , H01L27/1225 , H01L29/16 , H01L29/78 , H01L29/7869
摘要： Probability of malfunction of a semiconductor storage device is reduced. A shielding layer is provided between a memory cell array (e.g., a memory cell array including a transistor formed using an oxide semiconductor material) and a peripheral circuit (e.g., a peripheral circuit including a transistor formed using a semiconductor substrate), which are stacked. With this structure, the memory cell array and the peripheral circuit can be shielded from radiation noise generated between the memory cell array and the peripheral circuit. Thus, probability of malfunction of the semiconductor storage device can be reduced.
摘要翻译：减少了半导体存储装置故障的概率。屏蔽层设置在堆叠的存储单元阵列（例如，包括使用氧化物半导体材料形成的晶体管的存储单元阵列）和周边电路（例如，包括使用半导体衬底形成的晶体管的外围电路）之间。利用这种结构，存储单元阵列和外围电路可以屏蔽在存储单元阵列和外围电路之间产生的辐射噪声。因此，可以降低半导体存储装置的故障概率。

33. 发明申请

US20110281349A1 Insect Desiccation Resistance Genes and Uses Thereof 有权
标题翻译：昆虫抗病基因及其用途
公开(公告)号：US20110281349A1
公开(公告)日：2011-11-17
申请号：US13103806
申请日：2011-05-09
申请人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Kazuei Mita , Keiko Kadono
发明人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Kazuei Mita , Keiko Kadono
IPC分类号： C12N5/10 , C12N15/63 , C07H21/04
CPC分类号： C07K14/43563
摘要： An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins (PvLEA1, PvLEA2, and PvLEA3.) When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from PvLEA1, 2 and 3 genes were heat soluble even when boiling, so that PvLEA1, 2 and 3 proteins have highly hydrophilic property as well as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes. Furthermore, introduction of these PvLEA 1, 2 and 3 genes into animal cells successfully conferred desiccation resistance to the cells. The present invention provides the first example of LEA genes isolated from insects.
摘要翻译：本发明的目的在于提供编码昆虫抗干扰性蛋白质的多核苷酸及其使用方法，从干燥状态的Polypedilum vanderplanki幼虫生产cDNA文库，构建P.vanderplanki EST数据库，分离编码LEA蛋白质的基因。这导致了三种类型的编码LEA样蛋白的新基因（PvLEA1，PvLEA2和PvLEA3）的成功分离。当对从每个基因推导的蛋白进行二级结构预测和基序搜索时，所有三种蛋白都具有α 富含螺旋的结构和LEA_4基序，是LEA蛋白的特征。此外，由PvLEA1,2和3基因合成的重组蛋白即使在沸腾时也是热溶性的，因此PvLEA1,2和3蛋白具有高亲水性以及植物LEA蛋白。因此，发现三个分离的基因是新的P. vanderplanki衍生的LEA基因。此外，将这些PvLEA 1,2和3基因引入动物细胞成功地赋予细胞干燥抗性。本发明提供了从昆虫分离的LEA基因的第一个实例。

34. 发明申请

US20090111176A1 Trehalose transporter gene and method of introducing trehalose into cells 有权
标题翻译：海藻糖转运蛋白基因和将海藻糖引入细胞的方法
公开(公告)号：US20090111176A1
公开(公告)日：2009-04-30
申请号：US12073457
申请日：2008-03-05
申请人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Ayako Saito , Yasushi Kanamori , Yuichi Nakahara
发明人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Ayako Saito , Yasushi Kanamori , Yuichi Nakahara
IPC分类号： C12N5/06 , C12N15/11 , C07K14/00 , C12N15/00
CPC分类号： C07K14/43563 , C07K14/43577
摘要： There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched in P. vanderplanki EST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 in Xenopus oocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.
摘要翻译：提供海藻糖转运蛋白基因和通过使用该基因将海藻糖引入细胞的方法。在P. vanderplanki EST中搜索海藻糖转运蛋白基因的候选物，得到获得的命名为Tret1的cDNA。 Tret1编码具有12个跨膜结构的504个氨基酸的蛋白质。 Tret1表达由干燥应激诱导，在脂肪体中占优势。非洲爪蟾卵母细胞中TRET1的功能表达显示，转运活性对海藻糖是特异性的，独立于细胞外pH和电化学膜电位。根据海藻糖的浓度梯度，TRET1的转运方向是可逆的。 TRET1对于海藻糖的表观Km和Vmax是非常高的值。这些结果表明TRET1是一种促进的高容量海藻糖特异性转运蛋白。 Tret1在昆虫中广泛存在。此外，TRET1在包括脊椎动物以及昆虫的细胞中赋予海藻糖通透性。

35. 发明授权

US06936707B2 Modified DNA molecule, recombinant containing the same, and uses thereof 有权
标题翻译：修饰的DNA分子，含有其的重组体及其用途
公开(公告)号：US06936707B2
公开(公告)日：2005-08-30
申请号：US09901572
申请日：2001-07-11
申请人： Takashi Okuda , Shuji Saito , Kristi M. Moore , Yoshinari Tsuzaki
发明人： Takashi Okuda , Shuji Saito , Kristi M. Moore , Yoshinari Tsuzaki
IPC分类号： C07K14/055 , C07K14/30 , C12N1/21 , C12N7/01 , C12N15/863 , C07H21/00 , A61K39/02 , C12N15/11 , C12N15/31 , C12N15/79
CPC分类号： C12N15/86 , C07K14/005 , C07K14/30 , C07K2319/00 , C07K2319/02 , C12N2710/16322 , C12N2710/24143
摘要： There is provided a DNA molecule derived from a prokaryotic cell in which at least one of the DNA regions encoding NXB (N is asparagine, X is any amino acid other than proline, and B is serine or threonine) has been modified so that no N-glycosylation occurs during the expression in a eukaryotic cell, and since the DNA molecule has been modified at the N-glycosylation site, it produces a non-N-glycosylated protein, which thereby exhibits a high immunogenicity when, for example, it is allowed to produce, in a eukaryotic cell, an antigen protein derived from a prokaryotic cell.
摘要翻译：提供了衍生自原核细胞的DNA分子，其中编码NXB（N为天冬酰胺，X为除脯氨酸以外的任何氨基酸，B为丝氨酸或苏氨酸）的DNA区域中的至少一个已被修饰，使得不存在N 在真核细胞中表达期间发生糖基化，并且由于DNA分子在N-糖基化位点被修饰，所以它产生非N-糖基化的蛋白质，从而当例如允许的时候表现出高的免疫原性在真核细胞中产生源自原核细胞的抗原蛋白。

36. 发明授权

US09318374B2 Semiconductor storage device comprising peripheral circuit, Shielding layer, and memory cell array 有权
标题翻译：半导体存储装置包括外围电路，屏蔽层和存储单元阵列
公开(公告)号：US09318374B2
公开(公告)日：2016-04-19
申请号：US13606472
申请日：2012-09-07
申请人： Tomoaki Atsumi , Takashi Okuda
发明人： Tomoaki Atsumi , Takashi Okuda
IPC分类号： H01L21/768 , H01L27/06 , H01L27/108 , H01L27/12 , H01L27/115
CPC分类号： H01L27/112 , H01L21/76801 , H01L21/76826 , H01L27/0688 , H01L27/10805 , H01L27/1085 , H01L27/10873 , H01L27/10897 , H01L27/1156 , H01L27/1207 , H01L27/1225 , H01L29/16 , H01L29/78 , H01L29/7869
摘要： Probability of malfunction of a semiconductor storage device is reduced. A shielding layer is provided between a memory cell array (e.g., a memory cell array including a transistor formed using an oxide semiconductor material) and a peripheral circuit (e.g., a peripheral circuit including a transistor formed using a semiconductor substrate), which are stacked. With this structure, the memory cell array and the peripheral circuit can be shielded from radiation noise generated between the memory cell array and the peripheral circuit. Thus, probability of malfunction of the semiconductor storage device can be reduced.
摘要翻译：减少了半导体存储装置故障的概率。屏蔽层设置在堆叠的存储单元阵列（例如，包括使用氧化物半导体材料形成的晶体管的存储单元阵列）和周边电路（例如，包括使用半导体衬底形成的晶体管的外围电路）之间。利用这种结构，存储单元阵列和外围电路可以屏蔽在存储单元阵列和外围电路之间产生的辐射噪声。因此，可以降低半导体存储装置的故障概率。

37. 发明授权

US07892789B2 Method of increasing cell permeability to trehalose by recombinantly producing a trehalose transporter 有权
标题翻译：通过重组产生海藻糖转运蛋白增加海藻糖的细胞渗透性的方法
公开(公告)号：US07892789B2
公开(公告)日：2011-02-22
申请号：US12073457
申请日：2008-03-05
申请人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Ayako Saito , Yasushi Kanamori , Yuichi Nakahara
发明人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Ayako Saito , Yasushi Kanamori , Yuichi Nakahara
IPC分类号： C12P21/06
CPC分类号： C07K14/43563 , C07K14/43577
摘要： There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched in P. vanderplanki EST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 in Xenopus oocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.
摘要翻译：提供海藻糖转运蛋白基因和通过使用该基因将海藻糖引入细胞的方法。在P. vanderplanki EST中搜索海藻糖转运蛋白基因的候选物，得到获得的命名为Tret1的cDNA。 Tret1编码具有12个跨膜结构的504个氨基酸的蛋白质。 Tret1表达由干燥应激诱导，在脂肪体中占优势。非洲爪蟾卵母细胞中TRET1的功能表达显示，转运活性对海藻糖是特异性的，独立于细胞外pH和电化学膜电位。根据海藻糖的浓度梯度，TRET1的转运方向是可逆的。 TRET1对于海藻糖的表观Km和Vmax是非常高的值。这些结果表明TRET1是一种促进的高容量海藻糖特异性转运蛋白。 Tret1在昆虫中广泛存在。此外，TRET1在包括脊椎动物以及昆虫的细胞中赋予海藻糖通透性。

38. 发明授权

US07473769B2 Insect desiccation resistance genes and uses thereof 失效
标题翻译：昆虫干燥抗性基因及其用途
公开(公告)号：US07473769B2
公开(公告)日：2009-01-06
申请号：US11222641
申请日：2005-09-08
申请人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Kazuei Mita , Keiko Kadono
发明人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Kazuei Mita , Keiko Kadono
IPC分类号： C07H21/04 , A61K38/00
CPC分类号： C07K14/43563
摘要： An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof. cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins. When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from these genes were heat soluble even when boiling, so that these proteins have hydrophilic property as high as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes. Furthermore, introduction of these genes into animal cells successfully conferred desiccation resistance to the cells.
摘要翻译：本发明的目的是提供编码昆虫耐干燥性蛋白质的多核苷酸及其用途。在干燥状态下从Polypedilum vanderplanki幼虫生产cDNA文库，构建了P.vanderplanki EST数据库，分离了编码LEA蛋白的基因。这导致了三种类型的编码LEA样蛋白的新基因的成功分离。对从每个基因推导的蛋白质进行二级结构预测和基序搜索时，所有三种蛋白质都具有富含α-螺旋的结构和LEA_4基序，这是LEA蛋白质的特征。此外，由这些基因合成的重组蛋白即使在沸腾时也是热溶性的，因此这些蛋白质具有与植物LEA蛋白一样高的亲水性。因此，发现三个分离的基因是新的P. vanderplanki衍生的LEA基因。此外，将这些基因导入动物细胞成功地赋予细胞干燥抗性。

39. 发明授权

US06962774B2 Method for dry-preserving multicellular organism tissue at ordinary temperatures 失效
标题翻译：常温保存多细胞生物体组织的方法
公开(公告)号：US06962774B2
公开(公告)日：2005-11-08
申请号：US10659439
申请日：2003-09-11
申请人： Takashi Okuda , Masahiko Watanabe , Takahiro Kikawada
发明人： Takashi Okuda , Masahiko Watanabe , Takahiro Kikawada
IPC分类号： A01N1/02 , A23K1/18 , C12N1/04 , C12N5/00 , C12N5/06
CPC分类号： C12N1/04 , A01N1/02 , A01N1/0278 , A23K50/80
摘要： A tissue of a multicellular organism is gradually dried during cultivation. After the tissue has been completely dehydrated, water is added to the tissue for its recovery. The tissue of the multicellular organism is submerged in an insect body fluid medium treated with heat, and dried for 48 hours or more.
摘要翻译：多细胞生物体的组织在培养期间逐渐干燥。在组织完全脱水后，将水加入到组织中进行回收。将多细胞生物体的组织浸没在用热处理的昆虫体液培养基中，干燥48小时以上。

40. 发明申请

US20050163132A1 L2 switch 有权
标题翻译： L2开关
公开(公告)号：US20050163132A1
公开(公告)日：2005-07-28
申请号：US11077303
申请日：2005-03-10
申请人： Hironori Mieno , Youichi Konuma , Jyunichi Shimada , Koichi Saiki , Takashi Okuda , Takanori Sasaki
发明人： Hironori Mieno , Youichi Konuma , Jyunichi Shimada , Koichi Saiki , Takashi Okuda , Takanori Sasaki
IPC分类号： H04L12/44 , H04L12/46 , H04L12/56
CPC分类号： H04L12/465
摘要： In an L2 switch having a VLAN function for configuring a layer 2 network in a star topology, a mesh topology, a ring topology, or the like, a VLAN functional portion maps a frame to a predetermined VLAN, a frame classifying portion assigns to the frame a first tag indicating a priority, and a policing portion assigns to the frame a second tag indicating whether or not the frame can be discarded. Also, an OAM monitor inserts a monitoring frame to the VLAN.
摘要翻译：在具有VLAN功能的L2交换机中，用于配置星型拓扑中的二层网络，网状拓扑，环形拓扑等，VLAN功能部分将帧映射到预定VLAN，帧分类部分分配给构成指示优先级的第一标签，并且监管部分向该帧分配指示该帧是否可以被丢弃的第二标签。另外，OAM监视器将监控帧插入VLAN。

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