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31. 发明申请

US20140011707A1 BIOLOGICAL CHIP HYBRIDIZATION SYSTEM 有权
标题翻译：生物芯片混合系统
公开(公告)号：US20140011707A1
公开(公告)日：2014-01-09
申请号：US14006540
申请日：2012-02-10
申请人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
发明人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , B01L3/5027 , B01L7/52 , B01L2300/0636 , B01L2300/0816 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/0622 , G01N33/543 , G01N2035/00158
摘要： A hybridization system is provided, which comprises a biological chip having a substrate (1) with a probe dot matrix (3) and a cover plate (2) with at least two through-holes (4), where a hybrid chamber (5) is formed between the substrate (1) and the cover plate (2), at least two fluid channels (6) are interconnected respectively with the hybrid chamber (5) by the two through-holes (4), and a fluid control device is interconnected with the fluid channels (6). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels (6) and the chamber (5) to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.
摘要翻译：提供了一种杂交系统，其包括具有带有探针点阵（3）的基底（1）和具有至少两个通孔（4）的盖板（2）的生物芯片，其中混合室（5）形成在基板（1）和盖板（2）之间，至少两个流体通道（6）分别通过两个通孔（4）与混合室（5）相互连接，并且流体控制装置与流体通道（6）互连。生物芯片杂交系统集成了杂交，清洁和干燥功能，使用由流体控制装置提供的电力作为促进液体流动的驱动力，用于在流体通道（6）和腔室（5）中自动往复流动，以实现动态杂交，从而提高混合效率和均匀性，实现自动控制。

32. 发明申请

US20140010733A1 INTERFACE DEVICE FOR BIO-CHIP 有权
标题翻译：用于生物芯片的界面设备
公开(公告)号：US20140010733A1
公开(公告)日：2014-01-09
申请号：US14006512
申请日：2012-02-10
申请人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Wanli Xing
发明人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Wanli Xing
IPC分类号： B01L3/00
CPC分类号： B01L3/502 , B01L3/502715 , B01L3/502784 , B01L2200/0689 , B01L2200/141 , B01L2300/0816 , B01L2300/0874 , B01L2300/0887 , B01L2400/0487
摘要： An interface device for bio-chip at least comprises an interface unit, said interface unit consisting of instrument interface layer, fluid channel layer and sample interface layer. The instrument interface layer has at least one instrument interface. The fluid channel layer has one hollow-out fluid channel. The sample interface layer has at least one sample interface, and both ends of said fluid channel connect to the sample interface and the instrument interface respectively. The interface device separates sample solutions from the instrument interface by gas or liquid in the fluid channel, thus to avoid direct contacts between sample solutions and instrument interface which will cause pollution, and omitting cleaning processes after using instruments. With simple structure, easy operation and low cost, it applies to chemistry field, biology field and medical analysis field.
摘要翻译：用于生物芯片的接口装置至少包括接口单元，所述接口单元由仪器接口层，流体通道层和样品界面层组成。仪器界面层至少有一个仪器界面。流体通道层具有一个中空的流体通道。样品接口层具有至少一个样品接口，并且所述流体通道的两端分别连接到样品接口和仪器接口。界面设备通过液体通道中的气体或液体将样品溶液与仪器界面分离，从而避免样品溶液与仪器界面之间的直接接触，从而造成污染，并在使用仪器后省略清洗过程。结构简单，操作方便，成本低，适用于化学领域，生物学领域和医学分析领域。

33. 发明申请

US20140011703A1 AUTOMATIC INJECTION DEVICE FOR MICROARRAY CHIP AND AUTOMATIC INJECTION HYBRIDIZATION MICROARRAY CHIP 有权
标题翻译：微胶囊自动注射装置和自动注射微胶囊芯片
公开(公告)号：US20140011703A1
公开(公告)日：2014-01-09
申请号：US14006488
申请日：2012-02-10
申请人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Wanli Xing
发明人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Wanli Xing
IPC分类号： G01N1/10
CPC分类号： G01N1/10 , C12Q1/6813 , C40B30/04 , C40B40/06 , C40B40/10 , C40B40/12 , G01N33/543 , G01N33/68 , G01N35/1095
摘要： An automatic injection device comprises at least an injection unit (1). The said injection unit (1) is formed by sealing a cover plate layer (3) with hydrophilic surfaces and a microfluid layer (4). The said cover plate layer (3) is provided with at least two through holes (5). he said microfluid layer (4) is provided with a hollow-out hybridization chamber (7) and at least two hollow-out microfluid channels (6). One end of each channel (6) is connected with the hybridization chamber (7), and the other end is connected with a through hole (5) of the cover plate layer (3) respectively. Taking advantage of the hydrophilicity of the cover plate, the automatic injection device makes a solution automatically enter and fill the hybridization chamber (7) and the microfluid channels (6) by the driving force of liquid surface tension. The flow uniformity of sample solution in microarray chip is achieved by the structural design of the hybridization chamber (7) and the microfluid channels (6). The automatic injection device has advantages of simple manufacture, easy operation, high hybridization efficiency, low sample cost, and automatic quantificational injection.
摘要翻译：自动注射装置至少包括注射单元（1）。所述注射单元（1）通过密封具有亲水表面的盖板层（3）和微流体层（4）而形成。所述盖板层（3）设置有至少两个通孔（5）。他表示微流体层（4）设置有中空杂交室（7）和至少两个中空的微流体通道（6）。每个通道（6）的一端与杂交室（7）连接，另一端分别与盖板层（3）的通孔（5）连接。利用盖板的亲水性，自动注射装置通过液面张力的驱动力使溶液自动进入并填充杂交室（7）和微流体通道（6）。微阵列芯片中样品溶液的流动均匀性通过杂交室（7）和微流体通道（6）的结构设计来实现。自动注射装置制造简单，操作简便，杂交效率高，样品成本低，自动定量注射等优点。

34. 发明申请

US20140004557A1 MICROFLUIDIC DEVICE AND ITS USE FOR POSITIONING OF CELLS OR ORGANISMS 有权
标题翻译：微流体装置及其用于定位细胞或有机物的用途
公开(公告)号：US20140004557A1
公开(公告)日：2014-01-02
申请号：US13991848
申请日：2012-01-13
申请人： Rui Ma , Lan Xie , Tian Qiu , Lei Wang , Wanli Xing
发明人： Rui Ma , Lan Xie , Tian Qiu , Lei Wang , Wanli Xing
IPC分类号： B01L3/00
CPC分类号： B01L3/5027 , C12M21/06 , C12M23/12 , C12M23/16
摘要： A micro fluidic device comprises one microstructure layer (5) and one cover layer (1), wherein the cover layer (1) is connected to the microstructure layer (5). The microstructure layer (5) comprises one bottom layer and a plurality of microstructures on it to position samples. The cover layer (1) comprises one top layer, one positioning well (6) and at least one inlet pool (4). The positioning well (6) is right above the microstructures and connected with each other. The inlet pools (4) and the positioning well (6) are connected by microchannels (3) which are formed between the microstructure layer (5) and the cover layer (1). The micro fluidic device can be applied in vitro fertilization, in determining how glial cells affect neurons, in constructing neural network and in detecting cell growth conditions.
摘要翻译：微流体装置包括一个微结构层（5）和一个覆盖层（1），其中覆盖层（1）连接到微结构层（5）。微结构层（5）包括一个底层和多个微结构，用于定位样品。覆盖层（1）包括一个顶层，一个定位孔（6）和至少一个入口池（4）。定位井（6）位于微结构的正上方并相互连接。入口池（4）和定位井（6）通过形成在微结构层（5）和覆盖层（1）之间的微通道（3）连接。微流体装置可以应用于体外受精，确定神经胶质细胞如何影响神经元，构建神经网络和检测细胞生长条件。

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