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    • 34. 发明授权
    • Screening of agents for treatment of alzheimer's disease
    • 筛查治疗阿尔茨海默病的药物
    • US06245575B1
    • 2001-06-12
    • US09194127
    • 1999-07-16
    • Michel GoedertRoss JakesMaria Grazia SpillantiniMasato HasegawaMichael John SmithRichard Anthony Crowther
    • Michel GoedertRoss JakesMaria Grazia SpillantiniMasato HasegawaMichael John SmithRichard Anthony Crowther
    • G01N3300
    • G01N33/6896Y10T436/144444
    • A method of screening an agent for potential use in the treatment of Alzheimer's disease, comprises reacting, in the presence of the agent, tau protein with a suitable sulphated carbohydrate under appropriate conditions to form filaments, and monitoring for the presence of filaments. Tau protein and sulphated carbohydrate, e.g. sulphated glycosaminoglycan, will react under appropriate conditions to form filaments, either paired helical filaments or straight filaments. If filament formation is affected when the reaction is carried out in the presence of an agent being screened, this is possibly due to an interfering, inhibiting or blocking effect of the agent. An agent which inhibits assembly of PHFs in vitro may also have an inhibiting effect in vivo and thus have potential therapeutic value in delaying the dementing effects of Alzheimer's disease. The invention can thus provide a screen to identify agents worthy of further investigation for use in the treatment of Alzheimer's disease.
    • 筛选用于治疗阿尔茨海默病的潜在用途的试剂的方法包括在适当条件下,在试剂存在下,将tau蛋白与合适的硫酸化碳水化合物反应以形成细丝并监测长丝的存在。 Tau蛋白和硫酸化碳水化合物,例如 硫酸化糖胺聚糖,将在合适的条件下反应形成细丝,成对的螺旋丝或直丝。 如果在被筛选的试剂存在下进行反应时影响细丝形成,这可能是由于试剂的干扰,抑制或阻断作用。 体外抑制PHF装配的药剂也可能在体内具有抑制作用,因此具有延缓阿尔茨海默氏病的痴呆作用的潜在治疗价值。 因此,本发明可以提供一种筛选器,用于鉴定用于治疗阿尔茨海默病的值得进一步研究的药剂。
    • 40. 发明申请
    • BIOLOGICAL SAMPLE STORAGE DEVICE
    • 生物样本储存装置
    • US20130260477A1
    • 2013-10-03
    • US13990144
    • 2011-11-24
    • Michael John SmithStevan Paul TortorellaGerard B. O'Beirne
    • Michael John SmithStevan Paul TortorellaGerard B. O'Beirne
    • B01L3/00
    • B01L3/508B01L9/52B01L2200/025B01L2200/12B01L2300/02B01L2300/069B01L2300/0819B01L2300/0822G01N1/312G01N35/00732Y10T436/25
    • Embodiments of the invention relate to methods of assembling an apparatus for holding biological samples. Conventionally, storage media are encased in a relatively thin flexible cardboard frame. Such constructions are vulnerable to damage from moisture and mechanical forces; these defects may subsequently cause problems for automated handling systems. In an embodiment, these defects are prevented by providing a pair of rigid frames, each frame comprising an aperture; placing a biological sample storage medium between the two frames so that the biological sample storage medium at least partly overlaps each of the apertures in the frames; and joining the two frames to fix the biological sample storage medium in place while allowing access to the biological sample storage medium via each of the apertures. Thus, in embodiments of the present invention, the frames can be rigid and waterproof, and provide a robust storage media for biological samples. At least one pre-defined area of weakness can be provided in the frames, to allow removal of a portion of the frames.
    • 本发明的实施例涉及组装用于保持生物样品的装置的方法。 通常,存储介质被封装在相对薄的柔性纸板框架中。 这种结构容易受到水分和机械力的损害; 这些缺陷可能随后引起自动化处理系统的问题。 在一个实施例中,通过提供一对刚性框架来防止这些缺陷,每个框架包括孔; 将生物样品存储介质放置在两个框架之间,使得生物样品存储介质至少部分地与框架中的每个孔重叠; 并且连接两个框架以将生物样品存储介质固定到位,同时允许经由每个孔进入生物样品存储介质。 因此,在本发明的实施例中,框架可以是刚性和防水的,并为生物样品提供坚固的存储介质。 可以在框架中提供至少一个预定义的弱点区域,以允许移除框架的一部分。