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    • 34. 发明申请
    • Method to assay test substances
    • 测定物质的方法
    • US20070111262A1
    • 2007-05-17
    • US11272050
    • 2005-11-14
    • Min LiHaiyan SunSojin Shikano
    • Min LiHaiyan SunSojin Shikano
    • G01N33/567G01N33/53
    • G01N33/566G01N33/5008G01N2500/00
    • Membrane proteins represent ˜30% of the proteome of both prokaryotes and eukaryotes. Unique to cell surface receptors is their biogenesis pathway, which involves vesicular trafficking from the endoplasmic reticulum through the Golgi apparatus and to the cell surface. Increasing evidence suggests specific regulation of biogenesis for different membrane receptors, hence affecting their surface expression. A pulse-chase assay can be used to monitor function recovery after chemobleaching (FRAC) to probe the transit time of the cell surface receptors to reach the cell surface. This method distinguishes molecular density from functional density. The ability of the reported method to access the biogenesis pathways in a high-throughput manner facilitates the identification and evaluation of molecules affecting receptor trafficking.
    • 膜蛋白占原核生物和真核生物蛋白质组的约30%。 细胞表面受体独特的是其生物发生途径,其涉及从内质网通过高尔基体和细胞表面的水泡运输。 越来越多的证据表明对不同膜受体的生物发生的特异性调节,因此影响其表面表达。 脉冲追踪测定可用于监测化学漂白(FRAC)后的功能恢复,以探测细胞表面受体到达细胞表面的转运时间。 该方法区分分子密度与功能密度。 报道的方法以高通量方式进入生物发生途径的能力有助于鉴定和评估影响受体运输的分子。