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31. 发明授权

US06861246B2 L-lysine-producing corynebacteria and process for the preparation of lysine 有权
标题翻译：产L-赖氨酸棒状杆菌和制备赖氨酸的方法
公开(公告)号：US06861246B2
公开(公告)日：2005-03-01
申请号：US09810521
申请日：2001-03-19
申请人： Caroline Kreutzer , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C07K14/34 , C12N15/67 , C12N15/77 , C12P13/08 , C12N1/20 , C12N9/00 , C12N9/12 , C12N9/88
CPC分类号： C07K14/34 , C12N15/67 , C12N15/77
摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译：本发明涉及具有增强的pyc基因（丙酮酸羧化酶基因）的棒状杆菌L-赖氨酸生产菌株，其中选自dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因），lysE基因（赖氨酸输出载体基因）和dapB基因（二氢吡啶二羧酸还原酶基因），特别是dapA基因，特别是过度表达，以及制备L-赖氨酸的方法。

32. 发明授权

US06200785B1 L-lysine-producing corynebacteria and process for the preparation of l-lysine 有权
标题翻译：产L-赖氨酸棒状细菌及其制备方法
公开(公告)号：US06200785B1
公开(公告)日：2001-03-13
申请号：US09353133
申请日：1999-07-14
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfeffere , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfeffere , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C12P1308
CPC分类号： C07K14/34 , C12N15/52 , C12P13/08
摘要： The invention relates to L-lysine-producing strains of corynebacteria with amplified lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are amplified and, in particular, overexpressed, and to a process for the preparation of L-lysine.
摘要翻译：本发明涉及具有扩增的lysE基因（赖氨酸出口载体基因）的棒状杆菌的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因），dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被扩增，特别是过表达，以及制备L-赖氨酸的方法。

33. 发明申请

US20100261233A1 L-LYSINE-PRODUCING CORYNEBACTERIA AND PROCESS FOR THE PREPARATION OF L-LYSINE 有权
标题翻译：生产L-赖氨酸的L-赖氨酸和L-赖氨酸的制备方法
公开(公告)号：US20100261233A1
公开(公告)日：2010-10-14
申请号：US12249092
申请日：2008-10-10
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C12P13/08
CPC分类号： C12N15/52 , C07K14/34 , C12P13/08
摘要： L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译：具有增强的lysE基因（赖氨酸出口载体基因）的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因）的lys附加基因，lysC基因（天冬氨酸激酶基因），dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）增强，特别是过表达，以及制备L-赖氨酸的方法。

34. 发明申请

US20070054380A1 L-LYSINE-PRODUCING CORYNEBACTERIA AND PROCESS FOR THE PREPARATION OF L-LYSINE 有权
标题翻译：生产L-赖氨酸的L-赖氨酸和L-赖氨酸的制备方法
公开(公告)号：US20070054380A1
公开(公告)日：2007-03-08
申请号：US11425091
申请日：2006-06-19
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C12P13/08 , C07H21/04 , C12N15/74 , C12N1/21
CPC分类号： C12N15/52 , C07K14/34 , C12P13/08
摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译：本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因），dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

35. 发明授权

US07094584B2 L-Lysine-producing corynebacteria and process for the preparation of L-lysine 有权
标题翻译： L-赖氨酸生产性棒状杆菌和L-赖氨酸的制备方法
公开(公告)号：US07094584B2
公开(公告)日：2006-08-22
申请号：US10804120
申请日：2004-03-19
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Möckel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Möckel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C12P13/08
CPC分类号： C12N15/52 , C07K14/34 , C12P13/08
摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译：本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因），dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

36. 发明授权

US07078502B2 Nucleotide sequences for encoding of the lysR2-gene 失效
标题翻译：编码lysR2基因的核苷酸序列
公开(公告)号：US07078502B2
公开(公告)日：2006-07-18
申请号：US09826909
申请日：2001-04-06
申请人： Bettina Moeckel , Mike Farwick , Thomas Hermann , Caroline Kreutzer , Walter Pfefferle
发明人： Bettina Moeckel , Mike Farwick , Thomas Hermann , Caroline Kreutzer , Walter Pfefferle
IPC分类号： C07H21/02 , C07H21/04 , C12N15/00 , C12N15/09 , C12N15/63
CPC分类号： C12P13/08 , C07K14/34
摘要： Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
摘要翻译：分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸，b）多核苷酸，其编码多肽，其包含与SEQ ID No.2的氨基酸序列至少70％的程度相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少lysR2基因存在于减毒形式，以及使用多核苷酸序列作为杂交探针。

37. 发明申请

US20050003423A1 Nucleotide sequences which code for the lysR3 gene 审中-公开
标题翻译：编码lysR3基因的核苷酸序列
公开(公告)号：US20050003423A1
公开(公告)日：2005-01-06
申请号：US10884166
申请日：2004-07-06
申请人： Bettina Moeckel , Caroline Kreutzer
发明人： Bettina Moeckel , Caroline Kreutzer
IPC分类号： C07K14/34 , C12N1/21 , C12N15/31 , C12P13/08 , C12Q1/68 , C07H21/04 , C12N9/22
CPC分类号： C07K14/34 , C12P13/08
摘要： The present invention relates to polynucleotides corresponding to the lysR3 gene and which encode a LysR3 transcriptional regulator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LysR3 transcriptional regulator activity.
摘要翻译：本发明涉及对应于lysR3基因并编码LysR3转录调节剂的多核苷酸，产生L-氨基酸的方法，以及筛选编码具有LysR3转录调节活性的蛋白质的多核苷酸的方法。

38. 发明授权

US06838267B2 Nucleotide sequences coding for the ccpA1 gene 失效
标题翻译：编码ccpA1基因的核苷酸序列
公开(公告)号：US06838267B2
公开(公告)日：2005-01-04
申请号：US09938540
申请日：2001-08-27
申请人： Bettina Moeckel , Caroline Kreutzer
发明人： Bettina Moeckel , Caroline Kreutzer
IPC分类号： C07K14/34 , C12N1/21 , C12P13/08 , C12P13/04
CPC分类号： C07K14/34 , C12P13/08
摘要： The present invention provides isolated polynucleotides containing a polynucleotide sequence which is: a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, or b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, or c) polynucleotide which is complementary to the polynucleotides of a) or b), or d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the ccpA1 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

39. 发明授权

US06689586B2 Nucleotide sequences which code for the CcpA2 gene 失效
标题翻译：编码CcpA2基因的核苷酸序列
公开(公告)号：US06689586B2
公开(公告)日：2004-02-10
申请号：US09938642
申请日：2001-08-27
申请人： Bettina Moeckel , Caroline Kreutzer , Thomas Hermann , Mike Garwick , Achim Marx , Walter Pfefferle
发明人： Bettina Moeckel , Caroline Kreutzer , Thomas Hermann , Mike Garwick , Achim Marx , Walter Pfefferle
IPC分类号： C12P2106
CPC分类号： C07K14/34 , C12P13/08
摘要： The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.
摘要翻译：本发明涉及对应于ccpA2基因并且编码CcpA2分解代谢物控制蛋白质的多核苷酸，产生L-氨基酸的方法，以及筛选编码具有CcpA2分解代谢物控制活性的蛋白质的多核苷酸的方法。

40. 发明申请

US20080293100A1 Method for the Fermentative Production of L-Amino Acids With the Aid of Coryneform Bacteria Capable of Using Glycerin as the Only Carbon Source 有权
标题翻译：使用能够使用甘油作为唯一碳源的棒状杆菌细菌的发酵L-氨基酸的发酵方法
公开(公告)号：US20080293100A1
公开(公告)日：2008-11-27
申请号：US11816539
申请日：2006-09-27
申请人： Volker F. Wendisch , Doris Rittmann , Hermann Sahm , Caroline Kreutzer
发明人： Volker F. Wendisch , Doris Rittmann , Hermann Sahm , Caroline Kreutzer
IPC分类号： C12P13/08 , C12N1/21 , C12P13/22
CPC分类号： C12N1/20 , C12P13/04 , C12P13/08 , C12P13/227
摘要： The invention relates to a method for producing L-amino acids in which the following steps are carried out: a) the recombinant coryneform bacteria which produce the desired L-amino acid and in which at least one or several of the heterologous polynucleotides of the glycerol metabolism, selected among the group comprising glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, glpT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and talC, is/are expressed are cultivated in a medium containing glycerol or one or several other optional C sources in conditions in which the desired L-amino acid is enriched in the medium or the cells; and, optionally, b) the desired L-amino acid is isolated, all or fractions (>0 to 100 percent) of the components of the fermentation broth and/or biomass optionally remaining in the final product. In said method, bacteria are used in which other genes of the biosynthesis pathway of the desired L-amino acid are additionally reinforced or in which the metabolism pathways reducing the formation of the desired L-amino acid are eliminated at least in part.
摘要翻译：本发明涉及一种生产L-氨基酸的方法，其中进行以下步骤：a）产生所需L-氨基酸的重组棒状细菌，其中至少一种或几种甘油的异源多核苷酸被表达为包括glpA，glpB，glpC，glpD，glpE，glpF，glpG，glpK，glpQ，glpG，glpX，gldA，dhaK，dhaL，dhaM，dhaR，fsa和talC的组中的代谢在含有所需L-氨基酸在培养基或细胞中富集的条件下，在含有甘油或一种或几种其它任选的C源的培养基中; 和任选地，b）分离所需的L-氨基酸，所有或发酵液和/或生物质组分的组分（> 0至100％）任选保留在最终产物中。在所述方法中，使用细菌，其中所需L-氨基酸的生物合成途径的其它基因被额外增强，或者至少部分地消除了减少所需L-氨基酸形成的代谢途径。

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