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    • 30. 发明授权
    • Starch-degrading enzymes derived from cladosporium resinae
    • 淀粉降解酶衍生自枝孢菌
    • US4234686A
    • 1980-11-18
    • US46482
    • 1979-06-07
    • James J. Marshall
    • James J. Marshall
    • C12C5/00C12C12/02C12N9/26C12N9/28C12N9/34C12N9/44C12P19/14C12P19/24C12P19/16C12P19/20
    • C12N9/2428C12C12/02C12C5/004C12N9/2417C12N9/242C12N9/2457C12P19/14C12P19/24C12Y302/01041
    • A culture filtrate of Cladosporium resinae (Strain ATCC No. 20495) has been found to contain a mixture of starch-degrading enzymes capable of bringing about efficient conversion of starch and pullulan into glucose. Culture conditions resulting in optimal production of the pullulan degrading activity been established. The amylolytic enzyme preparation obtained by culturing the fungus under these optimal conditions has been fractionated by ion-exchange and molecular sieve chromatography and shown to contain at least four enzymes, a maltase, .alpha.-amylase and two glucoamylase-type enzymes including a novel exo-pullulanase. The maltase and glucoamylase enzymes have been purified to homogeneity and their substrate specificity investigated. Both the mixture and the exo-pullulanase can be used in the manufacture of dextrose from starch. Alone or in conjunction with appropriate other starch-degrading enzymes the mixture or the exo-pullulanase may be used in the production of specific starch conversion products, including fructose syrups. Both the mixture and the exo-pullanase are useful in increasing the fermentability of high DE starch conversion products and may be useful in the production of low calorie alcoholic beverages.
    • 已发现Cladosporium resinae(菌株ATCC No.20495)的培养滤液含有能够使淀粉和支链淀粉有效转化为葡萄糖的淀粉降解酶的混合物。 确定了最佳生产支链淀粉降解活性的培养条件。 通过在这些最佳条件下培养真菌而获得的淀粉分解酶制剂已经通过离子交换和分子筛分色分级,并显示含有至少四种酶,麦芽糖酶,α-淀粉酶和两种葡糖淀粉酶型酶,包括新型外切酶, 支链淀粉酶。 已经将麦芽糖酶和葡糖淀粉酶纯化至均匀,并研究其底物特异性。 混合物和外支链淀粉酶都可用于从淀粉制造葡萄糖。 单独或与适当的其它淀粉降解酶联合,混合物或外支链淀粉酶可用于生产特定淀粉转化产物,包括果糖糖浆。 混合物和外切拉酸酶都可用于增加高DE淀粉转化产物的发酵能力,并且可用于生产低热量酒精饮料。