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1. 发明授权

US4560651A Debranching enzyme product, preparation and use thereof 失效
标题翻译：提取酶产物，其制备和用途
公开(公告)号：US4560651A
公开(公告)日：1985-12-24
申请号：US434745
申请日：1982-10-18
申请人： Grethe C. Nielsen , Ivan V. Diers , Helle Outtrup , Barrie E. Norman
发明人： Grethe C. Nielsen , Ivan V. Diers , Helle Outtrup , Barrie E. Norman
IPC分类号： C12N9/24 , C12N9/34 , C12N9/44 , C12P19/16 , C12P19/22 , C12N1/20 , C12P19/20 , C12R1/07
CPC分类号： C12Y302/01003 , C12N9/2428 , C12P19/16 , Y10S435/832
摘要： A novel debranching enzyme product with properties in terms of thermostability and pH-optimum comparable to those of glucoamylase is produced by cultivating a strain belonging to the novel taxonomic group Bacillus acidopullulyticus. The novel debranching enzyme is used in conjunction with known saccharifying enzymes for the hydrolysis of starch.
摘要翻译：通过培育属于新型分类学克拉酸杆菌溶血素菌株的菌株，产生了与热葡萄糖淀粉酶相当的热稳定性和pH-最佳性质的新型脱支酶产物。新型脱支酶与已知的用于水解淀粉的糖化酶结合使用。

2. 发明授权

US4970158A Beta amylase enzyme product, preparation and use thereof 失效
标题翻译： β-淀粉酶产物，其制备及应用
公开(公告)号：US4970158A
公开(公告)日：1990-11-13
申请号：US15239
申请日：1987-02-17
申请人： Helle Outtrup , Barrie E. Norman
发明人： Helle Outtrup , Barrie E. Norman
IPC分类号： C12N1/20 , C12N9/26 , C12P19/22 , C12R1/07
CPC分类号： C12N9/2425 , C12P19/22 , Y10S435/832
摘要： A novel beta amylase enzyme product with improved thermostability and acid-stability is provided. The novel enzyme can be produced by cultivating a newly discovered microorganism Bacillus sp. (21-51) belonging to the Bacillus acidopullulyticus complex and may be used alone or in combination with a variety of conventional debanching enzymes in the production of high maltose syrups.
摘要翻译：提供了具有改善的热稳定性和酸稳定性的新型β-淀粉酶产物。该新型酶可以通过培养新发现的微生物芽孢杆菌（21-51），其可以单独使用或与多种常规脱皮酶组合使用，用于生产高麦芽糖浆。

3. 发明授权

US5234823A Chimeric enzymes 失效
标题翻译：嵌合酶
公开(公告)号：US5234823A
公开(公告)日：1993-08-10
申请号：US607798
申请日：1990-10-26
申请人： Borge K. Diderichsen , Helle Outtrup , Martin Shulein , Barrie E. Norman
发明人： Borge K. Diderichsen , Helle Outtrup , Martin Shulein , Barrie E. Norman
IPC分类号： C12N9/28 , C12P19/14
CPC分类号： C12N9/2417 , C12P19/14
摘要： Chimeric alpha-amylases are disclosed of the formulaQ--R--Lin which Q is a N-terminal polypeptide residue of from 55 to 60 amino acid residues which is at least 75 percent homologous to the 55 N-terminal amino acid residues in the Bacillus amyloliquefaciens alpha-amylase as described in Takkinen et al., J. Biol. Chem. 258 (1983) 1007-1013,R is a polypeptide residue of the general formula ##STR1## L comprises a C-terminal polypeptide of from 390 to 400 amino acid residues which is at least 75 percent holologous to the 395 C-terminal amino acid residues in the Bacillus licheniformis 584 (ATCC 27811) alpha-amylase.
摘要翻译：公开了式QRL的嵌合α-淀粉酶，其中Q是55至60个氨基酸残基的N-末端多肽残基，其与解淀粉芽孢杆菌α-淀粉酶杆菌中的55个N-末端氨基酸残基至少75％同源如Takkinen等人，J.Biol.Chem。 Chem。 258（1983）1007-1013中，R是通式Pro-Tyr-Asp-Leu-Tyr-Asp-Leu-Gly-Glu-Phe-X8-Gln-Lys-Gly-Thr-Val-Arg的多肽残基 -Thr-Lys-Tyr-Gly-Thr-Lys-X9-Glu-Leu-Gln-X10-Ala-Ile-Lys。 L包含390至400个氨基酸残基的C末端多肽，其与地衣芽孢杆菌584（ATCC 27811）α-淀粉酶中的395个C-末端氨基酸残基具有至少75％的全息。

4. 发明申请

US20120283164A1 Alpha-Amylase Mutants 审中-公开
标题翻译： α-淀粉酶突变体
公开(公告)号：US20120283164A1
公开(公告)日：2012-11-08
申请号：US13556623
申请日：2012-07-24
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Neilsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Neilsen , Lisbeth Hedegaard
IPC分类号： C12N9/26 , C11D3/386
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译：本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。

5. 发明授权

US07951574B2 Subtilases 失效
公开(公告)号：US07951574B2
公开(公告)日：2011-05-31
申请号：US12957623
申请日：2010-12-01
申请人： Preben Nielsen , Poul Erik Pedersen , Helle Outtrup
发明人： Preben Nielsen , Poul Erik Pedersen , Helle Outtrup
IPC分类号： C12N9/54 , C12N15/57 , C12N15/62 , C12N15/74 , C12N15/75 , C11D3/386
CPC分类号： C12N15/62 , C12N9/54 , C12N15/52
摘要： The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.

6. 发明申请

US20080318271A1 Subtilases 失效
公开(公告)号：US20080318271A1
公开(公告)日：2008-12-25
申请号：US11575534
申请日：2005-09-21
申请人： Preben Nielsen , Poul Erik Pedersen , Helle Outtrup
发明人： Preben Nielsen , Poul Erik Pedersen , Helle Outtrup
IPC分类号： C12P21/00 , C12N9/14 , C07H21/04 , C12N15/63 , C12N1/21
CPC分类号： C12N15/62 , C12N9/54 , C12N15/52
摘要： The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.

7. 发明申请

US20080280325A1 Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same 审中-公开
标题翻译：具有内切葡聚糖酶活性的多肽和编码相同的多核苷酸
公开(公告)号：US20080280325A1
公开(公告)日：2008-11-13
申请号：US12090400
申请日：2006-11-15
申请人： Katja Salomon Johansen , Keith Gibson , Preben Nielsen , Helle Outtrup
发明人： Katja Salomon Johansen , Keith Gibson , Preben Nielsen , Helle Outtrup
IPC分类号： C12P21/04 , C12N9/42 , C11D7/42 , C12N15/11 , C12N15/00 , C12N1/20
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004
摘要： The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有内切葡聚糖酶活性的分离多肽和编码多肽的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。

8. 发明授权

US06528298B1 &agr;-amylase mutants 有权
标题翻译： α-淀粉酶突变体
公开(公告)号：US06528298B1
公开(公告)日：2003-03-04
申请号：US09545586
申请日：2000-04-07
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
IPC分类号： C12P2106
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译：本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。

9. 发明授权

US06309871B1 Polypeptides having alkaline &agr;-amylase activity 有权
标题翻译：具有碱性α-淀粉酶活性的多肽
公开(公告)号：US06309871B1
公开(公告)日：2001-10-30
申请号：US09291023
申请日：1999-04-13
申请人： Helle Outtrup , Torben Vedel Borchert , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard Hoeck
发明人： Helle Outtrup , Torben Vedel Borchert , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard Hoeck
IPC分类号： C12N928
CPC分类号： C12N9/2417 , C11D3/386
摘要： The present invention relates to isolated polypeptides having &agr;-amylase activity and isolated nucleic acid sequences encoding the polypeptides, which may be derived from Bacillus. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有α-淀粉酶活性的分离的多肽和编码可衍生自芽孢杆菌的多肽的分离的核酸序列。本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

10. 发明授权

US06280984B1 DNA constructs and methods of producing cellulytic enzymes 失效
标题翻译： DNA构建体和产生纤维素分解酶的方法
公开(公告)号：US06280984B1
公开(公告)日：2001-08-28
申请号：US09226529
申请日：1999-01-07
申请人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisgård-Frantzen , Martin Schülein , Per Linaa Jorgensen , Mads E. Bjoernvad
发明人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisgård-Frantzen , Martin Schülein , Per Linaa Jorgensen , Mads E. Bjoernvad
IPC分类号： C12P1904
CPC分类号： C12Y302/01032 , C11D3/386 , C11D3/38636 , C11D3/38645 , C12N9/2437 , C12N9/248 , C12N9/54 , C12R1/07 , C12Y302/01004 , C12Y302/01008 , D21C5/005 , Y10S435/832
摘要： An isolated nucleic acid constructs encoding cellulytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining cellulytic enzymes.
摘要翻译：编码衍生自芽孢杆菌属芽孢杆菌菌株的重组载体和包含这些构建体的宿主细胞的分离的核酸构建体和获得纤维素分解酶的方法。

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