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21. 发明申请

US20150252377A1 GENES CONTROLLING PHOTOPERIOD SENSITIVITY IN MAIZE AND SORGHUM AND USES THEREOF 审中-公开
标题翻译：控制玉米和荞麦光合作用敏感性的基因及其用途
公开(公告)号：US20150252377A1
公开(公告)日：2015-09-10
申请号：US14431386
申请日：2013-10-01
申请人： Olga N. DANILEVSKAYA , Xin MENG , PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY
发明人： Nathan David Coles , Olga Nikolaevna Danilevskaya , Xin Meng
IPC分类号： C12N15/82 , C07K14/415 , C12Q1/68
CPC分类号： C12N15/8222 , C07K14/415 , C12N15/827 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： The present disclosure provides polynucleotide sequences controlling the photoperiod sensitive trait in maize. The disclosure provides polynucleotide sequences and the use of encoded polypeptides associated with the photoperiod sensitivity. The disclosed sequences are responsible for controlling plant growth, source-sink relationships and yield in crop plants.
摘要翻译：本公开提供控制玉米光周期敏感性状的多核苷酸序列。本公开提供多核苷酸序列和与光周期灵敏度相关的编码多肽的用途。所公开的序列负责控制作物植物的植物生长，源 - 宿关系和产量。

22. 发明申请

US20150082481A1 Nucleic acid molecules and other molecules associated with transcription in plants and uses thereof for plant improvement 审中-公开
标题翻译：核酸分子和与植物转录相关的其他分子及其用于植物改良的用途
公开(公告)号：US20150082481A1
公开(公告)日：2015-03-19
申请号：US14480199
申请日：2014-09-08
申请人： MONSANTO TECHNOLOGY LLC
发明人： Thomas J. LA ROSA , Linda LUTFIYYA , Yihua ZHOU , David K. KOVALIC , Andrey A. BOUKHAROV , Ping LI , Wei WU , Timothy W. CONNER , Jingdong LIU
IPC分类号： C12N15/82
CPC分类号： C12N15/8218 , C07K14/415 , C12N15/8201 , C12N15/8222 , C12N15/8242 , C12N15/8261 , C12N15/827 , C12N15/8273 , Y02A40/146
摘要： Polynucleotides useful for improvement of plants are provided. In particular, polynucleotide sequences are provided from plant sources. Polypeptides encoded by the polynucleotide sequences are also provided. The disclosed polynucleotides and polypeptides find use in production of transgenic plants to produce plants having improved properties.
摘要翻译：提供了可用于改良植物的多核苷酸。特别地，从植物来源提供多核苷酸序列。还提供了由多核苷酸序列编码的多肽。所公开的多核苷酸和多肽可用于生产转基因植物以产生具有改进性质的植物。

23. 发明授权

US08957282B2 Yield and stress tolerance in transgenic plants IV 有权
标题翻译：转基因植物的产量和胁迫耐受性
公开(公告)号：US08957282B2
公开(公告)日：2015-02-17
申请号：US12750997
申请日：2010-03-31
申请人： Robert A. Creelman , Neal I. Gutterson , Oliver J. Ratcliffe , T. Lynne Reuber , R. Eric Cerny , Kimberly Faye Zobrist Duff , Susanne Kjemtrup-Lovelace , Robert J. Meister , Marie E. Petracek , Thomas Ruff , Qingzhang Xu
发明人： Robert A. Creelman , Neal I. Gutterson , Oliver J. Ratcliffe , T. Lynne Reuber , R. Eric Cerny , Kimberly Faye Zobrist Duff , Susanne Kjemtrup-Lovelace , Robert J. Meister , Marie E. Petracek , Thomas Ruff , Qingzhang Xu
IPC分类号： C12N15/82 , C12N15/29 , A01H1/02 , C07K14/415
CPC分类号： C12N15/8273 , C07K14/415 , C12N15/00 , C12N15/8261 , C12N15/8262 , C12N15/827 , C12N15/8271 , Y02A40/146
摘要： Polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The polypeptides of the invention have been shown to confer at least one regulatory activity and confer increased yield, greater height, greater early season growth, greater canopy coverage, greater stem diameter, greater late season vigor, increased secondary rooting, more rapid germination, greater cold tolerance, greater tolerance to water deprivation, reduced stomatal conductance, altered C/N sensing, increased low nitrogen tolerance, increased low phosphorus tolerance, or increased tolerance to hyperosmotic stress as compared to the control plant as compared to a control plant.
摘要翻译：掺入表达载体的多核苷酸和多肽已经被引入植物中并被异位表达。本发明的多肽已显示赋予至少一种调节活性，并且赋予增加的产量，更高的高度，更大的早季生长，更大的冠层覆盖率，更大的茎直径，更大的晚季活力，增加的二次生根，更迅速的发芽，更大的与对照植物相比，与对照植物相比，耐寒性，对水分缺乏的更大耐受性，降低的气孔导度，改变的C / N感测，增加的低氮耐受性，增加的低磷耐受性或增加的耐渗透胁迫。

24. 发明申请

US20140366221A1 Methods of Controlling Seed Size in Plants 有权
标题翻译：控制植物种子大小的方法
公开(公告)号：US20140366221A1
公开(公告)日：2014-12-11
申请号：US14372582
申请日：2013-01-15
申请人： Institute of Genetics and Developmental Biology
发明人： Yunhai Li , Wenjuan Fang , Zhibiao Wang , Rongfeng Cui
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N9/0071 , C12N9/0077 , C12N15/8218 , C12N15/827 , Y02A40/146
摘要： This invention relates to the identification of a regulator protein (termed CYP78A6, or EOD3) which controls the size of plant seeds and organs in Arabidopsis and other plants. Manipulation of CYP78A protein expression may useful, for example, in improving crop yield and increasing plant biomass.
摘要翻译：本发明涉及在拟南芥和其他植物中控制植物种子和器官大小的调节蛋白（称为CYP78A6或EOD3）的鉴定。操作CYP78A蛋白表达可用于例如改善作物产量和增加植物生物量。

25. 发明申请

US20140366213A1 MODIFYING FLOWERING TIME IN MAIZE 审中-公开
标题翻译：修改花期时间
公开(公告)号：US20140366213A1
公开(公告)日：2014-12-11
申请号：US14296352
申请日：2014-06-04
申请人： Wisconsin Alumni Research Foundation
发明人： Shawn Kaeppler , Natalia de Leon , Jillian M. Foerster , German Muttoni
IPC分类号： C12N15/82 , C12N15/01 , A01H1/02 , C07K14/415
CPC分类号： C12N15/827 , C07K14/415
摘要： The present invention provides compositions and methods for modulating the reproductive transition in plants, such as grasses (e.g., maize). In particular, the invention provides methods for enhancing agronomic properties in plants by modulating expression of GRMZM2G171650 (zmm22) or homologs thereof. Modulation of expression of one or more additional genes which affect reproductive transition such as zaglI, in conjunction with such modulation of expression is also contemplated. Nucleic acid constructs for down-regulation of GRMZM2G171650 are also contemplated, as are transgenic plants, and products produced therefrom, that demonstrate altered development such as extended flowering time and display associated phenotypes such as enhanced yield of vegetative biomass, improved digestibility, and increased disease resistance. Plants described herein may be used, for example, as improved forage or feed crops or in biofuel production.
摘要翻译：本发明提供用于调节植物如禾本科植物（例如玉米）中的繁殖转化的组合物和方法。特别地，本发明提供了通过调节GRMZM2G171650（zmm22）或其同系物的表达来增强植物中农艺性质的方法。还考虑了与这种表达调节一起调节影响生殖过渡如zaglI的一种或多种另外的基因的表达。还考虑了用于下调GRMZM2G171650的核酸构建体，以及转基因植物及其产生的产物，其表现出改变的发育，例如延长的开花时间和显示相关表型，例如增加营养生物量的产量，改善的消化率和增加的疾病抵抗性。本文所述的植物可以用于例如改良的饲料作物或饲料作物或生物燃料生产。

26. 发明授权

US08877435B2 Methods and means for producing efficient silencing construct using recombinational cloning 有权
标题翻译：使用重组克隆产生高效沉默构建体的方法和手段
公开(公告)号：US08877435B2
公开(公告)日：2014-11-04
申请号：US12886905
申请日：2010-09-21
申请人： Christopher A. Helliwell , Susan V. Wesley , Peter M. Waterhouse
发明人： Christopher A. Helliwell , Susan V. Wesley , Peter M. Waterhouse
IPC分类号： C12Q1/68 , C12P19/34 , C12N15/00 , C12N15/87 , C07H21/02
CPC分类号： C12N15/825 , C12N15/10 , C12N15/63 , C12N15/8218 , C12N15/8249 , C12N15/827
摘要： Methods and means are provided for producing chimeric nucleic acid constructs capable of producing dsRNA for silencing target nucleic acid sequences of interest using recombinational cloning.
摘要翻译：提供了用于产生能够使用重组克隆使dsRNA沉默目的核酸序列沉淀的嵌合核酸构建体的方法和手段。

27. 发明申请

US20140230088A1 GENE SILENCING 审中-公开
标题翻译：基因沉默
公开(公告)号：US20140230088A1
公开(公告)日：2014-08-14
申请号：US14264275
申请日：2014-04-29
申请人： E I DU PONT DE NEMOURS AND COMPANY
发明人： MILO AUKERMAN
IPC分类号： C12N15/82
CPC分类号： C12N15/8201 , C12N15/8216 , C12N15/8218 , C12N15/827
摘要： The invention provides methods and compositions useful in target sequence suppression and target sequence validation. The invention provides polynucleotide constructs useful for gene silencing, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence.
摘要翻译：本发明提供了可用于靶序列抑制和靶序列验证的方法和组合物。本发明提供了可用于基因沉默的多核苷酸构建体，以及包含多核苷酸的细胞，植物和种子。本发明还提供了使用微小RNA沉默靶序列的方法。

28. 发明申请

US20140201864A1 Polynucleotides and Polypeptides in Plants 审中-公开
标题翻译：植物中的多核苷酸和多肽
公开(公告)号：US20140201864A1
公开(公告)日：2014-07-17
申请号：US14177551
申请日：2014-02-11
申请人： Mendel Biotechnology, Inc.
发明人： Roderick W. Kumimoto , Luc J. Adam , Roger Canales , Karen S. Century , Robert A. Creelman , Jennifer M. Costa , Neal I. Gutterson , Frederick D. Hempel , Jacqueline E. Heard , Cai-Zhong Jiang , Katherine Krolikowski , Omaira Pineda , Emily L. Queen Kumimoto , Oliver J. Ratcliffe , Peter P. Repetti , T. Lynne Reuber , Jose Luis Riechmann , James Z. Zhang
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8214 , C12N15/8245 , C12N15/8247 , C12N15/8249 , C12N15/825 , C12N15/8251 , C12N15/8261 , C12N15/8262 , C12N15/8266 , C12N15/8267 , C12N15/827 , C12N15/8271 , C12N15/8273 , C12N15/8275 , C12N15/8279 , C12N15/8281 , C12N15/8282 , C12N15/8287 , C12N15/8291 , C12N15/8293 , Y02A40/146
摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
摘要翻译：本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同系物，以及使用多核苷酸和多肽产生与参照植物相比具有有利特性的转基因植物的方法。与这些多核苷酸和多肽相关的序列信息也可以用于生物信息学检索方法，并且也被公开。

29. 发明申请

US20140196160A1 Methods and Means for Monitoring and Modulating Gene Silencing 审中-公开
标题翻译：监测和调控基因沉默的方法和手段
公开(公告)号：US20140196160A1
公开(公告)日：2014-07-10
申请号：US14100272
申请日：2013-12-09
申请人： Commonwealth Scientific & Industrial Research Organisation
发明人： Peter Waterhouse , Susan Wesley , Chris Helliwell
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A61K48/00 , C07K2319/00 , C12N15/8218 , C12N15/825 , C12N15/827 , C12N2310/14 , C12N2310/53
摘要： Methods and means are provided for monitoring and modulating reduction of gene expression in eukaryotic organisms, using double stranded RNA comprising, in addition to the dsRNA region comprising nucleotide sequences homologous to the target gene, additional dsRNA regions designed to down regulate a second gene or which are unrelated to the target gene.
摘要翻译：提供了用于监测和调节真核生物体中基因表达降低的方法和方法，使用双链RNA，除了包含与靶基因同源的核苷酸序列的dsRNA区域外，还设计了用于下调第二基因的其它dsRNA区域，或者与靶基因无关。

30. 发明授权

US08729339B2 Gene silencing 有权
标题翻译：基因沉默
公开(公告)号：US08729339B2
公开(公告)日：2014-05-20
申请号：US11958423
申请日：2007-12-18
申请人： Milo J. Aukerman
发明人： Milo J. Aukerman
IPC分类号： C12N15/82 , C07H21/04
CPC分类号： C12N15/8201 , C12N15/8216 , C12N15/8218 , C12N15/827
摘要： The invention provides methods and compositions useful in target sequence suppression and target sequence validation. The invention provides polynucleotide constructs useful for gene silencing, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence.
摘要翻译：本发明提供了可用于靶序列抑制和靶序列验证的方法和组合物。本发明提供了可用于基因沉默的多核苷酸构建体，以及包含多核苷酸的细胞，植物和种子。本发明还提供了使用微小RNA沉默靶序列的方法。

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