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    • 22. 发明申请
    • Method of immobilizing a protein to a zeolite
    • 将蛋白质固定在沸石上的方法
    • US20060240567A1
    • 2006-10-26
    • US10534162
    • 2003-11-07
    • Stanley BrownRune WendelboSanne Nygaard
    • Stanley BrownRune WendelboSanne Nygaard
    • G01N33/558
    • C07K17/14C07K14/001C07K19/00C07K2319/00
    • The present invention relates to a polypeptide tag and a method using said polypeptide tag sequence for immobilizing a protein on a microporous material, said microporous material is selected from the group consisting of zeolite or similar solid surfaces whereby loss of activity of said protein is less than 10% of the initial activity prior to immobilization, the method comprising the steps of: 1. Selecting a polypeptide tag capable of binding to the surface, 2. Immobilizing said protein by the steps of: attaching said polypeptide tag to the protein, and binding said polypeptide tag to the solid surface where step (a) and (b) is performed simultaneously or sequentially and when performed sequentially, the order of step (a) and (b) is random, subject to the limitation that the polypeptide tag does not consist only of histidine residues.
    • 本发明涉及多肽标签和使用所述多肽标签序列将蛋白质固定在微孔材料上的方法,所述微孔材料选自沸石或类似的固体表面,由此所述蛋白质的活性丧失小于 10%的固定前的初始活性,所述方法包括以下步骤:1.选择能够结合表面的多肽标签; 2.通过以下步骤固定所述蛋白质:将所述多肽标签连接到蛋白质上,并结合 所述多肽标签到固体表面,其中步骤(a)和(b)同时或顺序地进行,并且当顺序进行时,步骤(a)和(b)的顺序是随机的,但受限于多肽标签不 仅由组氨酸残基组成。
    • 26. 发明授权
    • Procedure for synthesis of crystalline microporous
silico-alumino-phosphates
    • 合成结晶微孔硅铝磷酸盐的方法
    • US5663471A
    • 1997-09-02
    • US256069
    • 1994-08-25
    • Steinar KvisleRune WendelboHanne ren
    • Steinar KvisleRune WendelboHanne ren
    • C01B33/26B01J29/00B01J29/85C01B25/36C01B25/45C01B37/08C01B39/10C01B39/54C07B61/00C07C1/20C07C11/02C10G3/00C07C1/00C01B39/00
    • C01B37/08B01J29/85C10G3/49C10G2400/20Y02P20/52Y02P30/20Y10S423/30
    • A process for producing crystalline microporous silico-alumino-phosphates with controlled Si content from a mixture of reactive sources of SiO.sub.2, Al.sub.2 O.sub.3 and P.sub.2 O.sub.5 and an organic template material, by combining at least a portion of the Al source and the P source in the absence of the Si source, mixing the resulting mixture with any remaining Al source, the Si source and the organic template material to obtain a gel, and heating the gel to obtain crystallization, wherein the Al and Si sources in the reaction mixture are mixed in the same mole ratios as in the crystalline microporous silica-alumino-phosphate to be produced, the P source is added in stoichiometric excess and hydrochloric acid is added at the same time as the P source, in a quantity such that the ratio of silica to hydrochloric acid in the gel is kept between 0.3 and 3. The invention also provides a crystalline microporous silico-alumino-phosphate produced by the process, and the use of such silico-alumino-phosphate as a catalyst for producing olefins from methanol.
    • PCT No.PCT / NO92 / 00193 Sec。 371日期:1994年8月25日 102(e)日期1994年8月25日PCT提交1992年12月9日PCT公布。 出版物WO93 / 13013 日期1993年7月8日由SiO 2,Al 2 O 3和P 2 O 5的反应性源和有机模板材料的混合物制备具有受控Si含量的结晶微孔硅铝磷酸盐的方法,通过将至少一部分Al源和 P源,在不存在Si源的情况下,将所得混合物与任何剩余的Al源,Si源和有机模板材料混合以获得凝胶,并加热凝胶以获得结晶,其中反应中的Al和Si源 混合物以与要生产的结晶微孔二氧化硅 - 铝磷酸盐相同的摩尔比混合,P源以化学计量过量加入,盐酸与P源同时加入,其量使得 凝胶中二氧化硅与盐酸的比例保持在0.3至3之间。本发明还提供了通过该方法制备的结晶微孔硅铝磷酸盐,并且使用这种硅铝酸盐 硫酸盐作为从甲醇生产烯烃的催化剂。