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21. 发明授权

US08889121B2 Bacterium comprising a regulated rfaH nucleic acid 有权
标题翻译：包含调节的rfaH核酸的细菌
公开(公告)号：US08889121B2
公开(公告)日：2014-11-18
申请号：US13574718
申请日：2011-01-21
申请人： Roy Curtiss, III , Qingke Kong
发明人： Roy Curtiss, III , Qingke Kong
IPC分类号： A01N63/00 , A61K39/00 , A61K39/02 , A61K39/112 , C07K14/315 , C07K14/255 , C12N9/10 , C12N1/36 , A61K39/09
CPC分类号： C07K14/3156 , A61K39/0275 , A61K39/092 , A61K2039/522 , A61K2039/523 , C07K14/255 , C12N1/36 , C12N9/1048 , Y02A50/47 , Y02A50/482
摘要： The present invention encompasses a recombinant bacterium comprising a regulated rfaH nucleic acid, as well as a vaccine comprising said recombinant bacterium. Other embodiments of the present invention encompass a recombinant bacterium comprising a regulated rfaH nucleic acid and a regulated rfc nucleic acid, while additional embodiments encompass a recombinant bacterium comprising a regulated rfaH nucleic acid and at least one nucleic acid encoding at least one exogenous antigen.
摘要翻译：本发明包括包含调节的rfaH核酸的重组细菌，以及包含所述重组细菌的疫苗。本发明的其它实施方案包括包含调节的rfaH核酸和调节的rfc核酸的重组细菌，而另外的实施方案包括包含调节的rfaH核酸和至少一种编码至少一种外源抗原的核酸的重组细菌。

22. 发明授权

US08465755B2 Recombinant bacterium capable of eliciting an immune response against enteric pathogens 有权
标题翻译：能够引起针对肠道病原体的免疫应答的重组细菌
公开(公告)号：US08465755B2
公开(公告)日：2013-06-18
申请号：US12681721
申请日：2008-10-06
申请人： Roy Curtiss, III , Melha Mellata , Bereket Zekarias , Zhaoxing Shi , Christine Branger , Kenneth Roland
发明人： Roy Curtiss, III , Melha Mellata , Bereket Zekarias , Zhaoxing Shi , Christine Branger , Kenneth Roland
IPC分类号： A61K39/112 , C12N1/21
CPC分类号： A61K39/0275 , A61K39/025 , A61K2039/522 , A61K2039/523 , C12N15/74 , Y02A50/47 , Y02A50/482 , Y02A50/484
摘要： The present invention relates to a recombinant bacterium that is capable of eliciting an immune response against at least two enteric pathogens, without substantially inducing an immune response specific to the serotype of the bacterium. The invention also relates to methods of making such a bacterium and vaccines and methods of using such a bacterium.
摘要翻译：本发明涉及能够引起针对至少两种肠道病原体的免疫应答而不基本上诱导对该细菌的血清型特异的免疫应答的重组细菌。本发明还涉及制备这种细菌和疫苗的方法以及使用这种细菌的方法。

23. 发明申请

US20120237987A1 BACTERIUM FOR PRODUCTION OF FATTY ACIDS 审中-公开
标题翻译：生产脂肪酸的细菌
公开(公告)号：US20120237987A1
公开(公告)日：2012-09-20
申请号：US13459774
申请日：2012-04-30
申请人： Roy Curtiss, III , Xinyao Liu
发明人： Roy Curtiss, III , Xinyao Liu
IPC分类号： C12N1/13 , C12P7/64
CPC分类号： C12P7/6409 , C12N1/20 , C12N1/38 , C12N9/16 , C12N15/74
摘要： The present invention encompasses a bacterium that produces fatty acids.
摘要翻译：本发明包括产生脂肪酸的细菌。

24. 发明申请

US20100317084A1 RECOMBINANT BACTERIA COMPRISING VECTORS FOR EXPRESSION OF NUCLEIC ACID SEQUENCES ENCODING ANTIGENS 审中-公开
标题翻译：包含用于表达编码抗原的核酸序列的载体的重组细菌
公开(公告)号：US20100317084A1
公开(公告)日：2010-12-16
申请号：US12599655
申请日：2008-05-09
申请人： Roy Curtiss, III
发明人： Roy Curtiss, III
IPC分类号： C12N1/21
CPC分类号： C12Y102/01011 , C12N9/0008 , C12N15/70 , C12N15/74
摘要： The invention encompasses a recombinant bacterium that comprises at least one vector capable of expressing a nucleic acid sequence encoding an antigen. In particular, the bacterium comprises at least one chromosomally encoded essential nucleic acid that is altered so that it is not expressed, and at least one extrachromosomal vector.
摘要翻译：本发明包括重组细菌，其包含至少一种能够表达编码抗原的核酸序列的载体。特别地，细菌包含至少一种经过改变以使其不被表达的染色体编码的必需核酸和至少一种染色体外载体。

25. 发明授权

US07341860B2 Regulated antigen delivery system (RADS) 有权
标题翻译：调节抗原递送系统（RADS）
公开(公告)号：US07341860B2
公开(公告)日：2008-03-11
申请号：US10924574
申请日：2004-08-24
申请人： Roy Curtiss, III , Steven A. Tinge
发明人： Roy Curtiss, III , Steven A. Tinge
IPC分类号： C12N1/20 , C12P21/06
CPC分类号： C12N15/74 , A61K2039/523 , A61K2039/53 , C12N15/635
摘要： We describe a regulated antigen delivery system (RADS) that has (a) a vector that includes (1) a gene encoding a desired gene product operably linked to a control sequence, (2) an origin of replication conferring vector replication using DNA polymerase III, and (3) an origin of replication conferring vector replication using DNA polymerase I, where the second origin of replication is operably linked to a control sequence that is repressible by a repressor. The RADS microorganism also has a gene encoding a repressor, operably linked to an activatible control sequence. The RADS described provide high levels of the desired gene product after repression of the high copy number origin of replication is lifted. The RADS are particularly useful as live bacterial vaccines. Also described is a delayed RADS system, in which there is a delay before the high copy number origin is expressed after the repression is lifted. The delayed RADS is also particularly useful for live bacterial vaccines. Also described are several control elements useful for these systems, as well as methods for providing immunity to a pathogen in a vertebrate immunized with the RADS microorganisms.
摘要翻译：我们描述了一种调控抗原递送系统（RADS），其具有（a）载体，其包含（1）编码与控制序列可操作地连接的所需基因产物的基因，（2）使用DNA聚合酶III赋予载体复制的复制起点，和（3）使用DNA聚合酶I赋予载体复制的复制起点，其中第二复制起点可操作地连接于由阻遏物阻抑的控制序列。 RADS微生物还具有编码阻抑物的基因，可操作地连接于活化的对照序列。所述的RADS提供高水平的所需基因产物，在抑制高拷贝数复制起点解除之后。 RADS作为活细菌疫苗是特别有用的。还描述了延迟的RADS系统，其中在压制被解除之后表示高拷贝数原点之前存在延迟。延迟RADS对活细菌疫苗也特别有用。还描述了可用于这些系统的若干控制元件，以及用于用RADS微生物免疫的脊椎动物中的病原体提供免疫的方法。

26. 发明授权

US5672345A Selective maintenance of a recombinant gene in a population of vaccine cells 失效
标题翻译：在疫苗细胞群中选择性维持重组基因
公开(公告)号：US5672345A
公开(公告)日：1997-09-30
申请号：US402308
申请日：1995-03-10
申请人： Roy Curtiss, III
发明人： Roy Curtiss, III
IPC分类号： C12N15/09 , A61K39/04 , C07K14/315 , C07K14/35 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/68 , C12R1/01 , C12R1/19 , C12R1/42 , C12R1/44 , A61K39/02 , C12P21/00
CPC分类号： C12N9/0008 , A61K39/04 , C07K14/315 , C07K14/35 , C12N15/00 , C12N15/68 , C12R1/42 , C12Y102/01011 , A61K2039/523
摘要： The invention encompasses methods of maintaining desired recombinant genes in a genetic population of cells expressing the recombinant gene. The methods utilize mutant cells which are characterized by a lack of a functioning native gene encoding an enzyme which is essential for cell survival, wherein this enzyme catalyzes a step in the biosynthesis of an essential cell wall structural component and the presence of a first recombinant gene encoding an enzyme which is a functional replacement for the native enzyme, wherein the first recombinant gene cannot replace the defective chromosomal gene. The first recombinant gene is structurally linked to a second recombinant gene encoding a desired product. Loss of the first recombinant gene causes the cells to lyse when the cells are in an environment where a product due to the expression of the first recombinant gene is absent. The invention also encompasses methods of creating and isolating mutant cells with the above characteristics. The cells of the invention are useful for commercial production of desired products, for components of vaccines for immunizing individuals, and for release into the environment.
摘要翻译：本发明包括在表达重组基因的细胞的遗传群体中维持所需重组基因的方法。该方法利用突变体细胞，其特征在于缺乏编码细胞存活所必需的酶的功能性天然基因，其中该酶催化必需细胞壁结构组分的生物合成和第一重组基因的存在编码作为天然酶的功能性替代物的酶，其中第一重组基因不能代替有缺陷的染色体基因。第一重组基因在结构上与编码所需产物的第二重组基因连接。当细胞处于不存在第一重组基因表达的产物的环境中时，第一重组基因的损失导致细胞裂解。本发明还包括产生和分离具有上述特征的突变细胞的方法。本发明的细胞可用于商业生产所需产品，用于免疫个体的疫苗的组分和用于释放到环境中。

27. 发明授权

US5654184A Oral immunization by transgenic plants 失效
公开(公告)号：US5654184A
公开(公告)日：1997-08-05
申请号：US458097
申请日：1995-06-01
申请人： Roy Curtiss, III , Guy A. Cardineau
发明人： Roy Curtiss, III , Guy A. Cardineau
IPC分类号： A01H5/00 , A61K39/00 , A61K39/002 , A61K39/02 , A61K39/09 , A61K39/12 , C07K14/005 , C07K14/195 , C07K14/245 , C07K14/315 , C07K14/41 , C07K19/00 , C12N5/10 , C12N9/10 , C12N9/24 , C12N15/09 , C12N15/31 , C12N15/82 , C12P21/02 , C12P21/08 , G01N33/569
CPC分类号： C12N9/1051 , C07K14/245 , C07K14/315 , C12N15/8258 , C12N9/16 , C12N9/2454 , C12Y301/03001 , C12Y302/01011 , A61K39/00 , C07K2319/00
摘要： The invention is directed to transgenic plants expressing colonization and/or virulence antigens specified by genes from pathogenic microorganisms. It is also directed to the use of such transgenic plants for oral immunization of humans and other animals to elicit a secretory immune response which inhibits colonization of or invasion by such pathogenic microorganisms through a mucosal surface of humans or other animals.

28. 发明授权

US4190495A Modified microorganisms and method of preparing and using same 失效
公开(公告)号：US4190495A
公开(公告)日：1980-02-26
申请号：US727365
申请日：1976-09-27
申请人： Roy Curtiss, III
发明人： Roy Curtiss, III
IPC分类号： C02F3/34 , C12N1/20 , C12N15/00 , C12N15/09 , C12P1/00 , C12R1/19 , C12K1/02
CPC分类号： C12N15/00 , Y02W10/37 , Y10S435/849
摘要： Microorganisms have been developed which may be characterized as possessing substantially all of the following qualities or capabilities:(a) capable of having foreign genetic information introduced thereinto and recovered therefrom along with its expression with production of useful gene products;(b) the microorganism being dependent for growth and survival upon defined conditions;(c) the microorganism being incapable of establishment or growth or colonization and/or survival under conditions or in ecological niches that are considered to be natural and/or undesirable for said microorganism;(d) the microorganism being capable of causing genetic information incorporated therein to undergo degradation under conditions or ecological niches that are considered to be natural and/or undesirable for said microorganism;(e) the microorganism being capable of permitting cloning vectors incorporated therein to be dependent for their replication, maintenance and/or function on said microorganism;(f) the microorganism being substantially incapable of transmitting cloning vectors or recombinant DNA molecules incorporated therein to other organisms under conditions or ecological niches that are considered to be natural and/or undesirable for said microorganism;(g) the microorganism being capable of being monitored by suitable means and/or techniques without substantial alteration of said microorganism; and(h) the microorganism being susceptible of substantially minimal contamination with other organisms when recombinant DNA molecules are incorporated therein and being substantially incapable of contaminating other organisms when incorporated therein or consumed thereby when recombinant DNA molecules are incorporated in said microorganism.Examples of such microorganisms are Escherichia coli K-12 .chi.1776, Escherichia coli K-12 .chi.1972, Escherichia coli K-12 .chi.1976 and Escherichia coli K-12 .chi.2076. Additionally, techniques have been developed and employed for imparting special properties, e.g. genetic properties, to microorganisms which render the resulting microorganisms unique. Also, techniques have been developed for the handling of plasmid and/or bacteriophage cloning DNA vectors for eventual insertion into microorganisms for testing therein, such as the above-mentioned microorganisms, and techniques have been developed for the transformation of microorganisms, such as the above-identified microorganisms, for the introduction of recombinant DNA molecules thereinto. Also, techniques have been developed in connection with the development or production of the above-identified microorganisms which impart special genetically-linked properties thereto, which techniques are applicable to a large number and diversity of microorganisms, including not only bacteria but also yeast and other cellular material.

29. 发明申请

US20160199467A1 REGULATED EXPRESSION OF ANTIGEN AND/OR REGULATED ATTENTUATION TO ENHANCE VACCINE IMMUNOGENICITY AND/OR SAFETY 有权
公开(公告)号：US20160199467A1
公开(公告)日：2016-07-14
申请号：US15040005
申请日：2016-02-10
申请人： Roy Curtiss, III , Shifeng Wang , Soo-Young Wanda , Wei Kong
发明人： Roy Curtiss, III , Shifeng Wang , Soo-Young Wanda , Wei Kong
IPC分类号： A61K39/00 , C12N15/74
CPC分类号： C12N15/74 , A61K35/74 , A61K39/00 , A61K2035/11 , A61K2039/522 , A61K2039/523 , C12N1/36
摘要： The invention relates to compositions and methods for making and using recombinant bacteria that are capable of regulated attenuation and/or regulated expression of one or more antigens of interest.

30. 发明授权

US09163219B2 Single expression vector for generation of a virus with a segmented genome 有权
标题翻译：用于产生具有分段基因组的病毒的单一表达载体
公开(公告)号：US09163219B2
公开(公告)日：2015-10-20
申请号：US12759842
申请日：2010-04-14
申请人： Roy Curtiss, III , Xiangmin Zhang
发明人： Roy Curtiss, III , Xiangmin Zhang
IPC分类号： C12N15/63 , C12N1/21 , C12N7/00 , C07K14/005 , C12N15/85
CPC分类号： C12N7/00 , C07K14/005 , C12N15/85 , C12N2760/16051 , C12N2760/16122 , C12N2760/16151 , C12N2800/103 , C12N2830/36 , C12N2840/20
摘要： The present invention encompasses an expression vector that is capable of generating a virus from a segmented genome. In particular, a single expression vector may be utilized to produce influenza virus in cultured cells. The expression vector can be delivered in a purified DNA form or by a suitably designed bacterial carrier to cells in culture or to animals. This invention increases the virus generation efficiency, which benefits vaccine development. The bacterial carrier harboring such a plasmid encoding an attenuated virus may be used as a vaccine against corresponding viral disease.
摘要翻译：本发明包括能够从分段基因组产生病毒的表达载体。特别地，单个表达载体可用于在培养的细胞中产生流感病毒。表达载体可以纯化的DNA形式或适当设计的细菌载体递送至培养物或动物细胞。本发明增加病毒生成效率，有利于疫苗开发。携带这种编码减毒病毒的质粒的细菌载体可以用作针对相应病毒性疾病的疫苗。

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