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21. 发明申请

US20100188741A1 LASER SCANNING MICROSCOPE 有权
标题翻译：激光扫描显微镜
公开(公告)号：US20100188741A1
公开(公告)日：2010-07-29
申请号：US12691185
申请日：2010-01-21
申请人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
发明人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
IPC分类号： G02B21/06
CPC分类号： G02B21/0032 , G02B21/0048 , G02B21/0076
摘要： A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.
摘要翻译：激光扫描显微镜具有照明光束路径和检测光束路径。提供了一种分束器，其在样品的方向上反射照明光并沿检测装置的方向透过检测光。提供了附加的分光器，用于反射照明光并传输检测光，该附加分光器被布置在照明光束路径中的第一分束器在照明方向下游，并且该附加分束器基本上透射在第一分束器和检测光，但是相对于其反射率获取与第一分束器基本不同的波长范围。

22. 发明授权

US07701632B2 Method and arrangement for changing the spectral composition and/or intensity of illumination light and/or specimen light in an adjustable manner 有权
标题翻译：以可调节的方式改变照明光和/或标本光的光谱组成和/或强度的方法和装置
公开(公告)号：US07701632B2
公开(公告)日：2010-04-20
申请号：US11833761
申请日：2007-08-03
申请人： Ralf Wolleschensky
发明人： Ralf Wolleschensky
IPC分类号： G02B21/06
CPC分类号： G01N15/1434 , G01J3/02 , G01J3/0224 , G01J3/0229 , G01J3/10 , G01J3/4406 , G01J3/447 , G01J2003/1282 , G01J2003/1286 , G01N21/6445 , G01N21/6458 , G01N2015/0065 , G01N2015/149 , G01N2015/1493 , G02B21/002
摘要： Method and arrangement for changing the spectral composition and/or intensity of illumination light and/or specimen light in an adjustable manner, wherein a spatial separation into radiation components of different polarization is carried out with a first polarizing device, a spectral, spatial splitting of at least one radiation component is carried out with first dispersion device, the polarization state of at least one part of the spectrally spatially split radiation component is changed, and a spatial separation and/or combination of radiation components of different polarization are/is carried out by a second polarizing device, wherein a spatial combination of radiation components which are changed and not changed with respect to their polarization state is advantageously carried out by a second dispersion device.
摘要翻译：用于以可调节的方式改变照明光和/或标本光的光谱组成和/或强度的方法和装置，其中利用第一偏振装置，光谱，空间分裂进行不同偏振的辐射分量的空间分离使用第一分散装置执行至少一个辐射分量，改变光谱空间分裂的辐射分量的至少一部分的偏振状态，并且执行不同极化的辐射分量的空间分离和/或组合通过第二偏振装置，其中通过第二分散装置有利地进行相对于它们的偏振状态改变而不改变的辐射分量的空间组合。

23. 发明授权

US07605976B1 Method and device for changing light in an adjustable manner 有权
标题翻译：用于以可调节的方式改变光的方法和装置
公开(公告)号：US07605976B1
公开(公告)日：2009-10-20
申请号：US11416455
申请日：2006-05-03
申请人： Ralf Wolleschensky , Michael Kempe , Peter Klopfleisch
发明人： Ralf Wolleschensky , Michael Kempe , Peter Klopfleisch
IPC分类号： G02B21/06
CPC分类号： G01J3/4406 , G01J3/02 , G01J3/021 , G01J3/0224 , G01J3/0229 , G01J3/14 , G01N21/21 , G01N21/6445 , G01N21/6458 , G01N2021/6417 , G02B21/0068 , G02B21/0076 , G02B26/0816 , G02B27/143 , G02B27/283
摘要： Method and device for changing the illumination light and/or specimen light with respect to its spectral composition and/or intensity in an adjustable manner, wherein a spatial separation into the radiation components of different polarization is carried out with the first polarization means (Pol 1), a spectral spatial splitting of at least one radiation component is carried out with the first dispersion means (Disp1), and the polarization state of at least one part of the spectrally spatially split radiation component is changed, wherein a reflection of the illumination light and/or the detection light is carried out.
摘要翻译：用于以可调节的方式改变照明光和/或样本光的方法和装置，其中利用第一偏振装置（Pol 1）执行不同偏振的辐射分量的空间分离），利用第一色散装置（Disp1）执行至少一个辐射分量的光谱空间分裂，并且改变光谱空间分裂的辐射分量的至少一部分的偏振状态，其中照明光的反射和/或检测光。

24. 发明申请

US20090257107A1 Device for controlling light radiation 有权
标题翻译：用于控制光辐射的装置
公开(公告)号：US20090257107A1
公开(公告)日：2009-10-15
申请号：US12318287
申请日：2008-12-23
申请人： Ralf Wolleschensky
发明人： Ralf Wolleschensky
IPC分类号： G02F1/11 , G02B21/06
CPC分类号： G02B27/283 , G01J3/0224 , G01J3/1256 , G01J3/4406 , G01J3/447 , G01N21/6445 , G01N21/6458 , G02B21/002 , G02B21/0068
摘要： Device for controlling light radiation, which is excited in a specimen and/or which is backscattered and/or reflected and which contains one or more wavelengths, at a plurality of light outlets, wherein a separation of the light radiation into differently polarized components is carried out; and the components of the excitation radiation and/or detection radiation are affected in their polarization by means of a preferably birefringent, preferably acousto-optic or electro-optic medium, which changes the ordinary and extraordinary refractive index.
摘要翻译：用于控制在多个光出口处在样本中激发并且/或背散射和/或反射并且包含一个或多个波长的光辐射的装置，其中将光辐射分离成不同偏振的分量出来并且激发辐射和/或检测辐射的分量通过改变普通和非凡折射率的优选双折射，优选声光或电光介质在它们的极化中受到影响。

25. 发明申请

US20090250632A1 Method and Arrangement for Collimated Microscopic Imaging 审中-公开
标题翻译：准直显微镜成像的方法和布置
公开(公告)号：US20090250632A1
公开(公告)日：2009-10-08
申请号：US12444290
申请日：2007-10-02
申请人： Michael Kempe , Gerhard Krampert , Matthias Wald , Ralf Wolleschensky
发明人： Michael Kempe , Gerhard Krampert , Matthias Wald , Ralf Wolleschensky
IPC分类号： G01N21/64
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/0032 , G02B21/0072 , G02B21/0076
摘要： A method and arrangement for collimated microscopic imaging, including a first illumination of a sample in at least one region for exciting fluorescence, and a spatially resolving detection of the sample light by detector elements, the detection being associated with the region, wherein by means of a second illumination a sub-division of the region into separate fluorescent partial regions occurs, which are associated with the detector elements. The separation of the partial regions is carried out by the spatial separation of the fluorescent regions by means of intermediate regions having reduced fluorescence or no fluorescence, and/or by means of different spectral properties of the fluorescence from the partial regions.
摘要翻译：一种用于准直显微镜成像的方法和装置，包括用于激发荧光的至少一个区域中的样品的第一照明，以及由检测器元件对样品光进行空间分辨检测，所述检测与所述区域相关联，其中通过发生与检测器元件相关联的区域的分割成分离的荧光部分区域的第二照明。部分区域的分离是通过荧光区域通过具有减少的荧光或无荧光的中间区域和/或通过来自部分区域的荧光的不同光谱性质的空间分离来进行的。

26. 发明申请

US20090159800A1 Process and Devices for Optically Sensing a Specimen with a Large Depth of Field 有权
标题翻译：用于光学感测具有大景深的样本的工艺和设备
公开(公告)号：US20090159800A1
公开(公告)日：2009-06-25
申请号：US12088610
申请日：2006-09-14
申请人： Ralf Wolleschensky
发明人： Ralf Wolleschensky
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N2021/1782 , G01N2021/1787 , G01N2201/0635 , G02B21/008
摘要： A device for optically sensing a specimen with a large depth of field has a lighting module which illuminates a zone of the specimen during a predetermined measurement period with a pattern whose phase is modified in time during the measurement period, generating a specimen light to which a corresponding time-variable phase is imparted. The device also includes a detection module having a space-resolving detection zone which records the specimen zone and has multiple recording pixels, two analysis channels which can be connected to the recording pixels, and an analysis unit is connected to both analysis channels. A control unit is provided which, during the measurement period, connects each recording pixel in synchrony with the phase of the detected specimen light to the two analysis channels, alternatively, in such a way that the detected specimen light is divided into two portions phased in relation to one another, and the analysis unit calculates an optical split-image of the specimen zone on the basis of the two phased portions supplied to the analysis channels.
摘要翻译：用于光学感测具有大景深的样本的装置具有照明模块，其在预定测量周期期间以在测量周期内的相位被修改的图案照亮样本的区域，产生样本光，其中，给予相应的时变相位。该装置还包括具有记录样本区域并具有多个记录像素的空间分辨检测区域的检测模块，可连接到记录像素的两个分析通道，并且分析单元连接到两个分析通道。提供了一种控制单元，其在测量期间将每个记录像素与检测到的样本光的相位同步地连接到两个分析通道，或者以这样一种方式将检测到的样本光分为两部分并且分析单元基于提供给分析通道的两个相位部分来计算样本区域的光学分割图像。

27. 发明授权

US07505202B2 Optical scanning microscope with line scanning 有权
标题翻译：光学扫描显微镜与线扫描
公开(公告)号：US07505202B2
公开(公告)日：2009-03-17
申请号：US11808317
申请日：2007-06-08
申请人： Ralf Wolleschensky
发明人： Ralf Wolleschensky
IPC分类号： G02B21/06
CPC分类号： G02B21/0032 , G02B21/0036
摘要： Optical scanning microscope with line scanning and with illumination of a specimen via a beam splitter, which is arranged in an objective pupil and includes at least a reflecting first portion and at least a transmitting second portion, whereby the reflecting portion serves to couple in the illumination light and the transmitting portion serves to pass the detection light in the detection direction or the transmitting portion serves to couple in the illumination light and the reflecting portion serves to couple out the detection light, with a first scanning arrangement, whereby a mechanism is provided in the detection light path for the overlay of at least one further scanning arrangement for illumination and detection.
摘要翻译：光学扫描显微镜，其具有线扫描并且经由分束器照射样本，所述分束器被布置在目标瞳孔中并且包括至少反射第一部分和至少透射第二部分，由此所述反射部分用于在照明光和透射部分用于在检测方向上通过检测光，或者透射部分用于在照明光中耦合，并且反射部分用于以第一扫描装置耦合出检测光，由此提供机构用于覆盖用于照明和检测的至少一个另外的扫描装置的检测光路。

28. 发明申请

US20090046360A1 Raster scanning light microscope with line pattern scanning and applications 审中-公开
标题翻译：光栅扫描光学显微镜与线图扫描和应用
公开(公告)号：US20090046360A1
公开(公告)日：2009-02-19
申请号：US11987218
申请日：2007-11-28
申请人： Joerg-Michael Funk , Ralf Wolleschensky , Bernhard Zimmermann , Stefan Wilhelm , Ralf Engelmann
发明人： Joerg-Michael Funk , Ralf Wolleschensky , Bernhard Zimmermann , Stefan Wilhelm , Ralf Engelmann
IPC分类号： G02B21/06
CPC分类号： G02B21/0032
摘要： Raster scanning light microscope with line pattern scanning with at least one illumination module, in which the means to achieve a variable partition of the laser light into least two illumination channels are envisioned and joint illumination of a sample takes place at the same or at different areas of the sample.
摘要翻译：具有至少一个照明模块的线阵扫描的光栅扫描光学显微镜，其中实现将激光可变分割成至少两个照明通道的装置，并且在相同或不同的区域发生样品的联合照明的样品。

29. 发明申请

US20080049294A1 INSTALLABLE PINHOLE 失效
标题翻译：安装孔
公开(公告)号：US20080049294A1
公开(公告)日：2008-02-28
申请号：US11926435
申请日：2007-10-29
申请人： KARLHEINZ BARTZKE , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
发明人： KARLHEINZ BARTZKE , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
IPC分类号： G02B26/02
CPC分类号： G02B5/005
摘要： Adjustable pinhole, particularly for the illumination beam path and/or detection beam path of a laser scanning microscope, wherein the pinhole is defined by foil edges which are adjustable relative to one another, and at least two foils, each with at least one straight edge, are advantageously arranged relative to one another and/or connected to one another in such a way that their edges describe an L-shape and the L-shaped connection pieces are arranged on one another in such a way that they define a rhombic or square light passage and they are moved relative to one another for adjusting the pinhole.
摘要翻译：特别是用于激光扫描显微镜的照明光束路径和/或检测光束路径的可调节针孔，其中针孔由相对于彼此可调节的箔边缘限定，以及至少两个箔，每个箔具有至少一个直边有利地相对于彼此布置和/或彼此连接，使得它们的边缘描述为L形，并且L形连接件以这样的方式彼此布置，使得它们限定菱形或正方形光通道并且它们相对于彼此移动以调整针孔。

30. 发明授权

US07324253B2 Installable pinhole 失效
公开(公告)号：US07324253B2
公开(公告)日：2008-01-29
申请号：US10669394
申请日：2003-09-24
申请人： Karlheinz Bartzke , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
发明人： Karlheinz Bartzke , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
IPC分类号： G02B26/02
CPC分类号： G02B5/005
摘要： Adjustable pinhole, particularly for the illumination beam path and/or detection beam path of a laser scanning microscope, wherein the pinhole is defined by foil edges which are adjustable relative to one another, and at least two foils, each with at least one straight edge, are advantageously arranged relative to one another and/or connected to one another in such a way that their edges describe an L-shape and the L-shaped connection pieces are arranged on one another in such a way that they define a rhombic or square light passage and they are moved relative to one another for adjusting the pinhole.

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