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1. 发明申请

US20080049294A1 INSTALLABLE PINHOLE 失效
标题翻译：安装孔
公开(公告)号：US20080049294A1
公开(公告)日：2008-02-28
申请号：US11926435
申请日：2007-10-29
申请人： KARLHEINZ BARTZKE , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
发明人： KARLHEINZ BARTZKE , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
IPC分类号： G02B26/02
CPC分类号： G02B5/005
摘要： Adjustable pinhole, particularly for the illumination beam path and/or detection beam path of a laser scanning microscope, wherein the pinhole is defined by foil edges which are adjustable relative to one another, and at least two foils, each with at least one straight edge, are advantageously arranged relative to one another and/or connected to one another in such a way that their edges describe an L-shape and the L-shaped connection pieces are arranged on one another in such a way that they define a rhombic or square light passage and they are moved relative to one another for adjusting the pinhole.
摘要翻译：特别是用于激光扫描显微镜的照明光束路径和/或检测光束路径的可调节针孔，其中针孔由相对于彼此可调节的箔边缘限定，以及至少两个箔，每个箔具有至少一个直边有利地相对于彼此布置和/或彼此连接，使得它们的边缘描述为L形，并且L形连接件以这样的方式彼此布置，使得它们限定菱形或正方形光通道并且它们相对于彼此移动以调整针孔。

2. 发明授权

US07324253B2 Installable pinhole 失效
公开(公告)号：US07324253B2
公开(公告)日：2008-01-29
申请号：US10669394
申请日：2003-09-24
申请人： Karlheinz Bartzke , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
发明人： Karlheinz Bartzke , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
IPC分类号： G02B26/02
CPC分类号： G02B5/005
摘要： Adjustable pinhole, particularly for the illumination beam path and/or detection beam path of a laser scanning microscope, wherein the pinhole is defined by foil edges which are adjustable relative to one another, and at least two foils, each with at least one straight edge, are advantageously arranged relative to one another and/or connected to one another in such a way that their edges describe an L-shape and the L-shaped connection pieces are arranged on one another in such a way that they define a rhombic or square light passage and they are moved relative to one another for adjusting the pinhole.

3. 发明授权

US07400433B2 Installable pinhole 失效
标题翻译：可安装针孔
公开(公告)号：US07400433B2
公开(公告)日：2008-07-15
申请号：US11926435
申请日：2007-10-29
申请人： Karlheinz Bartzke , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
发明人： Karlheinz Bartzke , Ralf Wolleschensky , Karl-Heinz Klopfleisch , Jens Hofmann
IPC分类号： G02B26/02
CPC分类号： G02B5/005
摘要： Adjustable pinhole, particularly for the illumination beam path and/or detection beam path of a laser scanning microscope, wherein the pinhole is defined by foil edges which are adjustable relative to one another, and at least two foils, each with at least one straight edge, are advantageously arranged relative to one another and/or connected to one another in such a way that their edges describe an L-shape and the L-shaped connection pieces are arranged on one another in such a way that they define a rhombic or square light passage and they are moved relative to one another for adjusting the pinhole.
摘要翻译：特别是用于激光扫描显微镜的照明光束路径和/或检测光束路径的可调节针孔，其中针孔由相对于彼此可调节的箔边缘限定，以及至少两个箔，每个箔具有至少一个直边有利地相对于彼此布置和/或彼此连接，使得它们的边缘描述为L形，并且L形连接件以这样的方式彼此布置，使得它们限定菱形或正方形光通道并且它们相对于彼此移动以调整针孔。

4. 发明申请

US20090303583A1 Device and method for the reproducible adjustment of the pin hole opening and pin hole position in laser scanning microscopes 有权
标题翻译：激光扫描显微镜中针孔开孔和针孔位置可重复调节的装置和方法
公开(公告)号：US20090303583A1
公开(公告)日：2009-12-10
申请号：US11919895
申请日：2006-04-29
申请人： Heinrich Klose , Karlheinz Bartzke , Juergen Heise , Ralf Wolleschensky , Matthias Burkhardt
发明人： Heinrich Klose , Karlheinz Bartzke , Juergen Heise , Ralf Wolleschensky , Matthias Burkhardt
IPC分类号： G02B21/06 , G01J1/20
CPC分类号： G02B21/0032 , G02B21/002
摘要： Device to adjust the position and/or size of a pinhole in a laser scanning microscope (LSM) where the pinhole is illuminated via a separate light source or the LSM laser and the pinhole is moved at a right angle to the optical axis until the receiver has the maximum intensity and the pinhole position is captured and saved together with the data attributed to the replaceable optical components.
摘要翻译：用于在激光扫描显微镜（LSM）中调整针孔的位置和/或尺寸的装置，其中针孔通过单独的光源或LSM激光器照射，并且针孔以与光轴成直角的方式移动，直到接收器具有最大强度，并且捕捉针孔位置并将其与归因于可更换光学部件的数据一起保存。

5. 发明申请

US20070081222A1 Adjustable pinhole for a laser-scanning microscope 审中-公开
标题翻译：激光扫描显微镜可调针孔
公开(公告)号：US20070081222A1
公开(公告)日：2007-04-12
申请号：US10557696
申请日：2004-05-12
申请人： Karlheinz Bartzke , Ralf Wolleschensky
发明人： Karlheinz Bartzke , Ralf Wolleschensky
IPC分类号： G02B26/02
CPC分类号： G02B21/002 , G02B5/005
摘要： An adjustable pinhole for a laser scanning microscope. The adjustable pinhole includes first and second silicon apertures movable relative to each other, each of the silicon apertures having a rectangular mirror-inverted opening, the relative movement of the openings defining a pinhole of varying size. The relative movement can be in two directions, whereby the first and second silicon apertures are displaceable with respect to one another in a first direction and whereby at least one of the silicon apertures is displaceable in a second direction perpendicular to the first direction for adjustment to obtain an exact square form for the configuration of the pinhole.
摘要翻译：激光扫描显微镜的可调针孔。可调节针孔包括可相对于彼此移动的第一和第二硅孔，每个硅孔具有矩形镜反向开口，开口的相对运动限定了不同大小的针孔。相对运动可以在两个方向上，由此第一和第二硅孔可以在第一方向上相对于彼此移位，并且由此至少一个硅孔可以在垂直于第一方向的第二方向上移位，以便调整为获得针孔配置的精确正方形。

6. 发明授权

US08759745B2 Device for the reproducible adjustment of a pin hole opening and pin hole position in laser scanning microscopes using a reflector 有权
标题翻译：用于使用反射器在激光扫描显微镜中可重复地调整针孔开口和针孔位置的装置
公开(公告)号：US08759745B2
公开(公告)日：2014-06-24
申请号：US11919895
申请日：2006-04-29
申请人： Heinrich Klose , Karlheinz Bartzke , Juergen Heise , Ralf Wolleschensky , Matthias Burkhardt
发明人： Heinrich Klose , Karlheinz Bartzke , Juergen Heise , Ralf Wolleschensky , Matthias Burkhardt
IPC分类号： G01D5/34 , H01J3/14
CPC分类号： G02B21/0032 , G02B21/002
摘要： Device to adjust the position and/or size of a pinhole in a laser scanning microscope (LSM) where the pinhole is illuminated via a separate light source or the LSM laser and the pinhole is moved at a right angle to the optical axis until the receiver has the maximum intensity and the pinhole position is captured and saved together with the data attributed to the replaceable optical components.
摘要翻译：用于在激光扫描显微镜（LSM）中调整针孔的位置和/或尺寸的装置，其中针孔通过单独的光源或LSM激光器照射，并且针孔以与光轴成直角的方式移动，直到接收器具有最大强度，并且捕捉针孔位置并将其与归因于可更换光学部件的数据一起保存。

7. 发明授权

US09588328B2 Wide-field microscope and method for wide-field microscopy 有权
标题翻译：宽视场显微镜和广视野显微镜的方法
公开(公告)号：US09588328B2
公开(公告)日：2017-03-07
申请号：US14112519
申请日：2012-03-05
申请人： Thomas Kalkbrenner , Ralf Wolleschensky , Ingo Kleppe
发明人： Thomas Kalkbrenner , Ralf Wolleschensky , Ingo Kleppe
IPC分类号： G02B21/36 , G01N21/64 , G02B21/16 , G02B27/58 , H04N5/372
CPC分类号： G02B21/365 , G01N21/6408 , G01N21/6458 , G02B21/16 , G02B21/361 , G02B21/367 , G02B27/58 , H04N5/372
摘要： A method for high-resolution PAL microscopy, wherein a sample field is imaged on a detector surface of a detector, the sample field is imaged into an image field which is smaller than the detector surface, and the image field on the detector surface is shifted, so that the same sample field is imaged in different positions located adjacent to one another on the image field in order to determine information about changes in the sample field.
摘要翻译：一种用于高分辨率PAL显微镜的方法，其中将样品场成像在检测器的检测器表面上，将样本场成像为小于检测器表面的图像场，并且检测器表面上的图像场偏移，使得相同的采样场成像在图像场上彼此相邻的不同位置，以便确定关于样本场中的变化的信息。

8. 发明申请

US20140133011A1 CONFOCAL INCIDENT-LIGHT SCANNING MICROSCOPE 有权
标题翻译：共焦事件光扫描显微镜
公开(公告)号：US20140133011A1
公开(公告)日：2014-05-15
申请号：US14130966
申请日：2012-07-05
申请人： Daniel Schwedt , Ralf Wolleschensky
发明人： Daniel Schwedt , Ralf Wolleschensky
IPC分类号： G02B21/00 , G02F1/29 , G02F1/35
CPC分类号： G02B21/0076 , G02B21/0032 , G02F1/29 , G02F1/353
摘要： A microscope which makes possible a spectrally-flexible excitation and detection of fluorescence in an economical manner. For this purpose, means for frequency conversion are arranged in the common beam path and a filter for excitation light is arranged in addition to the main beam splitter in the detection beam path. The frequency conversion achieves a spectral delimitation between illumination light, which is emitted by the light source, and excitation light which brings about fluorescence excitation in the specimen. Because the frequency conversion takes place in the common beam path after the main beam splitter, it is possible for both a spatial separation of illumination light, and excitation light and fluorescent light (detection light) emitted by the specimen, to be carried out in an economical manner at the main beamsplitter according to spectral bands because of the spectral difference between illumination light and excitation light.
摘要翻译：一种显微镜，可以以经济的方式进行光谱灵活的荧光激发和检测。为此，在公共光束路径中布置用于频率转换的装置，并且除了检测光束路径中的主分束器之外还布置有用于激发光的滤光器。频率转换实现了由光源发射的照明光与在样本中产生荧光激发的激发光之间的光谱定界。由于在主分束器之后的共同光束路径中发生频率转换，所以可以在照射光和激发光的空间分离以及由样本发射的荧光（检测光）两者中进行由于照明光和激发光之间的光谱差异，根据光谱带在主分束器处经济。

9. 发明授权

US08699130B2 Microscope, in particular laser scanning microscope 有权
标题翻译：显微镜，特别是激光扫描显微镜
公开(公告)号：US08699130B2
公开(公告)日：2014-04-15
申请号：US13255627
申请日：2010-03-06
申请人： Ralf Wolleschensky
发明人： Ralf Wolleschensky
IPC分类号： G02B21/36 , G02B21/00
CPC分类号： G02B21/0076 , G01J3/02 , G01J3/0237 , G01J3/26 , G01J3/36 , G01J3/4406 , G01J2003/1221 , G01N21/645 , G01N2021/6471 , G02B21/0064 , G02B27/1006
摘要： Microscope, particularly laser scanning microscope, for optical detection of light radiation excited in a specimen, having a detection beam path for detecting spectral components of the light radiation in a plurality of detection channels, wherein the light radiation arrives at a variable longpass filter or shortpass filter from which reflected and/or transmitted components are reflected back with a parallel offset, and the latter arrive at a detector after at least one back-reflection of this kind.
摘要翻译：显微镜，特别是激光扫描显微镜，用于在样本中激发的光辐射的光学检测，具有用于检测多个检测通道中的光辐射的光谱分量的检测光束路径，其中光辐射到达可变长通滤波器或短路反射和/或透射的分量由平行偏移反射回来的滤光器，后者在这种至少一次背反射之后到达检测器。

10. 发明申请

US20110176206A1 Microscope Having an Adjustment Device for the Focus Range 有权
标题翻译：具有调焦装置的显微镜用于对焦范围
公开(公告)号：US20110176206A1
公开(公告)日：2011-07-21
申请号：US13121886
申请日：2009-09-22
申请人： Ralf Wolleschensky , Michael Kempe
发明人： Ralf Wolleschensky , Michael Kempe
IPC分类号： G02B21/00
CPC分类号： G02B21/006 , G02B21/0068 , G02B21/241 , G02B2207/114
摘要： A microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of the light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with at least one second mirror for transmitting light from the first objective lens in the direction of the second objective lens and from the second objective lens to the detector being disposed in the optical path, which second mirror is a fully reflective mirror, or a microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with a polarizing beam splitter for splitting the object light into two components that are oriented perpendicular to each other being disposed between the first and the second objective lens for light transmission.
摘要翻译：一种具有用于调节焦距范围的装置的显微镜，包括用于在检测器的方向上传送被照射物体的物体的第一物镜，其中第二物镜沿检测器上游的光的方向设置，第二物镜之后是可以在光轴方向上调节的第一反射镜，具有至少一个第二反射镜，用于在第二物镜的方向上从第一物镜透射光并从第二物镜传播光所述检测器设置在所述光路中，所述第二反射镜是完全反射镜，或具有用于调节所述焦距的装置的显微镜，所述显微镜包括用于沿检测器的方向透射被照射物体的物体的第一物镜，其中第二物镜沿着检测器上游的光的方向设置，该第二物镜后面是第一镜像 r可以在光轴的方向上调整，其中偏振分束器用于将物体光分成两个彼此垂直取向的分量，这两个分量被布置在第一和第二物镜之间用于透光。

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