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    • 22. 发明授权
    • Vector derivatives of gluconobacter plasmid pF4
    • 葡萄糖杆菌质粒pF4的载体衍生物
    • US06399340B1
    • 2002-06-04
    • US09509565
    • 2000-06-23
    • Yoshimasa SaitoYuji NoguchiKoji YoshikawaShinsuke Soeda
    • Yoshimasa SaitoYuji NoguchiKoji YoshikawaShinsuke Soeda
    • C12P760
    • C12N9/0006C12N15/74C12P7/60
    • A DNA derived from plasmid pF4, which contains a region involved in the control of autonomous replication in a bacterial cell belonging to the genus Gluconobactor, with or without a part of the polynucleotide region not essential for the autonomous replication therein, particularly, the same DNA comprising, as the region involved in the control of autonomous replication in the bacterial cell belonging to the genus Gluconobactor, a part or the entirety of the polynucleotide of nucleotide No. 2897-3969 region of Sequence Listing SEQ:ID No. 1. A plasmid vector containing this DNA, transformant transformed with this plasmid vector, and a method for producing a physiologically active substance comprising culturing this transformant. The full length nucleotide sequence of plasmid pF4 and the region containing DNA involved in the control of automonous replication in the bacterial cell belonging to the genus Gluconobactor were specified, whereby the region not essential for the automonous replication could be removed to provide a shortened pF4. The shortening of the region in the vector for autonomous replication increases the remaining region of the vector and allows incorporation of many structural genes into the vector. Consequently, the vector can have many functions.
    • 来自质粒pF4的DNA,其含有涉及控制属于Gluconobactor属的细菌细胞中的自主复制的区域,具有或不具有对其自主复制不是必需的一部分多核苷酸区域,特别是相同的DNA 包括作为控制属于Gluconobactor属的细菌细胞中的自主复制的区域的部分或全部是序列表SEQ ID NO:1的核苷酸2897-3969区域的多核苷酸的部分或全部。质粒 含有该DNA的载体,用该质粒载体转化的转化体,以及生产生物活性物质的方法,包括培养该转化体。 规定了质粒pF4的全长核苷酸序列和涉及控制属于Gluconobactor属细菌细胞中的自动复制控制的DNA的区域,由此可以除去对于自动复制不是必需的区域以提供缩短的pF4。 自由复制载体中区域的缩短增加了载体的剩余区域,并允许将许多结构基因并入载体中。 因此,矢量可以有很多功能。
    • 24. 发明授权
    • Safety ignition means for burner installations
    • 燃烧器安装的安全点火装置
    • US4168141A
    • 1979-09-18
    • US822914
    • 1977-08-08
    • Yoshimasa Saito
    • Yoshimasa Saito
    • F23N5/22F23N5/02F23N5/20F23Q9/14F23Q9/08
    • F23Q9/14F23N5/025F23N2027/22F23N5/20
    • Safety ignition means for burner installations having a solenoid of a solenoid operated valve connected to a power source through a normally opened relay switch. The relay switch has a hold coil connected to the power source and a make coil connected to the power source through a flame switch which operates, when the flame switch is heated by the flame of the pilot burner, to energize a solenoid of a solenoid operated valve for main burner and de-energize the make coil. A normally closed bimetal switch is connected between a power supply and the safety ignition means and has a heat source connected in series with the make coil. If the pilot burner is not ignited for a predetermined period of time, the heat source heats the bimetal switch so that the bimetal switch is opened to separate the ignition means from the power supply.
    • 用于燃烧器安装的安全点火装置,其具有通过常开继电器开关连接到电源的电磁阀的螺线管。 继电器开关具有连接到电源的保持线圈和通过火焰开关连接到电源的制动线圈,当火焰开关被导向燃烧器的火焰加热时,该火焰开关操作以激励螺线管操作的螺线管 用于主燃烧器的阀,并使制动线圈断电。 常闭双金属开关连接在电源和安全点火装置之间,并具有与制线圈串联的热源。 如果引燃燃烧器没有点燃预定的时间段,则热源加热双金属开关,使得双金属开关打开以将点火装置与电源分离。