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21. 发明授权

US07148055B2 Process for production of optically active 3-hydroxypentanenitrile 失效
标题翻译：光学活性3-羟基戊腈的制备方法
公开(公告)号：US07148055B2
公开(公告)日：2006-12-12
申请号：US10491115
申请日：2002-10-03
申请人： Shigeru Kawano , Yoshihiko Yasohara
发明人： Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C12P41/00
CPC分类号： C07C253/30 , C12P13/002 , C12P13/004 , C07C255/17
摘要： The present invention provides a method for preparing optically active 3-hydroxypentanenitrile with high yield. Optically active 3-hydroxypentanenitrile is prepared by stereoselectively reducing 3-ketopentanenitrile by action of an enzyme, which asymmetrically reduces 3-ketopentanenitrile to optically active 3-hydroxypentanenitrile. Also, alkali metal salt of 3-ketopentanenitrile, which is a stable compound without problems regarding storage, can be efficiently obtained.
摘要翻译：本发明提供了一种以高产率制备光学活性3-羟基戊腈的方法。通过酶的作用立体选择性还原3-酮戊腈制备光学活性3-羟基戊腈，其将3-酮戊腈不对称地还原成光学活性3-羟基戊腈。此外，可以有效地获得作为没有储存问题的稳定化合物的3-酮戊腈的碱金属盐。

22. 发明申请

US20060046289A1 Novel carbonyl reductase, gene thereof and use of the same 有权
标题翻译：新型羰基还原酶，其基因及其用途
公开(公告)号：US20060046289A1
公开(公告)日：2006-03-02
申请号：US10512908
申请日：2003-04-30
申请人： Noriyuki Kizaki , Miho Horikawa , Yoshihiko Yasohara
发明人： Noriyuki Kizaki , Miho Horikawa , Yoshihiko Yasohara
IPC分类号： C12P7/22 , C07H21/04 , C12P21/06 , C12N9/02 , C12N1/21
CPC分类号： C12N9/0006 , C12Y101/01184
摘要： The present invention provides a novel polypeptide forming (R)-2-chloro-1-(3′-chlorophenyl)ethanol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on 2-chloro-1-(3′-chlorophenyl)ethanone with NADPH or NADH as a coenzyme, to form (R)-2-chloro-1-(31-chlorophenyl)ethanol; (2) optimum pH for activity: 5.0 to 6.0; (3) optimum temperature for activity: 40° C. to 50° C.; (4) molecular weight: about 40,000 as determined by gel filtration analysis, about 30,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
摘要翻译：本发明提供形成（R）-2-氯-1-（3'-氯苯基）乙醇的新型多肽，编码所述多肽的多核苷酸及其用途。本发明涉及具有以下物理和化学性质（1）至（4）的多肽：（1）活性：以NADPH或NADH作为辅酶作用于2-氯-1-（3'-氯苯基）乙酮，形成（R）-2-氯-1-（31-氯苯基）乙醇; （2）活性最佳pH：5.0〜6.0; （3）最适温度为40℃〜50℃。（4）分子量：约40,000，通过凝胶过滤分析测定，约30,000，通过SDS聚丙烯酰胺凝胶电泳分析测定。本发明还涉及包含序列表中SEQ ID NO：1所示氨基酸序列的多肽，编码所述多肽的多核苷酸，以及以高水平产生所述多肽的转化体。

23. 发明申请

US20050080277A1 Process for preparing optically active 2-[6-(hydroxy-methyl)-1,3-dioxan-4-yl] acetic acid derivatives 失效
标题翻译：光学活性的2- [6-（羟基 - 甲基）-1,3-二恶烷-4-基]乙酸衍生物的制备方法
公开(公告)号：US20050080277A1
公开(公告)日：2005-04-14
申请号：US10048553
申请日：2001-06-05
申请人： Akira Nishiyama , Miho Horikawa , Yoshihiko Yasohara , Noboru Ueyama , Kenji Inoue
发明人： Akira Nishiyama , Miho Horikawa , Yoshihiko Yasohara , Noboru Ueyama , Kenji Inoue
IPC分类号： C07D319/06 , C12P7/42 , C12P17/06
CPC分类号： C07D319/06 , C12P7/42 , C12P17/06
摘要： The present invention is to provide a production technology by which an optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl]acetic acid derivative, which are of value as pharmaceutical intermediates, can be produced from inexpensive and readily available starting materials without using any extraordinary equipment such as an ultra-low-temperature reactor. The present invention is a production process of an optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl]acetic acid derivative which comprises reacting an enolate, prepared by permitting a base or a 0-valent metal to act on an acetic acid ester derivative with (S)-β-hydroxy-γ-butyrolactone at a temperature not lower than −30° C. to give a dihydroxyoxohexanoic acid derivative, treating the same with an acylating agent in the presence of a base to produce a dihydroxyoxohexanoic acid monoacyl derivative, reducing this compound with a microorganism to produce a trihydroxyhexanoic acid monoacyl derivative, treating this compound with an acetal-forming reagent in the presence of an acid catalyst to produce an acyloxymethyldioxanylacetic acid derivative, and finally, subjecting this compound to solvolysis in the presence of a base.
摘要翻译：本发明提供一种生产技术，通过该技术，可以以廉价的方式生产价值为医药中间体的光学活性的2- [6-（羟甲基）-1,3-二恶烷-4-基]乙酸衍生物和容易获得的起始材料，而不使用任何非常设备，例如超低温反应器。本发明是光学活性的2- [6-（羟甲基）-1,3-二恶烷-4-基]乙酸衍生物的制备方法，该方法包括使通过使碱或0价金属在不低于-30℃的温度下用（S）-β-羟基-γ-丁内酯在乙酸酯衍生物上作用，得到二羟基氧代己酸衍生物，在酰化剂存在下用酰化剂处理碱化以制备二羟基氧己基单酰基衍生物，用微生物还原该化合物以产生三羟基己酸单酰基衍生物，在酸催化剂存在下用缩醛形成试剂处理该化合物，得到酰氧基甲基二恶烷基乙酸衍生物，最后，该化合物在碱存在下溶剂解。

24. 发明授权

US5654472A Triazole derivatives 失效
标题翻译：三唑衍生物
公开(公告)号：US5654472A
公开(公告)日：1997-08-05
申请号：US553558
申请日：1995-12-18
申请人： Yoshihiko Yasohara , Kenji Miyamoto , Noriyuki Kizaki , Shigeru Kawano , Junzo Hasegawa
发明人： Yoshihiko Yasohara , Kenji Miyamoto , Noriyuki Kizaki , Shigeru Kawano , Junzo Hasegawa
IPC分类号： C07C33/26 , C07C33/46 , C07C69/28 , C07C69/30 , C07C69/63 , C07C309/66 , C07C309/73 , C07D249/08 , C07D301/30 , C07D303/14 , C07D521/00 , C12P7/20 , C12P7/62 , C12P41/00 , C07C51/36 , C07C31/20 , C07C69/66
CPC分类号： C07D249/08 , C07C309/66 , C07C309/73 , C07C33/26 , C07C33/46 , C07C69/28 , C07C69/30 , C07C69/63 , C07D231/12 , C07D233/56 , C07D303/14 , C12P41/004 , C12P7/20 , C12P7/62
摘要： The present invention relates to a novel glycerol derivative and a process for preparing the same, and a process for preparing a triazole derivative.According to the present invention, an optical active 2-arylglycerol derivative which is a novel and useful as a synthetic intermediate of medicament can be provided and furthermore, (R)-2-(2,4-difluorophenyl)-3-(1H-1,2,4-triazole-1-yl)-propane-1,2-diol which is useful as an antifungal agent can be prepared.
摘要翻译： PCT No.PCT / JP95 / 00749 Sec。 371 1995年12月18日第 102（e）1995年12月18日日期PCT提交1995年4月17日PCT公布。出版物WO95 / 28374 日期：1995年10月26日本发明涉及新型甘油衍生物及其制备方法，以及制备三唑衍生物的方法。根据本发明，可以提供新的可用作药物合成中间体的光学活性2-芳基甘油衍生物，此外，（R）-2-（2,4-二氟苯基）-3-（1H- 1,2,4-三唑-1-基） - 丙烷-1,2-二醇，可用作抗真菌剂。

25. 发明授权

US09481871B2 Nucleic acid encoding a polpeptide having aminotransferase activity, vectors and host cells comprising the nucleic acid 有权
标题翻译：编码具有氨基转移酶活性的多肽的核酸，载体和包含该核酸的宿主细胞
公开(公告)号：US09481871B2
公开(公告)日：2016-11-01
申请号：US13876412
申请日：2011-09-28
申请人： Noriyuki Ito , Akiko Nishi , Shigeru Kawano , Yoshihiko Yasohara
发明人： Noriyuki Ito , Akiko Nishi , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C07H21/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/10 , C12P41/00 , C12P17/10
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for inexpensively and efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide exhibiting higher activity for glutamic acid as an amino donor than that for L-alanine, and, having novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译：提供了一种从酮化合物廉价有效地制备用作药物制剂，农药等的中间体的旋光性氨基化合物的方法。具体而言，对于产生具有93％以上光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，作为氨基供体的谷氨酸比L-丙氨酸具有较高活性的多肽，本发明还提供了编码该基因的基因，以及高水平表达该基因的转化体。

26. 发明授权

US09267116B2 Amino acid dehydrogenase, and process for producing L-amino acid, 2-oxo acid or D-amino acid 有权
标题翻译：氨基酸脱氢酶，以及L-氨基酸，2-氧代酸或D-氨基酸的制备方法
公开(公告)号：US09267116B2
公开(公告)日：2016-02-23
申请号：US13133444
申请日：2009-12-08
申请人： Hiroyuki Kanamaru , Ryuuji Miki , Makoto Ueda , Hironori Nanba , Yoshihiko Yasohara
发明人： Hiroyuki Kanamaru , Ryuuji Miki , Makoto Ueda , Hironori Nanba , Yoshihiko Yasohara
IPC分类号： C12N9/06 , C12N1/21 , C12P13/04 , C07H21/04 , C12P7/40 , C12P13/22 , C12P41/00 , C12N15/70 , C12N15/74
CPC分类号： C12N9/0016 , C12N9/0012 , C12N15/70 , C12N15/74 , C12P7/40 , C12P13/04 , C12P13/22 , C12P41/006
摘要： The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.
摘要翻译：本发明涉及新的氨基酸脱氢酶，编码该酶的DNA，以及引入其中的DNA的转化体。本发明还涉及生产L-氨基酸，2-氧代酸或D-氨基酸的方法，其包括使氨基酸脱氢酶或能够产生酶的微生物或转化体作用于底物化合物。氨基酸脱氢酶即使对于具有大的侧链的氨基酸或2-氧代酸，例如含芳香环的基团，该酸与常规氨基酸脱氢酶的反应性差。氨基酸脱氢酶能够廉价且高效地生产有用的光学活性氨基酸。

27. 发明授权

US09029113B2 Modified aminotransferase, gene thereof, and method for producing optically active amino compound using same 有权
标题翻译：修饰的氨基转移酶及其基因，以及使用其制备光学活性氨基化合物的方法
公开(公告)号：US09029113B2
公开(公告)日：2015-05-12
申请号：US14004293
申请日：2012-03-09
申请人： Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C12N9/10 , C12P17/12 , C12P13/00 , C12P13/04 , C12P41/00
CPC分类号： C12N9/1096 , C12P13/001 , C12P13/04 , C12P41/006
摘要： Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.
摘要翻译：提供一种从酮化合物有效地制备用作药物，农药等的中间体的光学活性氨基化合物的方法。提供：通过修饰衍生自荧光假单胞菌的氨基转移酶，与野生型酶相比，具有增加立体选择性，耐热性和耐胺性化合物的氨基转移酶活性的多肽; 编码该多肽的基因; 以及高水平表达基因的转化体。

28. 发明授权

US08664373B2 Method for producing sophorose lipid 有权
标题翻译：生产槐糖脂的方法
公开(公告)号：US08664373B2
公开(公告)日：2014-03-04
申请号：US13126532
申请日：2009-10-23
申请人： Satohiro Yanagisawa , Shigeru Kawano , Yoshihiko Yasohara
发明人： Satohiro Yanagisawa , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C07H15/04 , C07H15/10
CPC分类号： C07H15/10 , A61K8/60 , A61K31/7016 , A61Q19/00 , C07H15/04 , C11C3/04 , C12P19/44 , C12R1/72
摘要： The present invention provides a low-cost, efficient method for producing a glycolipid biosurfactant, in particular, lactonic sophorose lipids. This method is characterized by culturing a microorganism capable of producing the biosurfactant under limited oxygen supply. The present invention enables preferential production of lactonic sophorose lipids and facilitates recovery of the lactonic sophorose lipids in a solid form. Further, the present invention enables production of high purity acidic sophorose lipids by hydrolyzing high purity lactonic sophorose lipids produced by the above method. The present invention also provides lactonic sophorose lipids that possess strong antibacterial and antifungal activities, and an antibacterial and/or antifungal agent containing the sophorose lipids.
摘要翻译：本发明提供了制备糖脂生物表面活性剂，特别是内酯槐糖脂的低成本，有效的方法。该方法的特征在于培养能够在有限供氧下产生生物表面活性剂的微生物。本发明能够优先生产内酯槐糖脂，并有利于以固体形式回收内酯槐糖脂。此外，本发明能够通过水解由上述方法生产的高纯度内酯槐糖脂来生产高纯度酸性槐糖脂。本发明还提供具有强抗菌和抗真菌活性的内酯槐糖脂，以及含有槐糖脂的抗菌和/或抗真菌剂。

29. 发明申请

US20130210092A1 Novel Transaminase, Gene Encoding the Same, and Method Using Them 有权
标题翻译：新型转氨酶，编码基因的方法和使用它们的方法
公开(公告)号：US20130210092A1
公开(公告)日：2013-08-15
申请号：US13876400
申请日：2011-09-28
申请人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C12N9/10
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide having high resistance to a water-soluble organic solvent and novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译：提供了从酮化合物有效地制备用作药物制剂，农药等的中间体的光学活性氨基化合物的方法。具体地说，对水溶性有机溶剂具有高抗性的多肽和具有93％以上的光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，编码该多肽的基因，以及转化体本文还提供了表达高水平的基因。

30. 发明授权

US08304216B2 Method for production of erythro-or threo-2-amino-3-hydroxypropionic acid ester, novel carbonyl reductase, gene for the reductase, vector, transformant, and method for production of optically active alcohol using those 有权
标题翻译：用于生产红血球或苏氨酸-2-氨基-3-羟基丙酸酯的方法，新的羰基还原酶，还原酶的基因，载体，转化体和使用这些的生产光学活性醇的方法
公开(公告)号：US08304216B2
公开(公告)日：2012-11-06
申请号：US12225683
申请日：2007-03-29
申请人： Tozo Nishiyama , Yoshihiko Yasohara
发明人： Tozo Nishiyama , Yoshihiko Yasohara
IPC分类号： C12P7/02 , C12P7/62 , C07H21/04
CPC分类号： C12P13/04 , C12N9/0006
摘要： The present invention has its object to provide a method of producing an erythro- or threo-2-amino-3-hydroxypropionic acid ester, and so forth. The present invention relates to: a method of asymmetrically reducing an N-2-amino-3-oxopropionic acid ester by allowing cells of a microorganism to act thereon; a polypeptide having an activity of asymmetrically reducing a carbonyl compound to give an optically active alcohol, which is isolated from a microorganism belonging to genus Brevundimonas; a DNA coding for the polypeptide; and a transformant producing the polypeptide. The invention also relates to a method of producing an optically active alcohol by reducing a carbonyl compound with the help of the polypeptide or the transformant.
摘要翻译：本发明的目的是提供一种制备赤式或苏氨酸-2-氨基-3-羟基丙酸酯的方法等。本发明涉及：通过使微生物的细胞在其上作用而不对称地还原N-2-氨基-3-氧代丙酸酯的方法; 具有不对称还原羰基化合物的活性的多肽，得到从Brevundimonas属的微生物分离的光学活性醇; 编码该多肽的DNA; 和产生多肽的转化体。本发明还涉及通过在多肽或转化体的帮助下还原羰基化合物来制备光学活性醇的方法。

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