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21. 发明申请

US20080081340A1 Enzymatic and chemical method for increased peptide detection sensitivity using surface enhanced raman scattering (SERS) 审中-公开
标题翻译：使用表面增强拉曼散射（SERS）增加肽检测灵敏度的酶和化学方法
公开(公告)号：US20080081340A1
公开(公告)日：2008-04-03
申请号：US11529574
申请日：2006-09-29
申请人： Anil Patwardhan , Handong Li , Narayan Sundararajan
发明人： Anil Patwardhan , Handong Li , Narayan Sundararajan
IPC分类号： G01N33/53 , G01N33/553 , C12P21/06 , C12M3/00
CPC分类号： G01N33/68 , C12P21/06 , G01N33/54373
摘要： The sensitivity of surface enhanced Raman spectroscopy to silver nano-particle/peptide aggregates is increased by prior treatment of the peptides. According to a first type of embodiment, an enzyme such as Glu-C is used for protein(s) digestion based on the enzyme's ability to cleave proteins at a selected location having a negative charge, such as at aspartic acid and glutamic acid. This type of digestion is used to derive a higher proportion of positively charged component peptides sequences as compared to the component peptides sequences obtained by standard tryptic digestion of protein(s). According to a second type of embodiment, methyl-esterification of peptides suppresses the negative charge contributions of portions of the peptides such as aspartic acid, glutamic acid, and the C-terminus. Both types of embodiments result in increased binding affinity of the resulting component sequence peptides with negatively charged nano-particles such as silver nano-particles. According to yet other embodiments, the first and second types of embodiments can be combined for further sensitivity increase.
摘要翻译：表面增强拉曼光谱对银纳米颗粒/肽聚集体的敏感性通过先前处理肽而增加。根据第一类型的实施方案，基于酶在具有负电荷的选定位置（例如天冬氨酸和谷氨酸）切割蛋白质的能力，使用诸如Glu-C的酶进行蛋白质消化。与通过蛋白质的标准胰蛋白酶消化获得的成分肽序列相比，这种类型的消化用于导出更高比例的带正电荷的组分肽序列。根据第二类型的实施方案，肽的甲基酯化抑制了肽部分的负电荷贡献，例如天冬氨酸，谷氨酸和C末端。两种类型的实施方案导致所得组分序列肽与带负电荷的纳米颗粒如银纳米颗粒的结合亲和力增加。根据其他实施例，可以组合第一和第二类型的实施例以进一步提高灵敏度。

22. 发明申请

US20050003496A1 Nucleic acid labeling compounds 有权
标题翻译：核酸标记化合物
公开(公告)号：US20050003496A1
公开(公告)日：2005-01-06
申请号：US10745916
申请日：2003-12-23
申请人： Glenn McGall , Anthony Barone , Handong Li
发明人： Glenn McGall , Anthony Barone , Handong Li
IPC分类号： C12P17/00 , C07
CPC分类号： C12P17/00
摘要： Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. Methods for making such heterocyclic compounds are also disclosed. The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.
摘要翻译：公开了含有杂环衍生物的核酸标记化合物。还公开了制备这种杂环化合物的方法。标记化合物适合于在末端或内部酶促附着于核酸，以提供核酸检测机制。

23. 发明授权

US09551682B2 High throughput biochemical detection using single molecule fingerprinting arrays 有权
标题翻译：使用单分子指纹图谱的高通量生物化学检测
公开(公告)号：US09551682B2
公开(公告)日：2017-01-24
申请号：US13538346
申请日：2012-06-29
申请人： Oguz H. Elibol , Grace M. Credo , Xing Su , Madoo Varma , Jonathan S. Daniels , Drew Hall , Handong Li , Noureddine Tayebi , Kai Wu
发明人： Oguz H. Elibol , Grace M. Credo , Xing Su , Madoo Varma , Jonathan S. Daniels , Drew Hall , Handong Li , Noureddine Tayebi , Kai Wu
IPC分类号： G01N27/327 , B82Y15/00 , C40B20/04 , C40B50/16
CPC分类号： G01N27/3277 , B01J19/0046 , B01J2219/00608 , B01J2219/00653 , B01J2219/00722 , B81B7/04 , B81B2201/0214 , B81B2201/058 , B82Y15/00 , C40B20/04 , C40B50/16 , G01N27/3278 , G01N27/4145 , G01N27/4146 , G01N27/4148
摘要： Various embodiments provide devices, methods, and systems for high throughput biomolecule detection using transducer arrays. In one embodiment, a transducer array made up of transducer elements may be used to detect byproducts from chemical reactions that involve redox genic tags. Each transducer element may include at least a reaction chamber and a fingerprinting region, configured to flow a fluid from the reaction chamber through the fingerprinting region. The reaction chamber can include a molecule attachment region and the fingerprinting region can include at least one set of electrodes separated by a nanogap for conducting redox cycling reactions. In embodiments, by flowing the chamber content obtained from a reaction of a latent redox tagged probe molecule, a catalyst, and a target molecule in the reaction chamber through the fingerprinting region, the redox cycling reactions can be detected to identify redox-tagged biomolecules.
摘要翻译：各种实施方案提供使用换能器阵列进行高通量生物分子检测的装置，方法和系统。在一个实施例中，由换能器元件组成的换能器阵列可用于检测涉及氧化还原基因标签的化学反应的副产物。每个换能器元件可以至少包括反应室和指纹区域，其被配置为使来自反应室的流体通过指纹区域流动。反应室可以包括分子附着区域，并且指纹区域可以包括由纳米间隙分开的至少一组电极，用于进行氧化还原循环反应。在实施方案中，通过使反应室中潜氧氧化还原标记的探针分子，催化剂和靶分子的反应通过指纹区域流动所述腔室内容物，可以检测氧化还原循环反应以鉴定氧化还原标记的生物分子。

24. 发明授权

US08945509B2 Magnetic beads having surface glycoconjugates and use thereof 有权
标题翻译：具有表面糖缀合物的磁珠及其用途
公开(公告)号：US08945509B2
公开(公告)日：2015-02-03
申请号：US13215157
申请日：2011-08-22
申请人： Aldrich Lau , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
发明人： Aldrich Lau , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
IPC分类号： A61K51/00 , A61B5/055 , C12Q1/70
CPC分类号： G01N33/54326 , C12N1/02 , C12N13/00 , C12Q1/689 , G01N33/54353 , G01N33/56911
摘要： Magnetic beads that include polyvalent ligands comprising various carbohydrates are described. Methods for fabricating such magnetic beads are also provided as well as methods of their use to capture and enrich pathogen cell population for subsequent culture, lysis and identification.
摘要翻译：描述包括包含各种碳水化合物的多价配体的磁珠。还提供了制备这种磁珠的方法以及它们用于捕获和富集病原体细胞群以用于随后的培养，裂解和鉴定的方法。

25. 发明授权

US08338097B2 Method and apparatus for combined electrochemical synthesis and detection of analytes 有权
标题翻译：用于组合电化学合成和分析物检测的方法和装置
公开(公告)号：US08338097B2
公开(公告)日：2012-12-25
申请号：US13046895
申请日：2011-03-14
申请人： Hernan Castro , Gordon Holt , Brandon Barnett , Handong Li , Narayan Sundararajan , Wei Wang
发明人： Hernan Castro , Gordon Holt , Brandon Barnett , Handong Li , Narayan Sundararajan , Wei Wang
IPC分类号： C12Q1/68 , G01N33/53 , G01N15/06 , G01N27/00 , G01N33/566 , C12M1/00 , C12M1/34 , C07H21/02
CPC分类号： C25B3/00 , G01N27/226 , H01M14/00
摘要： Described are devices and methods for detecting binding on an electrode surface. In addition, devices and methods for electrochemically synthesizing polymers and devices and methods for synthesizing and detecting binding to the polymer on a common integrated device surface are described.
摘要翻译：描述了用于检测电极表面上的结合的装置和方法。此外，描述了用于电化学合成聚合物的装置和方法以及用于合成和检测在共同的集成器件表面上与聚合物结合的方法。

26. 发明申请

US20110159305A1 Intermediates And Methods For Forming Passivated Surfaces On Oxide Layers And Articles Produced Thereby 审中-公开
标题翻译：中间体和方法在氧化物层和由此产生的制品上形成钝化表面
公开(公告)号：US20110159305A1
公开(公告)日：2011-06-30
申请号：US13044759
申请日：2011-03-10
申请人： Steven M. MENCHEN , Christina E. Inman , Meng Taing , Khai Luong , Handong Li
发明人： Steven M. MENCHEN , Christina E. Inman , Meng Taing , Khai Luong , Handong Li
IPC分类号： B32B15/00
CPC分类号： G01N33/54393 , G01N33/551 , Y10T428/31678
摘要： Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.
摘要翻译：描述了用于在氧化物层上形成钝化表面的中间体和方法以及由此制备的制品。氧化物表面上的羟基或氢氧基与式Y（L-Pol）m的金属试剂反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol “表示钝化剂，例如聚乙二醇，烃或碳氟化合物。所得到的改性表面可以进一步与具有磷酸酯官能团的钝化剂或包含钝化部分和多个与Y形成复合物的多个官能团的多价试剂反应。钝化剂还可以包括官能团例如生物素，以提供具有所需功能的表面。被钝化的表面表现出与生物分子的最小结合，并可用于单分子检测方案。

27. 发明申请

US20090142772A1 Devices and Methods for the Detection of Analytes 有权
标题翻译：用于检测分析物的装置和方法
公开(公告)号：US20090142772A1
公开(公告)日：2009-06-04
申请号：US12167292
申请日：2008-07-03
申请人： Aldrich N.K. Lau , Robert G. Eason , Kristian M. Scaboo , Handong Li
发明人： Aldrich N.K. Lau , Robert G. Eason , Kristian M. Scaboo , Handong Li
IPC分类号： G01N33/53 , C12M1/34
CPC分类号： G01N33/54366 , B01L3/5027 , B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/069 , B01L2300/0816 , B01L2300/0864 , G01N33/54326 , G01N2400/00
摘要： System and methods for detecting analytes such as pathogenic cells are described. The methods allow for the direct measurement of analytes such as pathogenic organisms without the need for sample preparation and/or PCR. The devices can be used individually as point-of-use sensors for airborne pathogens and other pathogenic organisms in foods and agriculture products.
摘要翻译：描述了用于检测分析物如致病细胞的系统和方法。这些方法允许直接测量分析物如病原体，而不需要样品制备和/或PCR。这些装置可以单独用作食品和农产品中的空气传播病原体和其他致病生物的使用点传感器。

28. 发明申请

US20080160635A1 Method and apparatus for combined electrochemical synthesis and detection of analytes 有权
标题翻译：用于组合电化学合成和分析物检测的方法和装置
公开(公告)号：US20080160635A1
公开(公告)日：2008-07-03
申请号：US11646602
申请日：2006-12-28
申请人： Hernan Adolfo Castro , Gordon Holt , Brandon Barnett , Handong Li , Narayan Sundararajan , Wei Wang
发明人： Hernan Adolfo Castro , Gordon Holt , Brandon Barnett , Handong Li , Narayan Sundararajan , Wei Wang
IPC分类号： G01N33/556
CPC分类号： C25B3/00 , G01N27/226 , H01M14/00
摘要： Described are devices and methods for detecting binding on an electrode surface. In addition, devices and methods for electrochemically synthesizing polymers and devices and methods for synthesizing and detecting binding to the polymer on a common integrated device surface are described.
摘要翻译：描述了用于检测电极表面上的结合的装置和方法。此外，描述了用于电化学合成聚合物的装置和方法以及用于合成和检测在共同的集成器件表面上与聚合物结合的方法。

29. 发明申请

US20070154894A1 Labeling and non-enzymatic fragmentation of cDNA using a ribonucleoside triphosphate analog 审中-公开
标题翻译：使用核糖核苷三磷酸酯类似物进行cDNA的标记和非酶切片段化
公开(公告)号：US20070154894A1
公开(公告)日：2007-07-05
申请号：US11323068
申请日：2005-12-30
申请人： Glenn McGall , Handong Li , Anthony Barone
发明人： Glenn McGall , Handong Li , Anthony Barone
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12N15/1096 , C12Q1/6806 , C12Q2523/107
摘要： In accordance with the present invention, methods are presented for labeling a cDNA strand with a labeled ribonucleotide base precursor which upon exposure to Mg2+, heat and base cleaves the cDNA at each place of incorporation of an RNA. In accordance with an aspect of the present invention, compounds selected from the group consisting of are incorporated into the growing strand of a cDNA by a reverse transcriptase or a mutant reverse transcriptase. After subject the strands to Mg2+, base and heat, the 3′ OH causes cleavage of the cDNA leaving a 2′OH phosphate with a biotin label. The biotin provides a label which may be bound to streptavidin and thereafter hybridized to a nucleic acid array.
摘要翻译：根据本发明，提供了用标记的核糖核苷酸碱基前体标记cDNA链的方法，其在暴露于Mg 2+时，加热和碱基在每个引入RNA的位置切割cDNA。根据本发明的一个方面，选自组合的化合物通过逆转录酶或突变逆转录酶掺入cDNA的生长链中。在将基因经过碱基和加热后，3'OH引起用生物素标记物离开2'OH磷酸酯的cDNA的切割。生物素提供可以与链霉抗生物素蛋白结合的标记，然后与核酸阵列杂交。

30. 发明申请

US20060147965A1 Label free analysis of nucleic acids 有权
标题翻译：无标签分析核酸
公开(公告)号：US20060147965A1
公开(公告)日：2006-07-06
申请号：US11313874
申请日：2005-12-20
申请人： Andrea Cuppoletti , Handong Li
发明人： Andrea Cuppoletti , Handong Li
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6834 , C12Q2565/107 , C12Q2563/107
摘要： A method is provided for label free analysis of nucleic acid materials. In accordance with the present invention, a fluorescent material is provided having a certain fluorescence emission without nucleic acids attached thereto. The fluorescent material of the present invention, has a greater emission upon the binding of a single stranded nucleic acid and an even greater emission upon the binding of a double stranded nucleic acid allowing detection of double stranded binding without using a label attached to the DNA.
摘要翻译：提供了一种用于无标记分析核酸材料的方法。根据本发明，提供具有一定荧光发射的荧光材料，而不附加核酸。本发明的荧光材料在单链核酸结合时具有更大的发射，并且在双链核酸的结合时具有甚至更大的发射，从而允许检测双链结合而不使用与DNA连接的标记。

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