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1. 发明申请

US20050003496A1 Nucleic acid labeling compounds 有权
标题翻译：核酸标记化合物
公开(公告)号：US20050003496A1
公开(公告)日：2005-01-06
申请号：US10745916
申请日：2003-12-23
申请人： Glenn McGall , Anthony Barone , Handong Li
发明人： Glenn McGall , Anthony Barone , Handong Li
IPC分类号： C12P17/00 , C07
CPC分类号： C12P17/00
摘要： Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. Methods for making such heterocyclic compounds are also disclosed. The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.
摘要翻译：公开了含有杂环衍生物的核酸标记化合物。还公开了制备这种杂环化合物的方法。标记化合物适合于在末端或内部酶促附着于核酸，以提供核酸检测机制。

2. 发明申请

US20070154894A1 Labeling and non-enzymatic fragmentation of cDNA using a ribonucleoside triphosphate analog 审中-公开
标题翻译：使用核糖核苷三磷酸酯类似物进行cDNA的标记和非酶切片段化
公开(公告)号：US20070154894A1
公开(公告)日：2007-07-05
申请号：US11323068
申请日：2005-12-30
申请人： Glenn McGall , Handong Li , Anthony Barone
发明人： Glenn McGall , Handong Li , Anthony Barone
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12N15/1096 , C12Q1/6806 , C12Q2523/107
摘要： In accordance with the present invention, methods are presented for labeling a cDNA strand with a labeled ribonucleotide base precursor which upon exposure to Mg2+, heat and base cleaves the cDNA at each place of incorporation of an RNA. In accordance with an aspect of the present invention, compounds selected from the group consisting of are incorporated into the growing strand of a cDNA by a reverse transcriptase or a mutant reverse transcriptase. After subject the strands to Mg2+, base and heat, the 3′ OH causes cleavage of the cDNA leaving a 2′OH phosphate with a biotin label. The biotin provides a label which may be bound to streptavidin and thereafter hybridized to a nucleic acid array.
摘要翻译：根据本发明，提供了用标记的核糖核苷酸碱基前体标记cDNA链的方法，其在暴露于Mg 2+时，加热和碱基在每个引入RNA的位置切割cDNA。根据本发明的一个方面，选自组合的化合物通过逆转录酶或突变逆转录酶掺入cDNA的生长链中。在将基因经过碱基和加热后，3'OH引起用生物素标记物离开2'OH磷酸酯的cDNA的切割。生物素提供可以与链霉抗生物素蛋白结合的标记，然后与核酸阵列杂交。

3. 发明申请

US20060147963A1 Detection of polynucleotides on nucleic acid arrays using azido-modified triphosphate nucleotide analogs 审中-公开
标题翻译：使用叠氮基修饰的三磷酸核苷酸类似物检测核酸阵列上的多核苷酸
公开(公告)号：US20060147963A1
公开(公告)日：2006-07-06
申请号：US11313480
申请日：2005-12-20
申请人： Anthony Barone , Handong Li , Glenn McGall
发明人： Anthony Barone , Handong Li , Glenn McGall
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12N15/1096 , C12Q1/6806 , C12Q1/6837 , C12Q2600/158 , C12Q2525/113 , C12Q2565/501 , C12Q2521/107 , C12Q2525/143 , C12Q2525/117
摘要： Methods are provided for detecting hybridization of a polynucleotide to a nucleic acid array by chemically modifying the polynucleotide to contain a detectable label. According to one aspect of the present invention, a method is provided for detecting the presence of a mRNA in a nucleic acid sample, the method having the steps of providing a mRNA sample and azido modified nucleotides, hybridizing a primer to the mRNA, reversed transcribing the mRNA to provide azido modified DNA, followed by reacting the azido groups with a detectable label, hybridizing the labeled RNA to a nucleic acid array and detecting the presence of the mRNA. Still other methods are provided for detecting the presence or absence of a polynucleotide of interest on a nucleic acid array, the method having the steps of providing a nucleic acid sample comprising a polynucleotide; providing an enzyme to amplify the polynucleotide using an azido nucleotide derivative; amplifying said polynucleotide to provide azido labeled amplified nucleic acids; reacting the azido groups on said nucleic acids with a detectable label to provide labeled nucleic acids; hybridizing said amplified nucleic acids to a nucleic acid array; and detecting the presence or absence of said polynucleotide. Still other methods are presented for detecting polynucleotides on a nucleic acid array using ligases and terminal transferases to end label polynucleotides.
摘要翻译：提供了通过化学修饰多核苷酸以含有可检测标记物来检测多核苷酸与核酸阵列的杂交的方法。根据本发明的一个方面，提供了一种用于检测核酸样品中mRNA的存在的方法，该方法具有以下步骤：提供mRNA样品和叠氮基修饰的核苷酸，将引物与mRNA杂交，逆转录 mRNA以提供叠氮基修饰的DNA，然后使叠氮基与可检测标记反应，将标记的RNA与核酸阵列杂交并检测mRNA的存在。还提供了用于在核酸阵列上检测感兴趣的多核苷酸的存在或不存在的其它方法，所述方法具有提供包含多核苷酸的核酸样品的步骤; 提供使用叠氮基核苷酸衍生物扩增多核苷酸的酶; 扩增所述多核苷酸以提供叠氮基标记的扩增核酸; 使所述核酸上的叠氮基与可检测标记物反应以提供标记的核酸; 将所述扩增的核酸与核酸阵列杂交; 并检测所述多核苷酸的存在或不存在。还提出了使用连接酶和末端转移酶终止标记多核苷酸检测核酸阵列上的多核苷酸的其它方法。

4. 发明申请

US20060257560A1 Polymer surfaces for insitu synthesis of polymer arrays 审中-公开
标题翻译：用于聚合物阵列的本体合成的聚合物表面
公开(公告)号：US20060257560A1
公开(公告)日：2006-11-16
申请号：US11317509
申请日：2006-07-13
申请人： Anthony Barone , Handong Li , Glenn McGall
发明人： Anthony Barone , Handong Li , Glenn McGall
IPC分类号： C08F2/46 , B05D3/12 , B05D1/18
CPC分类号： C03C17/001 , C03C17/32 , C03C17/328 , C03C2217/76 , C08F2/46 , C08L39/06 , C08L2205/02 , C09D139/06 , C08L2666/06
摘要： In one aspect of the present invention polymers are used to create films providing three-dimensional array substrates. The films were stable and presented good hydroxyl group numbers as compared with arrays without polymer films. It is an object of the present invention that three dimensional arrays substrates provide a means to obtain higher density polymer arrays.
摘要翻译：在本发明的一个方面，使用聚合物来制备提供三维阵列基底的薄膜。与没有聚合物膜的阵列相比，膜是稳定的并呈现出良好的羟基数。本发明的目的是三维阵列基底提供获得更高密度聚合物阵列的手段。

5. 发明授权

US06773888B2 Photoactivatable silane compounds and methods for their synthesis and use 失效
标题翻译：可光活化硅烷化合物及其合成和使用方法
公开(公告)号：US06773888B2
公开(公告)日：2004-08-10
申请号：US10192598
申请日：2002-07-09
申请人： Handong Li , Glenn McGall
发明人： Handong Li , Glenn McGall
IPC分类号： C12Q168
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00378 , B01J2219/00608 , B01J2219/00612 , B01J2219/00628 , B01J2219/00637 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C40B50/18 , C40B60/14 , G03F7/165 , Y10T428/31663
摘要： In one aspect of the invention, photoactivatable silane compounds and methods for their synthesis and use are provided. In one embodiment, the photoactivatable silane compounds synthesized are represented by a formula: PG-LS-SN, wherein PG is a photoactivatable group, LS is a linkage and spacer group, and SN is a silane group. The silanes allow the photoactivatable silane compounds to be covalently bound to the surface of a substrate such as silica. The photoactivatable group forms a hydrophobic layer that can be photochemically cross-linked with a layer of hydrophilic functional polymers. In another embodiment, a method is disclosed to synthesize a substrate of hydrophobic layers and hydrophilic functional polymer layers thereafter onto glass surfaces. An array of biopolymers such as nucleic acids and peptides may then be covalently attached to the substrate using photolithography and biopolymer synthesis.
摘要翻译：在本发明的一个方面，提供了可光活化的硅烷化合物及其合成和使用的方法。在一个实施方案中，合成的可光活化硅烷化合物由下式表示：PG-LS-SN，其中PG是可光活化基团，LS是连接基和间隔基，SN是硅烷基。硅烷使可光活化的硅烷化合物共价结合到诸如二氧化硅的底物表面。可光活化基团形成疏水层，其可以与一层亲水性官能聚合物光化学交联。在另一个实施方案中，公开了合成疏水层的底物和亲水性功能聚合物层之后的玻璃表面的方法。然后使用光刻和生物聚合物合成将一系列生物聚合物如核酸和肽共价连接到基底上。

6. 发明申请

US20060147985A1 Methods and compositions for monitoring polymer array synthesis 审中-公开
标题翻译：监测聚合物阵列合成的方法和组成
公开(公告)号：US20060147985A1
公开(公告)日：2006-07-06
申请号：US11369628
申请日：2006-03-07
申请人： Anthony Barone , Glenn McGall , Evelyn Chai , Nam Ngo
发明人： Anthony Barone , Glenn McGall , Evelyn Chai , Nam Ngo
IPC分类号： C40B40/08 , C12Q1/68 , C07H21/04
CPC分类号： G01N33/54366 , C07H21/04 , C12Q1/6837 , C12Q2565/137 , C12Q2545/114 , C12Q2523/107
摘要： VLSIPS™ manufacturing processes are of increasing commercial importance. The present invention provides methods and compositions for monitoring the efficiency and quality of polymer synthesis in VLSIPS™ arrays. Methods for monitoring polymer synthesis in an array on a substrate are provided. Mono-isomeric labels for the labeling of synthetic polymer arrays are provided. Methods and compositions for post-synthetically labeling polymers in polymer arrays are also provided.
摘要翻译： VLSIPS（TM）制造工艺在商业上的重要性日益增加。本发明提供了用于监测VLSIPS TM阵列中聚合物合成的效率和质量的方法和组合物。提供了在基板上阵列中监测聚合物合成的方法。提供了用于标记合成聚合物阵列的单异构体标记。还提供了聚合物阵列中后合成标记聚合物的方法和组合物。

7. 发明申请

US20050287563A1 Nucleic acid labeling compounds 有权
公开(公告)号：US20050287563A1
公开(公告)日：2005-12-29
申请号：US11125338
申请日：2005-05-10
申请人： Glenn McGall , Anthony Barone
发明人： Glenn McGall , Anthony Barone
IPC分类号： A61K49/00 , C07H21/04 , C12Q1/68
CPC分类号： A61K49/0043 , A61K49/0032 , A61K49/0052 , C07H21/04
摘要： Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. The heterocyclic derivative containing compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.

8. 发明申请

US20050191646A1 Nucleic acid analysis techniques 审中-公开
标题翻译：核酸分析技术
公开(公告)号：US20050191646A1
公开(公告)日：2005-09-01
申请号：US10961341
申请日：2004-10-07
申请人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
发明人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
IPC分类号： C07B61/00 , C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/00 , C12Q1/68
CPC分类号： C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6809 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , G16B25/00 , G16B30/00 , C12Q2525/161 , C12Q2565/501 , C12Q2561/125
摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.
摘要翻译：本发明提供用于鉴定两个或多个样品之间的核酸丰度差异（例如，表达水平）的简化方法。所述方法包括提供包含大量（例如大于1,000个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置是已知的。来自两个或更多个样品的核酸样品（例如mRNA）与探针阵列杂交，并检测杂交模式。样本之间的杂交模式的差异表明这些样品之间各种基因的表达差异。本发明还提供了一种终止标记核酸的方法。在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶连接到核酸上。因此，例如，当核酸是RNA时，可以使用RNA连接酶连接标记的寡核糖核苷酸。在另一个实施方案中，末端标记可以通过提供核酸，提供标记的核苷三磷酸和使用末端转移酶将核苷三磷酸与核酸连接来实现。

9. 发明申请

US20050101765A1 Photocleavable protecting groups 审中-公开
标题翻译：可透光保护组
公开(公告)号：US20050101765A1
公开(公告)日：2005-05-12
申请号：US11016380
申请日：2004-12-17
申请人： Anthony Barone , Glenn McGall
发明人： Anthony Barone , Glenn McGall
IPC分类号： C07B61/00 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K1/04 , C07K1/06 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C07K1/02 , C07C271/10
CPC分类号： B82Y30/00 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , C07B2200/11 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K1/04 , C07K1/047 , C07K1/062 , C07K1/063 , C07K1/064 , C07K1/065 , C07K1/066 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , Y02P20/55
摘要： Novel compounds are provided, which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Y, wherein Y is a chemical structure as shown in FIG. 1. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.
摘要翻译：提供新的化合物，其可用作化学合成中的连接基团，优选在寡核苷酸和多肽的固相合成中。这些化合物通常是光不稳定的并且包括可以通过光解去除以揭示反应性基团的保护基团。保护基具有通式Y，其中Y是如图所示的化学结构。 1 。还提供了使用上述保护的化合物从成分分子形成多个化合物在载体上的方法，每种化合物占据支持体的单独的预定区域。

10. 发明申请

US20050123956A1 Methods for modifying DNA for microarray analysis 审中-公开
标题翻译：修改DNA用于微阵列分析的方法
公开(公告)号：US20050123956A1
公开(公告)日：2005-06-09
申请号：US10951983
申请日：2004-09-27
申请人： John Blume , Yanxiang Cao , Glenn McGall , Kyle Cole , Fredrick Christians , Kai Wu , Linda Hsie , Charles Miyada , Anthony Barone , Vivi Truong
发明人： John Blume , Yanxiang Cao , Glenn McGall , Kyle Cole , Fredrick Christians , Kai Wu , Linda Hsie , Charles Miyada , Anthony Barone , Vivi Truong
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/683 , C12Q2565/501 , C12Q2525/119 , C12Q2521/531
摘要： In one aspect of the invention, methods and compositions are provided for fragmenting nucleic acid samples. Fragmented nucleic acid samples may be used for hybridization with microarrays.
摘要翻译：在本发明的一个方面，提供了用于片段化核酸样品的方法和组合物。分离的核酸样品可用于与微阵列杂交。

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