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21. 发明申请

US20090215188A1 Method for labeling or treating a biological sample containing biological molecules of interest, in particular nucleic acids 有权
标题翻译：用于标记或处理含有感兴趣的生物分子，特别是核酸的生物样品的方法
公开(公告)号：US20090215188A1
公开(公告)日：2009-08-27
申请号：US11919668
申请日：2006-05-31
申请人： Eloy Bernal-Mendez , Ali Laayoun , Lionel Menou
发明人： Eloy Bernal-Mendez , Ali Laayoun , Lionel Menou
IPC分类号： G01N33/48 , C07H1/00
CPC分类号： G01N33/58 , G01N33/532 , G01N33/54353 , Y10T436/11 , Y10T436/143333
摘要： The present invention relates to a method for labeling biological molecules of interest contained in a biological sample, consisting in: a) providing a reaction vessel, b) immobilizing capture molecules, which are capable of binding a label of the biological molecules of interest, on a solid support of the vessel, c) introducing the biological sample but also at least one label of the biological molecules of interest into said vessel and optionally any ingredient required for labeling or prelabeling the molecules of interest, d) incubating the vessel content and immobilizing the label which is not reacted with the molecules of interest by binding to the capture molecules, and e) using the labeled molecules of interest for subsequent steps. A method for treating a biological sample is also disclosed.Said invention is preferably used in a manual or automated method for purifying nucleic acids.
摘要翻译：本发明涉及用于标记生物样品中所含感兴趣生物分子的方法，其包括：a）提供反应容器，b）使能够将感兴趣的生物分子的标记结合的捕获分子固定在所述容器的固体支持物，c）将所述生物学样品引入所述生物样品，还将所述生物分子的至少一个标记物引入所述容器，以及任选的任何标记或预标记目的分子所需的成分，d）孵育所述容器内容物并固定通过结合捕获分子不与感兴趣的分子反应的标记，和e）使用目的标记的分子进行后续步骤。还公开了一种用于处理生物样品的方法。所述发明优选以用于纯化核酸的手动或自动化方法使用。

22. 发明授权

US06902891B2 Process for labeling a nucleic acid 有权
标题翻译：核酸标记方法
公开(公告)号：US06902891B2
公开(公告)日：2005-06-07
申请号：US09736151
申请日：2000-12-15
申请人： Ali Laayoun , Lionel Menou , Christelle Tora , Aloke R. Banerjee , Michael M. Becker , Kenneth A. Browne , Matthew C. Friedenberg , Fred F. Hajjar
发明人： Ali Laayoun , Lionel Menou , Christelle Tora , Aloke R. Banerjee , Michael M. Becker , Kenneth A. Browne , Matthew C. Friedenberg , Fred F. Hajjar
IPC分类号： C07H21/00 , C12Q1/68 , C07D225/00 , C07D259/00 , C07H21/04 , C12P19/34
CPC分类号： C07H21/00 , C12Q2523/107
摘要： A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.
摘要翻译：包括以下步骤：提供含有核酸，含有可检测标记的标记试剂和至少一种多价金属阳离子在基本水溶液中的混合物的步骤; 将混合物中的核酸化学碎裂以产生多种核酸片段; 以及将至少一个标记附着到至少一个所述核酸片段以产生可检测地标记的核酸片段。

23. 发明授权

US06489114B2 Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby 有权
标题翻译：用于标记核糖核酸的方法和由此获得的标记的RNA片段
公开(公告)号：US06489114B2
公开(公告)日：2002-12-03
申请号：US09737761
申请日：2000-12-18
申请人： Ali Laayoun , Duc Do , Charles G. Miyada
发明人： Ali Laayoun , Duc Do , Charles G. Miyada
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C12Q1/6806 , C12Q2563/131
摘要： A process is provided for labeling with signal amplification a ribonucleic acid (RNA), comprising fragmenting the RNA to form RNA fragments, fixing a first ligand to a terminal phosphate located at least one of the 3′ end and the 5′ end of each of a plurality of the RNA fragments, the terminal phosphate having been released during the fragmentation, and binding a plurality of labeling agents to the first ligand on each of a plurality of the fragments.
摘要翻译：提供了用信号扩增标记核糖核酸（RNA）的方法，其包括将RNA片段化以形成RNA片段，将第一配体固定在位于每个的3'末端和5'末端中的至少一个末端磷酸酯多个RNA片段，末端磷酸酯在片段化期间被释放，并且将多个标记试剂与多个片段中的每一个上的第一配体结合。

24. 发明授权

US08637322B2 Method for labeling or treating a biological sample containing biological molecules of interest, in particular nucleic acids 有权
标题翻译：用于标记或处理含有感兴趣的生物分子，特别是核酸的生物样品的方法
公开(公告)号：US08637322B2
公开(公告)日：2014-01-28
申请号：US11919668
申请日：2006-05-31
申请人： Eloy Bernal-Mendez , Ali Laayoun , Lionel Menou
发明人： Eloy Bernal-Mendez , Ali Laayoun , Lionel Menou
IPC分类号： G01N33/48 , C07H1/00
CPC分类号： G01N33/58 , G01N33/532 , G01N33/54353 , Y10T436/11 , Y10T436/143333
摘要： The present invention relates to a method for labeling biological molecules of interest contained in a biological sample, consisting in: a) providing a reaction vessel, b) immobilizing capture molecules, which are capable of binding a label of the biological molecules of interest, on a solid support of the vessel, c) introducing the biological sample but also at least one label of the biological molecules of interest into said vessel and optionally any ingredient required for labeling or prelabeling the molecules of interest, d) incubating the vessel content and immobilizing the label which is not reacted with the molecules of interest by binding to the capture molecules, and e) using the labeled molecules of interest for subsequent steps. A method for treating a biological sample is also disclosed.Said invention is preferably used in a manual or automated method for purifying nucleic acids.
摘要翻译：本发明涉及用于标记生物样品中所含感兴趣生物分子的方法，其包括：a）提供反应容器，b）使能够将感兴趣的生物分子的标记结合的捕获分子固定在所述容器的固体支持物，c）将所述生物学样品引入所述生物样品，还将所述生物分子的至少一个标记物引入所述容器，以及任选的任何标记或预标记目的分子所需的成分，d）孵育所述容器内容物并固定通过结合捕获分子不与感兴趣的分子反应的标记，和e）使用目的标记的分子进行后续步骤。还公开了一种用于处理生物样品的方法。所述发明优选以用于纯化核酸的手动或自动化方法使用。

25. 发明申请

US20110091898A1 DETECTION PROBE 失效
标题翻译：检测探头
公开(公告)号：US20110091898A1
公开(公告)日：2011-04-21
申请号：US12996457
申请日：2009-07-03
申请人： Ali Laayoun , Eloy Bernal Mendez
发明人： Ali Laayoun , Eloy Bernal Mendez
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/703 , C12Q1/6818 , C12Q1/701 , C12Q2525/307 , C12Q2525/301
摘要： A nucleotide probe permitting the detection of nucleic acids and constituted of a labeled nucleotide strand having three fragments: a first fragment having a first closing sequence, a second fragment, all or part of which has a recognition sequence for the molecular recognition of the target nucleic acid and a third fragment having a second closing sequence, and at least two markers, one of the ends of the strand of the detection probe being free of any marker, in which, when the two closing sequences are hybridized together, the detection probe has a completely circular shape, the closing sequences thus maintaining the probe in a conformation that cannot be detected in the absence of the target nucleic acid.
摘要翻译：允许检测核酸并由具有三个片段的标记的核苷酸链构成的核苷酸探针：具有第一封闭序列的第一片段，其全部或部分具有用于靶核酸的分子识别的识别序列的第二片段酸和具有第二闭合序列的第三片段和至少两个标记，所述检测探针的链的末端之一没有任何标记，其中当两个封闭序列杂交在一起时，检测探针具有完全圆形的形状，因此关闭序列因此将探针保持在不存在靶核酸的情况下不能被检测的构象。

26. 发明申请

US20080032288A1 Labeling Reagents, Methods for the Synthesis of Such Reagents and Methods for the Detection of Biological Molecules 失效
公开(公告)号：US20080032288A1
公开(公告)日：2008-02-07
申请号：US10590973
申请日：2005-03-24
申请人： Ali Laayoun , Eloy Bernal-Mendez
发明人： Ali Laayoun , Eloy Bernal-Mendez
IPC分类号： C07H21/00 , C07C233/07 , G01N33/58
CPC分类号： C07D495/04 , Y10T436/13 , Y10T436/143333
摘要： The present invention relates to a temperature-stable labeling reagent of formula (0): in which: R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers interlinked by at least one multimeric structure, L is a linker arm comprising a linear chain of at least two covalent bonds and n is an integer equal to 0 or 1, R3 and R4 represent, independently of one another: H, NO2, Cl, Br, F, I, R2 —(L)n—Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR, —CO—NH—(CH2)3—(O—CH2—CH2)3-CH2—NH—R2, —CO—NH—(CH2)3—(O—CH2—CH2)4—CH2—NH—R2 with R=alkyl or aryl, A is a linker arm comprising at least one covalent double bond enabling the conjugation of the diazo function with the aromatic ring and u is an integer between 0 and 2, preferably 0 or 1, —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—, -Z-represents —NH—, —NHCO—, —CONH— or —O—, m is an integer between 1 and 10, preferably between 1 and 3, and p is an integer between 1 and 10, preferably between 1 and 3. The present invention also describes a method for the synthesis of said labels and also applications for the labeling of biological molecules, in particular of nucleic acids, with a labeling reagent bearing the diazomethyl function.The invention is particularly suitable for use in the field of diagnostics.

27. 发明授权

US06921640B2 Process for labeling a ribonucleic acid 失效
标题翻译：用于标记核糖核酸的方法
公开(公告)号：US06921640B2
公开(公告)日：2005-07-26
申请号：US10101362
申请日：2002-03-20
申请人： Ali Laayoun
发明人： Ali Laayoun
IPC分类号： C12N15/09 , C07H21/00 , C07H21/02 , C12Q1/68 , C07H21/04 , C12N1/20 , C12N15/00 , C12N15/63
CPC分类号： C07H21/00
摘要： The present invention relates to a process for labeling a synthetic or natural ribonucleic acid (RNA). It also relates to RNA fragments, which have been labeled by fragmenting the RNA to free a terminal phosphate of each fragment for further reaction, and labeling each fragment at the freed terminal phosphate which is located at the 3′ end and/or the 5′ end of each fragment of the RNA, and to the use of such RNA fragments, for example, in the field of medical diagnosis.
摘要翻译：本发明涉及用于标记合成或天然核糖核酸（RNA）的方法。它还涉及RNA片段，其已经通过片段化RNA来标记以释放每个片段的末端磷酸酯用于进一步的反应，并且在位于3'末端和/或5'端的释放的磷酸末端标记每个片段， RNA的每个片段的末端，以及例如在医学诊断领域中使用这样的RNA片段。

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